Objective To determine and compare the contents of total iridoids in valerian from different origins.Methods The total iridoids in valerian was detected by reaction with alkaline hydroxylamine-perchloric acid iron reagent,and the absorbance was measured at 522 nm by spectrophotometry. Results The content of valepotriate and its absorption were linear within the range of 0. 05-1. 40 mg·mL-1 . The average recovery was 98. 50%,and RSD was 1. 02%. There was significant difference between the contents of iridoid in valerian from different habitats. Conclusion This method is accurate and reliable for evaluating the quality of valerianand. The contents of valeriana is related to its habitats.

This study successfully established in v itro model of Alzheimerˊs disease ( AD ) in order to investigate effects and mechanisms on AD by Bushen-Huatan-Yizhi Recipe (BSHTYZR, replenishing kidney-essence and removing phlegm to benefit wisdom ) . The PC-12 cells affected by Aβ1-42 incubated were produced for neuron cell model of AD . Then , they were intervened by the serum containing BSHTYZR , which were compared to the serum containing indomethacin . The cell morphology was observed . The neuronal survival was assessed by counting MTT assay . The expression of APP mRNA was detected with RT-PCR . And the protective effect of BSHTYZR on neuronal cells and neurotoxic effects of anti-Aβ was observed . The results showed that 2 . 5 mmol/L Aβ1-42 can significantly decrease the cell viability . The RT-PCR was used on semi-quantitative analysis for expression of APPmRNA. It showed that expression of APP mRNA was increased in each group, and the BSHTYZR group can reduce the expression significantly ( P < 0 . 01 ) . It was concluded that the BSHTYZR method can protect nerve cells , suppress the expression of APP mRNA , and reduce the toxic effects .

Objective　To investigate neural functional recovery and intracranial vascular disease of acute cerebral infarct (ACI) patients complicated with polyvascular disease (PolyVD).Methods　Retrospective analysis was conducted on 83 ACI patients with monovascular disease and 68 ACI patients with polyvascular disease seeking hospitalization in the Neurology Department of the Third Affiliated Hospital of Jinzhou Medical University from January 2019 to September 2019.The changes of intracranial artery disease and neural functional recovery of the monovascular disease group and polyvascular disease group were analyzed,and multiple factors that affect neural function recovery were analyzed.Results　(1)Compared with the monovascular disease group,the PolyVD group was found with a higher percentage of poor neural functional recovery,lower recovery rate of neural functions,and a larger number of vessels associated with the intracranial disease.The differences were statistically significan（P<0.05）.(2)The number of vessels associated with the intracranial disease and the number of vascular beds were positively correlated（P<0.05）,while the neural functional recovery rate and the number of vascular beds were negatively correlated（P<0.05）.(3)Age,medical history of diabetes and stroke,and number of vascular beds were all independent risk factors of poor neural functional recovery.Conclusions　Polyvascular disease is an independent risk factors of poor neural functional recovery among ACI patients.The higher the number of vascular beds of the disease complicated with carotid atherosclerosis is,the higher the possibility of poor neural functional recovery will be,and the worse the intracranial artery disease will be.