Objective:To discuss the effects of BuShen capsule on sexual function of castrated rats model.Methods:The castration model animals set up by removing testicle were divided into 5 groups,the large,middle and small-dose BuShen capsule groups,Nanbao capsule group,saline group and blank control group.At the end of the experiment,change of the latency time of penis erection,the incubation period and the number of their seizing of the female rats and the coefficient of the penis,the foreskin gland,seminal vesicle,prostate,and levator ani muscle in rats were determined.Histological section was made and changes in form of organization were observed under the light microscope,the levels of serum testosterone and cortisol in the rats were measured by radioimmunoassay.Result:Large,middle and small-dose of BuShen capsule could shorten the penile erection latency of castrated rats model.Large and middle-dose could decurtate the latency of their seizing female rats,large-dose could increase the number of their seizing female rats,middle-dose could increase the number of their seizing female rats.Large and middle-dose could obviously raise the levels of serum cortisol,middle-dose could improve the coefficient of the levator ani muscle.Conclusion:BuShen capsule could encourage the secretions of prostatae and seminal vesicle,accrete the cavernous body of penis and promote the growth of levator ani muscle.BuShen capsule has the good effects of invigorating the kidney and strengthening yang.

Objective: To explore the protective effects of jujube polysaccharide on liver injury model mice induced by ethionine and paracetamol. Methods: Liver injury mice model was induced by gavage with ethionine suspension or intraperitoneal injection of parcharide. ALT levels in serum and liver histopathological changes were measured, the protective effect of jujube polysaccharide on liver injury model mice induced by ethionine or paracetamol was observed. Results: Mice liver injury model can be successfully established by gavage with ethionine or intraperitoneal injection of paracetamol. High and low-dose jujuba polysaccharide and the glucurolactone groups can significantly lower the ALT levels in serum and significantly improve pathological changes in liver injury model. Conclusion: Jujube polysaccharide had good protective effects on liver injury model mice induced by ethionine or paracetamol.

OBJECTIVE:To study the effects of total glucosides of Verbena officinalis on chronic nonbacterial prostatitis (CNP)in mice and its anti-inflammatory and analgesic effects. METHODS:60 mice were randomized into normal group(normal saline),model group(normal saline),positive group(Qianliekang tablet 1.5 g/kg)and total glucosides of V. officinalis high-dose, medium-dose and low-dose groups(0.2,0.1,0.05 g/kg),with 10 mice in each group. CNP model was induced in those groups ex-cept for normal group;mice were given relevant medicine once a day for consecutive 21 days since 8th day of modeling. The white blood cell count,lecithin corpuscle density and pathological changes of prostate samples were observed. 50 mice were randomly di-vided into model group(normal saline),positive group(Aspirin tablet 0.3 g/kg or Dexamethasone acetate tablet 0.01 g/kg)and to-tal glucosides of V. officinalis high-dose,medium-dose and low-dose groups(0.2,0.1,0.05 g/kg);they were given relevant medi-cine intragastrically once a day for consecutive 7 days. The xylene-induced ear swelling test (Aspirin tablet as positive drug) and cotton-pellet granuloma test (Dexamethasone acetate tablet as positive drug) were conducted to determine the the degree of ear swelling and the net weight of granuloma. The acetic acid writhing test and hot plate test(Aspirin tablet as positive drug)were car-ried out to determine the latency period of writhing,the times of writhing within 20 min and pain threshold. RESULTS:Compared with model group,white blood cells count of prostate tissue were lowered while the density of lecithin corpuscles were increased significantly in medicine groups;the pathology of prostate tissue were improved significantly in positive group and total glucosides of V. officinalis high-dose and medium-dose groups,and mostly were 0 and Ⅰ grade. The degree of ear swelling and the net weight of granuloma were markedly reduced in positive group and total glucosides of V. officinalis high-dose and medium-dose groups;the times of writhing within 20 min decreased in medicine groups,and the latency period of writhing prolonged and pain threshold in-creased in positive group and total glucosides of V. officinalis high-dose group. All of the results showed statistical difference(P<0.05 or P<0.01). CONCLUSIONS:Total glucosides of V. officinalis can improve CNP in mice,and shows good anti-inflammatory and analgesic effects.

Objective To explore the effect of curcumine on rat cerebral ischemia model of blood stasis.Methods The blood-stasis rat model was established by injection of dexamethasone,then was given gastric infusion curcumine suspension for 10 continuous days.One hour after administration on the 11th day,we made ligation of the bilatory common carotid arteries of rats for 30 min to induce cerebral ischemia rat model.The rat blood sample was used for the detection of whole blood viscosity,and the brain was taken out for the observation of cerebral homogenate ATP and the pathological changes of brain tissue.Results The blood-stasis rats model was established successfully by intramuscular injection of dexamethasone,and cerebral ischemia rats model was established successfully by ligation of bilateral common carotid artery.Compared with the model group,curcumine could significantly reduce the whole blood viscosity of blood-stasis rats model of cerebral ischemia,and significantly increase the activity of Na+-K+-ATP,Mg2+ATP,Ca2+-ATP and Ca2+-Mg2+-ATP in brain homogenate,significant relieve the atrophy of nerve cells and glial cells of the model rats.Conclusion Curcumine can significantly improve the whole blood viscosity,ATP activity in the brain homogenate and morphological changes of brain tissue in rats model of blood stasis.

Objective To investigate the possibility of taking bees as laboratory animals and establishing the new rapid ag-ing animal model. Methods The bee model was established under environment of high temperature, lack of oxygen, and vibration. The appropriate conditions for establishing the model were investigated by observing the changes of appearance and measuring the activities of the total SOD and GSH-PX in the whole homogenate of bees. Meanwhile, the changes of biopsy were observed. Results Bees aging model could been set up at the conditions of appropriate temperature, hypoxia and noise. The model has obvious indications of aging, such as reduced activity of SOD and GSH-PX, and obviously pathological changes of brain organization and intestinal tissue. The aging indications are not the most seriously and are suit-able for the studies on drug improving the aging indications. Conclusion Being in the enviroment of high temperature (35 ℃), hypoxia and noise for4 hours, can result in the better bee aging model.

Objective:To explore the mechanism and characteristics of total saponin of stibmata maydis in decreasing blood glucose by study the effect total saponin of stibmata maydis on carbohydrate tolerance reducing mice model and ?-glucosidase activity in vitro.Methods:The model was established by intramuscular injecting Dexamethasone.Fasting blood sugar and serum blood sugar level were measured to observe the effect of total saponin of stibmata maydis.An enzyme-inhibitor model was established to study the effect of total saponin of stibmata maydis on ?-glucosidase activity.Results:Compared with model group,total saponin of stibmata maydis of middle dosage can remarkably reduce blood sugar level after irrigating the glucose for 30min,60min and 20min;high and low dose of total saponin of stibmata maydis and the group of Diamicron can obviously reduce the blood glucose level at every time period;total saponin of stibmata maydis can inhibit the activity of ?-glucosidase in vitro and have obvious dose-effect relationship.Fifty percent of ?-glucosidase activity is inhibited at 45.07?l(20mg/ml).Conclusion:Total saponin of stibmata maydis had the remarkable function of improving carbohydrate tolerance on reducing mice model induced by Dexamethasone and inhibits ?-glucosidase activity.

Objective:To observe the effect of Schisandra Chinensis Baill distilled by ethanol on energy metabolism in brain of repeated cerebral ischemia-reperfusion model mice.Method:The mice were randomly divided into six groups,respectively irrigating the groups of Nimodipine suspension,ginkgo leaf suspension,and large and small doses of Schisandra Chinensis Baill distilled by ethanol,sham group(blank) and the model group for the same volume of saline,once a day,15 days for delivery.Model mice of cerebral ischemia-reperfusion injury was established by bilateral common carotid artery blocking.48 hours after operation,the rats were decapitated,weight half of the brain and put 9 times saline into it,then the level of LD,vitality of LDH,AchE and ATP were measured.Results:Compared with model group,small doses of Schisandra Chinensis Baill distilled by ethanol could markedly reduce the content of LD of mice of repeated cerebral ischemia-reperfusion,large doses of Schisandra Chinensis Baill distilled by ethanol could notably improve the vitality of LDH;large and small doses of Schisandra Chinensis Baill distilled by ethanol could both notably improve the vitality of AchE,large doses of Schisandra Chinensis Baill distilled by ethanol could notably improve the vitality of Na+-K+-ATP、Ca2+-ATP.Conclusion:Schisandra Chinensis Baill distilled by ethanol could markedly improve the energy metabolism of the model of repeated cerebral ischemia-reperfusion of mice.

Objective To investigate the ischemic preconditioning time for inducing rat brain ischemia tolerance.Methods Procerebrum ischemic preconditioning injury was caused by occlusion of two-side common carotid artery for 10 minutes or 20 minutes,and subsequent cerebral ischemic injury was caused by occlusion of middle cerebral artery for 2 hours after 72 hours of reperfusion.The percentage of brain infarct size was calculated for the investigation of the proper ischemic preconditioning time for rat brain ischemia tolerance.Results Ischemic preconditionaing for 10 minutes and 20 minutes can reduce the percentage of brain infarct size significantly.The difference of the percentage of brain infarct size between 10-minute preconditioning group and 20-minute preconditioning group is insignificant,but the mean percentage of brain infarct size in 10-minute preconditioning group is less than that in 20-minute preconditioning group.Conclusion Ten minutes are the suitable time of ischemic preconditioning for rat brain ischemia tolerance.

OBJECTIVE: To observe the effects of Huatan Xiezhuo Recipe (HTXZR) on fatty liver of rats. METHODS: Forty-eight SD rats were randomly divided into five groups: normal control group, untreated group, Dongbao Gantai Tablet (DBGTT)-treated group, low-dose HTXZR-treated group and high-dose HTXZR-treated group. Fatty liver was induced in the rats by hyperlipid diet and intraperitoneal injection of tetracycline. The pathological changes of liver tissues in rats were observed, and the liver function, serum leptin, insulin resistance index, triglyceride (TG) and free fatty acid (FFA) in the liver were detected. RESULTS: The levels of serum leptin, insulin resistance index, FFA and TG in rats of the three treated groups were lower than those of the untreated group (P<0.01), and such effects in the HTXZR-treated groups were more significant than those in the DBGTT-treated group (P<0.05). CONCLUSION: The main action mechanisms of HTXZR in treating fatty liver are probably to promote the lipid metabolism, decrease the leptin and insulin resistance.

Objective To optimize the extraction process f or polysaccharide in the residue of Fructus Jujubae(FJ ).Methods The conditions of extraction process for polysaccharide of FJ was screen ed with the yield and content of polysaccharides as markers by orthogonal experimental design.Results The optimum extraction process was:soaking the residue of FJ with water at 90℃for3times(5hours each time )and precipitated with 80%ethanol.T he yield and content of polysaccharides were 20%and 43.54%respectively.Conclusion This procedure is reliable and polysac-charides can be extracted completely by this procedure.It can bring a hig h economy benefit.