Objective To analyze the emergency treatment protocols and nursing measures for patients with diquat and paraquat poisoning,aiming to provide references for updating future clinical practice.Methods A retrospective study was conducted involving 53 patients with diquat and paraquat poisoning admitted to the department of emergency of the First Affiliated Hospital of Wenzhou Medical University from January 1,2019,to December 31,2023.The patients were divided into survival and death groups based on their prognosis.Clinical data were collected to compare organ dysfunction,the proportion of hemoperfusion(HP),average number of HP sessions,the proportion of blood purification,average duration of blood purification,and the proportion of HP combined with continuous renal replacement therapy(CRRT)between two groups of patients with different prognoses.Results Among the 53 patients,27(50.94%)were male and 26(49.06%)were female;with an age range of 14 to 86 years and a mean age of(38.13±19.68)years.Fifty-two cases were due to intentional ingestion,and 1 was accidental.The detected blood concentrations of diquat ranged from 57.38 to 119762.00 μg/L,while those of paraquat ranged from 60.12 to 71 244.89 μg/L.Forty patients developed multiple organ dysfunction syndrome(MODS),with 38 ultimately progressing to multiple organ failure,primarily affecting the gastrointestinal tract,kidneys,and liver.After aggressive treatment and nursing,the blood concentrations of 13 patients(24.53%)dropped below 50 μg/L,and they were discharged after 4 to 34 days of hospitalization.Thirty-two patients'families opted for withdrawal of treatment and discharge,with subsequent confirmation of death after follow-up,hospital stay:1-4 days.Eight patients died in-hospital,hospital stay:1-3 days,resulting in a total mortality rate of 40 cases(75.47%).Compared to the survival group,the death group had significantly higher rate of neurological,renal,respiratory,and liver injuries[neurological:90.00%(36/40)vs.15.38%(2/13),renal:95.00%(38/40)vs.69.23%(9/13),respiratory:97.50%(39/40)vs.30.77%(4/13),liver injury:85.00%(34/40)vs.46.15%(6/13),all P<0.05].Furthermore,the death group had significantly lower average number of HP sessions and average duration of blood purification compared to the survival group[average number of HP sessions:4.35±2.42 vs.6.62±1.17,average duration of blood purification time(days):1.53±1.09 vs.5.23±3.90,both P<0.05].Conclusions Poisoning with a mixture of diquat and paraquat is life-threatening and associated with a high mortality.In addition to systematic treatment,individualized and dynamic nursing support should be provided,including close monitoring of the manifestations and laboratory indicators of affected organ systems.Therefore,optimization treatment protocols during the peak mortality period may help reduce mortality in patients with diquat and paraquat poisoning.

Recently, small nucleolar RNAs (snoRNAs) have transcended the genomic "noise" to emerge as pivotal molecular markers due to their essential roles in tumor progression. Substantial evidence indicates a strong association between snoRNAs and critical clinical features such as tumor pathology and drug resistance. Historically, snoRNA research has concentrated on two classical mechanisms: 2'-O-ribose methylation and pseudouridylation. This review specifically summarizes the novel regulatory mechanisms and functional patterns of snoRNAs in tumors, encompassing transcriptional, post-transcriptional, and post-translational regulation. We further discuss the synergistic effect between snoRNA host genes (SNHGs) and snoRNAs in tumor progression. More importantly, snoRNAs extensively contribute to the development of tumor cell resistance as oncogenes or tumor suppressor genes. Accordingly, we provide a comprehensive review of the clinical diagnosis and treatment associated with snoRNAs and explore their significant potential as novel drug targets.

Peptides that are composed of dextrorotary (d)-amino acids have gained increasing attention as a potential therapeutic class. However, our understanding of the in vivo fate of d-peptides is limited. This highlights the need for whole-body, quantitative tracking of d-peptides to better understand how they interact with the living body. Here, we used mouse models to track the movement of a programmed death-ligand 1 (PD-L1)-targeting d-dodecapeptide antagonist (DPA) using positron emission tomography (PET). More specifically, we profiled the metabolic routes of [⁶⁴Cu]DPA and investigated the tumor engagement of [⁶⁴Cu/⁶⁸Ga]DPA in mouse models. Our results revealed that intact [⁶⁴Cu/⁶⁸Ga]DPA was primarily eliminated by the kidneys and had a notable accumulation in tumors. Moreover, a single dose of [⁶⁴Cu]DPA effectively delayed tumor growth and improved the survival of mice. Collectively, these results not only deepen our knowledge of the in vivo fate of d-peptides, but also underscore the utility of d-peptides as radiopharmaceuticals.

Recurrent laryngeal nerve (RLN) injury is a serious complication of thyroidectomy. Intraoperative neuro-monitoring(IONM) is a technology for identifying the integrity of nerves during operation by electrophysiological means, which included the intermitted IONM and the continuous IONM.This paper aims to compare the superiorities and defects of both IONM methods and summarize the current state and progress of IONM.

The protection of recurrent laryngeal nerve in thyroidectomy has been a research topic for clinicians. High-risk thyroidectomy is an operation with high rate of recurrent laryngeal nerve injury which including thyroid reoperation, giant goiter surgery, substernal goiter surgery, dorsal thyroid tumor surgey and thyroid malignancy surgery and so on. The application value of three kinds of laryngeal recurrent nerve protection techniques during high-risk thyroidectomy will be reviewed in this article, such as visualization alone, intraoperative nerve monitoring and continuous intraoperative nerve monitoring.

Objective To explore the characteristics of cognitive function in patients with the first-episode and chronic schizophrenia. Methods 83 cases of first-episode schizophrenia patients, 77 cases of chronic schizophrenia patients and 75 healthy volunteers from January 2013 to June 2014 were selected. MATRICS consensus cognitive battery (MCCB) test was conducted on them and their cognitive profile patterns and characteristics of cognitive function impairment were analyzed. Results Compared with the healthy control group, the first-episode schizophrenia patients and chronic schizophrenia patients got hurt in psychomotor speed, attention, working memory, verbal learning and memory, visual learning and memory, reasoning and problem solving ability, social cognition and other areas in the MCCB (P<0.05). There were differences in comparing working memory and social cognition of the first-episode schizophrenia patients and chronic schizophrenia patients (P<0.05). Conclusion The first-episode schizophrenia patients get less injury than chronic schizophrenia patients in the working memory and social cognition. Whether first-episode schizophrenia pa-tients or chronic schizophrenia patients, they are impaired compared with the normal population in cognitive function.

OBJECTIVETo establish a long-standing cell line of esophageal squamous cell carcinoma (ESCC) in pursuit of a model for in vitro study of carcinogenesis.

METHODSSmall tissue blocks taken from resected specimens of esophageal cancer were cultured, and cell line EC9706 was established. The biologic properties of EC9706 were characterized. Comparative genomic hybridization(CGH) was performed on the cell line.

RESULTSThe growth curve of EC9706 was detected. The cell generation time was 26 hours. The plate colony forming efficiency is 91.9%, with the capacity of forming clones in soft agar. EC9706 cells show high tumorigenecity as indicated by the rapid regeneration of moderate-poor-differentiated squamous cell carcinomas after injection into nude mice. CGH analysis indicated copy number gains of 1p1, 1q2-4, 2p1, 2q1, 5p, 7p14, 7q21, 11q1, 15q2, 20q and losses of 2p2, 2q2, 3p, 4, 9p, 14, 18, Xq. High-level gain of 5p was observed.

CONCLUSIONEstablished cell line EC9706 can serve as a useful tool for studying the carcinogenesis of ESCC.

Aged ; Animals ; Carcinoma, Squamous Cell ; genetics ; pathology ; Cell Division ; Chromosome Aberrations ; Esophageal Neoplasms ; genetics ; pathology ; Humans ; Male ; Mice ; Mice, Nude ; Neoplasm Transplantation ; Nucleic Acid Hybridization ; methods ; Transplantation, Heterologous ; Tumor Cells, Cultured

Objective To observe the change of perioperati ve endotoxin (ET) level and renal function in obstructive jaundice (OJ) patient and the effect of sodium cholate,lactulose, and anisodamine.Methods Forty-eight OJ patients were randomly divided in to control group(n=15), sodium cholate treatment group(n=11), lactul ose treatment group (n=10) and a anisodamine treatment group(n=12), 21 patients with cholecystolithiasis served as non-jaundiced control group. The levels of plasma ET and endogenous creatinine clearance rate (Ccr) were determi ned in all the cases.Results Compar ed with non-jaundiced control group, plasma ET level increased significantly an d Ccr significantly decreased in OJ group (P

0.05) while the level was significantly higher in the former 4 groups than in the control (P

Objective: To study the plasma endotoxin(ET)on renal damage and clinical interference of anisodamine in patients with obstractive jaundice(OJ).Methods: Thirty patients with OJ were randomly divided into general treatment group(A group) and anisodamine treatment (B jroup).The level of plasma ET and endogenous creatinine clearance rate(Ccr) were determined at perioperative period,other 21 cases of chronic choelithiasis without jaundice were employed to serve as the control.Results: (1)The plasma ET level of peripheral blood was increased significantly and Ccr level was decreased markedly in both A and B group than in the control at lst day of hospitalization(P