AIM:To establish the fingerprint for Cacumen Platycladi (carbonized) by HPLC. METHODS:The column of Lichrospher C 18 (250 mm?4.6 mm 5 ?m)was used. The mobile phase consisted of 0.5‰ trifluoroactic acid-methanol with gradient elution. The detective wavelength was at 375 nm,and the flow rate was 1.0 mL/min. Different habitats were compared by Similarity Evaluation System for Chromatographic Fingerprint of CMM Version 2004A. RESULTS:The fingerprint consisted of 14 common peaks. The mutual mode of HPLC fingerprints was set up and the similarity of the crude drugs was in the range of 0.178-0.963. The standard HPLC fingerprint of Cacumen Platycladi (carbonized) was established too. CONCLUSION:This method is accurate and reliable and provides a scientific basis for the quality control of Cacumen Platycladi (carbonized).

OBJECTIVEThe spikes of Schizonepeta tenuifolia from different habits were predicted by UV-Vis spectrum.

METHODThe dimensions of spectrum data obtained from ten habits were reduced by principal component analysis, and the first six new variables with 99.82% of cumulative reliability were put into the backpropagation neural network for model building.

RESULTThe recognition rate of backpropagation neural network coupled with principal component analysis (PCA-BPNN) was 100%, and its mean square error was 0.001 0.

CONCLUSIONPCA-BPNN can be used for the classifying of spikes of S. tenuifolia from different producing area, and this method is simple and fast.

China ; Consumer Product Safety ; Lamiaceae ; chemistry ; Neural Networks (Computer) ; Principal Component Analysis ; Quality Control ; Spectrophotometry, Ultraviolet ; methods

OBJECTIVETo study the absorption kinetic characteristics of mollugin and purpurin in each intestinal segment of rats.

METHODThe in situ single-way perfusion rat model was established to study absorption characteristics of mollugin and purpurin in each intestinal segment of rats. The volume of recirculation fluid was regulated by phenol red.

RESULTDifferent quality concentrations (12.33, 24.66, 49.32 mg x L(-1)) of mollugin and (8.455, 16.91, 33.82 mg x L(-1)) purpurin showed a concentration gradient of absorption dose in each intestinal segment, with the osmotic coefficient increasing to more than 0.2 x 10(-4) cm x s(-1). In the same concentration, mollugin and purpurin showed an identical trend of P(eff) in each intestinal segment in the order of colon > duodenum > ileum > jejunum, with a significant difference (P < 0.05).

CONCLUSIONMollugin and purpurin are highly permeable in rat intestinal segments, with absorption in each segment, while the specific absorption existed in the colon segment.

Animals ; Anthraquinones ; metabolism ; Female ; Intestinal Absorption ; Male ; Pyrans ; metabolism ; Rats ; Rats, Sprague-Dawley ; Reproducibility of Results

OBJECTIVETo determine the equilibrium solubility of schizonepetin and its partition coefficients in the n-octanol-water/buffer solution systems.

METHODThe HPLC method was established and used to detect the concentration of schizonepetin in water and different organic solvents; the partition coefficients in the n-octanol-water/buffer solution systems of schizonepetin were determined by shaking flask method.

RESULTThe equilibrium solubility of schizonepetin was 910 mg x L(-1) in water at 25 degrees C. A higher equilibrium solubility of schizonepetin was reached at 93 855 mg x L(-1) in methanol. Partition coefficients in the n-octanol-water/buffer solution systems of schizonepetin was 49.35 (lnKow = 1.69) at 25 degrees C.

CONCLUSIONSchizonepetin was almost insoluble in petroleum ether. It has a low solubility in water balance. Equilibrium solubility in methanol is higher than what in other solvents. It have little change in apparent distribution coefficient in neutral phosphate buffer solution and has significant decrease in apparent distribution coefficient under the alkaline conditions.

Drugs, Chinese Herbal ; chemistry ; Kinetics ; Lamiaceae ; chemistry ; Oils ; chemistry ; Solubility ; Water ; chemistry

OBJECTIVETo establish a baking method of Platycladi Cacumen Carbonisatum for providing a new idea to Carbonic Herbs' research.

METHODSamples were prepared in an oven for different time at different temperatures separately. Then the fingerprints of the samples were determined by UPLC. According to the standard fingerprint, the similarities of the samples' fingerprints were compared.

RESULTThe similarities of 3 samples, which were baked at 230 degrees C for 20 min, 30 min and at 240 degrees C for 20 min, were above 0.96.

CONCLUSIONAccording to the similarities of the fingerprints and in view of the appearances, Platycladi Cacumen Carbonizing should be baked at 230 degrees C for 20 min.

Chromatography, High Pressure Liquid ; Cupressaceae ; chemistry ; Drugs, Chinese Herbal ; analysis ; Plant Extracts ; analysis ; Technology, Pharmaceutical ; methods

OBJECTIVETo investigate the flavonoids in Platycladi Cacumen.

METHODThe constituents in Platycladi Cacumen were determined by UPLC-MS. A Waters BEH C18 column (2.1 mm x 150 mm, 1.7 microm) was used with a gradient elution of methanol-water containing 0.2% formic acid. The mass spectrometer equipped with electrospay ionization source was used as defector and operated in data was collected under the negative ion modes.

RESULTEleven constituents were identified.

CONCLUSIONIn this study, the main flavonoids in Platycladi Cacumen were separated by UPLC, and identified through the information of mass number and UV spectra. With the information of MS/MS from flavonoids, the modes of breaking up flavonoids were discussed. It is an accurate and effective method which can be applied for the constituent identification of Platycladi Cacumen.

Chromatography, Liquid ; methods ; Cupressaceae ; chemistry ; Flavonoids ; chemistry ; Mass Spectrometry ; methods ; Plant Extracts ; chemistry