Objective To study the protective effect of Nimodipine treatment of brain damage in patients with cerebral hemorrhage.Methods 62 cases of brain damage in patients with cerebral hemorrhage were divided into treatment group and control group,each for 31 cases.The control group were given conventional treatment,and the treatment group were given Nimodipine on the basis of the control group,treatment for 10d.NIHSS,hematoma volume and clinical effective rate were compared.Results After treatment,the NIHSS was lower between two groups,the treatment group decline was more obvious(P ＜ 0.05).The hematoma volume was decreased,the treatment group decrease was more obvious(P ＜0.05).The total effective rate was 51.6％ in the control group,and the total effective rate was 77.4％.There were significantly statistical differences between two groups(P ＜ 0.05).Conclusion Nimodipine had a protective effect for brain damage in patients with cerebral hemorrhage,and could improve the nervous function and decrease hematoma volume,and it was a reliable drug.

Objective To study the effects of ganoderma spore oil on Friend murine leukemia virus(Fr.MuLV)and its mechanism.Methods The BALB/C female mouse was infected with Fr.MuLV,body weight,spleen index,thymus index,the percentages of CD4+,CD8+,and CD4+/CD8+ were mea-sured.Results Compared to the control group,body weight extenuation,splenomegaly,and atrophy of thymus gland severely in the model group,the percentages of CD4+,CD8+,and CD4+/CD8+ were significantly decreased in the model.Body weight,spleen index,and thymus index(P

Objective To discuss the influence of anatomical variations of the cystic duct on preoperative diagnosis and operational scheme for cholecystectomy. Methods A 47-year-old woman was admitted to our hospital with diagnosis of cholecystolithiasis. Ultrasonography suggested minimal intra- and extrahepatic ductal dilatation. Laboratory tests showed that serum levels of alanine aminotransferase, aspartate aminotransferase and alkaline phosphatase were 189 IU/L, 366 IU/L and 144 IU/L, respectively. In order to make a certain diagnosis, the patient received both magnetic resonance cholangiopancreatography (MRCP) and endoscopic retrograde cholangiopancreatography (ERCP). Results MRCP showed the bile duct slightly dilated with a shuttle shape figure and a lower signal with a strip form in it. MRCP could not confirm the quality of this signal and was doubtful of choledochus diaphragma. Subsequently, ERCP was applied to demonstrate that the cystic duct was collateral with the common hepatic duct when arriving into its left side and converged into the bile duct with a lower position, which was the reason for why MRCP misjudged the formation of choledochus diaphragma in the bile duct. Finally, the patient underwent open cholecystectomy. Conclusion There are some kinds of variations in the cystic duct including course, appearance and location of confluence. Combing MRCP with ERCP can significantly elevate the diagnostic accuracy of the cystic duct before operation, especially in those patients with doubtful diagnosis upon admission. To avoid biliary injury as much as possible, open cholecystectomy is superior to the laparoscopic cholecystectomy (LC)with regard to the patients suffering from cholecystolithiasis complicated with variation of the cystic duct.

Objective To explore the features of white matter in DTI in patients with asymptomatic mild traumat?ic brain injury(mTBI)and their correlation with the cognitive features. Methods The DTI data and cognitive function data were obtained from 36 mTBI patients and 36 health controls. The fractional anisotropy (FA) pictures were ana?lyzed by voxel-based analysis. The FA value differences of the two groups were analyzed by using t test. The values of FA were extracted from the abnormal regions of mTBI patients. Correlation analyses were performed on the association of extracted FA value with P300 latency,P300 amplitude,the scores of Montreal Cognitive Assessment (MoCA) Scale, Hamilton Depression Scale, Hamilton Anxiey Scale,age and education(P>0.05). Results Compared with controls, mTBI patients had significant reduction of FA in the right medial frontal gyrus, bilateral postcentral gyrus, left precune?us, right posterior cingutate and right superior temporal gyrus(P<0.01,FDR correction). There were no significant correlations between extracted FA value and all clinical characteristics in mTBI patients. Conclusions Patients with mTBI have white matter microstructural damage and cognitive impairment.

Objective To investigate the efficacy and safety of covered stent in treatment of esophageal perforation. Methods Observe and analyzed the effect and complications of covered metallic stent in esophageal perforation. In all of 40 cases, the most common causes were iatrogenic, esophageal cancer, and foreign body. Results All patients with esophageal perforation got successful esophageal stent placement. All of them recovered smoothly in eating. The stents were removed 4 days to 1 month later. Gastroscopy and angiography confirmed esophageal perforation has healed. 3 cases of esophageal cancer patients with dysphagia obviously relieved after stent placement. The main complications in stent and stent migration for chest pain after operation. 6 cases (15 %) of patients with stent displacement, while 2 cases (5 %) of esophageal cancer patients with food obstruction and stent displacement. Conclusion Treatment of esophageal perforation with covered metal stents endoscopically is effective and safe.

Objective To evaluate the feasibilities and advantages of different concentrations of sodium alginate (SA) solutions as a submucosal injection solution for endoscopic submucosal dissection (ESD). Methods In vitro study, different concentrations of sodium alginate solutions and normal saline were injected into submucosal of resected porcine esophagus and stomach respectively, then observe and measure the heights of each injection induced mucosal elevations, and their changes over time. In vivo study, the mimic ESD were conducted in healthy pigs to evaluate the mucosal elevation effect and other assistant effects of sodium alginate as a submucosal injection solution. Results The elevation heights of the experiment groups injected with SA solutions were much higher than the control group injected with normal saline. Specially, the elevation created by 1 % SA in porcine esophagus was significantly higher than that of normal saline (P < 0.01) and the elevation created by 3 % SA was significantly higher than that of normal saline in porcine stomach (P < 0.001). In the mimic ESD experiment, mucosal elevation with clear margin occurred immediately after injection with SA solution. And the durable submucosal fluid cushion created by SA protected deeper tissues while facilitating ESD procedure. Conclusion The elevation heights created by SA solutions were greater and more durable than that created by normal saline, which were crucial for ESD. The viscosity property enabled SA to form a stable protective cushion and prevent bleeding by squeezing tissue around the wound, which may decrease perforation and bleeding rate during ESD procedure. Therefore, sodium alginate can be an ideal clinical submucosal injection solution.

OBJECTIVETo screen the anti-tumor active parts from Tripterygium hypoglaucum by anti-tumor experimental model in vivo and in vitro.

METHODEthanol extraction was separated and purified by column chromatography of ion polymeric adsorbent and macroporous adsorptive resins. MTT assay and the inhibition effect to S180 solid tumor were used to detect anti-tumor activity of each separation.

RESULTThere are anti-tumor activities in the ethanol extraction, total alkaloids, and macroporous resin absorption in vivo and vitro. Minimum IC50 of Total alkaloids was 29.90 mg L(-1), and S180 solid tumor inhibition ratio of different dose of 25, 50, 100 mg kg(-1) were 38.10%, 50.60%, 60.71% respectively. Minimum IC50 of macroporous resin absorption was 98.56 mg L(-1), and S180 solid tumor inhibition ratio of different dose of 100, 200, 400 mg kg(-1) were 42.96%, 53.57%, 63.79% respectively. Water solubility position had no effect in vivo and vitro.

CONCLUSIONT. hypoglaucum has fine anti-tumor activity in vivo and in vitro, and total alkaloids are the main part of anti-tumor active part.

Alkaloids ; isolation & purification ; pharmacology ; Animals ; Antineoplastic Agents, Phytogenic ; isolation & purification ; pharmacology ; Cell Line, Tumor ; Cell Proliferation ; drug effects ; Drug Screening Assays, Antitumor ; Drugs, Chinese Herbal ; isolation & purification ; pharmacology ; Humans ; Inhibitory Concentration 50 ; Mice ; Tripterygium ; chemistry

Objective To evaluate the changes in motor function impairment and brain functional networks of patients with acute ischemic stroke(AIS)through parameters of spontaneous activities of both upper limbs and electroencephalogram graph theory analysis methods.Methods The data of 34 acute ischemic stroke patients(observation group)with upper limb motor disorders who were treated in the Department of Neurology of the Affiliated Hospital of Southwest Medical University from January 2022 to October 2023,and 40 healthy controls(HC group)were collected.The subjects completed the National Institutes of Health Stroke Scale(NIHSS)and Fugl-Meyer Assessment(FMA)within 7 days,and wore wrist activity recorders(Actiwatch)continuously for 24 hours to collect data on sponta-neous activities of upper limbs and analyzed related parameters such as the coordination coefficient of both upper limbs(r),the activity ratio of the affected side to the healthy side upper limb(ULAR),etc.At the same time,all subjects completed approximately 2 hours of 19-channel electroencephalogram examination.After preprocessing the electroencephalogram data,5 segments of 10-second resting-state electroencephalogram were extracted for graph theory.Results ① Compared to healthy individuals,AIS patients exhibited decreased functional connectivity edges in the 8 and θ bands,with substantial reductions in network connections in the α band.In the β band,connections between the frontal,right parietal,and occipital regions weakened,while connections from the right temporal lobe to the left temporal lobe strengthened.In the γ band,there was a significant increase in connections throughout the brain.② Graph theory analysis revealed significantly increased shortest path lengths(α band:t=2.228,P＜0.05,d=-0.52;β band:t=-3.641,P＜0.01,d=-0.878)and decreased global efficiency(α band:t=2.535,P＜0.05,d=0.591;β band:t=3.321,P＜0.01,d=0.803)in the observation group compared to the control group.In the γ band,local effi-ciency(t=3.279,P＜0.01,d=0.765)and clustering coefficients were significantly higher(t=3.358,P＜0.01,d=0.783).③ In the-γ band,the ULAR ≤30％group showed significantly re-duced shortest path length(t=-2.063,P＜0.05,d=-0.802)and increased global efficiency(t=2.226,P＜0.05,d=0.865),local efficiency(t=2.95,P＜0.05,d=1.147),and cluste-ring coefficient(t=2.962,P＜0.05,d=1.148).④ In the observation group,the bilateral upper limb coordination coefficient during sleep was negatively correlated with NIHSS scores(r=-0.389,P＜0.05)and ULAR(r=-0.395,P＜0.05),while FMA scores were positively cor-related with ULAR(r=0.442,P＜0.05).Conclusion The parameters of spontaneous activities of the upper limbs can be used to determine the impairment of motor function in AIS patients.The combination of changes in brain functional networks and motor impairments can provide new ideas for the study of their neural network mechanisms.

Objective To evaluate the changes in motor function impairment and brain functional networks of patients with acute ischemic stroke(AIS)through parameters of spontaneous activities of both upper limbs and electroencephalogram graph theory analysis methods.Methods The data of 34 acute ischemic stroke patients(observation group)with upper limb motor disorders who were treated in the Department of Neurology of the Affiliated Hospital of Southwest Medical University from January 2022 to October 2023,and 40 healthy controls(HC group)were collected.The subjects completed the National Institutes of Health Stroke Scale(NIHSS)and Fugl-Meyer Assessment(FMA)within 7 days,and wore wrist activity recorders(Actiwatch)continuously for 24 hours to collect data on sponta-neous activities of upper limbs and analyzed related parameters such as the coordination coefficient of both upper limbs(r),the activity ratio of the affected side to the healthy side upper limb(ULAR),etc.At the same time,all subjects completed approximately 2 hours of 19-channel electroencephalogram examination.After preprocessing the electroencephalogram data,5 segments of 10-second resting-state electroencephalogram were extracted for graph theory.Results ① Compared to healthy individuals,AIS patients exhibited decreased functional connectivity edges in the 8 and θ bands,with substantial reductions in network connections in the α band.In the β band,connections between the frontal,right parietal,and occipital regions weakened,while connections from the right temporal lobe to the left temporal lobe strengthened.In the γ band,there was a significant increase in connections throughout the brain.② Graph theory analysis revealed significantly increased shortest path lengths(α band:t=2.228,P＜0.05,d=-0.52;β band:t=-3.641,P＜0.01,d=-0.878)and decreased global efficiency(α band:t=2.535,P＜0.05,d=0.591;β band:t=3.321,P＜0.01,d=0.803)in the observation group compared to the control group.In the γ band,local effi-ciency(t=3.279,P＜0.01,d=0.765)and clustering coefficients were significantly higher(t=3.358,P＜0.01,d=0.783).③ In the-γ band,the ULAR ≤30％group showed significantly re-duced shortest path length(t=-2.063,P＜0.05,d=-0.802)and increased global efficiency(t=2.226,P＜0.05,d=0.865),local efficiency(t=2.95,P＜0.05,d=1.147),and cluste-ring coefficient(t=2.962,P＜0.05,d=1.148).④ In the observation group,the bilateral upper limb coordination coefficient during sleep was negatively correlated with NIHSS scores(r=-0.389,P＜0.05)and ULAR(r=-0.395,P＜0.05),while FMA scores were positively cor-related with ULAR(r=0.442,P＜0.05).Conclusion The parameters of spontaneous activities of the upper limbs can be used to determine the impairment of motor function in AIS patients.The combination of changes in brain functional networks and motor impairments can provide new ideas for the study of their neural network mechanisms.

Objective:To design a knowledge-based cervical cancer planning model and apply it to cases of endometrial cancer and rectal cancer in order to explore the generalization of the model.Methods:A total of 179 cases of pelvic regions with different prescribed doses of dual-arc volumetric modulated arc therapy clinical plans were collected, of which 99 cases of cervical cancer clinical plans with a prescribed dose of 50.4 Gy were used as the training set to establish the RapidPlan model, and the remaining clinical plans were divided into 4 validation groups with 20 cases in each group. The clinical plans for cervical cancer and endometrial cancer with a prescription dose of 50.4 Gy were named groups A and B, while the clinical plan for endometrial cancer and rectal cancer with a prescription dose of 45 Gy were named groups C and D. The model was used to redesign the clinical plans in the 4 groups and the automatic plans were obtained. The planning target volume (PTV) and organ at risk (OAR) dosimetry parameters were compared between automatic plans and clinical plans.Results:The conformity index (CI) of the automatic plans in the A, B, C, and D groups were equivalent to that of the clinical plans ( P>0.05). The homogeneity index (HI) and D2% of the automatic plans in groups A, B, and C were all lower than those in clinical plans(HI, Z=-3.248, -3.360, -2.329, P<0.05; D2%, Z=-2.987, -3.397, -2.442, P<0.05). The HI and D2% of the automatic plans in group D were similar those in the clinical plans ( P>0.05). While ensuring the PTV coverage, the average value of OAR dosimetry parameters in all automatic plans groups were lower than that of the clinical plans. Conclusions:The RapidPlan model established by the cervical cancer clinical plans can complete the automatic plan design for endometrial cancer and rectal cancer under different prescription doses, which preliminarily proves the possibility of the generalization of the RapidPlan model.