Expression and regulation of the P-glycoprotein (P-gp) is associated with the development of multidrug resistance in cancer.Cancer cells are exposed in endogenous damages from its own micro-environments and inflicted stimulated damages.These harmful micro-environments could trigger cellular stress response and regulated the expression of P-gp through various activated signal transduction pathways.Researches of the cellular endogenous micro-environments and exogenous stimulation damages on the expression and regulation of P-gp would have important significance for exploring the multidrug resistance of cancer cell.

ObjectiveTo explore the clinical efficacy of Ningmitai capsule for treatment of patients with diabetic urinary tract infection.Methods A total of 100 diabetes patients with urinary tract infection from Zunyi First People's Hospital in Guizhou Province and Longkou Family Planning Service Station in Shandong Province wereenrolled in the study and randomly divided into control and treatment groups after they were informed and gave consent. With basic therapy to lower blood glucose, 65 cases in the treatment group were treated with Ningmitai capsule(4 granules/time, every grain of 0.38 g), while another 35 cases of the control group were given Sanjin tablets (4 granules/time, every grain of 0.29 g), 3 times a dayfor 2 weeks in both groups. Before and after treatment, the clinical effect, blood routine, urine routine, blood glucose, negative conversion ratio of midstream urine bacteria culture and tradition Chinese medicine syndrome curative effect in both groups were observed.Results The total effective rate of treatment group was significantly higher than that of the control group〔88.52%(54/61) vs. 70.59%(24/34),P<0.01〕. Before treatment, the differences in red blood cell(RBC) and white blood cell(WBC) count in urine samples as well as RBC, WBC, hemoglobin(Hb) and glucose in blood samples had no statistical significance in the comparisons between the two groups(allP>0.05). Compared with those before treatment, after treatment, the urinary RBC and WBC were decreased in treatment group, while in control group WBC was decreased but RBC increased, and in the comparisons of above indexes between the two groups there were statistical significant differences〔RBC(cell/μL): 1.99±0.59 vs. 9.11±3.76, WBC(cell/μL): 23.09±6.18 vs. 63.72±25.76, bothP<0.05〕. The blood RBC was significantly lowered after treatment in control group(×109/L: 3.93±0.40 vs. 4.02±0.39,P<0.05). In both groups, after treatment, WBC, Hb and glucose in blood were reduced in comparisons to those before treatment, and no statistical significant differences were found(allP>0.05). Negative conversion ratio of midstream urine bacterial culture in treatment group was significantly higher than that in control group〔80%(4/5) vs. 0(0/2)〕. Syndromes of traditional Chinese medicine were ameliorated at different degrees after treatment in both groups. The effectiveness of treatment group on urinary urgency, yellowish turbid urine and swelling pain in lower abdomen were obviously more valid than that of control group〔urinary urgency: 90.0%(54/60) vs. 61.8%(21/34), yellowish turbid urine: 75.0%(39/52) vs. 47.6%(10/21), swelling pain in lower abdomen: 74.5%(35/47) vs. 70.6%(12/17),P<0.05 orP<0.01〕, but other symptoms were similar in the two groups(allP>0.05).ConclusionThe clinical efficacy of Ningmitai capsule is better than that of Sanjin tablet for treatment of patients with diabetic urinary tract infection, and no adverse reaction has been seen in clinical observation.

ObjectiveTo observe the clinicalefficacies of ginger-partitioned moxibustion in three different periods, i.e. the canicular days, the Spring Equinox, and the Autumnal Equinox, in treating allergic Rhinitis.MethodEighty-nine patients with allergic rhinitis were elected and divided into3 groups by using the random number table (canicular group, Spring Equinox group, and Autumnal Equinox group), to receive the same treatment with same point selection. The change of serum IgE and improvement of symptoms were observed after the intervention in the three groups.ResultThe three groups all showed significant decrease of serum IgE and improvement in the symptoms of allergic rhinitis (P<0.05), and the therapeutic efficacy of the canicular group was markedly higher than that of the Spring Equinox group (P<0.05) and the Autumnal Equinox group (P<0.05).ConclusionThe canicular days are the optimal timeperiodfor the treatment of allergicrhinitis.

Objective To study the reconstructive treatment of the different types of neurofibroma and neurofibromatosis in different locations. Methods 37 patients with neurobibroma or neurofibromatosis were reviewed, including 8 cases with simple suture after excision, 9 cases with skin grafting in situ, 12 cases with skin flap grafting. The main points of the treating methods for different types of neurofibroma and neurofibromatosis were discussed, and the chief clinical manifestations, therapeutic principle and notices of the operation were then summarized.Results These 37 patients, including 25 cases of neurofibroma, 11 cases of neurofibromatosis typeⅠ and 1 case of neurofibromatosis type Ⅱ, were satisfied with the results after reconstructive operation. Conclusion The methods of reconstructive operation of neurofibroma and neurofibromatosis, such as simple suture after excision, skin grafting in situ, skin flap grafting, the use of skin soft tissue expansion, microskin grafting and so on, must be selected according to the different type and location of the neurofibroma and neurofibromatosis. The reconstructive operation must guarantee the best recovery of the visage and the function on the basis of the most degree of tumor excision.

Objective To establish a semi-quantitative method for measurement of HCV RNA by use of primers and probe, which was sensitive and designed by ourselves, a new europium fluorescent chelate BHHCT. Methods 44 serum samples of HCV infected patients and 20 samples of the healthy people were collected. HCV RNA in serum sample was extracted by HCV fluorescence PCR diagnostic kit produced by Zhongshan University DAAN Gene Co Ltd, and amplified by RT-PCR in which one PCR primer was pre-labeled with biotin. The amplified products were hybridized with capture probes pre-fixed on the microplate. The biotin in the amplified products was conjugated with europium labeled streptavidin. So europium was linked to the target DNA. Then the fluorescent of europium was measured. Results The linear range of this assay was 10 - 10 copies/ml. Both sensitivity and specificity were 100%. Conclusion Europium labeled RT-PCR assay is a sensitive, specific, fast and non-radioactive contaminant method for the measurement of HCV RNA.

005) There was significant difference between pre-operation and 56 th day( P

OBJECTIVE To investigate the risk factors and to take some useful measures to prevent and reduce infection in order to enhance medical quality,to ensure medical security,to strengthen hospital infection manangement and to prevent hospital infection effectively. METHODS We investigated the prevalence rate of hospital infection among our hospitalized patients in 2001,2003 and 2005, respectively. RESULTS The hospital infection rate was 4.6-6.42% in these years.Risk factors and the abuse of antibiotic were decreasing. CONCLUSIONS In order to control hospital infection rate,mensures should be taken including intensively monitoring the departments with high infection rate,strengthening hospital operation,rationally using the antibiotics,and studying the management for hospital infection.

Objective To establish the quality standard of Tiaojing Zhixue Granules.Methods Radix Astragali,Radix Paeononiae Alba,Herba Ecliptae and Fructus Psoraleae in Tiaojing Zhixue Granules were identified by TLC;Paeoniflorin content in Tiaojing Zhixue Granules was determined by HPLC.Results The relevant spots in Radix Astragali,Radix Paenoniae Alba,Herba Ecliptae and Fructus Psoraleae can be identified by TLC.The content of paeoniflorin in Radix Paenoniae Alba can be determined by HPLC.The linearity of paeoniflorin was good in the range of 0.0968 ~ 0.4838 ?g(r = 0.9999).The average recovery of paeoniflorin was 99.71 % with RSD = 1.33 %.Conclusion The established quality standard is simple,feasible and repeatable,and can be used for quality supervisory of Tiaojing Zhixue Granules.

AIM: To establish a method of determining the content of volatility contents in Baicao Oil. METHODS: The content of menthol, cinnamaldehyde, and eugenol were determined by gas chromatography. RESULTS: Menthol, cinnamaldehyde, and eugenol were well separated and the linearity was fine with the recovery in the range of 98%-102%. CONCLUSION: The method is rapid, stable and accurate. It can be used to control the quality of this preparation.

The aim of this study is to prepare self-microemulsifying drug delivery system (SMEDDS) of the mixture of paeonol (Pae) and borneol (Bor). Solubility test, ternary phase diagrams and simplex lattice method were employed to screen and optimize the formulation of the mixture of Pae and Bor-loaded SMEDDS. After formed into microemulsions, the particle diameter (PD) was determined and a TEM was employed to observe the microemulsions' morphology. The contents of Pae and Bor were determined by gas chromatography. As a result, while ethyl oleate (EO) as the oil phase, cremophor EL35 (EL35) as surfactant and Transcutol HP (HP) as cosurfactant, the range of the microemulsion on the ternary phase diagram was larger than other combinations. And at a ratio of 20:45:35, the microemulsions' PD was about 34 nm and the polydispersity index (PI) was about 0.2. There were 16% of Pae, 2% of Bor, 16% of EO, 37% of EL35 and 29% of HP in the prepared SMEDDS. The preparation process of the Pae and Bor-loaded SMEDDS based on Xingbi Fang is simple and feasible. This study provides a reference for the researches on the related traditional Chinese medicine and the related components.