Objective Aimed at the problem that there is no curative effect standard of liver-qi invasion of premenstrual syndrome (PMS), to construct the reference standard of curative effect evaluation for the liver-qi invasion of PMS. Method Curative effect evaluation research of Jingqianping granule was designed by the principle of random, double blind, contrast and multicentre. Three hundred and fifty-six patients with liver-qi invasion of PMS were divided into two groups randomly, one was Jingqianping granule, the other was placebo. Patients were given Jingqianping granule and placebo respectively for two menstrual cycles and followed–up for three menstrual cycles. The reference standard of curative effect evaluation for the liver-qi invasion of PMS was constructed with daily rating of severity of problems form (DRSP). Result Jingqianping granule and placebo have therapeutic effect on the patients with liver-qi invasion of PMS, and Jingqianping granule was better than placebo. DRSP can be used as indexes of curative effect evaluation. Conclusion The initial establishment of the curative effect evaluation criterion of PMS liver-qi invasion provides the important foundation for profession criterion’s establishment.

Objective To study the effect of synthetic HLA-derived peptide (P), HLA-B*2702.75-84, on the mean survival time (MST) of cardiac allografts in mice.Methods NIH mice cardiac allografts were heterotopically transplanted into the posterior of Balb/c ears. The HLA-derived peptide in combination with a subtherapeutic dose of CsA were perioperatively administrated. The pulsation of the cardiac allograft observed under the operating microscope was considered as the indication of the cardiac allograft surviving time or rejection. Results MST was ( 7.5? 0.5) days in untreated control group, ( 8.5? 1.5) days in CsA group and ( 7.0? 1.5) days in control peptide or P groups respectively, whereas MST was ( 26.5? 3.5) days in experimental group.Conclusion The synthetic HLA-derived peptide combined with subtherapeutic CsA can significantly prolong cardiac allograft survival in mice as compared with control groups.

Calcium/calmodulin-dependent protein kinaseⅡ (CaMKⅡ) has multiple functions, which made it play a central role in cardiovascular disease. Especially it activates numerous downstream targets in various signaling pathways that promotes vascular disease, heart failure, myocardial hypertrophy and arrhythmias. CaMKⅡcan impact calcium balance and increase calcium leak in myocardial cell via phosphorylating L type calcium channel, Ryanodine receptor (RyR 2) and phos?pholamban (PLN), and regulate ATP sensitive potassium current (IKATP) and late sodium current by affecting sodium channels and potassium channels. In addition, It can directly regulate transcription via activating the silk crack the original activated protein kinases (MAPKs) and acetylation enzyme (HDAC). These mechanisms have important roles in myocardial hypertro?phy, heart failure and arrhythmia. So we focus to demonstrating the structure and action mechanism of CaMKⅡto improve a new therapy of cardiovascular disease.

Hidden-pathogen theory is always a leading theory of febrile disease etiology from the proposal until the Ming Dynasty and it plays a critical guiding role on development and maturity of febrile disease theory. However, along with the change of times and drift of disease spectrum, this theory is facing unprecedented challenges. The gradually in-creased clinical experiences and facts have been difficult to be explained and induced using the already existed hidden-pathogen theory. Since hidden-pathogen theory has its own theoretical status and value, which cannot be lightly given up, the question of how to develop this theory in order to better reveal the pathogenesis in the guidance of diagnosis and treatment has become the focus of attention. This paper tried to proposeemotional hidden-pathogenas an example to provide a possible paradigm to develop hidden-pathogen theory and systematically expound main ideas and reasoning processes of emotional hidden-pathogen doctrine and to explore its theoretical significance and application value.

This study was aimed to reveal the roles of biological factors T3, T4 and TSH in the pathogenesis of liver-qi invasion syndrome and liver-qi depression syndrome of premenstrual syndrome (PMS) in the body. Thus, we may expound the connotation of conceptpotential stagnation ofqi and blood. Female workers of Jinan and Qingdao were selected as target groups by epidemiological cross-sectional survey. And specially trained investigators were responsible for screening patients referred to criterion on the international diagnostic standards and syndrome diagnosis of PMS. Patients' blood was taken as samples during the follicular phase (6 to 14 days), luteal phase (15 to 23 days), premenstrual phase (24 to 28 days) and the menstrual phase (1 to 5 days), respectively. Blood samples were disposed according to requirements of radioimmunoassay. Single factor analysis of variance was used in the comparison with the level of T3, T4 and TSH in menstrual blood for different time intervals of the case group and the normal group by SPSS 10.0 statistical software. The results showed that T3 levels of patients with either syndrome were significantly reduced during the premenstrual phase and the menstrual phase (P < 0.05). There were no significant changes on the level of serum T4 and TSH before and after the occurrence of the disease. Both of them were significantly increased during each phase of the menstrual cycle. It was concluded that changes of T3, T4 and TSH were one of the mechanisms in disease with liver-qi invasion syndrome and liver-qi depression syndrome. The micro change was the essence ofpotential stagnation of qi and blood, which was the body condition of this disease.

Objective To observe the protective role of enalapril as a specific angiotensin converting enzyme inhibitor on allograft in renal transplant recipients.Methods From Jan 2000 to Jun 2001,22 cases of renal transplant recipients with normal renal function and urine TGF-?_1 concentration being higher than 250.0 pg/mg Cr(group A) underwent therapy with angiotensin converting enzyme inhibitor(enalapril) one year after surgery.Enalapril was administered at a dose of 50 mg/d for the patients in group A for at least one year.Twenty-three recipients who never received angiotensin converting enzyme inhibitor in the same condition were studied as Group B.The adverse reactions of enalapril were investigated in group A and the expression of TGF-?_1mRNA in renal grafts were compared between before and 1 year after enalapril therapy.At the end of 3-year study period,the renal function,the decrement of creatinine clearance rate(Ccr) and the concentration of TGF-?_1 in blood and urine were compared between the two groups respectively.Results The Ccr decreased faster in group B than in group A.During three years study period,the decrements of Ccr were(5.1?4.6) and(13.7?9.5)(ml/min) in group A and group B respectively,and there were 2 cases and 9 cases with chronic allograft nephropathy(CAN) respectively.The decrement of Ccr and the number of CAN cases were significant difference between group A and group B(all P

BACKGROUND: Previous studies showed that donor systemic injection of B7/CD28 costimulatory blocker cytotoxic T lymphocyte associated antigen 4 immunoglobulin (CTLA-4Ig) needed in T cell activation can markedly prolong the survival time of rat renal allografts, which, however, has limitations, such as high dose, extensive influence, poor specificity, systemic adverse reactions.OBJECTIVE: In order to improve the targeting of CTLA-4Ig, we modified the dendritic cells of donors and recipients in vitro with CTLA- 4Ig and observed the influence of two kinds of dendritic cells applied alone or together on the survival of renal allografis in rats.DESIGN, TIME AND SETTING: The randomized controlled animal experiment was performed between April 2003 and July 2004 at Laboratory of Department of Urinary Surgery, Xinqiao Hospital, the Third Military Medical University, Chongqing, China.MATERIALS: Kidney donor: inbred Brown-Norway rats, kidney recipient: inbred Lewis rats, unrelated lymphocyte donor: Wistar rats.METHODS: Bone marrow derived dendritic cells of Lewis and Brown Norway rats were modified with CTLA- 4Ig gene recombinant adenovirus in vitro. Animal models of kidney transplantation were built with Brown Norway rats as donors while Lewis rats as recipients. The modified dendritic cells were injected into Lewis rats through femoral vein 24 hours before kidney transplantation alone (group 1 (n=8), donor dendritic cells; group 2 (n=8), recipient dendritic cells) and in combination (group 3 (n=8), donor and recipient dendritic cells). While the recipients without injection were used as control (group 4 (n=6)).MAIN OUTCOME MEASURES: Survival time of renal allografts; the reaction degrees of splenocytes to donor and unrelated antigen determined by MTT method on day 20 postoperation.RESULTS: Survival time of renal allografts in group 2 was not prolonged compared with group 4 while the survival time was markedly prolonged in group 3 (P < 0.01). The response of rat splenocytes to donor antigen in group 1 and group 3 was obviously lower than that in group 4 (P < 0.01), while the response to unrelated antigen was similar to group 4.CONCLUSION: Donor dendritic cells modified with CTLA- 4Ig can significantly prolonged survival time of rat renal allografts and the administration of both donor and recipient dendritic cells modified with CTLA- 4Ig can induce a longer survival time of renal allografts. Recipient dendritic cells cannot prolong the survival time of renal allografts.

The modernization of Traditional Chinese Medicine (TCM) is urgent need of the modern theory of TCM, is a consensus. But, How to establish and what kind of modern TCM theory, is far from a consensus. Based on the summarization of the common law in the construction of scientific theory, we are trying to give a answer that are expected to form a consensus. Firstly, we have illustrated the trend that the development of TCM theory should learn from the life and medical sciences theory function and value; Secondly, we have proven that TCM and TCM theory belongs to the traditional medicine and theory and pointed out the limitation of the traditional medicine, TCM and its theory. And then we have revealed the inevitable trend of traditional medicine, TCM and its theory development at home and abroad, logically obtained the inevitable choice of constructing the modern TCM basic theory; Lastly, we have analyzed the different discipline new theory construction process and method at home and abroad and discovered the common five steps rule, put forward three principles and five steps of constructing modern TCM basic theory, demonstrated its important role during the theoretical revolution promoting Chinese Medicine theory from phenomenon description to mechanism clarification and the scientific value of Chinese Medicine theory in the domestic and foreign development trend.

Objective To investigate whether reduced or discontinued calcineurin inhibitor (CNI) can improve the renal functions of renal transplant recipients with chronic allograft nephropathy (CAN). Methods A total of 46 renal transplant recipients with declining graft function and biopsy proven CAN were studied. Within 1～2 weeks, CNI (Cyclosporine A or Tacrolimus ) in 27 recipients (group A) was discontinued or reduced to one third of their original doses, but Azathioprine (Aza) or mycophenolate mofetil (MMF) was increased properly. The doses of CNI in the 19 recipients (group B) were not changed obviously, but Aza or MMF was increased properly. At least 1-year follow-up was performed in all patients. Renal functions were compared between the two groups. The incidence of acute renal graft rejection was calculated in both groups. Results One year later, there were 17 patients (63.0%) with stabilized or improved graft function in group A, and 2 (10.5%) in group B. The difference was significant. The incidences of acute rejection in both groups were not significantly different. Conclusion For some renal transplant recipients with declining graft function and biopsy proven CAN, remarkably reduced or discontinued CNI can stabilize or improve their renal functions. Adjusting the doses of immunosuppressive agents does not increase the risk of acute rejection.

Objective To observe the efficacy of local gene transfection in CD154 extracellular domain on the survival of renal allografts. Methods The kidneys of Brown Norway (BN) rats were transfected with CD154 extracellular domain gene recombined adenovirus. The transfected kidneys were transplanted to Lewis rats (transfection group). BN→Lewis kidney transplantation with non transplanted kidneys served as the controls. The allograft survival time and the allograft function between the two groups were compared. Results The allograft survival time of the transfection group was longer than that of the controls significantly [(28?7.3)d vs (8.6?1.2) d, P