Objective:To investigate the promoting effect of nerve regeneration factor(NRF) on nerve regeneration after sciatic nerve injury in rats.Methods: Thirty Sprague-Dawely rats,including 15 females and 15 males,were equally randomized into 3 groups: low dose NRF group,high dose NRF group and control group.Rats' sciatic nerves were injured by crushing and sciatic function index(SFI) was determined by walking tract analysis at days 10,15 and 20 after crushing.Then sciatic nerves on both sides were dissociated for electrophysiology study and the recovery rate of nerve conduction velocity(NCV) was calculated.Then,2 rats were randomly selected from each group and the ultrastructure of regenerated sciatic nerves was observed by electron microscope.Meanwhile,the spinal cord segments(L_4-L_6),the distal site of injured sciatic nerve and the injured gastrocnemius muscle in other rats were observed by light microscope.The count of motor neurons of anterior horn,the number of myelinated fibers,the transverse section area of gastrocnemius muscle cells and other parameters were determined.Results: At day 10 after crushing,SFI scores had no significant difference between 3 groups;at day 15 after crushing,SFI score of high dose NRF group was significantly higher than that of the control group(P

Objective To investigate the knowledge of osteoporosis and its related behavior in middle-aged and elderly people.Methods A total of 1199 residents aged≥45 years in Hangzhou community were investigated by health survey questionnaire of osteoporosis.Results There were statistically significant differences in osteoporosis symptom scores according to ages (aged 45 to 59 years,6.38±2.64; aged 60 to 74 years,5.69±2.83; aged over 75 years,4.43±2.54),and according to education background (middle school or below.4.56± 2.01; secondary school,6.07±2.45; university or higher,8.25±2.69),and according to occupation (mental labour,5.89±3.21;manual labour,4.57 ± 2.36),and residence (city,6.12 ± 3.32 ; countryside,4.34 ± 2.25) (F/t =14.59,26.94,6.29,8.35,respectively,P＜0.01).There were significant differences in types ofexercise between rural and urban population (no exercise or little exercise,30.4％ vs.32.9％; light burden movement,64.4％ vs.53.5％; weight-bearing exercise,5.2％ vs.13.6％; x2 =22.13,P＜0.01).The significant food intake differences were found between rural and urban residents (milk,30.2％ vs.38.3％ sea-food,28.2％ vs.35.7％; bean-products,48.3％ vs.56.1％; fruit,52.6％vs.62.5％; coffee,3.2％ vs.7.4％; x2=8.55,7.77,7.21,19.37,10.14,respectively,all P＜0.01).Conclusions Health education should be individualized based on age,education level and occupation.The elderly and low education groups should be concerned.We should shift the focus of publicity and education to the countryside and enhance the awareness of osteoporosis prevention in order to build a healthy lifestyle.

Objective To study the methods and significance of motor and sensory areas mapping by optical intrinsic signal imaging (IOSI) and neuromonitoring (IOM) during the operation on the lesions near the central sulcus. Methods Intraoperative neuromonitoring were firstly used to map the central sulcus and motor cortex in 5 patients with glioma undergoing surgery. Then, intrinsic optical signals imaging were used to locate the postcentral gyrus. According to the results of mapping, microsurgical operation was chosen for the resection of glioma and postoperative functional results were evaluated. Results All the patients acquired accurate location of central sulcus, motor cortex and somatosensory cortex. The enhanced MRI performed within 24 hours postsurgery showed total resection in 5 cases. The motor and somatosensory function of 5 patients returned to normal after 3-12 months. Conclusion Intraoperative location of the somatosensory area and motor area by optical intrinsic signal imaging and neuromonitoring is a reliable and safe method.

In order to improve the reliability of cardiac pacemaker contact-less power supply technology, this paper proposes a novel application of wireless feedback voltage stabilizing technology to adjust heart disease patients with inner power supply filter circuit output voltage and current control method, to keep the output voltage stability, and to ensure that the super capacitor and cardiac pacemaker to get a stable power supply. To implement the real-time accurate voltage control with considering the primary and secondary side inductance coupling coefficient changes, the change of the external power supply voltage and load, it is necessary to test thee real-time and accurate output voltage and current value after rectifying filtering. Therefore, based on the chaotic control theory, we adopted method of phase diagram on the basis of the quick observation after rectifying filtering, so that the method of voltage and current could improve the detection time of the circuit. The phase diagram of proposed control method can be divided into 8 segments, and we got 7 zero-extreme points. When these zero-extreme points are detected, according to extreme points of the zero instantaneous values, the corresponding average values of voltage and current were obtained. Simulation and experimental results showed that using the above method can shorten the response time to less than switch devices 1/2 switching cycles, thus validating the effectiveness and feasibility of the proposed detection algorithm.
Algorithms ; Electric Power Supplies ; Feedback ; Humans ; Nonlinear Dynamics ; Pacemaker, Artificial ; Wireless Technology

0.05),but improved significantly the rise from day 3 to day 10 of serum fibronectin level 〔(22.8?5.8) mg/L vs.(9.6?3.6) mg/L,P

Objective To explore the effect of multipore-light brown fixation on preventing peripherally inserted central catheter from prolapsing. Methods Randomizedly 82 patients with PICC were equally divided into treatment group and control group. Multipore-Light Brown fixation was used in the treatment group to stabilize catheter , while the routine care was applied in the control group for stabilization . The two groups were compared in terms of prolapse rate and degree of the PICC . Result The prolapse rate and the prolapse degree of the treatment group were significantly lower than that of the control group ( P < 0 . 01 ) . Conclusions The multipore-light brown fixation is more effective in the stabilization of PICC. It can effectively prevent the PICC from prolapsing.

The primary neural stem cells were isolated from SD rat and formed the neuropheres, the neuropheres were passaged and planted on the dish coated with 0.1% gelatin, the colony was picked up under the microscope, then dispersed and cultured, to obtain the clone proliferated from one cell, passaging and picking up the cells 5～6 times at least. The NSC and its differentiated cells were identified with the marker genes respectively. The results showed that the neural stem cells were isolated from the SD rat embryos and the real clone were obtained by picking up the cells again and again, and then cultured in the form of monolayer. The marker genes of the neural stem cells and its differentiated cells could be detected at last. It will provide the rat model the resource of the cells for the treatment and the basic research for the morphology standard.

Objective To investigate the status of knowledge,attitude and behavior of schistosomiasis control of rural resi-dents in Wanjiang River region after a flood,so as to provide the reference for targeted health education. Methods The multi-stage sampling was applied to select the respondents in rural residents in Wanjiang River region,and the self-designed question-naire was used to investigate the current situation of knowledge,attitude and behavior of schistosomiasis prevention and control of the rural residents. Results The total awareness rate of knowledge about the prevention and control of schistosomiasis was 47.92%. The age,education,family income,relatives and friends with medical background,and health education significantly influenced the awareness rate(χ2=12.76,89.19,18.19,50.83 and 92.60 respectively,all P<0.05). The accuracy rates of at-titude and behavior in schistosomiasis control were 62.89%and 52.37%respectively. Conclusion The awareness rate of knowl-edge about the prevention and control of schistosomiasis,and the accuracy rates of attitude and behavior in schistosomiasis con-trol of the rural residents in Wanjiang River region are all inefficient,and therefore,the targeted health education should be strengthened to decrease the risk of schistosomiasis transmission.

Objective To investigate the correlation between hypoglycemia and brain injury of newborns.Methods Medical records and follow-up data of 110 newborns with hypoglycemia (blood glucose level≤2.2 mmol/L) who admitted into neonatal department of Peking University First Hospital from December 2006 to December 2009 were studied.All patients were divided into 3 groups:no brain injury group,mild and severe brain injury group according to their clinical manifestation,cerebral radiological characteristics and cerebral functional tests.By using receiver operating characteristic (ROC) curve and x2 test,the potential optimal blood glucose level and duration of hypoglycemia for predicting brain injury were confirmed.Multivariate Logistic regression was taken to determine independent predictors for brain injury.The analyzed factors included gender,preterm/small for gestational age,hyperbillirubinemia,fetal distress,asphyxia,infection,seizures and maternal hypertensive disorder complicating pregnancy and hyperglycemia.Results Among the 110 hypoglycemia newborns,33 (30.0％) infants suffered from brain injury,of which 23 were mild and 10 were severe.Blood glucose ≤1.7 mmol/L had high specificity (73％) and sensitivity (60％)for predicting brain injury.When blood glucose≤ 1.7 mmol/L,the incidence of brain injury and severe brain injury was 43.6％ (24/55) and 18.2％ (10/55),which was higher than those [16.4％(9/55) and 0.0％ (0/55)] of patients whose glucose level ＞1.7 mmol/L(x2 =9.74 and 11.00,P＜0.01 respectively).Blood glucose ≤ 1.2 mmol/L had high specificity (100％) and sensitivity (81％) for predicting severe brain injury.When blood glucose ≤1.2 mmol/L,the incidence of severe brain injury was higher than that of the patients whose glucose level was higher than 1.2 mmol/L [34.5％ (10/29) vs 0.0％ (0/81),x2 =30.72,P＜0.01].Duration of hypoglycemia ≥12 h had specificity (100％) and sensitivity (36 ％) for predicting brain injury.When duration of hypoglycemia ＜12 h,the incidence of brain injury was lower than that of the patients whose duration of hypoglycemia≥12 h [21.4％ (21/98) vs 6/6,x2 =27.69,P＜0.01].Multivariate Logistic regression showed that fetal distress (OR=4.69,95％CI:1.47-14.97,P=0.009),glucose level≤1.2 mmol/L (OR =5.16,95％CI:1.56-17.03,P=0.007),duration of hypoglycemia≥12 h (OR=8 885 220 297.12,95％CI:0.00-∞,P =0.000) and maternal hyperglycemia (OR =3.34,95％CI:1.01-11.02,P=0.048) were independent risk factors for neonatal brain injury.Conclusions Low blood glucose level and prolonged hypoglycemia might induce injury of neurol system.Fetal distress and maternal hyperglycemia might increase the incidence of brain injury in newborns with hypoglycemia.

The following problems in medical journals of China were analyzed, including limited free full-text access, low international influence power, severe duplicated construction, academic misconducts, weakened peer review, and overemphasis on journal assessment indexes, with suggestions put forwards for their solution, such as exploring novel channels for digital publication and distribution , actively preparing journals publishedin English , and improving the academic level of journals.