ObjectiveTo investigate the relationship between post-stroke depression (PSD) and mood of anxiety and cognitive function, and to explore prognostic condition.Methods144 post-stroke patients were examined with the Self-rating Depression Scale (SDS), the Self-rating Anxiety Scale (SAS) and Scale of Elderly Cognitive Function (SECF). Scores of the depression group were SDS≥50 and scores of the group without depression were SDS<50. ResultsThe incidence of the depression was 40.27%, the suicidal intention was 17.36%. There was significant difference between two groups with lower cognitive function and higher rates of anxiety in the depression group (P<0.001). The re-examination revealed the emotion of depression and anxiety reduced obviously in the depression group after 2 months (P<0.001), the emotion of anxiety also reduced in the group without depression (P<0.01). The cognitive function had little improvement in two groups.ConclusionsPSD is closely related to the emotions of anxiety and cognitive function of patients, emotions of depression and anxiety all improve after 2 months hospitalization, emotions of anxiety also reduce in the group without depression. But the cognitive function improves slower, with no evident changes in 2 months.

ObjectiveTo study the relationship among positive, negative trait coping style and possible factors in college students of rehabilitation specialty.Methods38 college students of rehabilitation specialty were assessed with Trait Coping Style Questionnaire (TCSQ), Coping Style Questionnaire (CSQ), Eysenck Personality Questionnaire (EPQ), Symptom Checklist 90 (SCL-90), Self-rating Depression Scale (SDS), and Self-rating Anxiety Scale (SAS). Gradual regression analysis was applied, in which, sub-scales of positive coping and negative coping of Trait Coping Style Scale were taken as dependent variable, while other scales were taken as independent variable.ResultsPositive coping had significantly correlated positively with EPQE and factor of asking for help, and negatively with EPQN and factors of elusion and sensitivity of human being. Negative coping significantly correlated positively with SDS, EPQN, factors of self-blame, hostility and psychosis, but negatively with factor of solving problem.ConclusionPhysical and mental health can be kept by maintaining emotion stabilization, reducing negative coping of self-abasement, self-blame, hostility and elusion, using positive coping of solving problem and asking for help.

ObjectiveTo explore the mental health status and related factors of college students of rehabilitation medicine.Methods38 college students of rehabilitation medicine were assessed with Symptom Check List 90 (SCL-90), Eysenck Personality Questionnaire (EPQ), Coping Style Questionnaire (CSQ), and Adolescent Self-rating Life Events Checklist (ASLEC). Data of students of rehabilitation medicine, medical students, common university students and young people were compared.ResultsSCL-90 scores of students rehabilitation medicine were significantly lower than that of medical students and common university students, also lower than that of young people (besides somatization and anxiety factors), and scores of some sub-scales of EPQ, CSQ and ASLEC of students of rehabilitation medicine were positive or negative relationship with some SCL-90 factors in different degrees (P<0.05～0.01).ConclusionThe mental health status of students of rehabilitation medicine is good in general, and the main affected factors on mental health are instability emotion, negative daily events and immature coping style.

ObjectiveTo explore the relationship between cognitive impairment after stroke and possible factors.MethodsThe stroke patients being in hospital from 1977 to 2004 were evaluated with (scale of elderly cognitive function, SECF), the self-rating depression scale (SDS), the self-rating anxiety scale (SAS) and the ability of daily living (ADL). 236 cases with SECF≤90 were considered as cognitive impairment and retested with 4 scales mentioned above 2 months later. Gradual regression analysis was applied, one factor was taken as dependent variable selected from sex, age, education, course of disease, etiological factor, hemiplegia side, SECF1, SECF3, ADL1, ADL3, SAS1, SAS3, SDS1, SDS3, while others were taken as independent variable.ResultsAfter two months treatment in hospital, scores of SECF and ADL of patients improved, SAS and SDS reduced significantly (P=0.000). The proportion of female and cerebral infarction in aged patients was higher and scores of ADL reduced significantly (P<0.05). The SDS scores of patients with long course of disease before hospitalization were higher; there was a small improved extent for ADL ( P<0.05). SAS scores of patients with high ADL were low (P=0.028).ConclusionEarly residential rehabilitation can improve ADL function and reduce depression of stroke patients.

ObjectiveTo explore the relationship between depression in stroke patients with cognitive impairment and possible factors. Methods236 cognitive impaired patients whose score of Scale of Elderly Cognitive Function (SECF) ≤90 were selected in this study from inpatients with stroke from 1997 to 2004. They were evaluated with SECF, the Self-rating Depression Scale (SDS), the Self-rating Anxiety Scale (SAS) and the Ability of Daily Living (ADL) immediately and 2 months after hospitalization.72 patients were in the depression group(SDS≥50),and 164 patients were in the no-depression group(SDS<50). Gradual regression analysis was applied. One factor was taken as dependent variable selected from immediate scores of SECF, ADL, SAS, and the change of scores of SECF, ADL, SAS, SDS, respectively, while others were taken as independent variables. ResultsThe improvement of depression and ADL were helpful to reduce the anxiety and improve cognitive function in depression group (P<0.05), as well as the improvement of anxiety and cognitive function were helpful to improve the depression and ADL (P<0.05).The negative correlation were found between the difference former and latter score of SDS and ADL, as well as between those of SAS and SECF (P<0.05).ConclusionPatients with cognitive impairment can be beneficial from reducing depression and anxiety companied with improving the ADL and cognitive function, which is also mutually beneficial.

Breast cancer stem cells comprise a sub-population, which enables the capacity for self-renewal and the potentiality for differentiation and high tumorigenicity. Growing evidence suggests that breast cancer stem cells most likely contribute to tumor generation, progression, relapse, metastasis and therapeutic resistance. Herein, the recent advances in breast cancer stem cells were highlighted in this review.

156 stroke patients were assessed retrospectively by Self Depression Scale. The incidence of depression after stroke,and the relationship of depression between age, course, location of impairment, the character of the stroke,and ADL ability were included in the study. The conclusion showed that depression was related significantly to severe impairment in ADL.

ObjectiveTo investigate the effect of puerarin on the expression of pigment epithelium-derived factor (PEDF) in the retina of diabetic rats.MethodsThe diabetic retinopathy rat model was established.Rats were randomly divided into three groups (normal,diabetic,and diabetic with puerarin injection).The pathological changes of the retinopathy were examined.PEDF was analyzed with PCR in different time point.Results Body weight of diabetic group [ ( 216.9 ± 8.37 ) g,and(179.1 ±7.56)g] was compared with normal group[ (298.2 -±6.78)g,and(323.5 -±6.45)g] in the 3 month and 5 month ( q =12.39,P ＜0.01 ; q =15.47,P ＜0.01 ).Blood glucose of diabetic group and diabetic with puerarin injection group was compared with normal group at each time point ( P ＜ 0.01,P ＜ 0.05 ).HE staining of the retina of diabetic rats indicated a diabetic retinopathy occurred in the early - stage in diabetic group,but changed slightly in the diabetic model with an injection of puerarin.The PEDF expression at the 5 month time point in normal group,diabetic group,and diabetic group with puerarin injection was 8.833 (1.200),1.650(3.300),and 8.350( 1.100),respectively.Compared with normal group and diabetic group with puerarin injection,the level of PEDF was significantly decreased in diabetic group (x2 =48.57,P ＜ 0.01 ;x2=48.46,P ＜ 0.01 ).ConclusionsPuerarin can alleviate the retinal pathological damage of diabetic rats,early intervention with puerarin can prevent from the expression of PEDF and delay the diabetic retinopathy process.

The microstructure of cationic cyclopeptide (TD-34) treated Caco-2 cell membrane was observed, and we discussed the relationship between membrane structure and insulin transmembrane permeability. Atomic force microscope (AFM) was used to observe living cell membrane in air condition and tapping mode. Results showed that the surface of Caco-2 cell membrane treated with TD-34 lost its smoothness and nearly doubled its roughness. Apparent permeability coefficients (P(app)) of insulin in Caco-2 cell monolayers increased 2.5 times. In conclusion, AFM can be used to observe microstructure of cationic cyclopeptide treated cell membrane and cationic cyclopeptide enhanced insulin delivery across Caco-2 cell membrane by increasing membrane fluidity.

Objective To explore the therapeutic effects and the mechanisms of Faecalibacterium prausnitzii (Fp) on trinitro-benzene-sulfonic acid-induced colitis.Methods Sixty Sprague-Dawley rats were divided into healthy control group, colitis model control group, Fp pretreated group,Fp supernatant pretreated group,Fp treated group and Fp supernatant treated group.Disease activity index (DAI),histological injury of colonic tissue,the content of butyrate in feces,forkhead box protein 3 (Foxp3) regulatory T cells (Treg) in peripheral blood and spleen and the level of interlenkin (IL)-17 and IL-6 in serum were evaluated.All the data were statistical analyzed by single factor analysis of variance. Results Compared with colitis model control group, DAI significantly lowered and histological injury obviously improved in Fp pretreated group, Fp supernatant pretreated group,Fp treated group and Fp supernatant treated group.The effects of Fp pretreated group were better than those of Fp treated group and Fp supernatant pretreated group were better than Fp supernatant treated group.The concentration of butyrate in Fp pretreated group,Fp supernatant pretreated group,Fp treated group and Fp supernatant treated group was (3091.08 ±485.50) × 106 mol/L,(1714.64 ± 351.25) × 10(-8) mol/L,(2064.75 ± 295.04) × 10-6 mol/L and (1089.13±321.23) × 10-6 mol/L respectively,there was significant difference between Fp pretreated group and other groups (F=49.796,P＜0.01).The peripheral blood level of Foxp3+ Treg in Fp supernatant pretreated group was highest.The spleen level of Foxp3+ Treg in Fp pretreated group and Fp supernatant pretreated group were significantly higher than that of other groups.The serum level of IL-17 and IL-6 in Fp pretreated group,Fp supernatant pretreated group,Fp treated group and Fp supernatant treated group was significantly lower than that of colitis model group.Conclustons Fp plays a role in promoting the repair of intestinal inflammatory reaction in colitis model rats.The mechanism may be related with butyrate producing,the peripheral blood and spleen level of Foxp3+ Treg up-regulating,suppressing the secretion of proinflammatory cytokine IL-17 and IL-6.Rebuilding the balance of Treg/Th17 to reduce local intestinal inflammation.