ObjectiveTo investigate the effect of Buchangwenxin particles in maximum P wave (Pmax), dispersion of P wave(Pd) of elder patients with diastolic cardiac dysfunction and paroxysmal atrial fibrillation(PAF).Methods115 elder patients with diastolic cardiac dysfunction and PAF were randomly divided into two groups:group A received the routine drug therapy,and group B received Buchangwenbxin particles with routine drug therapy.Another 54 elder patients with diastolic cardiac function but without PAF was selected as control group.Changes in Pmax and Pd were compared between groups with or without PAF as well as subgroups.ResultsPmax and Pd were wider in PAF group than no PAF group(all P ＜0.01).There was no difference between group A and B in Pmax and Pd (all P＞ 0.05), and there were less atrial fibrillation as well as shortened Pmax and Pd in group B, which was significantly different with group A(all P ＜0.01).ConclusionPmax and Pd were wider in elder patients with diastolic cardiac dysfunction and PAF than those without PAF,and Buchangwenxin particles could shorten Pmax and Pd and reduce the attacks of atrial fibrillation.

Objective To evaluate the early diagnostic value of cerebrospinal fluids (CSF) procalcitonin(PCT) for the post-neurosurgery bacterial meningitis (PNBM).Methods Conduct a prospective study in 34 patients who suspected PNBM in ICU of Kowloon Hospital of Suzhou.On the first day, all the patient blood samples were obtained for detection of routine blood count, C-reactive protein, PCT.Also, all the patient CSF samples were obtained for detection of routine CSF count, biochemical tests, PCT and for CSF culture.The patients were divided into PNBM group and non-PNBM group according to PNBM diagnostic criteria.Results Fifteen cases(44.12％) patients were diagnosed as PNBM,the other 19 cases were non infection group.Levels of CSF procalcitonin PCT 0.50 μg/L were significantly higher in patients with PNBM than those non-PNBM 0.29 μg/L on the first day(P＜0.01).The ROC curues indicated that the area under the curve (AUC) for CSF PCT was 0.846, it was significantly higher than the other traditional indicators' area.The cut off points of CSF procalcitonin was set to be 0.415 μg/L for patients with proven PNBM.The corresponding sensitivity, sepecificity were 80.0％ and 73.7％.Conclusion The level of the CSF procalcitonin may be valuable early diagnostic parameter for PNBM.Sensitivity and specificity of the CSF procalcitonin was higher than the other traditional indicators.

β-arrestins are involved in the insulin signaling pathway, affecting peripheral insulin resistance,also affecting insulin secretion and lipid metabolism. To clarify β-arrestins mediated signaling pathways is an approach to the pathogenesis and effective treatment of diabetes.

Objective To explore the clinical significance of dynamic determination of serum procalcitonin(PCT)levels in judgment of infection and prognosis in patients with severe craniocerebral injury.Methods A total of 80 patients with severe craniocerebral injury were selected and they were divided into infection group and non-infection group.Infection group was divided into improved group and non-improved group.Acute physiology and chronic health evaluation Ⅱ(APACHE Ⅱ)score and Glasgow coma scale(GCS)score was compared between infection group and non-infection group.The level of serum PCT,white blood cell count(WBC),C-reactive protein(CRP)and interleukin(IL)-6 was compared on the first,third,fifth day of admission in intensive care unit among these groups.Results Infection group in 48 patients,and non-infection group in 32 patients.There was no significant difference in age,sex and GCS score between two groups(P ＞0.05).APACHE Ⅱ score in infection group was higher than that in non-infection group [(21.71 ± 7.13)scores vs.(15.32 ± 6.17)scores],and there was significant difference (P＜ 0.05).The level of serum PCT on the first day of admission in intensive care unit in infection group was higher than that in non-infection group[(2.15 ± 1.79)ng/L vs.(0.56 ± 0.47)ng/L],and there was significant difference(P＜ 0.05).There was no significant difference in WBC,CRP and IL-6 between two groups(P＞0.05).The level of serum PCT,WBC,CRP,IL-6 on the third day of admission in intensive care unit in infection group was higher than that in non-infection group,and there was significant difference(P＜ 0.05).The level of serum PCT on the fifth day of admission in intensive care unit in infection group was higher than that in non-infection group [(3.09 ± 1.98)ng/L vs.(1.06 ± 0.63)ng/L],and there was significant difference (P＜0.05).WBC,CRP,IL-6 on the fifth day of admission in intensive care unit was(15.13 ±6.32)×109/L,(103.56 ±40.62)mg/L,(230.41 ± 121.83)ng/L in improved group(38 cases),and (18.62 ±8.89)× 109/L,(134.38 ± 34.16)mg/L,(268.73 ± 138.12)ng/L in non-improved group(10 cases),and there were significant differences between two groups(P ＜ 0.05).The level of serum PCT on the first,third,fifth day of admission in intensive care unit in improved group was lower than that in non-improved group,and there was significant difference(P ＜ 0.05).Conclusion The alteration of serum PCT level may be used as one of the effective predictors for infection in patients with severe craniocerebral injury and for their prognosis.

Objective To study the expression of cyclooxygenase-2 (COX-2) in the gastric epithelial carcinogensis and its relation with cell apoptosis and proliferation. Methods 15 cases of normal gastric mucosa (NGM), 30 cases of intestinal metaplasia (IM), 30 cases of dysplasia (Dys) and 40 cases of gastric cancer (GC) were used in this study. The expressions of COX-2 and proliferating cell nuclear antigen (PCNA) were detected by immunohistochemical SABC method, and cell apoptosis was measured by TUNEL. Results The expression level of COX-2 gradually increased from NGM, IM, Dys to GC. The positive rate of COX-2 in GC was 67.5%, 13.3% in NGM, and 33.3% in IM. There was a significant difference in COX-2 expression between GC and IM, GC and NGM(P0.05). The expression of COX-2 in poorly differentiated cancer was markedly higher than that in well differentiated cancer (P

BACKGROUND:Col agen-gelatin composite scaffolds have been reported to be able to promote the early recovery of peripheral nerve defects. However, this conclusion has not been further confirmed. OBJECTIVE:To investigate the biocompatibility of the col agen-gelatin scaffold and its treatment outcomes in the repair of peripheral nerve defects. METHODS:The col agen-gelatin scaffold was co-cultured with bone marrow mesenchymal stem cel s (BMSCs) of Sprague-Dawley rats for 5 days, and then the cel growth was observed. Twenty Sprague-Dawley rats were enrol ed, modeled into a left 30-mm peroneal nerve defect and randomized into experimental and control groups. The col agen-gelatin scaffold composited with BMSCs was implanted into the experimental group, and autograft nerve implanted into the control group. Morphology of the middle bridge was observed, and electrophysiology was conducted at 16 weeks after implantation. RESULTS AND CONCLUSION:BMSCs grew and adhered wel onto the scaffold. The myelinated nerve fiber density did not significantly differ between groups (P>0.05). The myelinated nerve fiber diameter, myelin sheaththickness and percentage of nerve tissues in the experimental group were significantly lower than those in the control group (P<0.05). There were no significant differences in the conduction velocity, latency of motor nerves and the conduction velocity and amplitude of sensory nerves between groups (P>0.05). The amplitude of motor nerves and the latency of sensory nerves in the experimental group were significantly lower than those in the control group (P<0.05). To conclude, the col agen-gelatin scaffold holds a good cytocompatibity and is ideal for the repair of peripheral nerve defects.

Objective To explore the clinical outcome of individuals with Clinical High Risk (CHR) for psychosis and its relationship with Theory of Mind (ToM) function.Methods The Structured Interview for Prodromal Symptoms/Scale of Prodromal Syndromes (SIPS/SOPS) was applied to assess prodromal psychosis.The Reading the mind in the Eyes and faux pas Task were conducted to assess the function of Theory of Mind among the individuals of clinical high risk of psychosis.All participants had completed the 2-year follow-up.Conversion was determined using the criteria of presence of psychotic symptoms (POPS).According to the outcome,CHR individuals were divided into conversion group (n=20) and no-conversion group (n=50).The baseline clinical symptom characteristics and Theory of Mind were compared between groups.Results There was no significant difference in clinical symptom characteristics among individuals with CHR (P＞0.05).In the faux pas text,there were significant differences in Faux Pas Detection (P=0.01),Faux Pas Understanding (P=0.01) and Faux Pas Total (P=0.02) but not in control stories and the Reading the Mind in the Eyes Test between convertors and non-convertors (P＞0.05).Conclusion The ToM disability in clinical high risk population increases risk for conversion to psychosis.

Objective To compare and analyze the detection situation of common pathogens in child respiratory tract infection in our hospital and to provide the basis for clinical accurate diagnosis and reasonable treatment .Methods A total of 6 032 specimens were collected from the children patients with acute respiratory tract infection in the Gansu Provincial Maternity and Child Health Care Hospital from January to December 2015 .The immunodot test technology was adopted to detect the 6 kinds of early specific serum antibody IgM of common respiratory pathogens :adenovirus (ADV ) ,influenza virus (IV ) ,parainfluenza (PIV ) ,respiratory syncytial virus (RSV) ,mycoplasma pneumoniae (MP) and chlamydia pneumonia (CP) .The differences in children infection pat‐terns ,different ages ,seasons and genders were analyzed .Results Among 6 032 specimens ,2 279 cases were positive with the posi‐tive rate of 37 .8% .The positive rates of 6 kinds of respiratory pathogens were in turn 12 .2% for ADV ,9 .6% for IV ,7 .9% for CP ,4 .6% for MP ,3 .0% for RSV and 0 .3% for PIV .The detection rate of respiratory tract pathogens was higher in spring and summer ;age groups of 1-3 years old and 4-6 years old had higher positive detection rate .Conclusion The main common patho‐gen of respiratory tract infection in this hospital is ADV ,followed by IV .Their infection has obvious seasonality .The mycoplasma and chlamydia also are the common pathogens of respiratory tract infection ,which are dominated by MP and CP .

OBJECTIVE:To compare home-made mu'an-eye-gel(acyclovir plus honey) with commercial aciclovir(ACV)-eye-gel in releasing drug characteristics in vitro.METHODS:The in vitro drug release test was conducted by the third method of dissolution determination stated in Chinese Pharmacopeia together with bag filler method.The cumulative drug-releasing percentage and the acyclovir amount in mu'an-eye-gel versus ACV-eye-gel were determined by UV spec-trophotometry,and the accumulative releasing drug percentages of the two preparations were computed and their drug release behaviors w ere compared.RESULTS:The in vitro releasing behaviors of mu'an-eye-gel followed the Weibull kinetic equa-tion,however the vitro releasing behavior of commercial ACV-eye-gel followed the zero order kinetic equation,and the T80%and Q8 h had statistical significances between(mu'an-eye-gel:T80%=3.156?0.013(h),Q8 h=93.28?0.010(%);ACV-eye-gel:T80%=10.16?0.009(h),Q8 h=67.85?0.025(%)) 2 kinds of preparation.CONCLUSION:Mu'an-eye-gel is superior to the commercial ACV ophthalmic gel in both releasing velocity and accumulative drug release percentage.

Objective To explore the risk factors affected the timing of endoscopic examination in nonvariceal upper gastrointestinal bleeding (NVUGIB).Methods From January 2001 to December 2011 , 441 patients diagnosed as NVUGIB under endoscope were enrolled,and they were divided into the group receiving endoscopy within 24 hours (233 cases)and the group receiving endoscopy beyond 24 hours (208 cases).The demographic and clinical characteristics of the two groups were analyzed.Student′s t test was used for measurement data comparison and chi-square test for numerical data.Logistic regression analysis was used to analyze the multivariate factors which influenced the timing of endoscopy examination.Results The rates of complication in the group receiving endoscopy within 24 hours and the group receiving endoscopy beyond 24 hours were 9.0% (21/233)and 19.7% (41/208),and the difference was statistically significant (χ2 =10.411 ,P =0.001 ).The mean systolic blood pressure were (118 ±19 )and (122±19)mmHg (1 mmHg=0.133 kPa),respectively,and the difference was statistically significant (t=2.099,P = 0.036 ).On admission the mean hemoglobin were (98 ± 27 )and (93 ± 28 )g/L, respectively,and the difference was not statistically significant (t=1 .640,P =0.102).The mortality rate were 1 .3% (3/233 )and 5 .3% (11/208 ),respectively,and the difference was statistically significant (χ2 =5 .723,P =0.017).With multivariate Logistic regression,multivariate factors analysis indicated that complication was the main factor influenced the timing of endoscopy examination (OR =2.710,95 %CI :1 .502 to 4.899,P < 0.01).However,age,gender,systolic blood pressure,hemoglobin levels on admission,location of lesion and clinical symptoms were not related with timing of endoscopy examination (all P >0.05).Conclusion Complication is a risk factor in the timing delayed endoscopy.