1.Two cases of shiitake dermatitis
Wei SHI ; Jingwei ZHANG ; Hongfu XIE ; Xiang CHEN ; Mingliang CHEN
Chinese Journal of Dermatology 2014;47(3):208-209
Two patients who developed typical skin eruptions 24 hours after consumption of shiitake mushrooms are reported.Case 1:a 56-year-old woman suddenly developed widespread itching eruptions one day after intake of shiitake mushrooms.On examination,there were erythematous and edematous linear streaks (flagellate erythema) over the neck,trunk and limbs.Case 2:a 60-year-old man presented with edematous flagellate erythema over the trunk and limbs for four days.He reported intake of shiitake mushroom several days prior to the presentation.Pathological examination revealed focal parakeratosis,intracellular and intercellular edema in the prickle cell layer,severe edema of papillary dermis,evident widening of interfibrous spaces,dilation and congestion of capillaries in the superficial dermis with a mixed perivascular infiltrate of massive lymphocytes and sparse neutrophils.Both patients were diagnosed with shiitake dermatitis,and treated with prednisone and antihistamines.The lesions subsided after 3 and 4 days of treatment in patient 1 and 2 respectively.
2.Cellular Distribution of a-catenin in Epidermal Tumors
Xiang CHEN ; Hongfu XIE ; Qianjun DU ; Mingliang CHEN ; Fuwen CHEN ;
Chinese Journal of Dermatology 1994;0(06):-
Objective To examine the cellular distribution and the role of a-catenin in epidermal tumors. Methods Expression of a-catenin was investigate d by immunohistochemical staining in 20 patients with Bowen′s disease (BD), 20 squamous cell carcinoma (SCC), 20 basal cell carcinoma (BCC), and 40 normal cont rols. Results Expression of a-catenin was strongest on the cell membranes of b asal cells, but nearly negative in the cytoplasm and the nuclei of epidermal cel ls in normal controls. Expression of a-catenin was significantly lower on the cell membranes in SCC and BCC cells than that in normal epidermal cells (P
3.Effect of UVA irradiation on proliferation and NO/iNOS system of human skin fibroblast
Mingliang CHEN ; Guiying ZHANG ; Mei YI ; Xiao CHEN ; Ji LI ; Hongfu XIE ; Xiang CHEN
Journal of Central South University(Medical Sciences) 2009;34(8):705-711
dosage ( P<0.01).Conclusion UVA can inhibit the proliferation activity of human skin fibroblasts. It might be related to the up-regulation of iNOS gene expression and the over-secretion of NO induced by UVA.
4.Effect of triptolide on the proliferation and apoptosis of human epidermal squamous cell carcinoma cell line A431 in vitro
Mingliang CHEN ; Shuai TAN ; Guiying ZHANG ; Mei YI ; Dan JIAN ; Hongfu XIE ; Xiang CHEN
Journal of Central South University(Medical Sciences) 2009;34(7):638-641
Objective To investigate the effect of triptolide on the proliferation and apoptosis of human epidermal squamous cell carcinoma cell line A431 in vitro. Methods Human epidermal squamous cell carcinoma cell line A431 was cultured. After the treatment with triptolide, the inhibi-tion of cellular growth was determined by measuring MTT dye absorption of the living cells. Light mi-croscope showed morphological changes. The cell cycle and apoptosis rate were assessed by flow cy-tometry. Results Triptolide could significantly inhibit the proliferation of A431 cells in a dose- and time-dependent manner. Triptolide could also cause cell morphological changes ( the number of float-ing cells and nuclear pyknosis increase), induce cell apoptosis, and change the distribution of cell cycle phase in A431 cells. Compared with the control group, the G0/G1 phase A431 cell rate in-creased and the rate of S phase cell decreased in TP-treated group. Cell cycles were obviously inhibi-ted by triptolide in G0/G1 phase (both P<0.05). Conclusion TP could play an anti-tumor role by effectively inducing cell apoptosis and inhibiting the proliferation of A431 cells.
5.Study on the expression of high mobility group box chromosomal protein 1 in skin lesions in patients with lupus erythematosus
Jie LI ; Hongfu XIE ; Xiang CHEN ; Mingliang CHEN ; Jianglin ZHANG ; Jinmao LI
Chinese Journal of Rheumatology 2008;12(11):771-774,封3
Objective To study the expression of high mobility group box chromosomal protein 1(HMGB-1) in the skin lesions of patients with lupus erythematosus and investigate the role of HMGB-1 in the pathogenesis of lupus erythematosus. Methods Immunohistochemical assay and Western-blot were used to test the expression of HMGB-1 in skin lesions from 20 discoid lupus erythematosus (DLE) patients, 25 systemic lupus erythematosus (SLE) patients and 20 healthy controls. Results In healthy controls, H MGB-1 was mainly expressed in the nucleus of keratinocytes. In skin lesions of DLE patients, HMGB-1 was mainly expressed in mononuclear cells of dermis, but the percentage of positive keratinocytes in epidermis of lesion area was decreased than that of healthy controls (t=11.315, P<0.01). In skins that were not involved,HMGB-1 was also expressed in the nucleus of keratinocytes and there was no difference in the percentage of positive keratinocytes between DLE and healthy controls (P>0.05). By Western-blot, there was no stati-stical significance in total protein between DLE and healthy controls (t=0.681, P>0.05). In SLE, besides the mononuclear cells, HMGB-1 could be detected both in the cytoplasmic and extracellular space in dermis,while the HMGB-1 nuclear expressions in keratinocytes'of epidermis were decreased than those of DLE (t=6.821, P<0.01), and in un-involved skin, HMGB-1 was also expressed in the nucleus of keratinocytes and there was no difference in the percentage of positive keratinocytes with healthy controls (P>0.05). The total protein was increased in SLE than that of healthy controls and DLE patients (t=15.494, P<0.01 ; t=13.221, P<0.01, respectively). The intensity of HMGB-1 was con'elated with SLEDAI and proteinuria (r=0.565, P<0.01,OR=1.027, P<0.05, respectively). Conclusion Compared with healthy controls, there is translocation and alteration of HMGB-1 expression in patients with lupus erythematosus, which indicates that HMGB-1 may be involved in the inflammation of lupus erythematosus.
6.Expression of inducible nitric oxide synthase and level of nitric oxide in HaCaT cells after UVA irradiation
Ji LI ; Wei LI ; Hongfu XIE ; Xiao CHEN ; Xiang CHEN ; Mingliang CHEN
Chinese Journal of Dermatology 2009;42(6):396-398
Objective To investigate the effects of UVA irradiation on the expression of inducible nitric oxide sgnthase(iNOS)and level of nitric oxide(NO)in HaCaT cells.Methods HaCaT cells were cultured,irradiated with difrerent doses(1,5,1 0 J/cm2)of UVA.After another 24-,48-,72-hour culture,the mRNA and protein expressions of iNOS and level of NO wen measured bv RT-PCR,Westem blot and Griess reagent kit,respectively.Results Atier the irradiation with 1.5 or 10 J/cm2 of UVA,the mRNA expression of iNOS was observed in HaCaT cells at 24 h,reached a peak at 48 h,then decreased at 72 h;significant differences were fcIund between the three time points (all P<0.05).No expression of iNOS protein was detected in HaCaT cells at any time point after irradiation witll UVA Of 1 J/cm2;meanwhile,with UVA irradiation of 5 and 10 J/cm2,the protein expression of iNOS increased at 24 h,peaked at 48 h,but was undetected at 72 h.In HaCaT cells irradiated with UVA of 1.5 or 10 J/cm2.the level Of NO showed an increase at 24 h.a marked increase at 48 h,and a stable increase at 72 h,and significant differences were noticed between irradiated cells and control cells at three time points(all P<0.05).In unirradiated HaCaT cells,no expression of iNOS mRNA or protein was observed with a low level of NO.Conclusions The changes in iNOS expression and NO level in HaCaT cells are related to the duration and dose of UVA irradiation.
7.Comparisons of clinical and laboratory features among patients with measles in different age groups
Jie LI ; Hongfu XIE ; Xiang CHEN ; Yongjun TANG ; Yi OUYANG ; Mingliang CHEN
Chinese Journal of Dermatology 2008;41(4):230-233
Objective To compare the clinical features between measles in adult and juvenile patients,and to provide references for correct diagnosis and early prevention of measles.MethodsClinical data werle retrospectively collected from and analyzed for 239 inpatients with measles in the hospital.Results The ratio ofmales to females was 1∶1.4 and 2.9∶1 among adult patients(n=129)and juvenile patients (n=110),respectively.The average duration from fever to eruption was shorter in adults than in juveniles (2.43±1.34)days vs(4.26±2.69)days,(t=6.48,P<0.01).The incidence of hyperthermia,catarrhal symptoms,Koplik spots,conjunctival hyperemia,diarrhea and thrombocytopenia was 33.0%,65.0%,78.3%,84.5%,42.7%and 17.8%,respectively aniong adult patients,14.5%,50.9%,56.4%,69.0%,22.9%and 9.0%,respectively among juveniles;the difference was significant between the two groups in all the above rates (P<0.01 or 0.05).Meanwhile,elevated activity of lactate dehydrogenase(LDH),creatine kinase(CK),MB isoenzyme of creatine kinase (CK-MB),aspartate aminotransferase(AST),alanine aminotransterase(ALT)was found in 63.6%,43.4%,38.0%,59.7%and 48.1%of the adult inpatients,respectively,42.7%,28.0%,20.0%,41.8%and 30.9% of juvenile patients,respectively,with the difference being statistically signifi cant(x2=10.38,5.95,6.73,7.59,7.27,P<0.01,0.05,0.01,0.01,0.01,respectively).Furthermore,theincrease of LDH activity correlated with the occurrence of complications(x2=12.60,P<0.01).Bronchopneumonia was diagnosed in 16.3%of adult inpatients with measles and 31.8%of juvenile inpatients(t=7.99,P<0.01).Conclusions Measles in adults tends to have a more severe clinical symptom but a better prognosis compared with measles in juveniles;however,this 1s often ignored in clinical practice.
8.Protective effect of resveratrol on UVA-irradiated human keratinocyte cell line and its mechanism
Mingliang CHEN ; Ji LI ; Lei SUN ; Xiao CHEN ; Dan JIAN ; Hongfu XIE ; Xiang CHEN
Chinese Journal of Dermatology 2008;41(11):745-748
Objective To observe the protection of human keratinocyte cell line, HaCaT cell, from UVA damage by resveratrol and its possible mechanism. Methods HaCaT cells were incubated with or without 0.01 mmol/L or 0.1 mmol/L resveratrol after exposure to 5 J/cm2 UVA irradiation. Unirradiated HaCaT cells-without the treatment with resveratrol served as the control. After another 24-hour culture, MTT assay was used to detect the proliferation of cells, RT-PCR and Western-blot to measure the iNOS mRNA and protein expression respectively, electron microscopic technique to observe the changes in cell ultrastructure. Results After irradiation with UVA of 5 J/cm2, the proliferation of HaCaT cells decreased with the absorbance at 490 nm descending from 0.889±0.083 to 0.542±0.004, while a significant increase was observed in the relative expression level of iNOS mRNA and protein in HaCaT cells (1.532±0.041 vs 0.009±0.003, 1.331 ±0.046 vs 0.003±0.001, both P < 0.05) with the presence of typical apoptotic cells. The treatment with 0.01 and 0.1 mmol/L resveratrol significantly promoted the proliferation of irradiated cells with the absorbance at 490 nm being 0.753±0.435 and 0.892±0.173 respectively, but inhibited the mRNA (0.853±0.038 vs 1.532±0.041, 0.392±0.033 vs 1.532±0.041, both P< 0.05) and protein expression level (0.809±0.018 vs 1.331±0.046, 0.412±0.026 vs 1.331±0.046, both P< 0.05) of iNOS in irradiated cells, and the resveratrol of 0.1 mmol/L was more effective than that ofO.01 mmol/L in all tested parameters (P< 0.05). Furthermore, no apoptofic cells or necrotic cells were observed in irradiated ceils incubated with resveratrol. Conclusion Resveratrol effectively protects HaCaT cells from UVA damage, which may be related to the inhibition of UVA-induced iNOS expression by resveratrol.
9.Expression of aquaporin 3 in four cutaneous tumors
Ji LI ; Bihong ZHANG ; Hongfu XIE ; Hua TANG ; Mingliang CHEN ; Xiang CHEN
Chinese Journal of Dermatology 2008;41(11):716-718
Objective To investigate the expression of aquaporin 3 (AQP3) in lesions of four cuta-neous tumors. Methods Immtmohistochemistry was utilized to measure the expression of AQP3 in tissue samples from 30 patients with seborrheic keratosis, 15 patients with Bowen's disease, 32 patients with squa-mous cell carcinoma, 17 patients with malignant melanoma and 15 normal human controls. Results AQP3 was observed in all the tissue samples from both patients and controls. A significant increment was noticed in the expression of AQP3 in patients with Bowen's disease, squamous cell carcinoma and malignant melanoma compared with the normal controls (all P < 0.01), while no significant difference was found between patients with seborrheic keratosis and the controls (P > 0.05). The highest expression of AQP3 was obtained in lesions from patients with squamous cell carcinoma and malignant melanoma, followed by those with Bowen's disease (both P < 0.01), and there was no significant difference between squamous cell carcinoma and malignant melanoma (P > 0.05). The differentiation status of squamous cell carcinoma significantly corre-lated with the expression of AQP3 (P < 0.01). AQP3 was significantly higher in malignant melanoma with metastasis than that in malignant melanoma without metastasis (P < 0.05). Conclusion The expression of AQP3 is upregulated in malignant skin tumors.
10.Ultrastructural change of chicken basilar papilla during a prolonged administration of kanamycin
Mingliang XIANG ; Hao WU ; Yun LI ; Zhihua ZHANG ; Qi HUANG ; Li CHEN
Chinese Journal of Tissue Engineering Research 2006;10(26):177-180,封三
BACKGROUND: Although some studies have been done on the functional change of cochlea in chickens during prolonged administration of kanamycin,few studies on ultrastructural changes in chicken cochlea have been reported.OBJECTIVE: To observe ultrastructural changes of the chicken basilar papilla following poisoning with prolonged administration of kanamycin.DESIGN: A completely randomized design and controlled observation.SETTING: Affiliated Xinhua Hospital, Shanghai Second Medical University.MATERIALS:The experiment was conducted in Xinhua Hospital Affiliated to Medical College, Shanghai Jiao Tong University from June 2001 to August 2003. Newly born Roman chickens (n=78), of either sex, provided by Shanghai Guixing Breeding Chick Farm, were used as subjects.METHODS: 200 mg/kg kanamycin was injected into muscles daily at day 3 after birth for 10 days. Then they were divided into 3 groups randomly:①typical application group: The chickens were applied with drugs no longer, and then killed at days 1, 3,7, 10 and 15, 6 chickens in each time phase. ②prolonged application group: 200 mg/kg kanamycin per day were injected into muscles continuously, and then killed at days 13, 17, 20, 25and 30, 6 chickens in each time phase. ③control group: The chickens were not treated with any drug. They were assigned into 3, 13 and 33 days groups with 6 chickens in each time phase. When the chickens were killed they were at the same age with the experime ntal animals at days 0, 10 and 30 of kanamycin treatment. All the experimental animals were studied with auditory brain-stem response (ABR) and scanning electron microscopy in chicken basilar papilla to observe ultrastructural change of cochlea, threshold, latency and amplitude of ABR wave I.MAIN OUTCOME MEASURES: ①Ultrastructural change of the chicken basilar papilla, and ② ABR result of chicken.RESULTS: A total of 78 Roman chickens were involved in the result analysis, without dropout. ①Outcome of ABR: Loss and recovery of chicken ABR threshold value was similar between within continuous administration for 20 days and administration for 10 days plus 10 days of recovery (drug withdrawal group). Twenty days after administration, ABR threshold significantly increased again. ②Outcome of scanning electron microscopy: At the end of 10-day kanamycin administration, hair cells in 40% proximal chicken basilar papilla were destroyedand disappeared completely. Although continuous administration was performed, the hair cells began to regenerate and repair after damage. Within the 20-day continuous administration, the regenerated hair cells of basilar papilla developed maturity, which was mostly coincident with the administration for 10 days group. At day 25, regenerated hair cells of proximal basilar papilla were injured once again. At day 30, most of the regenerated hair cells were destroyed and disappeared.CONCLUSION: Prolonged administration of kanamycin obviously prevents the full repair of chicken basilar papilla damaged by kanamycin poisoning.