Objective To explore the clinical significance of DSA and MRA detection in cerebrovascular desease of patients with diabetes mellitus.Methods 142 diabetic patients were selected in the study. The vascular structure was detected by digital subtraction angiography (DSA) and magnetic resonance angiography (MRA) . Results There was 1123 (56.5%) branches of artery atherosclerotic stenosis in a total of 1988 detecting artery and there was no significant difference between the DSA and MRA (χ2=0.36、0.47、0.53,P>0.05) . The results showed that the DSA and MRA methods had no significant difference on display average count in detecting bilateral ACA,MCA,PCA 284 vessels (t=0.27、0.56、0.89,P>0.05) . There were 656 arteries stenosis in checking the Willis ring vascular by DSA evaluation of the Willis ring and the sensitivity and specificity by MRA were more than 90%. Conclusion MRA and DSA can be applied in diagnosis of in cerebrovascular disease of patients with diabetes mellitus.The noninvasive advantages of MRA are worth to be concerned by clinician.

OBJECTIVE To know about the urogentital tract infection caused by Ureaplasma urealyticum(Uu) and Mycoplasma hominis(Mh) and distribution and antibiotic susceptibility.METHODS Uu and Mh were isolated and their antibiotic susceptibility was detected by ICS kit from Lizhu company in Zhuhai.RESULTS Among the 246 samples,126 were positive(51.22%) of which Uu were 114,Mh 2,and both Uu and Mh 10;females were more apt to be infected(P

Objective Analysis of the complete genome sequence about the newly emerged pandemic norovirus GⅡ.4 genotype, to understand its variation characteristics.Methods 264 patients were collected with diarrhea.The RNA was extracted from 264 fe-cal specimens and cDNA was synthetized.The positive samples were amplified by PCR,the amplified fragments were sequenced. The complete genome sequences of the norovirus was sequenced and analyzed.Results A new norovirus variant strain of Jingzhou GⅡ.4,a pleiston of Sydney GⅡ.4 was isolated.A large variation was found in the new variant subtype,which was mutated inclu-ding in the hypervariable P2 domain of the major capsid protein VP1.Conclusion The result demonstrates the variant strain of Sydney GⅡ.4 was spread to China.VP1 of norovirus GⅡ.4 is evolving rapidly.The spread and evolution situation of the norovirus GⅡ.4 need to be closely monitored in China for the development of effective vaccines and therapeutic monoclonal antibodies.

Objective To explore the value of automatic photography in the observation of results of Schistosoma japoni?cum miracidium hatching experiments. Methods Some fresh S. japonicum eggs were added into cow feces,and the samples of feces were divided into a low infested experimental group and a high infested group(40 samples each group). In addition,there was a negative control group with 40 samples of cow feces without S. japonicum eggs. The conventional nylon bag S. japonicum miracidium hatching experiments were performed. The process was observed with the method of flashlight and magnifying glass combined with automatic video(automatic photography method),and,at the same time,with the naked eye observation meth?od. The results were compared. Results In the low infested group,the miracidium positive detection rates were 57.5% and 85.0%by the naked eye observation method and automatic photography method,respectively(χ2=11.723,P<0.05). In the high infested group,the positive detection rates were 97.5%and 100%by the naked eye observation method and automatic pho?tography method,respectively(χ2= 1.253,P > 0.05). In the two infested groups,the average positive detection rates were 77.5% and 92.5% by the naked eye observation method and automatic photography method,respectively(χ2 = 6.894,P <0.05). Conclusion The automatic photography can effectively improve the positive detection rate in the S. japonicum miracidi?um hatching experiments.

Objective　To investigate the effect of congenital human cytomegalovirus（HCMV） infection on the growth of embryo and cerebral cortex. Methods　HCMV (6.0 log TCID50 in 1.0 ml/mice) was separately injected into the intraperitoneum of 10-week-old female mice on pre-mating, 3, 7, 15 gestation days. Cerebral cortices of fetuses were collected by laparotomy on 19th gestation-day just before delivery. The specimens were fixed with 4% buffered solution of paraformal dehyde. And then sectioned and stained with HE. Meanwhile, part of the specimens was used for viral isolation and HCMV DNA test by PCR. Results　In the cerebral cortex of fetal mice, it was found that the capillary was dilated and congested, and the parenchyma was infiltrated with neutrophils, monocyte for experimental group and not for control group. The degeneration, necrosis and apoptosis of neurons co-existed. The rates of virus isolation for the fetal cerebral cortex among pre-mating, 3-, 7-, 15-gestation-day groups were 60%、62%、67%、25%,respectively （χ2=13.475，P＜0.05) and the rate for 15 gestation-day group was significantly lower than others. Average weight of experimental mice for 15-gestation-day was significantly lower than control group and those for other gestation groups were not significantly different on group t-test. Conclusion　HCMV can replicate in the cerebral cortex of ICR mice, infect fetal and initiate congenital infection of cerebral cortex of fetus. In addition, embryo of different gestation stages can be infected by HCMV on experiment. HCMV delays embryo growth and causes the cerebral cortex damage.

Objective To study the evaluation between the X-ray findings and ultrasound(US) manifestations of upper digestiveobstruction in infancy.Methods X-ray and US manifestations of 20 cases of infant with upper digestive obstruction proved by operationwere analysed respectively.Results In 20 cases,12 patients had pyloric stenosis,3 duodenal stresia,3 midgut malformation,2 hiatalhernia.Pyloric stenosis was diagnosed by X-ray in 11 cases,by US in 9,misdiagnosed by US in 1 case.Duodenal stresia was observed by X-ray and US in 2 cases separately,not observed by X-ray and US in 1 case separately.Midgut malformation was demonstrated by X-ray and US in 1 case separately,not demonstrated in 2 cases separately.Hiatal hernia was found by X-ray in 2 cases,misdiagnosed by US in 2 cases.Conclusion The two methods can't substitute with each other but complement.X-ray is better than US in the diagnosis of pyloric stenosis and hiatal hernia.US is better than X-ray in the diagnosis of midgut malformation.

Objective To investigate the effect of platelet-derived growth factor(PDGF) in DCVS diseases after SAH. Methods Alteration of PDGF gene and mRNA expression in DCVS brain tissues after SAH by RT-PCR and immunohistochemistry. Results To compare with the control group, transcription level of PDGF mRNA up-regulated evidently 3 days after transfusion (P

Objective To evaluate the application and risk of hematogenous tumor dissemination of intraoperative autologous blood salvage on radical nephrectomy for renal carcinoma with inferior vena cava tumor thrombus.Methods Between July 1999 and October 2005,19 patients with renal cell carcinoma invading inferior vena cava were used by the intraoperative autologous blood salvage.Preoperative and postoperative clinical data were analyzed and compared.The blood sparing effect and follow-up were evaluated.Results The intraoperative autologous blood salvage of all cases was successful.The levels of HCT,Hb and RBC on the 1st and 7th postoperative day were significantly lower than preoperative day.Although the level of PLT decreased on the 1st postoperative day,it recovered on the 7th day.The markers of liver and renal function did not change during treat time.Packed red blood cells were collected during surgery with a mean value of 1 765 ml(300～6 000 ml) per patient.The average amount of allogenic transfusion per patient was 2 247ml(0～8000 ml).The perioperative mortality rate was 10.5%(2/19).The postoperative metastasis rate was 35.3%(6/17).No difference was found in the prognosis between two groups.Conclusion The intraoperative autologous blood salvage does not markedly change the clinic test data and not increase the recurrence and metastasis of renal cell carcinoma.

Objective To explore the curative effect of insulin combined with An Er Shu in treatment ofⅡtoⅢ degree pressure sores of elderly patients with diabetes mellitus. Methods A total of 120 cases of elderly diabetes mellitus patients with Ⅱ to Ⅲ degree pressure sores were randomly divided into the control group (30 cases, conventional nursing treatment), the experimental group 1 (30 cases, insulin spray coating), the experimental group 2 (30 cases, An Er Shu brushing besmear), and the experimental group 3(30 cases, insulin combined An Er Shu). The curative effect and the healing time were observed. Results After four weeks treatment total effective rate was 60.0%(18/30) in the control group, 66.7%(20/30) in the experimental group 1, 76.7%(23/30) in the experimental group 2, 100.0%(30/30) in the experimental group 3, and there was significant difference in the 3 experimental groups comparing with the control group, the experimental group 1 and the experimental group 2, respectively (χ2=15.000, 12.000, 5.822, P<0.05 or 0.01). The healing time ofⅡdegree pressure sores was (19.03 ± 0.85) d in the control group, (18.90 ± 0.92) d in the experimental group 1, (18.43 ± 0.82) d in the experimental group 2, and (16.97 ± 1.25) d in the experimental group 3, and there was significant difference in the experimental group 3 comparing with the control group, experimental group 1 and experimental group 2, respectively (t=8.013, 7.918, 8.930, P<0.01), and in the experimental group 2 comparing with the control group (t=3.525, P<0.01). The healing time ofⅢdegree pressure sores was (24.17 ± 1.51) d in the control group, (23.63 ± 1.33) d in the experimental group 1, (23.47 ± 1.25) d in the experimental group 2, and (21.07 ± 1.46) d in the experimental group 3, and there was significant difference in the experimental group 3 comparing with the control group, experimental group 1 and experimental group 2, respectively (t=6.918, 7.048, 9.200, P<0.01). Pressure sores area reduction was (44.47 ± 37.63)%in the control group, (56.50 ± 39.64)%in the experimental group 1, (66.23 ± 37.54)%in the experimental group 2, and (96.52 ± 7.71) % in the experimental group 3, and there was significant difference in the experimental group 3 comparing with the control group, the experimental group 1 and the experimental group 2, respectively (Z=-4.274,-4.274,-3.400, P<0.01). Conclusions Insulin combined An Er Shu in treatment of pressure sores in elderly patients with diabetes mellitus can improve curative effect and shorten the healing time.

OBJECTIVE To study the effect of frequency difference and intensity difference on mismatch negativity (MMN) change in normal persons. Emphatically to observe the effect on the latency and amplitude of normal people's MMN, as well as the threshold of the acoustic difference which may induce the MMN. METHODS Twenty-four adults were included in this study. Their thresholds of pure tone audiometry were within 20dBHL. The MMN were measured by using the Smart EP ( ear potentiometer provided by the company of Intelligent Hearing) and the rule of the change by the difference of frequency and intensity to the latency and amplitude of normal people’s MMN were studied. RESULTS Along with the decreasing of the difference of the frequency, the MMN latency prolonged gradually. The amplitude of the MMN decreased gradually as the decreasing of the intensity difference. CONCLUSION A stable MMN graph is an important indicator of objective physiological test with hearing distinguish.