Objective To observe the curative effects of Guillain-Barre Syndrome(GBS) treated with large dose of immunoglobulin.Methods 34 patients with acute GBS were randomly divided into intravenous immunoglobulin(IVIG) group and control group(17 cases in each group).The patients in IVIG group were treated with intravenous injection of human immunoglobulin 0.4 g/(kg?d) for five days,while the other treatment of two groups was similar.The curative effects was evaluated at 21 days after therapy.Results The cure-rate(52.9%) and total effective rate(100%) in IVIG group were signifcantly higher than those in control group(11.8% and 76.5%)(all P

Biodegradable nano/microparticles of poly(D, L-lactide-co-glycolide) (PLGA) is a novel non-viral gene vector, which has many advantages, such as safety, non-immunogenicity, easy of large-scale preparation and well load-capability. Therefore, more and more attentions and researches have been paid on its application. Especially, PLGA has shown enormous potential application value and space in the field of plasmid DNA (pDNA) delivery system. On the basis of the current situation of PLGA nano/microparticles for pDNA delivery, this paper focused on summarizing the current preparation approaches and surface modified methods of PLGA particle, furthermore showing its application in gene therapy and genetic vaccine delivery. These showed that PLGA nano/microparticles have extensive prospect in the development of controlled gene delivery system.

Object To study the change of serum soluble interleukin 2 receptor(SIL-2R) in syphilitic patients and its clinical significance.Methods The serum SIL-2R level in syphilitic patients of different stages and after therapy were detected using the ELISA technique.Results The serum SIL-2R level in syphiliti patients was higher than that in control group(P0 05).Conclusions SIL-2R level and its variation have some reference value for evaluating the therapeutic effect and judgement of the disease course in syphilitic patients.

Objective To investigate the relationship between impaired glucose test and insulin resistance in pregnancy. Methods Ninety-three pregnant women who had one abnormal result among 4 of oral glucose tolerance test (OGTT) and 179 pregnant women who had normal results of OGTT from 11 hospitals of Shanghai during May 1999 to Aug 1999 were included. Insulin release test were performed at the same time. Insulin sensitive index (ISI), islet secretive function index (IFI) were calculated. Area under sugar curve (SAUC)?area under insulin curve (IAUC)?ISI? IFI?fast plasma sugar(PGO) and fast plasma insulin (INO) were compared between two groups. Results PGO, INO, SAUC, IAUC and ISI of the IGT group and control group were(5.1?1.0)mmol/L vs(4.3?0.4)mmol/L?(142?77) pmol/L vs (114?40)pmol/L?(1186?437) pmol/L vs (1058?407)pmol/L?(23?4) mmol/L vs (18?3)mmol/L and 30?20 vs 22?24 respectively, the differences were significant(P0.05). Conclusions Insulin resistance is one of the causes of impaired glucose test in pregnancy.

Objective To investigate whether insulin resistance is a cause of pregnancy induced hypertension (PIH). Methods All patients who had abnormal 50g glucose screening test accepted 75g oral glucose tolerance test (OGTT) and insulin sensitive index (ISI) was calculated. These patients were followed up and divided into two groups (PIH group and normal pregnancy group) at third trimester of pregnancy. The difference of ISI between the PIH group and normal pregnancy group was compared. Results Fast blood glucose, fast blood insulin and ISI were (4.2?0.7)mmol/L vs. (3.8?0.7)mmol/L, (107.8?48.8)pmol/L vs. (50.4?40.5)pmol/L, and -3.25?0.27 vs. -2.58?0.66 in PIH group and normal group, respectively ( P 0.05). Conclusions Insulin resistance during second trimester of pregnancy may be one of the causes of pregnancy induced hypertension.

ZHANG Lin-hua, GAO Rui-chang, XU Ming-li (School of Chemical Engineering, Tianjin University, Tianjin 300072, China)

OBJECTIVE: To evaluate the effect of intervention measures on perioperative preventative application of antibiotics. METHODS: 120 surgery patients in our hospital were collected and studied comparatively before (Jul.～Dec. of 2006) and after carrying out intervention measures (Jul.～Dec. of 2008). RESULTS: The rational utilization rate of antibiotic during perioperative period increased from 0% (before intervention) to 56.7 % (after intervention). Average admission day and average medication duration after operation both were shortened (P

Acute nonvariceal upper gastrointestinal bleeding (ANVUGIB)is a commonly seen gastrointestinal emergency.Rockall and Blatchford scoring system are commonly used for risk stratification in ANVUGIB.Aims:To investigate the predictive values of Rockall and Blatchford scoring system for assessing the risk of blood transfusion,surgical intervention and mortality in patients with ANVUGIB.Methods:Five hundred and ninety hospitalized patients with ANVUGIB were scored by Rockall and Blatchford scoring system,respectively.Predictive values of these two scoring systems for assessing the risk of blood transfusion,surgical intervention and mortality were assessed by area under the receiver operating characteristic (ROC)curve (AUC).Results:Rockall and Blatchford scores in patients with blood transfusion,surgical intervention and died were significantly higher than those in patients without blood transfusion,surgical intervention and survived (P <0.01 ).The AUC of Rockall scoring system for predicting blood transfusion,surgical intervention and mortality were 0.785 (95% CI:0.743-0.828,P=0.000),0.765 (95% CI:0.693-0.837,P=0.000),0.835 (95% CI:0.703-0.966,P=0.005),respectively.The AUC of Blatchford scoring system for predicting blood transfusion,surgical intervention and mortality were 0.812 (95%CI:0.775-0.848,P=0.000),0.870 (95%CI:0.811-0.930,P=0.000),0.784 (95% CI:0.614-0.954,P=0.017),respectively.Conclusions:Rockall and Blatchford scoring system have high predictive value for blood transfusion,surgical intervention and mortality in patients with ANVUGIB.Rockall scoring system is better for predicting mortality,while Blatchford scoring system is better for predicting blood transfusion and surgical intervention.

Objective To compare sleep electroencephalogram-modulated repetitive transcranial magnetic stimulation (SEM-rTMS) with routine repetitive transcranial magnetic stimulation (R-rTMS) in the treatment of primary insomnia. Methods One hundred and twenty six patients with insomnia were divided randomly into a SEM-rTMS group (44 cases) , a R-rTMS group (42 cases) and a sham rTMS group (40 cases). Each case was treated with rTMS for 30 min once a day for 10 d under double blinding of the patient and therapist. The clinical effect was observed along with Krakow sleep scores, electroencephalograms and blood pressure before and after a 10-day course of therapy and 30 days later. Results During rTMS treatment, some patients reported feeling sleepy, and this was confirmed by their EEGs at the time. After the entire course of therapy, 80% of the SEM-rTMS group and 45% of the R-rTMS group showed improvements, a significant difference. At the 30th day after therapy, the effect in the SEM-rTMS group was still significantly better than in the R-rTMS group. There were no obvious side effects in any group throughout the whole observation period. Conclusions SEM-rTMS is more effective and more reliable than R-rTMS. SEM-rTMS is safe to use with primary insomnia patients.

Objective To investigate the value of retraction phenomenon in coronal plane at threedimensional ultrasonography(3D-US) in preoperative assessment of prognostic factors in breast cancer.Methods Surgical resection specimens of 66 malignant breast lesions in 66 women who had undergone 3D-US were included.The presence of the retraction phenomenon in the coronal plane was evaluated.Pathologic prognostic factors,including tumor size,histologic grade,lymph node status,estrogen receptor (ER) and progesterone receptor (PR) status,and human epidermal growth factor receptor 2 (Cerb-B2) and p53 expression were determined.Correlation of the retraction phenomenon and prognostic factors was analyzed.Results The retraction phenomenon was correlated to the tumor size,histologic grade,and estrogen and progesterone receptor status.Compared with the breast cancer without the retraction phenomenon,the tumor foci with the retraction phenomenon were more likely to show a smaller diameter less than 2 cm (73.7％ vs 46.4％,P ＜0.05),a lower histologic grade with grade Ⅱ (88.2％ vs 42.9％,P ＜0.01),and a higher positive rate of ER and PR (86.8 ％ vs 64.3 ％,P ＜0.05;81.6 ％ vs 57.1％,P ＜0.05).The lymph node status and the expression of the CerB-2 and p53 in the two groups had no significant difference (P ＞0.05).Conclusions The retraction phenomenon in the coronal plane of three-dimensional US may be useful in the noninvasive prediction of prognostic factors of breast cancers.