The electronic structure of a Lindqvist type [Mo7O24]6- anion with 5 different types of counterion (K+, Na+, NH4+, [NH3Pr]+ and [NH3Pri]+) was calculated by using the Discrete Variational Method coupled with Density Functional Theory (DFT-DVM). It could be concluded through variance analysis to the calculated results that the type of counterion does not influence remarkably the electronic structure of [Mo7O24]6- anions. Perhaps it can be used to explain the experiment fact that the polyoxomolybdate structure of the Mo7O24 framework is apparently of critical significance to antitumor action. On these grounds we forecast that two other lindqvist type heptamolybadates(their counterions are Na+ and [NH3Pr]+ respectively) may also exhibit antitumor activities.
Antineoplastic Agents ; chemistry ; Ions ; chemistry ; Molybdenum ; chemistry

In recent decades, bone tissue engineering has made great progress in the treatment of large bone defects, among which bioprinting is one of the most important technologies. 3D bioprinting achieves precise control of the spatial structure of bone tissue engineering scaffold manufacturing by adding different materials in layers, and cells are placed into the scaffolds based on hydrogel materials to solve the uniform distribution of cells in the scaffolds. However, most biomedical materials used for 3D bioprinting are static and cannot be changed with the dynamics of the body's internal environment. 4D bioprinting combines the concept of time with 3D bioprinting and uses stimulus-responsive materials to change their shape under various stimuli to create dynamic 3D biological structures. It offers unprecedented potential for bone tissue engineering. The shape memory properties of printed structures meet the needs of personalized bone defect repair, and functional maturation procedures promote osteogenic differentiation of stem cells. In this paper, we review the commonly used 3D bioprinting methods and the mechanism of functional and morphological transformation in 3D bioprinting developed into 4D bioprinting technology by summarizing the research on bioprinting and tissue engineering at home and abroad in recent years. What's more, we introduce the application of bioprinting in the treatment of bone defects in bone tissue engineering as well as the current challenges and future prospects.

Objective:To explore the effects of group counseling combined with staged health education on knowledge-attitude-practice and psychological condition in family members of leukemia children.Methods:From January 2017 to October 2018, we selected a total of 200 family members of leukemia children in the First Affiliated Hospital of Zhengzhou University as subjects. All of them were divided into observation group and control group according to admission time, 100 cases in each group. Control group provided staged health education for children's family members, while observation group carried out staged health education combined with group counseling. The knowledge-attitude-practice, psychological condition and satisfaction with health education of family members of two groups were evaluated with the Leukemia Knowledge-Attitude-Practice Questionnaire, Hamilton Anxiety Scale (HAMA) , Hamilton Depression Scale (HAMD) and the Satisfaction Questionnaire.Results:The scores of leukemia knowledge and practice of observation group were higher than those of control group with statistical differences ( P<0.01) ; the master rate of knowledge-attitude-practice of observation group was higher than that of control group with a statistical difference ( P<0.01) . After intervention, the scores of HAMA and HAMD of family members between two groups all decreased, and the decline range of observation group was higher than that of control group with a statistical difference ( P<0.01) . The satisfaction with health education of observation group and control group was 98.00% and 86.00% with a statistical difference ( P<0.01) . Conclusions:Counseling combined with staged health education can improve knowledge-attitude-practice, anxiety, depression as well as health education satisfaction of family members of leukemia children.

Objective:To explore the status quo and influencing factors of anxiety among caregivers of children with cancer during chemotherapy to provide a reference for clinical intervention.Methods:In this cross-sectional study, 133 caregivers of children with cancer during chemotherapy admitted at the First Affiliated Hospital of Zhengzhou University between May and August 2020 were selected by convenient sampling and investigated with the General Information Questionnaire, Generalized Anxiety Disorder-7 (GAD-7) , and Chinese version of Cognitive Fusion Questionnaire (CFQ) . In this study, a total of 133 questionnaires were distributed and 124 valid ones were returned, accounting for an effective response rate of 93.2%.Results:The GAD-7 score of the 124 caregivers was 10.50 (6.00, 15.75) ; the incidence of anxiety was 84.68% (105/124) ; and the Chinese version of CFQ score was 42.00 (32.00, 50.00) . There was a positive correlation between the GAD-7 and CFQ scores ( r=0.639, P<0.01) . Binary logistic regression analysis showed that cognitive fusion was a risk factor for anxiety among caregivers of children with cancer during chemotherapy ( P<0.01) . Conclusions:The anxiety and cognitive fusion levels among caregivers of children with cancer during chemotherapy are relatively high and positively correlated with each other. Targeted interventions should be provided in clinical practice for the cognitive fusion of caregivers of children with cancer during chemotherapy to reduce their anxiety and improve the quality of care for children.

OBJECTIVETo explore the association of a functional polymorphism Val158Met of COMT gene and attention and executive function in first-episode treatment-naive patients with schizophrenia and healthy controls.

METHODSTrail making test (TMT) and clinical performances were evaluated in 103 first-episode treatment-naive patients with schizophrenia and 99 healthy controls. Polymorphism of COMT Val158Met was analyzed using polymerase chain reaction-restriction fragment length polymorphism method. A general linear model was used to investigate the effect of genotype subgroups on the attention and executive function.

RESULTSThere was a significant difference between control subjects and patients with schizophrenia on the TMT-A and B. However, no significant difference among Val/Val, Val/Met and Met/Met on the TMT-A and B in control subjects and patients with schizophrenia was detected.

CONCLUSIONThe association among COMT Met variant and trail making testing (attention and executive function) has been replicated. However, no association of COMT Met variant with disruption of dopaminergic influence on neurocognitive function was detected. This may be due to the heterogeneity of population.

Adolescent ; Adult ; Amino Acid Substitution ; Attention ; physiology ; Catechol O-Methyltransferase ; genetics ; Executive Function ; physiology ; Female ; Gene Frequency ; Genotype ; Humans ; Male ; Polymerase Chain Reaction ; Polymorphism, Genetic ; Polymorphism, Restriction Fragment Length ; Schizophrenia ; genetics ; physiopathology ; Schizophrenic Psychology ; Trail Making Test ; Young Adult

Low-intensity pulsed ultrasound(LIPUS)was first introduced in clinic for the treatment of fracture because of its safety,ef-fectiveness and non-trauma.Then,a large number of subsequent studies have used LIPUS as a synergistic factor in bone,cartilage,joint,cancer treatment and oral clinical research,jointly revealing the powerful synergistic effect of LIPUS.This paper summarizes the synergistic effect of LIPUS in the above research fields,in order to broaden the possible clinical application scope of LIPUS.

ObjectiveTo explore the correlation between post-traumatic stress disorder(PTSD) and emotional regulation in the parents whose children have acute lymphoblastic leukemia(ALL). MethodsBy convenient sampling method, a total of 233 parents whose children had ALL were selected in the First Affiliated Hospital of Zhengzhou University from June 2013 to June 2017, and the levels of PTSD and emotional regulation were evaluated by The PTSD Checklist-Civilian Version (PCL-C) and Emotion Regulation Questionnaire(ERQ). A total of 233 questionnaires were distributed and 233 valid ones were retrieved, yielding an effective recovery rate of 100%. ResultsIn this study, the participants got (35.82±4.93) points for PCL-C, among which the highest average item score was (2.29±0.36) points for "Avoid/Numb"; the score of "cognitive reappraisal" and"expressive suppression" were (4.27±0.64) and (4.35±0.60) points in ERQ. Pearson correlation analysis showed that the total score and each dimension score in PCL-C were negatively correlated with cognitive reappraisal (P＜0.05), and were positively correlated with expressive suppression (P＜0.05). Multivariate linear regression analysis showed that cognitive reappraisal and expressive suppression were the influencing factors for PTSD of the parents with ALL children (P＜0.05). ConclusionsALL children's parents have serious PTSD and tend to choose the emotional regulation way of expressive suppression. There is correlation between the degree of PTSD in ALL children's parents and their emotional regulating ability, therefore, nursing staff should assist them in the improvement of their emotional regulation ability to reduce the level of PTSD.

Objective:To explore the effect of the training model based on the National Telemedicine Center in the technical training of children's implantable venous access port.Methods:Using the convenience sampling, 42 nurses who participated in the technical training of children's implantable venous access port in the First Affiliated Hospital of Zhengzhou University from May 2019 to December 2019 were selected as the study group. A total of 36 nurses who participated in the technical training of children's implantable venous access port in the First Affiliated Hospital of Zhengzhou University from December 2018 to March 2019 were retrospectively selected as the control group. The control group received the traditional training, and the study group implemented the training model based on the National Telemedicine Center. The theoretical assessment, operational assessment and training satisfaction were compared between the two groups before and after training.Results:After training, the theoretical assessment, operational assessment and training satisfaction scores of the study group were higher than those of the control group, and the differences were statistically significant ( P<0.05) . Conclusions:The training model based on the National Telemedicine Center in the technical training of children's implantable venous access port can enhance the professional knowledge and practical skills of nurses and improve the training satisfaction.

Objective:To analyze the epidemiological and etiological characteristics of a death case of meningococcal meningitis in Hengyang city, Hunan Province in 2024.Methods:Epidemiological investigation of the death case was performed, and samples from the patient and close contacts were collected. Following cultivation and isolation, Neisseria meningitidis ( Nm) strains were analyzed by antimicrobial susceptibility testing, pulsed field gel electrophoresis (PFGE), and whole-genome sequencing for analyzing epidemiological and etiological characteristics. Phylogenetic analysis was carried out using core genomic multilocus sequence typing (cgMLST). Results:The case was a 16-year-old high school boarding student with fulminant meningococcal meningitis. He had shock symptoms, and died within 24 h of the onset of symptoms. Six Nm strains were isolated from the patient and his roommates, belonging to two distinct clades. Isolate 144569 from the patient was highly homologous to isolate 144572 from a close contact, both belonging to the highly pathogenic sublineage L44.1 of CC4821. The typical molecular features was C: P1.7-2, 14: F5-101: ST4821 (CC4821). The two strains carried the antimicrobial resistance genes of gyrA-71 and penA-552, indicating reduced susceptibility to quinolone and penicillin, which was with their resistance phenotype. The isolates from four close contacts clustered within the same clade, characterized by the molecular features of B: P1.18-25, 9-18: ST5829 (UA). Conclusions:The death case is caused by Nm serogroup C from highly pathogenic sublineage L44.1 of CC4821. The spread of this isolate has the potential risk of outbreaks of invasive meningococcal disease. It is necessary to enhanced the molecular epidemiological surveillance, particularly focusing on the transmission of multiple serogroups of Nm among adolescents and the increasing exposure risk.