Objective To study the value of the liver and spleen interventional therapy in primary carcinoma of the liver with hyperspleenism. Methods The treatment was given to 25 patients. Using the method of Seldinger,the 5FRH into artery hepatica propria to infuse 2/3 of the overall dose of chemotherapy medicine and embolism was inserted into the liver segment. Then 1/3 of it was infused into artery lienalis and gelatin sponge was infused into artery lienalis Rr. lienales. Combined regimens were used in chemotherapy: 5-Fu 750mg, EADM 40mg, MMC 10mmg,and 10 of the 25 added DDP 40mg. LUF and gelatin sponge were used for embolisation. Results Of the 25 patients,the WBC and PLT increased in 25 patients 4 weeks after the operation. The difference was significant. The survival rate at 6 and 12 months was 100% and 60% respectively,with 1 case of CR,8 cases of PR and 6 cases of SD. Patients had fever after the treatment but few with abnormal liver function tests. Conclusion Liver and spleen interventional therapy may relieve hyperspleenism and suggests that the treatment of primary carcinoma of the liver is effective.

Objective To investigate the significance of human cyomeg Movirus(HCMV)pp65 IgG antibody avidity index(AI)for the clinical diagnosis of HCMV primary infection through the experimental model of HCMV primaly infection in BALB/c mice.Methods 6～8 weeks,female,specific-pathogen-free BALB/c mice were divided into 5 groups.6 mice in each group. And he mice were injected with 2×106 PFU/m1,2×105 PFU/mi,2×104 PFU/ml,2×103 PFU/ml and 2×102 PFU/ml of HCMV intraperitoneally respectively. Another 6 mice were injected intraperitoneally with the maximum dose of HCMV kept at 56℃ for 30 min as inactivated virus group.And HF negative control group was established at same time. All the mice ere sacrificed to obtain brain and lung tissues for the following experiments after 1 montll.(1)Tissue samples obtained from mice were inoculated in human embryo fibroblasts(HF)monolayers after routine treatment for virus isolation.HCMV specific eytopathie effect(CPE) was observed bv inverted phase-contrast microscopy.HCMV UL83 DNA in the ultures as tested by PCR and pp65 antigen was detected by indirect immunofluorescence.(2)Extracted mRNA from tissue samples and HCMV pp67 mRNA were analyzed by reverse transcriptase PCR(RT-PcR).(3)Immunoglobulin M(IgM)antibody and immunoglobulin G(1gG)antibody avidity Was investigated for their usefulness in distinguishing primary genital HCMV infections rom nonprimary infections with ELISA kit using truncated pp65 protein.ResultsHCMV can be isolated in the tissues from the mice injected with 2×106 PFU/ml and 2×105 PFU/m1.RT- PCR and ELISA showed positive results in the same groups.The infective rates were 100%.The analysis of the low doses groups,inactivated group and HF negative ontrol group all showed negative results.Conclusions BALB/c mice can be infected with HCMV and appeared as primary infection after1 month.Determination of HCMV pp65 IgM and HCMV p065 IgG-AI by ELISA incorporated with virus isolation and RT-PCR are helpful for distinguishing primary infections from nonpfimary infections.The detection of HCMV p65 IgM and HCMV pp65 IgG-AI by ELISA utilizing recombinant protein pp5 as antigens can be used for preliminary screening.

Objective To establish a new ELISA method by use of shorted recombinant antigen pp65 for detection of IgM antibodies to human cytomegalovirus (HCMV).Method According to HCMV pp65 sequence of nucleotide and amino acid, the shorted pp65 was decided by computer soft ware of protein and attained through gene engineering technique. The by computer soft ware of protein and attained through gene engineering technique. The recombinant enzyme- linked immunosorbent assay (REC-ELISA ) method was developed using pp65 recombinant protein as antigen and was applied to detecting anti-HCMV-IgM in sera. It was compared to ELISA by use of whole virus as antigen and Biocheck CMV IgM enzyme immunoassay test kit.Results To detect HCMV IgM by REC-ELISA, the best quantity of pp65 was 3.5?g percent cavity, the best dilution of HRP~*anti-HCMV IgM was 1∶[KG-*2]800 and that of serum was 1∶[KG-*2]100. The positive coefficient of variation (CV) was 9.5% in stability assay. The average CV of Inter- assay and Intra-assay was 4.5% and 9.6% respectively. The positive value of REC- ELISA was 44%, that of ELISA using whole virus was 50% and BioCheck was 45%. REC-ELISA using most suitable condition was concordant with BioCheck, which was 97.0%. Its youden′s index was 92.8% and its specificity (98.2%) was higher than that of ELISA using whole virus as antigen (90.9%), too.Conclusion REC-RLISA had good sensitivity, specificity and reproducibility. The method was easy and quick. Recombinant protein was harmless and easy gained compared with the whole virus. It can be large-scale production and standardization. Therefore, the application value and potential of REC-ELISA was very large.

Objective:To survey the continuous change features of cusp angle in anatomic artificial teeth,and to investigate their effects on occlusal balance.Methods:28 artificial teeth of a complete denture were measured by optical three-dimensional(3D)scan-ner and the corresponding digital models were constructed using the reverse engineering software Surfacer 1 0.5.The working side cusp slopes of lateroprotrusive movement including the maxillary buccal cusp distal slopes and the mandibular buccal cusp mesial slopes were studied.The straight lines from bottom to top of cusp were constructed and the corresponding projected curves in occlusal surface were generated.The angulation values of straight line and the continuous change values of projected curve were calculated.Results:The angulation values of artificial teeth cusp inclination including straight line and corresponding projected curve were obtained.The maxillary working cusp inclination varied from 1 3.7°to 25.6°,and the mandibular working cusp inclination varied from 1 1 .3°to 37.7°.Conclusion:The relatively large differences of the cusp inclination among traditional anatomic artificial teeth and the convex form of the cusp are disadvantage factors to set up stable centric stop and unhindered chew course.

Objective To define that human cytomegalovirus (HCMV) can cross the placenta of the Balb/c mice and induce liver damage in developing fetus. Methods HCMV AD169 (6.0 logTCID 50 , 3.0 logTCID 50 , 1.5 logTCID 50 per mouse respectively) was injected into the peritoneum of mice (half of mice were female) when they were about 10 weeks old (weight 25 30g). Then, these mice were paired to mate. Fetus on day about to give birth was removed from the uterus and liver was obtained for virus isolation, pathological studies, examination of the viral DNA positive cells by in situ hybridization using digoxigenin labelled HCMV DNA oligonucleotide probe. Results HCMV could be isolated from the supernatant of tissues; HCMV DNA was found by PCR in supernatant of cell culture with CPE; the presence of viral DNA sequence in hepatocytes was confirmed by in situ hybridization; pathological changes in liver consist of swollen cytoplasm and destroyed nuclei of hepatocytes and distinct intranuclear inclusion in hepatocytes with a cellular infiltrates of predominantly phagocytic cells. All above were found more obviously in fetal mouse liver tissues from the group inoculated with 6.0 log TCID 50 HCMV AD169 as compared with 3.0 log TCID 50 group. In contrast, these positive results couldn't be well found in 1.5 log TCID 50 group. Nothing could be found in normal controls. Conclusions Our research suggests that primary maternal HCMV infection during pregnancy could induce congenital infection in fetus by transplacental transmission and induce fetal liver damage. The mouse model will provide the basis for the study on pathogenesis of congenital HCMV infection in liver and the development of prevention, diagnosis and antiviral agents for congenital HCMV infection in human being.

Objective: To investigate the roots and canals morphology o f extracted maxillary first premolars. Methods: The root morphol ogy and the root canals of 115 extracted maxillary first premolars were visualiz ed on radiographs taken in the mesio-distal direction, then the teeth were cut transversely and root sections were examined, the root canal systems was analyse d with Vertucci’s classification. Results: 74(64%) teeth were w ith one root, 41(36%) with two roots, all teeth with two roots had two canals a nd each canal with one apical foramen,〔TypeⅠ(1)〕.39% of the single-root teet h demonstrated one canal, whereas 61% of the single-root teeth had two canals, 〔TypeⅡ(2-1), TypeⅣ(2) and TypeⅤ(1-2)〕.Conclusions: The roots of maxillary first premolars possesses a variety of canal system types.

Objective: To realize the requirement of adjacent area accuracy,axial surface physical contour and smoothness in the process of inlay computer-aided design(CAD).Methods:A compromised method was brought forward that some assistant 3-D constraint lines from occlusion to gingiva were used to lead the inlay contour on axial corner and other characterized areas,and two perpendicular 3-D constructing lines at adjacent area were used to construct accurate 3-D configuration of this area.The axial surface was completely defined by the network of specified curves which were two constructing lines and four borders.Results:Inlay axial surface was smooth,the contour was consistent with the residual tooth body and the 3-D configuration in adjacent area was accurate.Conclusion:It is feasible to realize the inlay axial surface accuracy,smoothness,and consistency using the method of special distributed 3-D B-Spline curve network.

Objective To compare the efficacy and toxicity of late-course concurrent radiochemotherapy and sequential chemoradiotherapy for advanced esophageal carcinoma. Methods Eighty-two patients with advanced esophageal carcinoma were randomized into two groups: 41 cases in late-course concurrent radiochemotherapy (LCRC) group were received two cycles chemotherapy and then underwent concurrent radiochemotherapy;41 cases in sequential chemoradiotherapy (SCR) group were received four cycles chemotherapy and then underwent radiotherapy. The regimen of chemotherapy in all cases:cisplatin 25mg/m2, 1-3 d;calcium folinate (CF) 150 mg/m2, 1-5 d;fluorouracil 375 mg/m2, 1-5 d, 21 d was one cycle. All patients were received the three-dimensional conformal radiation therapy, the total dose of radiation was same as 64 Gy. Results The short-term response rate was 85.4%(35/41) in LCRC group and 65.9%(27/41) in SCR group, they had significant difference ( P<0.05). The rates of acute radiation esophagitis that need treatment was 90.2%(37/41) in LCRC group and 87.8%(36/41) in SCR group, there had no significant difference (P>0.05). The l year, 2 years, 3 years survival rate were 68.3%(28/41) and 65.9%(27/41), 56.1%(23/41) and 51.2%(21/41), 46.3%(19/41) and 36.6%(15/41) respectively,the median survival time were 30.0 months and 26.0 months, there had no significant difference ( P>0.05). Conclusion The short-term efficacy of advanced esophageal carcinoma could be improved by the late-course concurrent radiochemotherapy.

OBJECTIVE To know about the urogentital tract infection caused by Ureaplasma urealyticum(Uu) and Mycoplasma hominis(Mh) and distribution and antibiotic susceptibility.METHODS Uu and Mh were isolated and their antibiotic susceptibility was detected by ICS kit from Lizhu company in Zhuhai.RESULTS Among the 246 samples,126 were positive(51.22%) of which Uu were 114,Mh 2,and both Uu and Mh 10;females were more apt to be infected(P