Hepatitis B virus (HBV) infection is a major global public health problem. Persistent HBV infection is prone to develop chronic hepatitis B (CHB), and CHB is closely related to the development of liver fibrosis and hepatocellular carcinoma. High-affinity specific anti-HBs are essential for the control of HBV infection, while the antibody production is closely related to follicular helper T (Tfh) cells. Tfh cells can help B cells differentiate into plasma cells to produce specific antibodies to control virus infection. This article reviews the latest research progress of Tfh cells in HBV infection to provide information of new strategies for the prevention and treatment of HBV.

Objective:To evaluate the clinical efficacy of off-midline closure technique in the treatment of chronic pilonidal sinus.Methods:From July 2014 to July 2018, 59 patients suffered from chronic pilonidal sinus treated by off-midline closure were admitted to the Department of Proctology, Linyi Central Hospital, including 34 males and 25 females. According to the size and scope of the chronic pilonidal sinus, the off-midline flap was designed and sutured.Results:In this group of 59 patients, there were 52 cases of primary healing and 7 cases of incision complications, such as partial incision dehiscence and subcutaneous effusion. After follow-up for 2 years, no recurrence was observed. The scar was not obvious after wound healing. The patient was satisfied with the postoperative appearance.Conclusions:Using the off-midline closure technique to treat chronic pilonidal sinus can deviate from the midline to make the suture tension-free and the gluteal groove elevated. It not only effectively reduce the complication rate in patients, but also has high cure rate, low recurrence rate and does not affect appearance.