Objective To study the pathogenic characteristics of hepatic depression syndrome (HDS) through clinical epidemiologic survey. Methods By using the method of epidemiologic cluster sampling, the cases complicated with HDS from the hospitalized cases from January 2000 to December 2001 of the First Affiliated Hospital, the Second Affiliated Hospital and the Third Affiliated Hospital of Hunan University of TCM, and survey forms were fiUed. The data was counted and analyzed by using SPSS statistical analysis software. Result By the method of epidemiologic survey, the pathogenic characteristics of HDS were demonstrated as follows: universality-HDS could occur in many dieases, complexity-HDS often changed into other syndromes, orientation-HDS often influenced the function of spleen and stomach. Conclusion The method is an effective way to study pathogenesis of TCM. It is objective, truthful and consistent with the clinical situation.

ObjectiveTo Investigate the influencing fators of somatoform disorder and combidity with depressive-anxiety disorders in general hospital patients.MethodsA multi-center,hospital-based cross-sectional study was conducted.A total of 2044 subjects were screened by using general questionnaire,self-made investing scale related somatoform disorder,hospital anxiety and depression scale(HADS) and general health condition survey.Hamilton anxiety scale (HAMA),hamilton depression scale (HAMD),mini international neuropsychiatric interview (MINI)was used to evaluate by psychiatrists for the subjects who scored≥ 9on HADS.Results 16.24％ of all the patients with stamic have been diagnosed and confirmed with depression and anxiety.Possible causes for depression and anxiety included family depression history(P＜ 0.001,OR=83.481 ),past incidence of the disease (P =0.012,OR =4.758),weak ability in handling it (P ＜ 0.001,OR =3.790),bad health condition(P ＜ 0.0001,OR =5.283 ),aggravation of disease(P ＜ 0.001,OR =2.840),bad martial condition (P =0.009,OR =1.805 ),multiple intercurrent diseases (P =0.001,OR =2.051 ),and dissatisfaction of the salary(P ＜0.001,0R=2.362) and tiredness of working(P=0.0054,OR=3.136).ConclusionThe risk factor of somatic patients with the depression and anxiety include family depression history,past incidence of the disease,weak ability in handling it,bad health condition,aggravation of disease,bad martial condition,multiple intercurrent diseases,and dissatisfaction of the salary and tiredness of working.

Objective To study the reversal effect of tetrandrine (TET) on the multidrug resistance of Hep-2/ADM cell line and the possible mechanisms. Methods MTT method was used for the detection of the inhibitory effect of different concentrations of TET on the multidrug resistance of Hep-2/ADM cell line for selection of the non-toxicity concentration. MTT reduction assay was used to investigate the drug interaction of TET and vincristine (VCR) on HNE-1 (200) cell line. The reversal index (RI), accumulation and excretion of drug, and the apoptosis of Hep-2/ADM cell line were detected by flow cytometry. Results No toxicity to Hep-2/ADM cell line was found when TET concentration was lower than 1.5 ?g/ml. After interaction with TET (1.0 ?g/ml and 1.5 ?g/ml), RIs of VCR on Hep-2/ADM were 1.72 and 2.00 folds, respectively. TET at the concentration of 1.5 ?g/ml could increase the drug excretion and accumulation and also the apoptotic rate of VCR-induced Hep-2/ADM cell line. Conclusion TET can reverse the multidrug resistance of Hep-2/ADM cell line. The reversal effect may be related to the concentrations of TET. The possible mechanism may be that TET can increase the cell apoptosis of VCR-induced Hep-2/ADM cell line and can also increase the drug excretion and accumulation of the multidrug resistance cell line.

Objective To investigate the effect of HIF-2a silencing by transfection of siRNA into MG-63 cells un-der hypoxia. Methods HIF-2αexpression level in MG-63 cells under hypoxia was determined by Western Blot. Small in-terfering RNA (siRNA) was used to construct MG-63/siHIF-2α(siHIF-2α)cells and control MG-63/scramble (NC) cells. The expression levels of HIF-2α, Vascular endothelial growth factor (VEGF), p-Erk/ErK and Mcl-1 in MG-63, NC and si-HIF-2αcells was determined by Western Blot. NC and siHIF-2αcells were cultured under hypoxia. Cell viability was as-sessed by MTT assay. Migration was identified by scratch migration assay. Tumor formation was identified by clone formation assay. Nude mouse subcutaneous xenograft model was used to investigate tumor development in vivo. Results Hypoxia im-proved HIF-2αexpression in MG-63 cells in a time-dependent manner (F=2 037.412,P<0.001). HIF-2αexpression un-der hypoxia in siHIF-2αcells was lower than that in NC cells (P<0.01). Cell viability of siHIF-2αcells under hypoxia for 12 h and 24 h were lower than that in NC cells (P<0.05 or P<0.01). The relative width of scratch in siHIF-2αgroup under hypoxia for 12 h and 24 h were larger than that in NC group (P<0.01 or P<0.01). When cell counts reach 1 000-5 000, the clone formation rates of siHIF-2αcells were lower than that in NC cells (P<0.05 or P<0.01). The expression of VEGF, p-Erk/Erk and Mcl-1 protein under hypoxia in siHIF-2αcells was lower than that in NC cells(P<0.01). Tumor sizes, weights and density of siHIF-2α group in nude mice were suppressed compared with those in NC group (P<0.01). Conclusion Blocking HIF-2αsignal pathway warrants its investigation as a potential strategy in osteosarcoma treatment.

This study was aimed to observe the effect ofJia-Shen prescription (JSP) on angiotensinⅡ (AngⅡ) inhibition, ventricular remodeling in myocardial infarction (MI) rat model. The anterior descending coronary artery of Sprague-Dawley rat was ligated to establish the MI rat model. Rats were randomly divided into the 3 g JSP group, 6 g JSP group, losartan group, model group, and the sham-operation group. Intragastric administration of medication was given 24 h after MI. In the 3 g and 6 g JSP group, JSP was given at the dose of 3 g·kg-1·day-1 and 6 g·kg-1day-1, respectively. Losartan was given at the dose of 10 mg·kg-1·day-1 in the losartan group. Same volume of distilled water was given to the sham-operation and model group. Four weeks later, the left ventricular end-systolic diameter (LVESD), left ventricular end-diastolic diameter (LVEDD), posterior wall thickness (PWT), left ventricular ejection fraction (LVEF), left ventricular fractional shorten (LVFS), left ventricular weight index (LVWI), the distribution and content of collagen, plasma brain natriuretic peptide (BNP) and the AngⅡ content in myocardial tissues homogenate were observed. The results showed that 4 weeks after MI, compared to the model group, 6 g PJP reduced myocardial infarct size, LVWI, LVEDD and LVESD, and enhanced LVEF and LVFS (P< 0.05). In ischemic regions, compared to the model group, JSP can obviously reduce the content of collagen (P < 0.05). This effect had dose-dependent relationship. Plasma BNP and AngⅡ content in myocardial tissues homogenate were also obviously lower than the model group (P< 0.05). It was concluded that JSP can improve the ventricular remodeling of MI rat model. Its action mechanism may be through the AngⅡ inhibition, in order to improve the early stage left ventricular morphological remodeling, myocardial fibrosis and cardiac contractile function.

Objective To evaluate the relationship between the risk of breast cancer and abortions among nulliparous women. Methods Searched the data of Cochrane Library and PubMed before June 2014 to identify potentially studies which involved the relationship between the risk of breast cancer and abortions among nulliparous women. Data was extracted by two independent authors from each study. STATA software was used for statistical analysis. Calculated the pooled RR and 95% CI as the assessment of the link between abortions and breast cancer in fixed effects models. Results 13 studies were included. The study showed the RR and 95%CI of the relationship between the risk of breast cancer and abortions was 0. 98[0. 89,1. 08],P>0. 05 in nulliparous women, and the number of abortions was not associated with the risk of breast cancer. The RR and 95%CI of the relationship between the risk of breast cancer and induced abortions or spontaneous abor-tions were 0. 96[0. 88,1. 04],1. 01[0. 88,1. 14], respectively. Conclusion There is no correlation between breast cancer and abortions a-mong the nulliparous women, and the risk of breast cancer would not increas as the number of abortions increase.

This article was aimed to determine effects of Jia-Shen prescription (JSP) on infarct size (IS), cardiac function and myocardial cytokine in the early phase of myocardial infarction (MI) in rats. Acute MI models were induced by the ligation of left anterior descending coronary artery in Sprague-Dawley (SD) rats. The rats were ran-domly divided into five groups, which were the sham-operated group, model group, JSP-3 g (3 g·kg-1·day-1) group, JSP-6 g (6 g·kg-1·day-1) group, and the losartan (10 mg·kg-1·day-1) group. IS was determined by Evans blue and 2,3,5-Triphenyltetrazolium chloride (TTC) 3 days after MI. The left ventricular structure and contractility were measured by echocardiography performed 7 days after MI. And contents of myocardial inflammatory mediators in-cluding tumor necrosis factor-α(TNF-α), interleukin-1β(IL-1β), and monocyte chemoattractant protein-1 (MCP-1) were measured by ELISA. The results showed that compared with the model group, treatment with JSP at the dose of 6 g significantly reduced myocardial IS (P<0.05);left ventricular end diastolic diameter (LVEDD) and left ventricu-lar end systolic diameter (LVESD) were significantly decreased (P<0.05); left ventricular ejection fraction (LVEF) and left ventricular fraction shortening (LVFS) were significantly increased (P<0.05).The results were similar as the losartan group. Compared with the model group, JSP can significantly reduce the production of TNF-α, IL-1βand MCP-1 in cardiac tissues (P<0.05). Except TNF-α, these effects of JSP were in a dose-dependent manner. JSP (6 g) had equal effectiveness with losartan. It was concluded that consistent with losartan-induced cardioprotection, JSP administered after MI reduced myocardial IS, improved cardiac function, and decreased inflammatory mediators in ischemic myocardium. The data indicated that JSP exerted its cardioprotection possibly via inhibiting inflammatory response.

This study was aimed to explore the method to improve the purity of primary myocardial cells from neonatal rats. The myocardial tissues of rats which were born 1-3 days were repeated digested by 0.08% myocardial cells digestive juices. And then, cells were neutralized with DMEM medium which containing 10% fetal bovine serum. The cells were separated with differential sidewall ion. The red blood cell cracking liquid was added to remove the red blood cells. Bromine deoxidization uracil (Brdu) was added to purify the myocardial cells. Then, cells were incubated in the CO2 incubator for two days. The serum-free synchronization was performed for one day. The results showed that the output of myocardial cells from one rat was about 1.2 × 106. The dynamic myocardial cells occupied more than 90%. The purity of myocardial cells was more than 90%. After 3 to 4 days, the cell fusion of myocardial cells was formed with spontaneous rhythm beats. It was concluded that the method can ensure the yield and the activity of the myocardial cells. At the same time, the purity of myocardial cells can also be improved greatly.

Objective To evaluate the effect of alcohol consumption on bone mineral density in males .Methods Association be-tween bone mineral density ,alkaline phosphatase and alcohol history ,daily alcohol consumption were analysed retrospectively in 563 men undergone physical examination .Results Of all the participates ,31 .6% has history of alcohol consumption for more than one year .The rate of osteopenia and osteoporosis was 31 .4% and 5 .9% ,respectively .The reduction of bone mineral density was posi-tively correlated with age ,length of alcohol consumption history ,daily alcohol consumption volume and the serum alkaline phospha-tase levels(P<0 .01) .The rate of osteopenia and osteoporosis were increased significantly in the males who had alcohol consump-tion history(P<0 .01) ,and paralleled with the increase of daily alcohol consumption (P<0 .01) .The alkaline phosphatase level of males with a history of alcohol consumption more than 20 years were higher than the ones without history of alcohol intake .While alkaline phosphatase level of the ones taking daily alcohol more than 50 g was higher than less than 50g or no consumption signifi-cantly(P<0 .05) .Conclusion Alcohol consumption is an important risk factor of male osteoporosis .

Objective To investigate the relationship between body mass index (BMI) and coronary artery calcification in order to provide theoretical and clinical basis for the prevention and treatment of coronary artery calcification.Methods Ninety hundred and eighty-three cases were selected as our subjects who were hospitalized in the First Affiliated Hospital of Henan University of Traditional Chinese Medicine from Jan.2010 to Jul.2010 and undergone dual source CT coronary angiography.Of them,419 cases were male(male group),and 564 female (female group).The general information,clinical and biochemical indexes and coronary CTA results were collected.The patients were divided according to the BML Multivariate logistic regression analysis was applied to analyzed the relationship between BMI and coronary artery calcification,and multiple linear regression analysis was applied to analyzed the relationship between coronary artery calcification and BMI.Results There were significant differences between male group and female group in terms of age,height,body mass,BMI,smoking history,glomerular filtration rate (eGFR),triglyceride (TG),high density lipoprotein (HDL-C),left ventricular ejection fraction (LVEF),serum calcium,with peripheral vascular disease,as well as the baseline drugs,angiotensin converting enzyme inhibitors or angiotensin receptor blockers (ACEI/ARB),calcium antagonists (CCB),statins compared the differences were statistically significant (P ＜ 0.05).The rate of slight coronary artery calcification in male group and female groups were not statistically significant(x2 =0.714,P =0.398),while the rate of no calcification,severe calcification were statistically significant (P ＜ 0.05).Multivariate Logistic regression analysis showed that high BMI(regression coefficient was-1.670,OR =0.967,95％ CI =0.953 ～ 0.980,P =0.005),age (regression coefficient was 1.422,OR =4.416,95％ CI:1.015 ～ 16.927,P =0.001),history of hypertension (regression coefficient was 0.128,OR =1.521,95％ CI:1.262 ～ 1.830,P =0.002),history of diabetes mellitus (regression coefficient was 0.364,OR =1.439,95 ％ CI:1.098 ～ 1.885,P =0.008),eGFR (regression coefficient was-0.5420,OR =0.004,95％ CI:0.001-0.019,P =0.014),LVEF (regression coefficient was-1.153,OR =0.316,95％ CI:0.127-0.787,P =0.002) and statins(regression coefficient was-6.745,OR:0.323,95％ CI:0.138-0.754,P =0.032) were correlated with coronary artery calcification.Multiple stepwise linear regression analysis showed that only eGFR(r =0.79,95％ CI:0.69-0.92,P =0.001) was in the equation.Conclusion High BMI is a protective factor for severe coronary artery calcification,but there is on linear correlation between BMI and moderate to severe coronary artery calcification score in patients.