1.Preparation and molecular weight estimation of ouabain pol yclonal antibody F(ab)2 fragment
Mingjuan ZHANG ; Zhuoren LU ; Yukang YUAN ; Langchong HE ; Hao WANG
Chinese Journal of Biochemical Pharmaceutics 2001;22(1):5-7
Purpose The aim is to prepare ouabain polyclonal antibody F(ab)2 fragment and to estimate its molecular weight.Methods[ KG*2 [ WTBZ]Ouabain polyclonal antibody was obtained from immunized rabbits.The antibod y was digested with pepsin.The resulting products were analyzed and the molecular weig ht of F(ab)2 fragment was estimated by HPSEC.The immune activity was detec ted by ELISA.Results 100 mg of ouabain polyclonal antibody wa s dige sted by 2 mg of pepsin for 18 hours at pH 3.0 and active ouabain polyclonal anti body F(ab)2 framgment was obtained.Its molecular weight was 107 kD.Concl usion The active ouabain polyclonal antibody F(ab)2 fragment coul d be prepared by digesting its antibody with pepsin.
2.Isoform-specific regulation of sodium pump ?-subunit gene expression in the cortex of kidney from the one kidney one clip hypertensive rat
Weiqing YUAN ; Hao WANG ; Zhuoren LU ; Mingjuan ZHAN ; Ju YANG
Chinese Journal of Pathophysiology 1986;0(01):-
AIM: To investigate the regulation of sodium pump ?-subunit gene expression in the cortex of kidneys from one kidney one clip (1k1c) hypertensive rats. METHODS: 1k1c hypertensive rats were prepared by partially ligating the left renal artery and removing the right kidney. 4 weeks later, all the rats were killed, the levels of sodium pump ? 1-, ? 2-, and ? 3-subunit mRNA and protein in the cortex of kidney were detected with reverse transcription polymerase chain reaction (RT-PCR) method and immunohistochemical assay, respectively. RESULTS: The mRNA levels of sodium pump ? 1-subunit increased and ? 2- and ? 3-subunit were unchanged in the cortex of kidneys from 1k1c hypertensive rats compared with control rats, while no change has been found for all the three subunits gene expressions at protein level. CONCLUSION: There were some changes in the expression of sodium pump ?-subunit gene in the cortex of kidney of the 1k1c hypertensive rat, which might be related to the development of hypertension in this hypertensive model.
3.Extracorooreal membrane oxvgenation treatment of a child with fulminant myocarditis
Jingbo YUAN ; Xianmei HUANG ; Changwen LIU ; Mingjuan DAI
Chinese Pediatric Emergency Medicine 2011;18(5):423-426
Objective To report the clinical experience of extracorporeal membrane oxygenation (ECMO)in the emergency management of fulminant myocarditis.Methods The patient,an 11 year-old boy,had fever for 4 day and abdominal pain,vomiting for 1 day and anuria for 12 hours.Electrocardiogram showed Ⅲ atrial-ventricular block,multifocal ventricular rhythm,bundle branch block,convulsivum multifocal ventricular tachycardia,extensive low voltage,ST-T elevation at lead Ⅰ,AVR,V1,V2,V3;and there were ST-T intrusion,T wave inversion at lead Ⅱ,Ⅲ,AVR,AVF,V4~5.Blood creatine kinase was 2 161 U/L,reatine kinase isoenzyme 109 U/L,α-hydroxybutyrate dehydrogenase 612 U/L,lactate dehydrogenase 696 U/L,troponin Ⅰ 22.1 U/L.Echocardiogram showed right atrium 4.4 mm,right ventricular 2.3 mm,severe tricuspid valve regurgitation,left ejection fraction 33%,left ventricular fractional shortening 15%,ventricular wall motion dyssynchrony.Blood lactate was 4.0 mmol/L.The patient's condition was still unstable after using dobutamin,dopamine,milrinone,furosemide,large dose methylprednisolone,intravenous human immunoglobin,phosphocreatine and so on.ECMO was used for cardio-pulmonary support.It is necessary to monitoring the consciousness,temperature,heart rate,respiration,blood pression,SaO2,urinary volume,ariterial blood gas,blood electrolytes,blood lactate,blood glucose,liver function,renal function,blood routine,activated clotting time(ACT),lower extremity blood supply and so on.ACT was maintained at 160~200 s.Heparin was used persistently[5~10 U/(kg·min)].Results ECMO system had been successfully used for 7 days.The cardiac function of the patient was improved significantly.There was no complication,such as hemorrhage,infection,and embolism.Heart arrest in the patient occurred three times,ventricular fibrillation and ventricular flutter occurred one time respectively during ECMO.The rhythm was recovered by electric defibrillation and antiarrhythmic drugs.On day 20,the patient was discharged.At the time of hospital discharge,the patient demonstrated good activity,with normal myocardial enzymes.The echocardiogram showed the size of the cardiac chambers and the contractile function of the myocardia were normal.Electrocardiogram showed Ⅰ degree atrial-ventricular block,complete right bundle branch block.Two weeks later,the electrocardiogram demonstrated complete right bundle branch block.Echocardiogram showed septal thickening(0.9 cm).Two months later,the electrocardiogram was just as that of two weeks before.Echocardiogram showed septal thickening(0.7 cm).The children had no symptom after he was discharged and acted without limitation.Conclusion ECMO is a kind of effective treatment for fulminant myocarditis.The key to desirable therapeutic effect is the timing of its application.
4.Identification of novel transcripts and sRNA of Brucella melitensis by RNA-Seq
Yingfei GUO ; Yufei WANG ; Chunli GONG ; Mingjuan YANG ; Jiuyun YUAN ; Yubing ZHUANG ; Yuehua KE ; Xinying DU ; Zhoujia WANG ; Zeliang CHEN
Chinese Journal of Zoonoses 2015;(3):216-221
To identify novel transcripts and sRNA in genome of B .melitensis by transcriptome sequencing ,total RNA were extracted from B .melitensis culture and rRNA were removed .After the addition of adaptor ,RNA was reversely transcribed into cDNA ,which were then subjected to PCR amplification and sequencing .The generated reads were mapped to genome se‐quence of B .melitensis strain 16M .With the mapping results ,novel transcripts and sRNA were identified by bioinformatics methods .Sequencing results analysis showed that genome sequence was covered with the reads with good quality .A total of 773 genes were extended in their 5′and/or 3′ends of their original locations .Sixteen novel transcripts and 241 sRNAs candi‐dates were identified .RT‐PCR showed that some of the sRNAs were differentially expressed under stress conditions .In B . melitensis genome ,there is novel transcript which is not predicted .The sRNA does exist in B .melitensis and were expressed under different conditions .
5.Diagnosis and treatment of seven patients with brucellosis in non-pastoral areas
Mingjuan YUAN ; Sihai LI ; Yan HUANG ; Weidong FU
Chinese Journal of Internal Medicine 2019;58(8):596-598
To explore how to diagnose and treat brucellosis accurately and timely in patients with fever of unkown origin in non-pastoral areas.The epidemiological history,clinical symptoms,complete blood counts,procalcitonin and treatment efficacy of 7 patients with brucellosis were analyzed retrospectively.Some characteristic manifestations should be differentiated from tuberculosis.The clinical symptoms were relieved after combination of doxycycline,rifampicin,levofloxacin and amikacin for 6 weeks,only one patient with bone destruction needed orthopedic surgery.The overall response rate was 6/7.No relapse occurred during half year follow-up.
6. Curative effect of dual antiplatelet therapy for non-ST-segment elevation acute coronary syndrome after percutaneous coronary intervention
Chaofeng SHEN ; Mingjuan SHI ; Jindong SUN ; Hong YUAN
Chinese Journal of Primary Medicine and Pharmacy 2018;25(11):1444-1447
Objective:
To observe the effect of ticagrelor for non-ST-segment elevation acute coronary syndrome after percutaneous coronary intervention (PCI).
Methods:
A total of 200 patients with non-ST-segment elevation acute coronary syndrome after PCI were enrolled in this study.And the patients were randomly divided into the observation group (18 months treatment group, 100 cases) and control group (12 months treatment group, 100 cases) according to the digital table.The control group was given dual antiplatelet therapy (DAPT) for 12 months, and then, suspended the usage of ticagrelor.The observation group was treated by DAPT for 18 months.The major adverse cardiovascular events (MACCE) and the secondary end point events were observed.
Results:
The incidence rate of MACCE between the two groups had no statistically significant difference (χ2=0.298,