Digital CR of head and neck overcomes the disadvantage of regular X-ray radiography, which can not reveal bone and soft tissue position deficiency in one exposing, and reduces the X-ray radiation dose. Meanwhile, various factors decline the imaging quality, and images must be enhanced in order to meet demands of doctors clinical diagnosis. The general enhancement algorithms don't consider bodys structure difference and density characteristics. A new adaptive CR enhancement algorithms was proposed in this article, and head and neck images were processed with this method and compared with linear unsharp masking method. The results proved that the details of CR image enhanced were abundant and enhanced CR had good visual effect. SNR was high, as well as detail variance/background variance (DV/BV) indicating that this algorithms is suitable for head and neck CR medical image.

Circulating tumor cells (CTCs) are essential for establishing metastasis. Detecting CTCs can help clinicians detect early metastases or recurrences more effectively. Compared with traditional histological analyses and imaging, CTC detection has a much higher reproducibility and sensitivity and can provide more timely prognostic information in human breast cancer.

Objective To investigate the Effect of Tetramethylpyrazine on adhesion and invasion ability of Cell PG in Hypoxic Environment. Methods The revival cell PG was divided into three dosages(high, medium and low)group of Tetramethylpyrazine, DDP group and negative control group. All of five groups were cultured separately in the normal and hypoxic environment. The adhesion ability of Cell PG were detected by MTT. The invasion ability of Cell PG were detected by Transwell chamber. Results Observed on the reconstruction of the basement membrane adhesion in PG cells by MTT, in normoxic environment the mean absorbance of the TMP high, medium and low concentrations, cisplatin group, control group were (0.556±0.040),(0.519±0.056), (0.517±0.061),(0.333±0.057),(0.300±0.066), P<0.01. In hypoxic environment the mean absorbance of the TMP high , medium and low concentrations, cisplatin group, control group were (0.48±0.032),(0.452±0.059),(0.274±0.093),(0.464±0.099),(0.155±0.042), P<0.01. Promotion of cell adhesion with Tetramethylpyrazine of high, medium and low concentration group and DDP group in hypoxic environment is greater than in normoxic environment(P<0.01). Observed the reconstruction of the invasion of basement membrane of PG cells by Transwell chamber, in normoxic environment the invasive cells of the TMP high, medium and low concentrations, cisplatin group, control group were :(28.00±17.00),(91.67±30.37),(105.67±39.58),(85.00±8.19),(152.33±24.71), P<0.05. In hypoxic environment the invasive cells of the TMP high , medium and low concentrations , cisplatin group, control group were (20.33±15.31), (22.33±17.62),(56.00±11.53),(57.67±35.95), (76.33±18.01), P<0.05. The invasive cells of normoxic control group was significantly more than hypoxic control group(P<0.05). Conclusion In hypoxic environment , adhesion and invasion ability of Cell PG significantly decreased compared with normoxic environment ; In normoxia and hypoxia environment, Tetramethylpyrazine and cisplatin could promote the adhesion ability and inhibit the invasion ability of PG cells. Role in promoting of Tetramethylpyrazine on cell adhesion of PG cells was stronger in hypoxic environment.

OBJECTIVETo evaluate the short effect and safety of the acupoint application of dog days for allergic rhinitis.

METHODSTwo hundred and forty-nine patients were randomly divided into an application group (166 cases) and a placebo group (83 cases). On the first day of the first dog days, the first day of the second of the three ten-day periods of the hot season and the first day of the last of the three ten-day periods of the hot season, acupoint application was adopted mainly at Dazhui (GV 14), Feishu (BL 13), Pishu (BL 20) and Shenshu (BL 23) in the application group. The plaster was made of Chinese herbs, including Baijiezi (semen brassicae), Xixin (asarum), Yanhusuo (corydalis tuber) and Gansui (euphorbia kansui). Buckwheat plaster without medical ingredient was used in the placebo group. The application was pasted for 2-6 h every time. On the first three days of all the three periods of dog days, treatment was used continuously. The whole period of dog days was made into one course. The changes of symptom and syndrome scores, visual analogue scale (VAS) for symptom before and after treatment and the adverse reaction with the condition disposed were observed in the two groups.

RESULTSAfter treatment, the symptom and syndrome scores and VAS for symptom were improved compared with those before treatment in the two groups (all P < 0.05), and the improvements of the application group were more obvious than those of the placebo group (all P < 0.05). The short effective rate of the application group was 68.1% (113/166), which was better than 48.2% (40/83) of the placebo group (P < 0.05). The adverse reaction rates were 4.2% (7/166), 2.4% (2/83) respectively, with no statistical significance between the two groups (P > 0.05).

CONCLUSIONThe dog days plaster achieves obvious effect with good safety for allergic rhinitis.

Acupuncture Points ; Adolescent ; Adult ; Drugs, Chinese Herbal ; administration & dosage ; adverse effects ; Female ; Humans ; Male ; Middle Aged ; Rhinitis, Allergic ; drug therapy ; Treatment Outcome ; Young Adult

Objective To study the effect of p50 on IKKα at IL-1β promoter in LPS tolerant cells and to reveal the mechanism of the inhibition of IL-1β mRNA by pS0. Methods THP-1 human promono-cyte model of endotoxin tolerance that simulates the sepsis leukocyte phenotype was used. Chromatin immu-noprecipitation assay(CHIP) and real-time PCR were applied to quantify the binding of p50 and IKKα to IL-1βpromoter. IL-1β mRNA transcription was studied after knocking-down of p50 and/or IKKα. Results With LPS stimulation, p50 binding did not reduce but somewhat increased at IL-1β promoter in tolerant THP-1 cells. Knocking-down of p50 increased the transcription of IL-1β mRNA, which revealed the inhibi-tory effect of p50 in tolerant cells. In contrast, the accumulation of IKKα to IL-1β promoter decreased with LPS stimulation in tolerant cells; However, IKKα binding increased after p50 gene knock-down. In the meantime, IL-1β mRNA transcription increased; At last, IL-1β mRNA decreased again after double-knoc-king down of p50 and IKKα. Conclusion p50 is an inhibitory protein at IL-1β promoter in tolerant THP-1 cells. The unresponsiveness of IL-1β mRNA transcription to LPS at least partly results from the inhibition of IKKα binding to IL-1β promoter by p50.

Objective To evaluate the clinical effectiveness of combined use of traditional Chinese and Western medicine for chronic pelvic inflammatory diseases.Methods By searching in the major databases of CNKI,ISI Web of Knowledge,VIP Information and Pubmed,we collected data of randomized controlled trials pertaining to combined use of traditional Chinese and Western medicine in the treatment of chronic pelvic inflammatory diseases.Results Twenty-five randomized controlled trials involving totally 3081 patients were collected according to the inclusion criteria,and meta-analysis of the data indicated that combined use of traditional Chinese and Western medicine can be of great value in the management of chronic pelvic inflammatory diseases in comparison with exclusive use of Western medicine [combined odds ratio(OR)was 3.20 with 95% confidence interval(CI)(2.74,3.73),Z=14.85,P＜0.01].Conclusion The clinical evidence derived from the analysis suggests that the combination of traditional Chinese and Western medicine for chronic pelvic inflammatory diseases can be effective with good security.

A main complication of chemotherapy in cancer patients is hematopoiesis suppression. Microenviroment transplantation using bone marrow stromal cells (BMSCs) has been demonstrated to be a potent method in recovery of hematopoiesis in animal models. Based on hematopoiesis-supportive ability of BMSCs and high potency of IL-3 in hematopoiesis stimulation, BMSCs were studied as a cellular delivery system for IL-3 gene transfection to promote hematopoiesis recovery of mice after high dose chemotherapy. BMSCs were transfected with recombinant adenovirous containing murine IL-3 gene(MOI = 10), the level of mIL-3 secreted by gene-modified BMSCs was 110U/ml/10~6 cells/ 24h in vitro. The mice were injected with high dose cyclophosphamide(200mg/kg) i.p. and after 24 hours the IL-3 gene-modified BMSCs(2 x 10~6/mouse) were transplanted intrasplenically. White blood cell counts in peripheral blood of mice received intrasplenic injection of IL-3-BMSCs were kept at a high level within two weeks after chemotherapy. The pathological sections of spleens and bone marrow showed significant recovery of hematopoiesis, compared with that of mice received chemotherapy only. The data indicated the feasibility of IL-3 gene-modified BMSCs transplantation in the acceleration of hematopoiesis recovery after chemotherapy.

Dendritic cells (DC) are important antigen-presenting cells in the development of anti-leukemic T-cells responses and it' s reported that DC can differentiate from monocytes or granulocytes in vitro. In the present study, the human leukemic DC were generated from acute promyelocytic leukemic( APL)cells after treatment with cytokines. M3 APL cells were isolated from peripheral blood of patients and incubated with rhGM-CSF( 100ng/ml)or rhGM-CSF( lOOng/ml) plus rhIL-4(500U/ml)for 14 days and the cells were co-cultured wirh TNF-?(100ng/ml)during last 3 days. The results demonstrated that both proliferation and differentiation of APL cells were induced by GM-CSF as the number of cultured cells increased and the cells expressed high level of CD45. Some cells displayed the characteristics of monocytes which expressed CD14 and some were leukemic DC as they expressed GDI a. The co-culturation of APL cells with GM-CSF and TNF-? augmented the differentiation of cells and about 35 percent of leukemic DC generated (was obtained). The maturation of APL cells could also be induced by GM-CSF plus IL-4 but there were few monocytes generated and about 10 percent of the cells were leukemic DC. If cultured for 3 weeks, the number of leukemic DC increased to 60 percent. The addition of TNF-? could augmented GM-CSF and IL-4 induced maturation of cells significantly and 90 percent of leukemic DC generated. Analysis of the cells with electric microscopy indicated that these leukemic DC displayed the similarly morphologic characteristics as monocyte-derived DC and there were still a few granules in their cytoplasm. The leukemic DC expressed high levels of HLA-DR, B7-1, B7-2 and CD54 molecules and could stimulate allo-T cells to proliferate in vitro. Such kind of leukemic DC might be useful tools for the generation of specific anti-tumor CTL and play important roles in immunotherapy of APL.

Objective: To investigate the effects of DC derived from IL-12-induced erythroleukemia cells on the induction of CTL and protective antituinor immunity. Methods: The cytotoxicity of CTL was assessed by 4h 51 Cr-release assay. HE staining and eleclromicroscopy were used to observe the histological changes after immunized with erythroleukemia-derived DCs. Results: After incubation with naive T cells, the IL-12-induced FBL-3 cells could apparently induce the generation of CTL which exihibit the specific cytotoxicity against wild-type FBL-3 cells in vitro. We further assayed the cytotoxicity of CTL in vivo after immunized with erythroleukemia-derived DCs. CTL cytotoxicity of mice immunized with IL-12-in-duced FBL-3 cells was higher than that of mice immunized with IL-I2-uninduced FBL-3 cells. C57BL/6 mice immunized with IL-12-induced FBL-3 cells could resistant the subsequent rechallenge with the wild-type FBL-3 cells. The tumor growth was efficiently inhibited. Histological observation showed that more inflammatory cells infiltrated into the tumor tissue and necrosis of leukemia cells existed. By transmission electron microscopy, apoptositic phenomena were observed in the tumor of group immunized with IL-12-induced FBL-3 cells. However, these changes were not observed in the group immunized with IL-12-uninduced FBL-3 cells. Conclusion: These data indicate that erythroleukemia cells-derived DCs could induce specific CTL efficiently in vitro and in vivo, and may be used as new vaccine to activate antituinor immune responses.

Objective To compare the effects of laparoscopic hepatectomy (LH) versus conventional laparotomy hepatectomy (CLH) on cellular immunity. Methods Fifteen ASA Ⅱ-Ⅲ patients aged 34-61 yrs, weighing 48-75 kg undergoing laparoscopic hepatectomy (LH) were studied. Another 15 patients aged 33-64 yrs, weighing 46-73 kg undergoing conventional laparotomy hepatectomy (CLH) served as control. The preoperative liver function was rated as Child classification A in both groups. The patients were premedicated with phenobarbital 0.1 g and atropine 0.5 mg i.m. . Anesthesia was induced with fenlanyl 4 ?g?kg-1, propofol 1.5 mg?kg-1 and succinylcholine 2 mg?kg-1. After tracheal intubation the patients were mechanically ventilated and PETCO2 was maintained at 35-45 mm Hg. Anesthesia was maintained with inhalation of isoflurane (MAC 1.0?0.31) and 60% N2O in O2 and intermittent i.v. boluses of vecuronium. The patients received after operation patient-controlled epidural analgesia (PCEA) with 0.125% ropivacaine and morphine 0.05 ?g?kg-1?min-1. Radial artery and right internal jugular vein were cannulated for BP and CVP monitoring. Peripheral venous blood samples were taken before operation and on the 1 st and 3rd postoperative day for determination of CD3+ , CD4+ , CD8+ T cells (by flow cytometry) and IL-6, TNF-?concentrations (ELBA) . Results CD3+ , CD4+ and CD8+ counts were significantly decreased while IL-6 and TNF-?levels were significantly increased on the 1st postoperative day compared with the baseline values before operation in both groups but there was no significant difference between the two groups. On the 3rd postoperative day CD3+ , CD4+ and CD8+ counts and IL-6, TNF-?levels returned to preoperative level in group LH while in group CLH CD3+ , CD4+ , CD8+ remained low and IL-6, TNF-?levels remained high.Conclusion The results suggest that LH exerts less effects on immune function than conventional laparotomy technique.