Outlining the medicinal properties of unprocessed Rhizoma Pinelliae and its general usage nowadays,this paper highlighs the view of veteran TCM practitioner LI Ke on processed Rhizoma Pinelliae and the reasons for the clinical overdose application and its clinical effect.Besides,before proposing a new thinking to the clinical use of herbs in the future,the author concludes his clinical experience in application of unprocessed Rhizoma Pinelliae under the guidance of Master LI,particularly the application in the treatment of difficulty miscellaneous disease.

AIM： To investigate the different expressions of cell cycle related genes in hyperplastic and hypertrophic vascular smooth muscle cells caused by platelet-derived growth factor (PDGF-BB) and angiotensinⅡ(AngⅡ). METHODS: Rat aorta media smooth muscle cells were cultured. PDGF-BB and AngⅡ were added into serum-free medium at a concentration of 20 μg/L and 10-6 mol/L , respectively. Vascular smooth muscle cells (VSMCs) were harvested after stimulated for 24 hours. The expression of cell cycle related genes was measured by DNA chips(Atlas cDNA Expression Arrays, Clontech Laboratories, Inc.). RESULTS: The expression of cyclin D3 mRNA ，cyclin G1 mRNA，p57 mRNA，p16 mRNA，E2F-3 mRNA and DP2 mRNA were higher in PDGF-BB than those in AngⅡstimulated VSMCs. p15 mRNA，p19 mRNA，E2F-1 mRNA， E2F-5mRNA,and N-myc mRNA were only detected in PDGF-BB stimulated group. But the expression of p53-associated protein mRNA were higher in AngⅡstimulation group. The expression of PCNA mRNA, c-myc binding protein mRNA，p53-dependent cell growth regulater mRNA，cyclinC mRNA, cyclinB1 mRNA, E2F-3mRNA were similar in the two groups. CONCLUSION: The procession of cell cycle relys on the coordination of many regulater molecules expressed in different phases. Our study preliminarily definite the genes that express during PDGF-stimulated VSMC's hyperplasia and Ang II-stimulated VSMC's hypertrophy.

Objective To compare the efficiency and safety of intracoronary transplantation of peripheral blood stem cells (PBSC) between elderly and younger patients with heart failure after myocardial infarction (MI). Methods Twenty-five patients with heart failure after MI were divided into aged group(≥60 years,n=13) and non-aged group (＜60years,n=12) to receive intracoronary PBSC transplantation (PBSCT) following bone marrow cells mobilized by granulocyte colony-stimulating factor (G-CSF). Clinical data including coronary lesion characteristic, left ventricular shape, infarct region area and cardiac function, as well as adverse side effects between the two groups were compared. Left ventricular function was evaluated before and 6 months after the treatment by single photon emission computed tomography (SPECT). Results At 6 months, the left ventricular ejection fraction (LVEF) and 6 minute walk test (6MWT)distance increased, while the left ventricular diastolic diameter (LVDd) decreased significantly in both groups. There were no significant difference between the two groups in absolute change in the cardiac function parameters. Conclusions The present study demonstrated that autologous intracoronary PBSCT might be safe and feasible for both old and younger patients with heart failure after MI and left ventricular function is significantly improved.

Objective To investigate the Toxoplasma gondii infection status in pregnant women with history of adverse preg?nancy and risk factors in Bazhou area,Hebei Province. Methods A total of 302 pregnant women with the history of adverse pregnancy were chosen as respondents(an experimental group)in the hospital from March 2012 to December 2015,and 197 pregnant women without the history of adverse pregnancy as a control group. TOX?IgG and TOX?IgM were detected by using ELI?SA in two groups. The risk factors of Toxoplasma infection were surveyed by questionnaires. Results The total positive rate of Toxoplasma antibodies was 28.15%(85/302)in the experimental group,which was significantly higher than that[9.64%(19/197)]in the control group,and the difference was statistically significant(χ2=24.76,P<0.05). The positive rates of TOX?IgM,TOX?IgG and TOX?IgM+TOX?IgG were 6.95%(21/302),18.54%(56/302),and 2.65%(8/302)respectively in the ex?perimental group,which were higher than 2.03%(4/197),7.61%(15/197),and 0%(0/197)respectively in the control group (χ2=6.07,11.67,3.76,all P<0.05). The questionnaire survey showed that the proportions of keeping pets,cutting board re?gardless,liking to eat hot pot or barbecue,eating raw meat,often eating in the restaurant in the pregnant women with Toxoplas?ma infection were higher than those in the pregnant women without Toxoplasma infection,and the differences were statistically significant(χ2=22.57,3.96,5.87,7.40,4.86,all P<0.05),and therefore,the above unhealthy habits may be important risk factors. Conclusions Toxoplasma infection could lead to adverse pregnancy outcomes. Therefore,the above?mentioned unhealthy habits should be avoided,especially during pregnancy period.

Objective: To compare the different therapeutic effects of acupuncture and Phenolphthalein for constipation due to intestinal qi stagnation. Methods:A total of 50 patients with constipation due to intestinal qi stagnation were randomly divided into an acupuncture group and a medication group by the random digital table, 25 cases in each group. The patients in the acupuncture group were treated by puncturing Gongsun (SP 4), Sanyinjiao (SP 6), Taichong (LR 3), Zusanli (ST 36), Shangjuxu (ST 37), Hegu (LI 4), Lieque (LU 7), and Tianshu (ST 25), once every day, and 7 d as one course, for continuous 3 courses; while the patients in the medication group were given Phenolphthalein, 7 d as one course, for continuous 3 courses. Compared the Cleveland clinic constipation score (CCS) between the two groups after one course, 3 courses and 3 months after the treatment, as well as the frequency of defecation within one week. Results:After one week of treatment, CCS scores and frequency of defecation per week were significantly changed in both groups compared with those before treatment (P<0.05), and CCS scores and frequency of defecation per week were improved more significantly in the patients of the medication group than in those of the acupuncture group (P<0.01). After 3 weeks of treatment, CCS scores and frequency of defecation per week were significantly changed in both groups compared with those before treatment (P<0.05), and CCS scores and frequency of defecation per week were improved more significant in the patients of the acupuncture group than in those of the medication group (P<0.05). Three months after the end of treatment, CCS scores and frequency of defecation per week were significantly changed in both groups compared with those before treatment (P<0.05), and CCS scores and frequency of defecation per week were improved more significantly in the patients of the acupuncture group than in those of the medication group (P<0.05). Conclusion:Acupuncture and medication are effective in the treatment of constipation due to intestinal qi stagnation. Their short-term therapeutic effect is similar, but the long-term therapeutic effect is better in the acupuncture group than in the medication group.

AIM: To investigate the different expressions of cell cycle related genes in hyperplastic and hypertrophic vascular smooth muscle cells caused by platelet-derived growth factor (PDGF-BB) and angiotensinⅡ(AngⅡ). METHODS: Rat aorta media smooth muscle cells were cultured. PDGF-BB and AngⅡ were added into serum-free medium at a concentration of 20 ?g/L and 10 -6 mol/L , respectively. Vascular smooth muscle cells (VSMCs) were harvested after stimulated for 24 hours. The expression of cell cycle related genes was measured by DNA chips(Atlas cDNA Expression Arrays, Clontech Laboratories, Inc.). RESULTS: The expression of cyclin D3 mRNA ,cyclin G1 mRNA,p57 mRNA,p16 mRNA,E2F-3 mRNA and DP2 mRNA were higher in PDGF-BB than those in AngⅡstimulated VSMCs. p15 mRNA,p19 mRNA,E2F-1 mRNA, E2F-5mRNA,and N-myc mRNA were only detected in PDGF-BB stimulated group. But the expression of p53-associated protein mRNA were higher in AngⅡstimulation group. The expression of PCNA mRNA, c-myc binding protein mRNA,p53-dependent cell growth regulater mRNA,cyclinC mRNA, cyclinB1 mRNA, E2F-3mRNA were similar in the two groups. CONCLUSION: The procession of cell cycle relys on the coordination of many regulater molecules expressed in different phases. Our study preliminarily definite the genes that express during PDGF-stimulated VSMC's hyperplasia and Ang II-stimulated VSMC's hypertrophy. [

Objective To evaluate the prophylactic efficacy and safety of posaconazole against invasive fungal disease(IFD)in hematologic patients with neutropenia.Methods Medical records of 18 hematologic patients with neutropenia received posaconazole for preventing IFD in a Beijing hospital between 2014 and 2015,the efficacy and safety was evaluated.Results There was no clinical diagnosis or confirmed diagnosis of IFD among 18 patients during posaconazole prophylaxis period,none of patients stopped posaconazole due to severe adverse reaction.Two patients with acute myeloid leukemia(AML) died of pulmonary infection,1 of whom isolated Stenotrophomonas maltophilia from sputum culture on the 12th day of posaconazole prophylaxis,the other isolated Escherichia coli from sputum culture on the 14th day of posaconazole prophylaxis.Other patients all adherence to posaconazole prophylaxis until granulocyte count recovered,patients were followed up until 100 days medication,no death occurred.The lowest peripheral neutrophil count was(0.00-0.27) x 109/L during posaconazole prophylaxis period,with the median of 0.02 x 109/L;the duration of posaconazole prophylaxis was 8-27days,with the median of 16 days;among patients without IFD breakthrough or received systemic use of other antifungal agents,there were 2 (11.1％) all-cause death within 100 days;there were no adverse reaction,such as liver function abnormalities≥grade 2 and kidney function abnormalities≥grade 2,as well as QTc prolongation.Conclusion Posaconazole is effective for preventing IFD in hematologic patients with neutropenia,adverse reaction is rare.

Objective To discuss the application of endoscopic techniques in the treatment of calculus of intrahepatic or extrahepatic duct. Methods Laparoscopy in conjunction with duodenoscopy or cholangioscopy was adopted in 96 cases of calculus of intrahepatic or extrahepatic duct, including 72 cases of cholecystolithiasis complicated by choledocholithiasis, 16 cases of simple choledocholithiasis and 8 cases of left intrahepatic duct calculus complicated by choledocholithiasis. The surgical procedures included laparoscopic cholecystectomy (LC) combined with EST (37 cases), LC combined with cholangioscopic exploration (54 cases), and laparoscopic left hepatic lobectomy combined with cholangioscopic exploration (5 cases). Results Postoperative re-examination revealed no residual calculus in 93 out of 96 patients. Small amounts of biliary leakage occurred in 4 patients and healed spontaneously within a mean time of 10 days. No other severe complications took place. A conversion to open surgery was required in 3 patients, 2 of which were high bile duct stricture and 1 of which were severe portal adhesion. Conclusions Endoscopy in the treatment of calculus of intrahepatic or extrahepatic duct is feasible. Proper application of multiple endoscopic techniques is a safe, effective and minimally invasive means for the treatment of cholelithiasis.

ObjectiveTo study the relationship between vitamin D receptor (VDR) gene polymorphism and calcitonin receptor (CTR) gene polymorphism, and bone mineral density (BMD) of the Han nationality woman in Hebei, explore the pathogenesis of osteoporosis (OP) at the gene level.MethodsPolymorphisms of VDR gene and CTR gene were analyzed by restriction fragment length polymorphisms (RFLPs) in 60 Han nationality women in Hebei.ResultsBb genetype of VDR had lower BMD values at all sites which were measured compared with bb genetype (P<0.05); CC genetype of CTR had tendency for lower BMD values at the L1～L4 compared with CT genetype (0.05

目的观察绝经前后妇女骨密度的变化与甲状旁腺激素、降钙素和1,25(OH)2D3及相关生化指标间的关系。方法采用X线骨密度仪测量骨密度,用放免法检测患者血中甲状旁腺激素、降钙素水平,利用全自动生化仪检测血钙、血磷、血碱性磷酸酶(ALP)。结果绝经后妇女各部位骨密度均下降(P<0.05);血甲状旁腺素水平升高 (P<0.05);降钙素水平有下降趋势;血钙、血磷均无明显变化(P>0.05);血中ALP水平较绝经前升高(P<0.05)。结论妇女绝经后,骨代谢激素分泌发生紊乱,骨密度下降。