ObjectiveTo seek an effective method in treating urinary retention due to incomplete spinal cord injury.Method Forty patients were randomized into two groups. Twenty subjects in the treatment group were intervened by alignedneedling at Jiaji points (EX-B2) plus warm needling; the other 20 subjects in the control group were by aligned needling at Jiaji points (EX-B2) alone. ResultAfter intervention, urinary retention symptoms were improved in both groups (P<0.05), and the improvement in the treatment group was more significant than that in the control group (P<0.05); the totaleffective rate was 85.0% in the treatment group versus 55.0% in the control group, and the difference was statistically significant (P<0.05).ConclusionAligned needling at Jiajiplus warm needling can produce a more significant efficacy than aligned needling at Jiajialone in treating urinary retention after incomplete spinal cord injury.

collagen type Ⅰ.The epithelial surfaceof HABM was significantly better in promoting forebrain cells growth than thestromal surface(P

The incidence of diabetes has been increasing with each passing year and diabetic peripheral vascular disease has been one of the serious complications, which increasingly threatens the numerous sufferers' health. The preoperative imaging evaluation measures for the peripheral vascular disease include Doppler ultrasound (DUS), CT angiography (CTA). MR angiography (MRA). Digital subtractionangiography (DSA). In the situation of today that both the imaging equipments and imaging technology are developing swiftly, it is necessary to make an evaluation of these imaging ways. This paper aims to discuss the clinical value, the limitation and the progresses of the above devices, and to summarize the advantages and the recent advances of preoperative MR angiography used for observing lower limb arterial diseases in diabetic patients. Medical researches have showed that MRA , as a non-invasive method with almost the same high diagnostic accuracy as DSA, is an ideal technique for making preoperative evaluation and long-term postoperative checkup.

Acute ischemic stroke is a common disease serevely threatening the public health. The main management involves intravenous thrombolysis, intra-arterial thrombolysis and mechanical embolectomy. Recently, endovascular mechanical embolectomy is attracted more attention because it provides a good outcome for patients either ineligible not responsible for IV thrombolytic therapy. The article reviews the relative aspects with summerization in this field.

Objective To study the effects of several factors on bud proliferation and rooting of Dioscorea bulbifera stem with a bud.Methods Single factor test and plant tissue culture methods were applied.Results The combination of 6-BA or KT and NAA helped the proliferation of stem with a bud in D.bulbifera;high concentration sucrose led to callus growth of stem with a bud in D.bulbifera which didn′t help the proliferation of stem with a bud in D.bulbifera;Liquid culture also helped the proliferation of stem with a bud in D.bulbifera;In a certain range,the increase of NAA concentration helped the rooting of stem with a bud in D.bulbifera.But it will inhibit the rooting with concentration in a higher level.Conclusion The best proliferation culture medium of D.bulbifera,stem with a bud is MS + KT 2 mg/L +NAA 0.5 mg/L or MS + 6-BA 2 mg/L + NAA 0.5 mg/L;For proliferation of D.bulbifera stem with a bud,the best sucrose concentration is 30 g/L sucrose,the best culture mode is liquid culture;The best rooting culture medium of D.bulbifera stem with a bud is MS + NAA 2 mg/L.

The performance evaluation of science and technology management personnels could stimulate their enthusiasm and advance the development of science.In this article,a 3-dimensional model was set up based on the evaluating criteria,the evaluating objects & the familiarity between the appraiser and the objects,which was simplified from several complicated factors necessary in the process of evaluation.Compared with the traditional methods,this 3D model sheds more light on choosing the critical factors,which helps improve the accuracy and rationality of scientific evaluation.

Background and purpose: Multidrug resistance of tumor cells is the main factor for the failure of chemotherapy. It is found that the apigenin has the anti-tumor effect, but its role in multidrug resistant cells was rarely reported. This study aimed to investigate the effect of apigenin on multidrug resistant breast cancer cell line MCF-7/ADR, and to explore the role of apigenin in reversing multidrug resistance. Methods: The MCF-7/ADR cells were cultured with different concentrations of apigenin, and the same cells were cultured with ADR in the control group. Thecell proliferation was detected by MTT, the cell cycle distribution was detected by PI, and the cell apoptosis was detect-ed by Annexin V/PI. The drug sensitivity in vitro was detected by the method of MTT, and the drug retention rate was detected by rhodamine 123 accumulation. The expression of P-gp protein was measured by Western blot, the RT-PCR method was used to detect the transcription of multidrug resistance gene MDR1. Results: The MCF-7/ADR cell prolif-eration was inhibited by the apigenin, the cell cycle progression was blocked by the apigenin, and the cell apoptosis was induced by the apigenin. There were significant differences between the apigenin group and the ADR group (P<0.05). The IC50 of ADR on MCF-7/ADR cell was (12.37±0.18) μg/mL with the apigenin effect, while the IC50 of ADR on MCF-7/ADR cell was (39.83±0.29) μg/mL without the apigenin effect (P<0.05). The reversal index was 3.22. The retention rate of rhodamine 123 in MCF-7/ADR cells in the apigenin group was higher than that in the ADR group. The MDR1 gene transcription level in MCF-7/ADR cells was higher than that in the MCF-7 cells, and the P-gp expression in MCF-7/ADR cells was higher than that in the MCF-7 cells. However, the level of MDR1 gene transcription and P-gp expression were down-regulated by the apigenin in the MCF-7/ADR cells. Conclusion: The apigenin had anti-MCF-7/ADR effect, and played the role of reversing multidrug resistance in the MCF-7/ADR cells. The mechanism may be related to down-regulation of the MDR1 gene transcription and the P-gp mediated drug e?ux function.

Objective To discuss the clinical value of intravascular ultrasound (IVUS) in treatinginternal carotid arteries occlusion.Methods The patient was diagnosed with internal carotid artery occlusionthat was confirmed by CTA.Cerebral perfusion imaging showed that low perfusion area was consistent withischemic symptoms.Guided by IVUS,percutaneous transluminal angioplasty (PTA) was performed.By usingmicro-catheter coaxial technology,the micro guide wire was inserted in the carotid artery until it passedthrough the obstructed segment;After IVUS examination proved that the micro guide wire was in the truelumen of carotid artery,angiography through micro-catheter was carried out to confirm that the distal arterywas unobstructed;after adjusting the device position the embolism protector was placed.The plaque andlumen condition were assessed with IVUS,which was reevaluated after pre-expansion of balloon.After normaldirection blood flow was regained,the plaque stability was assessed with IVUS virtual organization sequence.Simple balloon dilatation therapy was adopted as the fibrous cap of plaque was in stable condition and thelumen stenosis rate was ＜40％.Results After balloon dilatation,the obstructed artery was reopened and theblood flow regained normal direction.IVUS examination showed that during the whole operation process thefibrous cap of plaque at the narrowed segment remained in stable condition,the lumen stenosis rate was ＜40％.Cerebral perfusion imaging revealed that after the treatment the low perfusion state was markedlyimproved.Conclusion IVUS plays an important guiding role in performing PTA for internal carotid arteryocclusion.This technique can increase the success rate of vascular recanalization and reduce the incidence ofcomplications.

The scientific research management is one of the critical factors to build up the innovative system of scientific research.There are two modes of scientific research management:self-organization and hetero-organization.The serf-organization management,which follows the rules of science development,is the precondition to advance the scientific research,it is also necessary to implement the hetero-organization in right time and right place.The management should conduct more investigation into the nature of scientific research,put the service on the important role and finally speed up the reforming of science research management mechanism.

Objective: To investigate the relationship between the Smad4-independent pathway of TGF-?1 and drug-resistance of pancreatic cancer. Methods: The sensitivities of Smad4 homozygous-deleted pancreatic cancer cell line BxPC3 to different kinds of anti-cancer drugs (5-Fu, Gemzar, Oxaliplatin, Cisplatin, CPT-11 and Epirubicin) were observed by MTT assay before and after they were transfected with full-length cDNA of TGF-?1 or treated with TGF-?1 (5 and 10 ng/ml) solution. Western blot was used to detect p170 protein expression after stimulation with different concentrations of TGF-?1. Results: Cisplatin had the most powerful killing effect on BxPC3 cells, followed by Oxaliplatin, 5-Fu and CPT-11 with moderate effect and Gemzar and Epirubicin with the least effect. Cells transfected with full-length cDNA of TGF-?1 or treated with TGF-?1 solution became less sensitive to Cisplatin. Western blot revealed upregulation of p170 expression by TGF-?1. Conclusion: The Smad4-independent pathway of TGF-?1 can increase the drug resistance of pancreatic cancer cells through upregulating expression of p170.