Thyroid carcinomas are the most common endocrine malignancies,and the overwhelming majority of them is differentiated thyroid carcinoma (DTC).The major therapies of DTCs are surgical resection,thyroid stimulating hormone (TSH) inhibitory treatment and iodine radioisotope (131I) treatment.131I has been widely applied for the diagnosis and treatment of DTC,however,part of these patients may reduce or loss the uptake capacity of 131I owing to the alteration of sodium-iodide symporter gene,BRAF,paired box 8,microRNA and cytokeratin 19.These genes are particularly important in the treatment of DTC,which can be used as biomarkers in the treatment efficacy evaluation.

Objective To investigate the percent and clinical significance of regulatory T lymphocyte (Treg) in lung cancer patients.Methods Sixty-six cases of lung cancer patients were collected as lung cancer group,30 healthy person were collected as control group.The percent of Treg was detected and contrasted between the two groups.Results The percents of Treg in control group and lung cancer group were 0.0356 ± 0.0122 and 0.1263 ± 0.0318,respectively,there was significant difference between the two groups (P ＜ 0.01).The percent of Treg had no significantly related with gender,age,pathological type in lung cancer patients (P ＞ 0.05).The percent of Treg was significantly related with histological grade and clinical pathological stage (P＜ 0.05 or ＜ 0.01).Conclusion The abnormal elevation of the percent of Treg may be involved in the occurrence and development of lung cancer,and the percent of Treg can be used as the classification and prognosis of lung cancer patients.

Objective To compare the clinical effects of vacuum sealing drainage and traditional method on subcutaneous effusion after spinal operation .Methods From June 2009 to June 2011 ,52 cases were randomly divided into 2 groups:group A(treat with vacuum sealing drainage) and group B(treat with traditional method ) .Comparing the cure rate ,wound healing time ,infection rete and satisfaction rate between two groups .Results All cured cases were followed up for 3 months ,all wounds achieved primary healing postoperatively ,and without recurrence .The cure rate was 92 .31% (24/26)in group A ,there was statistically significant difference compared with group B[69 .23% (18/26)](χ2 =4 .457 ,P<0 .05) .The wound healing time was(12 .13 ± 1 .39) d in group A ,and(17 .72 ± 1 .78) d in group B ,which showed statistically significant difference between two groups (t=11 .455 ,P<0 .05) . The satisfaction rate was 84 .62% (22/26)in group A ,and 61 .54% (15/26)in group B ,which showed statistically significant differ-ence between two groups(χ2 =4 .457 ,P<0 .05) .Conclusion The clinical effects of vacuum sealing drainage on subcutaneous effu-sion after spinal operation was obviously superior to traditional method ,and vacuum sealing drainage has advantages such as less pain ,more satisfaction in patients and less workload in medical staff .

Objective To discuss the application of sperm mobility parameters in semen quality analysis. Methods The semen samples from 2343 outpatient males were examinated following theWorld Health Organization (WHO) guide, and sperm mobility parameters were also evaluated.Results There are significant difference of all sperm mobility parameters between normal and unnormal semen groups. As spermatozoa vialibity reduced, VCL、VSL、VAP、MAD、ALH、STR had decreased and BCF had increased significantly. Compared with the group which spermatozoa density and activity were both normal, LIN、STR had reduced and MAD、BCF had increased in the small quantity sperm group. All parameters were significant difference besides ALH in the lower sperm activity group. And for that in the group which spermatozoa density and activity were both unnormal, only MAD and ALH were no significant difference. Conlusion Sperm mobility parameters may serve as key parameters of semen quality analysis and play an important role in evaluating the ability of male fertility.

PURPOSE Anti-cancer drug treatment induces cell death by apoptosis, whether the gene p53 undergoes mutant changes is found to have a close relationship with anti-cancer drugs. The aim of this work is to investigate the role that p53 plays in the 5-FU-induced apoptosis of colorectal cancer cells in vitro. METHODS Thirty-nine col-orectal cancer samples from patients were treated with 5-FU (10?g/ml) and folinic acid (5?g/ml. 10?g/ml) in vitro, using the in situ Terminal Deoxynucleotidyl Transferase assay (TDT) to detect the chemosensitivity. p53 mutations from tumor DNA were detected, after amplication by PCR of exons 5 to 8. by non-radioactive single-strand conformation polymorphism(PCR-SSCP). RESULTS p53-gene mutations were observed in 43. 6% (17/39) of colorectal carcinomas. When TDT assay was used to detect the tumor apoptotic rate, cells with mutated p53 had less chemosensitivity than those without (16. 4% ?4. 89% vs 26. 6%? 6. 80% P

Objective To evaluate the effectiveness of interventional procedures in the treatment of acute pulmonary embolism(PE). Methods Pulmonary arterial thrombolytic therapy, suction and fragmentation of thromhi, anti-spasm, and balloon dilation were performed in eighteen cases with clinically highly suspected PE confirmed by emergent pulmonary artery angiography beforehand. Wedged pulmonary arterial pressure, pulmonary recanalization rate, the display ratio of distal pulmonary capillary net, blood gas analysis, blood oxygen saturation, and the improvement of clinical symptoms and signs were used for evaluation of the effectiveness. Seventeen of the alive cases with dislodgement of deep vein thrombi of the lower extremities were confirmed for the formation of PE and then thrombolytic treatment of the thrombotic deep vein was performed after the placement of inferior vena cava filter. Results Pulmanory artery angiographies showed embolism of the pulmonary arterial trunks or more than two of the branches. The post-treatment pulmonary patency reached 80%-90% in three cases, 90%-95% in eleven cases and 100% in five cases. The display ratio of distal pulmonary capillary net was over 90% in all the cases. Wedged pulmonary arterial pressure decreased to below 25 mmHg in thirteen cases, and to 25-30 mmHg in five cases. Blood oxygen saturation rate improved immediately to 90%-95% in ten cases and to 95%-100% in eight cases. Cough, hemoptysis, dyspnea, and chest pain were completely relieved in thirteen cases and significantly relieved in five cases. Digestive track bleeding was complicated in one case. Conclusions Pulmonary artery angiography is the golden standard for the diagnosis of pulmonary embolism. Interventional therapy is very effective for acute pulmonary embolism, which can significantly reduce the mortality rate.

Objective:To investigate the influence of p33~ING1b and wt-p53 on the biological activities of pancreatic cancer cells. Methods: Sense and anti-sense cDNAs of p33~ING1b were cloned into pcDNA3 eukaryotic expression vector separately, and the recombinant plasmids were solely or co-transfected with wt-p53 into pancreatic cell line SW1990. Flow cytometry was used to analyze the changes of cell cycle, Western blotting was used to evaluate the expression of p33~ING1b and p53 protein, and MG-P-MY staining was applied to observe cell apoptosis. Results: SW1990 cells solely transfected with sense p33~ING1b plasmid or co-transfected with wt-p53 and sense p33~ING1b plasmid expressed more p33~ING1b and had more apoptosis than the antisense p33~ING1b plasmid and mock transfected cells did (P

Objective: To investigate the relationship between the Smad4-independent pathway of TGF-?1 and drug-resistance of pancreatic cancer. Methods: The sensitivities of Smad4 homozygous-deleted pancreatic cancer cell line BxPC3 to different kinds of anti-cancer drugs (5-Fu, Gemzar, Oxaliplatin, Cisplatin, CPT-11 and Epirubicin) were observed by MTT assay before and after they were transfected with full-length cDNA of TGF-?1 or treated with TGF-?1 (5 and 10 ng/ml) solution. Western blot was used to detect p170 protein expression after stimulation with different concentrations of TGF-?1. Results: Cisplatin had the most powerful killing effect on BxPC3 cells, followed by Oxaliplatin, 5-Fu and CPT-11 with moderate effect and Gemzar and Epirubicin with the least effect. Cells transfected with full-length cDNA of TGF-?1 or treated with TGF-?1 solution became less sensitive to Cisplatin. Western blot revealed upregulation of p170 expression by TGF-?1. Conclusion: The Smad4-independent pathway of TGF-?1 can increase the drug resistance of pancreatic cancer cells through upregulating expression of p170.

In order to provide morphological data and theoretical basis for pig-to-human hepatic xenotransplantation, the difference in morphological parameters and vessel wall structural factors between human and porcine hepatic portal vein was studied. From human subjects and pigs of varying ages, hepatic portal veins were collected, paraffin-embedded and cut into sections. The histological structures were stained with HE, and elastin, collagen and smooth muscles were stained with Weigert, Aniline blue and orange G, respectively. Morphological parameters and relative contents of structural components were determined under microscopy and by computer image analysis system, respectively. The results showed that histological structures of human and porcine hepatic portal vein wall were similar. Caliber, wall thickness, lumen and wall area in pigs increased with age, all in linear correlation to months. Morphological parameters of 6- month-old pigs were similar to those of human. In pigs, collagen content increased gradually with months, elastin content remained relatively stable, smooth muscle content reached the peak at the 3rd month, and collagen/elastin (C/E) rose gradually. The contents of collagen and elastin in porcine hepatic portal vein wall were lower, while the content of smooth muscle was higher than in human, and C/E at the 5th and 6th month was similar to that in human. It is concluded that morphological parameters and contents of structural components of porcine hepatic portal vein vary with age. At the 6 month, its caliber, wall thickness, lumen and wall area are similar to those of human. There are differences in contents of structural components between human and pigs. However, in terms of C/E, mechanic properties of pigs at the 5th and 6th month mimic those of human, hence inosculation is viable in xenotrans-plantation between pigs and human.
Collagen/*analysis ; Elastin/*analysis ; Image Processing, Computer-Assisted ; Liver Transplantation ; Muscle, Smooth, Vascular/cytology ; Portal Vein/*anatomy & histology ; Portal Vein/chemistry ; Swine ; Transplantation, Heterologous

BACKGROUND: The source of conspecific heart transplantation organ is very limited and deficiency becomes more and more obvious.OBJECTIVE: To observe the mechanical properties of ascending aorta at one-dimensional loading between healthy persons and swine of different months, so as to provide necessary biomechanical experimental basis for anastomosing blood vessel in heterogeneic heart transplantation from swine to human.DESIGN: Open design SETTING: Staff Room of Anatomy, Yunyang Medical College MATERIALS: This experiment was carried out at the Laboratory of Biomechanics, Yunyang Medical College from April 2002 to July 2003.Ascending aortas of human were obtained from the 6 adult male corpses without cardiovascular diseases, aged 18 to 30 years, who died for accident and donated by Yunyang Medical College. Totally 42 conspecific swine of 1 month old, with certification number of QN0202, were provided by Animal Experimental Center of Yunyang Medical College. They were raised with common foodstuff. The 42 swine were butchered respectively at 1,2,3,4,5,6 and 7 months, 6 swine once. After anatomical isolation and in situ measurement of respective in vivo length, ascending aorta from aorta valve ring base plane to initiation part of innominate artery was taken out (Artherosclerosis was not presented in all the samples) and divided into five equal segments, and the second and fourth segments were used for mechanical test of one-dimensional loading.METHODS: Six adult male corpses and ascending aorta of 42 swine of 1 to 7 months were performed mechanical test of one-dimensional loading.All the blood segments were pre-treated ten times with the same strain rate at room temperature 32 ℃(loading range from 0 to 0.5 N). Hysteresis disappeared after blood vessel was given periodic permanent loading and unloading, and repeated force-deformed data were obtained. Blood vessel was given loading and unloading once with the same loading range and strain rate. The recorded force-deformed data were used for computer analysis. Force and displacement were scaled with standard weight and percentage scale at the end of each time. Constant α and elastic modulus Dt/dλ were fitted from experimental data.MAIN OUTCOME MEASURES: ① Comparison of the mechanical characteristic constant of ascending aorta at one-dimensional loading be tween human and swine of different months. ② Comparison of elastic mod ulus of ascending aorta at one -dimensional loading between human and swine of different months .RESULTS: ① Comparison of the mechanical characteristic constant of ascending aorta at one-dimensional loading between human and swine of different months: With the increase of month, although the material con stant of blood vessel of swine had a little increase, there was no significant difference (P ＞ 0.05). The mechanical material constant of ascending aorta at one-dimensional loading of human was basically familiar to that of swine of 1 to 7 months (P ＞ 0.05). ② Comparison of elastic modulus of ascend ing aorta at one -dimensional loading between human and swine of differ ent months: With the increase of months, elastic modulus of ascending aorta was significantly increased in swine of 7 months old in comparison with that in other months old (P ＜ 0.05); there was no significant difference of elastic modulus of corresponding blood vessel between human and swine of different months (P ＞ 0.05).CONCLUSION: There is no significant difference in mechanical characteristic constant and elastic modulus of ascending aorta between human and swine of 1 to 7 months. Mechanical characteristics of corresponding blood vessel were similar in some segments at least. From the mechanical angle, anastomosis of corresponding ascending aorta in the process of heterogeneic heart transplantation from swine to human has certain feasibility.