Object To study the effect of l stepholidine (l SPD) on ? adrenoceptors Methods The affinity of l SPD to ? adrenoceptors was investigated by radioligand binding assay by means of competitive [ 3H] labelled Dihydro Aprenolol ([ 3H] DHA) specific binding The antagonism of l SPD on ? adrenoceptors was tested on pig coronary artery rings in vitro. Results [ 3H] DHA binding assay showed that l SPD specifically displayed binding affinity to ? adrenoceptors The Ki value was (5 75?0 56) ?mol/L; the logarithmic cumulative concentration response curves (LCCRC) of l[WT5,5”BZ] SPD for NA on the pig coronary artery rings showed that the position of curves shifted to the right and the crest depressed The values of pA 2 an pD′ 2 were 7 21?0 78 and 5 32?0 13 respectively As a control, propranolol (Pro) could shift the curves parallelly to the right and the pA 2 value of Pro was 9 19?0 17 Conclusion The results suggested that l SPD is a ? adrenoceptor antagonist and has affinities to more than one kind of receptor

Objective An innovation of operative procedure with biological repair of ligaments for the treatment of acromioclavicular dislocation. Methods Based on the anatomy, physiology, and biomechanics, the operation was designed for restoring the stability of a completely dislocated acromioclavicular joint. Through bone holes drilled along the direction of coracoclavicular ligament fibers, the synthetic absorbable suture was used to rebuild the coracoclavicular ligament for vertical stability of the joint, and then a double loop suture was performed to rebuild acromioclavicular ligament and the capsule for horizontal stability. This biomechanical repair was simple, rapid procedure, and of less tissue trauma. The acromioclavicular joint was steadily fixed without any metallic imbedding, nor interference with joint surface. Results With 1－ 2.5 years follow up, 11 patients recovered with good healing, normal range of shoulder joint motion, and stable fixation. There was no need for secondary procedure to remove the sutures. The curative results of all patients were A degree based on the Karlsson s evaluation. There was no any complication. Conclusion The synthetic absorbable suture could be used with satisfaction for the repair of acromioclavicular dislocation. Compared with routine fixation with metallic material, the new kind of biofixation was not only effective, but also avoided the need of a second procedure for suture removal.

It is difficult to treat the internal carotid arterial (ICA) wall disease between C2 to C7 segment ( the definition of ICA by E. Bouthillier,1996) with present interventional radiological measurements , includling pseudoaneurysm, giant or wide neck aneurysm and complicated carotid cavernous fistula. Some literatures have reported a few cases treated with coronary covered stent (e.g. Jomed coronary stent, Symbiot coronary stent), with favorable results . However, these kinds of coronary covered stents are too rigid, with uneasinees to pass through in tortuous ICA, and often resulting in injury of vascular wall. The utility of covered stent in intracranial artery also caused controversies of exclusion of origins of arterial branches. We analyzed the functional anatomy of ICA and reviewed our experimental results and primarily clinical application on professional intracranial covered stent with special designed structure and delivery system for ICA, in order to explore the possibility of utility of covered stent in intracranial arteries.

Phenotypic conversion process of cells in blood vessels occurred after percutaneous transluminal angioplasty (PTA), which is similar to “recall” of angiogenesis. This article is an attempt in reviewing the major themes and more recent findings dealing with the phenotypic conversion and cellular source in three layers of arterial wall after PTA in the adult.

Objective:To construct subtractive cDNA library from human hepatocellular carcinoma cells transactivated by C-terminally truncated 40 amino acids using suppression subtractive hybridization(SSH) technique and to clone the associated genes.Methods: Huh-7 cells were separately transfected with pcDNA3(-) harboring the sequence of HBx protein C-terminally truncated 40 amino acids and pcDNA3(-) harboring the full length sequence of HBx protein vectors.The total RNAs were isolated from the transfected Huh-7 cells and were reversely transcripted into double strand cDNAs.After the cDNAs were digested with restriction enzyme RsaⅠ,they were divided into 2 groups and were ligated to the special adaptor 1 and adaptor 2R,respectively.The tester cDNAs were then hybridized with driver cDNAs twice and the products were amplified twice by nested PCR technique.The PCR products were connected with pUCm-T plasmid vectors to establish the subtractive library.Amplification of the library was carried out with E.coli strain JM109.The inserts of cDNAs were sequenced and analyzed in GenBank with Blast search.Results: The subtractive cDNA library was successfully constructed.The amplified library contained 154 positive clones,and colony PCR showed that these clones contained 200-800 bp inserts;some fragments coded proteins involved proto-oncogenes,cell signaling genes,cell growth factor genes,cell apoptosis genes,metabolism and protein synthesis genes.Conclusion: Subtractive cDNA library has been successfully constructed by SSH technique,which may help to clone novel genes transactivated by HBx C-terminally truncated 40 amino acids and to explore the molecular mechanism of hepatoma pathogenesis.

Objective] To evaluate the navel therapy of ancient formula for ehloasma of liver qi stagnation syndrome. [Method] The 40 cases take navel therapy of ancient formula, change every 24 hours for one month; make therapeutic effect observation before and after the treatment . [Result] 21 cases were cured, 10 had marked effect,7 had effective,2 had no effect, and the total effective rate was 95%.[Conclusion] Navel therapy of ncient formula has good cure effect on ehloasma.

In the last decades, the incidence of thyroid cancer has continuously and rapidly increased, and the risk factors have become the focus of the whole society′s attention.The exposure of radiation in childhood and genetic predisposition are determinate risk factors for thyroid cancer.Goiter, benign nodules/adenomas and obesity are highly likely risk factors for thyroid cancer.Some menstrual and reproductive factors and dietetic factors are possible risk factors for thyroid cancer.

Objective To investigate the value of intermediated cutting approach in the total thyroidectomy.Methods The clinical data of patients with total thyroidectomy from January 2009 to December 2011 were analyzed retrospectively.Patients in group A(n =134) were underwent thyroidectomy through intermediate approach,whereas patients in Group B (n =187) were underwent traditional total thyroidectomy.The analysis index included operation periods,operative bleeding amount,the incidence of hypocalcemia and rate of laryngeal nerve injure.Results The operation periods in Group A was shorter than that in group B ((65.4 ± 8.7) second vs.(90.1 ± 11.3) min,t =22.195,P ＜ 0.01).The amount of operative bleeding in group A was,less than that in group B ((20.5 ± 5.7) ml vs.(50.8 ± 11.4) ml,t =31.294,P ＜ 0.01).The incidence rates of temporary recurrent laryngeal nerve injure and the temporary hypocalcemia rate in group A and group B were (0.74％ vs.5.88％,10.44％ vs.24.59％ respectively,x2 =5.72,10.28 respectively,P ＜ 0.05).No permanent hypoparathyroidism or permanent recurrent laryngeal nerve injure case occurred in both groups.Conclusion Intermediate cutting approach was an effective and safe method in terms of total thyroidectomy.

Objective To evaluate the clinical applica ti ons of endoscopic thyroid surgery by way of breast areola. Methods We retrospectively analyzed clinical data of 15 cases of endoscopic thyroid surgery through the breast areola approach form August 2002 to June 2004. Proble ms on the selection of surgical methods, indications, contraindications and the safety of the procedures were discussed. Results The operation was completed smoothly in 14 cases, with an operation time of 60~120 min (mean, 100 min), whereas a conversion to open surgery was required in 1 case because o f hemorrhage. No complications were seen. The patients were fully freed of the d isease and discharged from the hospital 7 days after the operation. One patient was diagnosed as having thyroid cancer and was cured with a total thyroidectomy. Follow-up for 3~6 months (mean, 4.5 months) in 13 cases found no recurrence. Conclusions Endoscopic thyroid surgery through breast areola appro ach is safe and applicable, with good cosmetic effects.

Objective To explore the relationship between Helicobacter pylori (Hp) infection and psoriasis vulgaris, and investigate the effect of anti-Hp therapy on the patients with psoriasis vulgaris. Methods 13C-urea expiration test and detection of Hp antigen in feces samples were performed to identify Hp infection in 62 psoriasis patients and 35 healthy persons. Detection results of the two groups were statistically analyzed. The 62 psoriasis patients were divided into two groups based on the detection of Hp infection: Hp positive group (32 cases) and Hp negative group (30 cases). Patients in Hp positive group were given anti-Hp triple therapy (omeprazole, amoxicillin, clarithromycin) for two weeks and topical application of tretinoin for six weeks, while the patients in Hp negative group were treated only with topical use of tretinoin for six weeks. The total effective rate, score of psoriasis area, severity index (PASI) and visual analogue scale (VAS) of pruritus were recorded and then analyzed. Results The Hp infection rate in 62 psoriasis patients was 51.6%, and in healthy individuals was 22.9%, the difference between the two groups was statistically significant (P