1.An analysis on biomedical effects of bipolar electric pulses at different central frequency.
Yafang TAN ; Hongchun YANG ; Jun XU ; Yi ZHANG ; Minghe WU ; Heng ZOU
Journal of Biomedical Engineering 2012;29(3):438-442
Adopting the cell model of multilayer spherical symmetry and the circuit analysis, the present paper gives the calculated results of the voltages on each of several parts of malignant Tonsillar B-cells and Jurkat T lymphocytes when the first-order Gaussian pulses at different central frequency apposed on them. The relationship between the central frequency and the transmembrane voltages of plasma membrane is also given. The optimum frequency causing electroporation in nuclear envelope is given as well. The paper discusses the reasons of electroporation in membrane and DNA degradation in nuclear. The work provides a reference for usage of transient bipolar electric pulses in cancer treatment.
Apoptosis
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radiation effects
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B-Lymphocytes
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cytology
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radiation effects
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Cell Line, Tumor
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Cell Membrane
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physiology
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Electromagnetic Fields
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Electroporation
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methods
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Humans
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Jurkat Cells
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Nuclear Envelope
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pathology
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radiation effects
2.Guideline of surgical practice for non-small cell lung cancer based staging.
Yilong WU ; Qinghua ZHOU ; Meilin LIAO ; Guoliang JIANG ; Minghe ZHANG ; Xizeng ZHANG ; Jun WANG ; Xiuyi ZHI ; Gang CHEN ; Siyu WANG ; Xuening YANG ; Yan SUN ; null
Chinese Journal of Lung Cancer 2004;7(5):399-403
The clinical evidences of the guideline came from clinical trials based evidence-based medicine. Applied principle of the evidence was: systematic reviews, RCTs, the results from multiple factors ana-lysis, consensus, especially combined with Chinese experience and some lung cancer guidelines used in USA or Europe. All doctors who use the guideline in making therapeutic strategy must combine patients' conditions with the knowledge of biological behavior, dynamic change and response to treatment of lung cancer.
3.Electric pulse duration and windows effect of nuclear envelope.
Minghe WU ; Hongchun YANG ; Yi ZHANG ; Xlaoming ZHENG ; Gang ZENG ; Yafang TAN ; Yunqing SUN ; Heng ZOU
Journal of Biomedical Engineering 2011;28(3):602-606
Nuclear envelope voltages of T cells were analyzed with a lumped circuitry for cells in combination with frequency domain power density of Gaussian pulses and monocycle pulses. According to the differences in geometric and electric parameters between normal and malignant T cells, circuitry analysis was performed. Theoretical evaluations indicated that apoptosis of malignant T cells was of feasibility, which could be applied in cancer therapy. The evaluations were in accord with the published experimental findings.
Animals
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Apoptosis
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radiation effects
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Electric Stimulation
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Electromagnetic Fields
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Electrophysiology
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Fourier Analysis
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Humans
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Jurkat Cells
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Nuclear Envelope
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pathology
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radiation effects
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T-Lymphocytes
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cytology
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radiation effects
4.Application of gene chip technology for acupuncture research over the past 15 years.
Wenrui JIA ; Yue ZHANG ; Qiying GUO ; Qisheng SUN ; Qiulei GUO ; Zhi JI ; Fangyuan YANG ; He ZHAN ; He WANG ; Minghe SUI ; Zhongwei HOU ; Chaoyang WANG ; Qingguo LIU
Chinese Acupuncture & Moxibustion 2017;37(12):1358-1362
To explore the application of gene chip technology in the acupuncture research so as to provide evidences for the mechanism of acupuncture for regulating bodies. The literature on the application of gene chip technology in the acupuncture field from 2001 to 2016 was collected in PubMed, Springer, CNKI and WANFANG databases, which was analyzed and summarized. There were some achievements of the technology for acupuncture research, focusing on the five aspects, including the study of the relationship between meridian-point and viscera, the influencing factors of acupuncture effect, the effect and mechanism of acupuncture analgesia, the mechanism of acupuncture anti-aging, the effect and mechanism of acupuncture for diseases of each system. Gene chip technology plays an important role in researching acupuncture mechanism. It is an important technology for genomics study of acupuncture. However, there are also some disadvantages such as high cost, deficient data mining, non-uniform observation objects, deficient professionals, etc. All those need further resolution so as to promote the application of this technology in the acupuncture researching field.