2.Mechanism of chitosan inhibiting rabbit aortic smooth muscle cell prolif eration
Daxin WANG ; Zonggui WU ; Bin ZHOU ; Yuanying JIAN ; Ming YING ; Minghe CHENG
Academic Journal of Second Military Medical University 2001;22(2):159-160
Objective: To study the mechanism of chitosan i n inhibiting the proliferation of rabbit aortic smooth muscle cells(SMCs). Methods: By means of c-myc probe labelled with random primers and Northern blot hybridization, we examined the effect of chitosan on vascu lar SMC c- myc mRNA expression, which was stimulated by newborn bull serum (NB S,20%). Results: The oncogene c-myc mRNA expression incerased in cultured vascular SMC 24 h after NBS exposure. These effects were inhibite d by chitosan (20 μg/ml). Conclusion: Chitosan might inhibit the expression of vascular SMC c-myc mRNA stimulated by NBS, through which the proliferation of vascular SMC are inhibited.
3.An analysis on biomedical effects of bipolar electric pulses at different central frequency.
Yafang TAN ; Hongchun YANG ; Jun XU ; Yi ZHANG ; Minghe WU ; Heng ZOU
Journal of Biomedical Engineering 2012;29(3):438-442
Adopting the cell model of multilayer spherical symmetry and the circuit analysis, the present paper gives the calculated results of the voltages on each of several parts of malignant Tonsillar B-cells and Jurkat T lymphocytes when the first-order Gaussian pulses at different central frequency apposed on them. The relationship between the central frequency and the transmembrane voltages of plasma membrane is also given. The optimum frequency causing electroporation in nuclear envelope is given as well. The paper discusses the reasons of electroporation in membrane and DNA degradation in nuclear. The work provides a reference for usage of transient bipolar electric pulses in cancer treatment.
Apoptosis
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radiation effects
;
B-Lymphocytes
;
cytology
;
radiation effects
;
Cell Line, Tumor
;
Cell Membrane
;
physiology
;
Electromagnetic Fields
;
Electroporation
;
methods
;
Humans
;
Jurkat Cells
;
Nuclear Envelope
;
pathology
;
radiation effects
4.Electric pulse duration and windows effect of nuclear envelope.
Minghe WU ; Hongchun YANG ; Yi ZHANG ; Xlaoming ZHENG ; Gang ZENG ; Yafang TAN ; Yunqing SUN ; Heng ZOU
Journal of Biomedical Engineering 2011;28(3):602-606
Nuclear envelope voltages of T cells were analyzed with a lumped circuitry for cells in combination with frequency domain power density of Gaussian pulses and monocycle pulses. According to the differences in geometric and electric parameters between normal and malignant T cells, circuitry analysis was performed. Theoretical evaluations indicated that apoptosis of malignant T cells was of feasibility, which could be applied in cancer therapy. The evaluations were in accord with the published experimental findings.
Animals
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Apoptosis
;
radiation effects
;
Electric Stimulation
;
Electromagnetic Fields
;
Electrophysiology
;
Fourier Analysis
;
Humans
;
Jurkat Cells
;
Nuclear Envelope
;
pathology
;
radiation effects
;
T-Lymphocytes
;
cytology
;
radiation effects
5.Guideline of surgical practice for non-small cell lung cancer based staging.
Yilong WU ; Qinghua ZHOU ; Meilin LIAO ; Guoliang JIANG ; Minghe ZHANG ; Xizeng ZHANG ; Jun WANG ; Xiuyi ZHI ; Gang CHEN ; Siyu WANG ; Xuening YANG ; Yan SUN ; null
Chinese Journal of Lung Cancer 2004;7(5):399-403
The clinical evidences of the guideline came from clinical trials based evidence-based medicine. Applied principle of the evidence was: systematic reviews, RCTs, the results from multiple factors ana-lysis, consensus, especially combined with Chinese experience and some lung cancer guidelines used in USA or Europe. All doctors who use the guideline in making therapeutic strategy must combine patients' conditions with the knowledge of biological behavior, dynamic change and response to treatment of lung cancer.