Objective To investigate changes of coagulation function before and after percutaneous coronary intervention(PCI) in patients with coronary heart disease ,so as to provide evidence and guidance for clinical treatment .Methods A total of 78 patients with coronary heart disease who received PCI from September 2013 to August 2014 were regarded as subjects .The thrombin time (TT) ,thrombin original time (PT) ,activated partial thromboplastin time (APTT) and levels of fibrinogen (Fg) and antithrombin (AT) were detected before surgery and 4 ,14 h after surgery ,and conducted analysis and comparison .Results In the 78 cases of pa‐tients with coronary heart disease ,56 cases with single vessel disease ,19 cases with double vessel disease ,3 cases with three le‐sions ,and the total lesion count was 115 ;diseased vessels were implanted 101 ,35 and 8 stents respectively ,and a total of 144 stents were implanted .The TT and APTT before PCI was (14 .1 ± 0 .4) and (38 .4 ± 1 .5) s respectively ,and was (64 .1 ± 5 .8) and (73 .5 ± 15 .7) s respectively 4 h after surgery ,the differences were statistically significant (P<0 .05);while there were no statis‐tically significant differences of TT ,APTT ,PT ,and levels of Fg 24 h after surgery compared with those before PCI(P>0 .05) ,and these indicators were back to normal .Conclusion Routine monitoring TT and APTT 4 ,24 h after PCI could reflect coagulation function ,while routine monitoring may not be necessary for patients whose levels of AT are back to normal 4 h after surgery .

Objective To study the clinical characteristics,diagnosis,and treatment of paratyphoid fever A (fever A) complicated with severe kidney damage. Methods The data were retrospectively reviewed.Results The symptoms of poisoning and gastrointestinal symptoms as well as the slightly abnormal urinalysis (proteinuria, hematuria) appeared early, which got worse along with the progression of the disease. The symptoms of urinary system and azotemia appeared in the worst period. After treatment with antibiotic, 4 patients recovered early.Conclusion Paratyphoid fever A can cause severe kidney damage, with non-specific symptoms at the early stage. The seriousness of the abnormal of urine test can't be ignored as those provides the basis for the early diagnosis. The early use of antibiotic is the key to avoid and lessen the serverity of kidney damage.

Objective To establish a oligochip method for detection of HBV Lamivudine drug resistance and evaluate the clinical role of the chip system.Methods 388 HBV DNA positive sera from patients receiving lamivudine treatment and 559 chronic hepatitis B patients not receiving lamivudine treatment,and 359 sera from HBV DNA negative controls were assayed for HBV mutations utilizing oligonucleotide microarray.Meanwhile,these results were contrasted with Quantitative PCR and DNA sequencing method.Results The results of clinical evaluation shows that for the codons 528,552 and 555,the agreements between the microarray and sequencing data are 96.6%,98.5% and 100%,respectively.In the 559 samples,which were detected positive for HBV DNA by quantitative PCR,all but three weak positive samples were positive by the microarray,demonstrating an agreement of 99.7%.All the 359 HBsAg negative samples were shown to be negative for HBV DNA by the microarray method.Conclusions The HBV-Lamivudine oligochip is eligible to detecting wild type HBV and HBV lamivudine-mutants in patient's sera.It has great potential application of administration for lamivudine treatment in HBV patients.

Objective:To investigate the effects of a Baqia Huaihua Liangxue decoction on peripheral blood T helper 17 cells/Tregulatory cells(Th17/Treg)balance and the expression of related cytokines in elderly patients with psoriasis.Methods:A total of 124 elderly patients diagnosed with psoriasis and receiving treatment at our hospital from September 2018 to December 2019 were divided into the observation group and the control group according to the parity number table, with 62 cases in each group.All patients underwent conventional treatment and physical therapy, and, in addition, the observation group was treated with a Baqia Huaihua Liangxue decoction for 2 months.The blood indexes including levels of Th17, Treg, Th17/Treg, and Th17/Treg-related cytokines such as interleukin-17(IL-17), IL-22 and IL-23 in the two groups were measured before treatment and 2 months after treatment.The psoriasis area and severity index(PASI)was used to evaluate the severity of skin damage in the two groups.The traditional Chinese medicine(TCM)syndrome scores were evaluated and compared between the two groups.Adverse reactions during treatment were monitored and recorded.Results:After 2 months of treatment, the Th17 and Th17/Treg levels were lower[(1.0±0.1)% vs.(2.4±0.9)%, 0.2±0.1 vs.0.6±0.2, all P<0.05]and Treg levels were higher in the observation group than in the control group[(5.3±1.1)% vs.(4.3±1.0)%, P<0.05]. Levels of IL-17, IL-22 and IL-23 were lower in the observation group than in the control group after 2 months of treatment[(22.1±3.5)ng/L vs.(35.6±4.0)ng/L, (44.8±4.4)ng/L vs.(49.9±5.1)ng/L, (43.7±5.2)ng/L vs.(52.5±5.4)ng/L, all P<0.05]. The PASI score and TCM syndrome score were lower in the observation group than in the control group after 2 months of treatment[(2.9±1.0) vs.(6.1±2.3), (3.2±1.9) vs.(7.6±2.1), all P<0.05]. No adverse reaction occurred in the two groups during treatment. Conclusions:The Baqia Huaihua Liangxue decoction has a clear therapeutic effect in elderly patients with psoriasis.It can stabilize peripheral blood Th17/Treg balance, inhibit the expression of related cytokines, reduce the degree of skin damage and has no adverse reaction during treatment.

Objective:To explore the improvement effect of rhein lysinate (RHL)on the insulin resistance in the KK/HlJ diabetes mellitus (DM ) mice induced by streptozotocin (STZ ), and to elucidate its mechanism. Methods:The KK/HlJ diabetic mouse models were made by intraperitoneal injection of STZ (50 mg· kg-1 )and fed with DM diet.A total of 48 mice were divided into normal control group,model group,low dose of RHL group (25 mg·kg-1 )and high dose of RHL group (50 mg·kg-1 ) (n= 12).All the mice were treated for 16 weeks. The levels of fasting glucose (FBG),total cholesterol (TC)and triglyceride (TG)of the mice were measured by glucose oxidase method.HE staining was used to observe the morphology of pancreas tissue of the mice.The insulin level in pancreas islet tissue was detected by immunohistochemistry method.The levels of insulin and of C reactive protein (CRP)in serum and tumor necrosis factorα(TNF-α)and interleukin 6 (IL-6)in liver tissue of the mice were detected by enzyme linked immunosorbent assay (ELISA)method.The expression levels of glycogen synthesis related genes (PI3K,AKT and GSK-3β)phosphorylation were detected by Western blotting method. Results:Compared with model group,the levels of FBG,TG and TC of the mice in low and high doses of RHL groups were significantly decreased (P < 0.05),but there was no significant difference in the insulin level (P >0.05).Compared with model group,the levels of CRP in serum and the levels of TNF-αand IL-6 in liver tissue of the mice in low and high doses of RHL groups were significantly decreased (P <0.01).The HE staining results showed that compared with model group,the islet morphology of the mice in low and high doses of RHL groups was partially restored,and the occasional inflammatory infiltration was observed.The immunohistochemical staining results showed that compared with model group,the brown granular substance in the islets of the mice in low dose of RHL group was significantly reduced,which was disappeared in high dose of RHL group.Compared with model group,the expression levels of glycogen synthesis related genes (PI3K,AKT and GST-3β)phosphorylation of the mice in low and high doses of RHL groups were increased (P <0.01).Conclusion:RHL has improvement effect on the insulin resistance in the KK/HlJ DM mice induced by STZ,and the mechanism may be related to promoting the glycogen synthesis.