Objective To investigate changes of coagulation function before and after percutaneous coronary intervention(PCI) in patients with coronary heart disease ,so as to provide evidence and guidance for clinical treatment .Methods A total of 78 patients with coronary heart disease who received PCI from September 2013 to August 2014 were regarded as subjects .The thrombin time (TT) ,thrombin original time (PT) ,activated partial thromboplastin time (APTT) and levels of fibrinogen (Fg) and antithrombin (AT) were detected before surgery and 4 ,14 h after surgery ,and conducted analysis and comparison .Results In the 78 cases of pa‐tients with coronary heart disease ,56 cases with single vessel disease ,19 cases with double vessel disease ,3 cases with three le‐sions ,and the total lesion count was 115 ;diseased vessels were implanted 101 ,35 and 8 stents respectively ,and a total of 144 stents were implanted .The TT and APTT before PCI was (14 .1 ± 0 .4) and (38 .4 ± 1 .5) s respectively ,and was (64 .1 ± 5 .8) and (73 .5 ± 15 .7) s respectively 4 h after surgery ,the differences were statistically significant (P<0 .05);while there were no statis‐tically significant differences of TT ,APTT ,PT ,and levels of Fg 24 h after surgery compared with those before PCI(P>0 .05) ,and these indicators were back to normal .Conclusion Routine monitoring TT and APTT 4 ,24 h after PCI could reflect coagulation function ,while routine monitoring may not be necessary for patients whose levels of AT are back to normal 4 h after surgery .

According to the situation in hospital, this study investigate the the selection and training System for junior to senior faculty in terms of its guiding principles, funding, and initial achievement. The study concluded that the system is helpful for the researchers to foster research consciousness and accumulate experiences, and it also improves the overall quality of junior faculties.

Sodium prasterone sulfate (DHEAS) and dehydroepiandrosterone (DHEA) are endogenous steroid compounds, which are synthesized and secreted by adrenal gland.They are the precursors of steroid hormones,including sex hormone.Based on intracrine theory,the majority of postmenopausal women will have vaginal atrophy,which is caused by the absence of endogenous hormones,DHEAS or DHEA.Moderate supplementation of DHEAS can relieve the associated symptoms of vaginal atrophy.Many clinical researches have demonstrated that DHEAS can efficiently alleviate the related symptoms of vaginal atrophy.The concentration of androgen and estrogen in patients with long term use of DHEAS are still in the normal intracrine physiological range.Therefore,DHEAS may become the first drug for the treatment of vaginal atrophy in the future.

ObjectiveTo investigate the effect and mechanism of echinacoside （ECH） in improving liver injury in rats with acute pancreatitis by establishing a rat model of acute pancreatitis and liver injury. MethodsA total of 24 Sprague-Dawley rats were randomly divided into blank group （Con group）， control group （Con+ECH group）， acute pancreatitis group （AP group）， and acute pancreatitis+ECH intervention （AP+ECH group）. The rats were given intraperitoneal injection of 10 mg/kg ECH on day 7 before the establishment of the model of acute pancreatitis； at 24 hours after the last administration of cerulein， blood samples were collected via the abdominal aorta， and serum was separated for biochemical analysis including alanine aminotransferase （ALT）， aspartate aminotransferase （AST）， lactate dehydrogenase （LDH）， gamma-glutamyl transpeptidase （GGT）， alkaline phosphatase （ALP）， albumin （Alb）， total bilirubin （TBil）， cholinesterase， blood amylase （Amy）， and lipase （LPS）. HE staining was used to observe the histopathological changes of the pancreas and the liver； transmission electron microscopy （TEM） was used to observe the microstructural changes of pancreas and liver tissue； ELISA was used to measure the levels of interleukin-1β （IL-1β）， interleukin-16 （IL-6）， tumor necrosis factor-α （TNF-α）， and interleukin-10 （IL-10） in liver tissue homogenate； immunohistochemistry was used to measure the levels of TNF-α and p-p65 NF-κB in pancreas and liver tissue； Western blot was used to measure the expression levels of NF-κB pathway proteins in liver tissue. A one-way analysis of variance was used for comparison of continuous data between multiple groups， and the SNK test or the Dunnett’s T3 method was used for further comparison between two groups. ResultsCompared with the Con group， the AP group had significant increases in ALT， AST， GGT， LDH， ALP， TBil， Amy， and LPS （all P<0.01）， as well as significant increases in the levels of IL-1β， IL-6， IL-10， and TNF-α in liver tissue homogenate （all P<0.01）. ECH intervention reduced the levels of ALT， AST， GGT， LDH， ALP， TBil， AMY， and LPS and inhibited the secretion of IL-1β， IL-6， and TNF-α in rats with acute pancreatitis. HE staining showed that ECH intervention alleviated the vacuolar degeneration of acinar cells， inflammatory cell infiltration in pancreatic tissue， and the necrosis of hepatocytes compared with the AP group. TEM showed that compared with the AP group， there was a reduction in the degree of mitochondrial swelling in liver and pancreatic cells after ECH intervention. ECH intervention partially reversed the elevated expression levels of p-p65 NF-κB and TNF-α in liver and pancreatic tissue. In addition， the expression levels of MyD88， p-IκBα， p-IKKα， and p-p65 were upregulated in liver tissue of rats with acute pancreatitis， which could be partially reversed after ECH intervention. ConclusionEchinacoside can alleviate liver and pancreatic injury induced by acute pancreatitis by inhibiting the TLR4/MyD88/NF-κB pathway.