In this study, we cloned the NS3 gene from bovine viral diarrhea virus (BVDV) VEDEVAC strain. The result showed that the average P-distance of Pestivirus NS3 amino acid sequence was 0.07 and the VEDEVAC strain was classified to BVDV type 1. Using pET-30a(+) as vector and Escherichia coli Rosetta (DE3) as host, we obtained purified recombinant NS3 protein by Ni-NTA affinity chromatography. Western blotting analysis demonstrated that both BVDV positive serum and classical swine fever virus (CSFV) positive serum were able to recognize the recombinant NS3 protein. Indirect-ELISA assay indicated that the protein could be used as detection antigen.
Animals ; Cattle ; Cloning, Molecular ; Diarrhea Viruses, Bovine Viral ; genetics ; immunology ; Escherichia coli ; genetics ; metabolism ; Genetic Vectors ; genetics ; Peptide Hydrolases ; genetics ; immunology ; Phylogeny ; RNA Helicases ; genetics ; immunology ; Recombinant Proteins ; biosynthesis ; genetics ; immunology ; Sequence Analysis, Protein ; Viral Nonstructural Proteins ; genetics ; immunology

OBJECTIVETo explore the status of diabetes prevention awareness among Chinese residents in order to provide the scientific basis for making intervention strategy.

METHODSA sample of 18-60 years old residents in six provinces was investigated from March to April in 2013.

RESULTSTotally, 17.8% of respondents answered all questions correctly about the awareness of diabetes. As for "Diabetes is a common chronic disease" got highest recognition rate (86.2%), and "Diabetes can be cured" got the lowest rate, 48.3%. 50.6% of respondents answered all questions correctly about the diabetes symptoms; and "thirsty, drink more water" got the highest recognition rate (75.3%). 17.8% of respondents answered all questions correctly about the complications of diabetes; and "causes of diabetes ketoacidosis" got the lowest rate of 31.6%. 12.3% of respondents answered all questions correctly about the diabetes high-risk group; the correct recognition rate for "family history" was the highest (75.1%); and the "macrosomia" got the lowest rate of 20.4%. 74.2% of respondents answered all questions correctly about the diabetes preventive measure;the correct cognition rate of a reasonable diet was the highest (92.0%), and the weight control was the lowest (81.5%). In addition, there are significant differences of knowledge score between different areas and genders. City residents' knowledge score about diabetes related views (6.2 ± 1.8), typical symptoms (2.9 ± 1.4), complication (4.1 ± 2.3), high-risk groups (3.4 ± 1.7), prevention measures (3.7 ± 0.9) were higher than rural residents (respectively 5.2 ± 2.2, 2.7 ± 1.5, 3.2 ± 2.3, 2.9 ± 1.8, 3.3 ± 1.2) (P < 0.05). Female residents' knowledge score about diabetes related views (5.7 ± 2), typical symptoms (2.9 ± 1.4), complication (3.8 ± 2.3), high-risk groups (3.1 ± 1.8), prevention measures (3.5 ± 1) were higher than male residents (respectively 5.5 ± 2.1, 2.7 ± 1.5, 3.5 ± 2.4, 3.1 ± 1.8, 3.4 ± 1.2, 18.1 ± 6.8) (P < 0.05).

CONCLUSIONThe public is lack of diabetes prevention awareness, and city residents' knowledge score is higher than rural residents', females are higher than males.

Adult ; Asian Continental Ancestry Group ; China ; epidemiology ; Diabetes Mellitus ; Female ; Health Knowledge, Attitudes, Practice ; Humans ; Male ; Middle Aged ; Rural Population ; Sex Factors ; Urban Population ; Young Adult

OBJECTIVETo explore the status of diabetes-related behaviors and affecting factors among urban and suburban residents in China.

METHODSA sample of 18-60 years old residents from Zhejiang, Jiangsu, Shanxi, Henan, Yunnan and Qinghai provinces was investigated by using stratified cluster random sampling method from March to May in 2013, and 4 282 residents were recruited. The Questionnaire of Health Literacy of Diabetes Mellitus of the Public in China was used to conduct the survey. χ(2) test was used to compare the different risk behavior characteristics of diabetes. The results of the survey were standardized by the 6th national census data. Multiple regression analysis was used to understand the affecting factors to related behaviors.

RESULTSAfter data standardization, 14.3% sat more than 6 hours every day, 57.6% exercised less than 3 times every week, 21.3% had no regularly daily diet, 58.7% paid no attention to diet control, 15.7% and 7.8% residents preferred salty and oily diet, 51.4% had physical examination less than 1 time every year, 29.7% were smokers, and 9.2% often drank. Urban residents spent much more time on sitting (18.2%) than rural residents (8.4%) (P < 0.05), and the rates of lack of physical exercise(52.7%), irregular diet (17.2%), paying no attention to diet control (51.9%), smokers (23.6%) among urban residents were lower than rural residents (60.1%, 21.4%, 62.5% and 32.1% respectively) (P < 0.05). The rates of irregular diet (24.6%), paying no attention to diet control (63.5%), high-salt and high-fat diet (30.1%), smokers (57.7%), drinking (18.7%) among men were higher than those in females (15.5%, 52.1%, 23.2%, 2.2%, and 0.8% respectively) (P < 0.05); regions, genders, educational levels, occupations were related to relative behaviors of diabetes, residents in rural areas (t = 7.829, P < 0.01), males (t = -21.760, P < 0.01), lower education level (t = 0.771, P < 0.01), as well as institution personnel (t = -4.240, P < 0.01) had lower behavior scores, which meant they had more risk behaviors related to diabetes.

CONCLUSIONThere were diabetes-related risk behaviors among both urban and suburban residents in China, such as insufficient physical exercise, no diet control and regular medical examinations. These risk behaviors happened more in rural areas than in urban areas, men than women, older age group than lower age group.

Adult ; Age Factors ; Alcohol Drinking ; China ; epidemiology ; Demography ; Diabetes Mellitus ; Diet ; Diet, High-Fat ; Exercise ; Female ; Health Behavior ; Humans ; Male ; Middle Aged ; Occupations ; Risk Factors ; Rural Population ; Sex Factors ; Surveys and Questionnaires ; Urban Population ; Young Adult

OBJECTIVETo investigate the health literacy about diabetes prevention and control and its influencing factors among the public in China.

METHODSA multistage stratified random sampling method was applied to investigate urban and rural residents aged 18-60 years among six provinces in China during March and May, 2013. The Questionnaire of Health Literacy of Diabetes Mellitus of the Public in China was used to conduct the survey. The influencing factors of health literacy about diabetes prevention and control were analyzed by logistic regression. The results of the survey were standardized by the 6(th) national census data.

RESULTSA total of 4 416 persons were interviewed. A total of 4 282 valid questionnaires including 1 986 males (46.4%) and 2 296 females (53.6%) were obtained. The efficiency rate was 97.0%. The level of health literacy about diabetes prevention and control among the public in China was 32.7% (1 036/2 399). And this rate was 40.5%, 36.5% and 15.4% in east, middle and west of China, respectively. Take the west region as reference, the health literacy about diabetes prevention and control in east China was high (OR = 2.510, 95% CI: 1.931-3.263), central China was in the second place (OR = 2.083, 95% CI: 1.579-2.749). The health literacy about diabetes prevention and control among males was lower than that in females (OR = 0.488, 95% CI: 0.402-0.593). The higher education level the respondents had, the higher the health lireracy level about diabetes prevention and control. Taking the primary school and below education level as reference, the health lireracy level about diabetes prevention and control of respondents with a secondary education was higher (OR = 1.103, 95% CI: 0.812-1.499), and that of college or above degree was the highest (OR = 2.029, 95% CI: 0.460-0.870). The health literacy about diabetes prevention and control among civil servants and institution staff was high, the enterprise personnel was in the second place (OR = 0.632, 95% CI: 0.460-0.870), the literacy level among the rural residents was very low (OR = 0.339, 95% CI: 0.234-0.491). The health literacy about diabetes prevention and control among people who have a family history of diabetes was relatively higher (OR = 1.570, 95% CI: 1.222-2.018 ). The health literacy about diabetes prevention and control among people who considered that they have diabetes risk was higher than that among people who considered they didn't (OR = 1.821, 95% CI: 1.317-2.517).

CONCLUSIONThe east region, females, a high educational level, civil servants and public officers, having a family history of diabetes, self-assessed risk of getting diabetes were protective factors of health literacy about diabetes prevention and control.

Adult ; China ; epidemiology ; Demography ; Diabetes Mellitus ; Education ; Female ; Health Literacy ; Humans ; Logistic Models ; Male ; Middle Aged ; Occupations ; Sex Factors ; Surveys and Questionnaires ; Young Adult

OBJECTIVETo study the epidemiological status of diabetes' risk factors among urban and suburban residents and their perceptions of their own health status and risk of diabetes.

METHODSStratified multi-stage sampling method was used to conduct the survey among 18-60 years old residents from Zhejiang, Jiangsu, Shanxi, Henan, Yunnan, and Qinghai provinces in China from March to May in 2013. The Questionnaire of Health Literacy of Diabetes Mellitus of the Public in China was used to conduct the survey, and then the data were analyzed. The main contents of the questionnaire included diabetes mellitus knowledge, diabetes mellitus risk factors and diabetes mellitus risk self-assessment. The results of the survey were standardized by the 6(th) national census data.

RESULTSA total of 4 416 respondents were surveyed, and 4 282 valid questionnaires were successfully collected during the survey and the response rate was 97.0%. After weighted adjustment, the overweight and obesity rate of the residents in 6 provinces was 35.3%, abdominal obesity rate was 54.7%. 77.8% of the residents without diabetes had more than one risk factor, but only 8.5% thought they might got diabetes. The residents with high risk behavior possessing rates of diabetes were in eastern areas (79.7%), urban areas (80.7%), men (84.1%), aged from 45 to 60(100.0%), below primary school education level (83.4%) and enterprise personnel (79.7%). The residents with high risk consciousness of getting diabetes were in eastern areas (11.0%), urban areas (9.7%), men (9.7%), aged from 45 to 60 (12.3%), over college education level (12.7%) and the offices (14.7%). The results of logistic regression showed that residents in the middle areas (OR = 2.148, 95% CI: 1.633-2.920), urban residents (OR = 2.100, 95% CI: 1.611-2.738), male (OR = 2.488, 95% CI: 1.962-3.154), the older (OR = 1.102, 95% CI: 1.090-1.115) had a higher risk behavior rate of diabetes mellitus. Urban residents (OR = 2.784, 95% CI: 1.497-5.175), male (OR = 1.522, 95% CI: 1.152-2.011), the older (OR = 1.022, 95% CI: 1.009-1.035), college educational level (OR = 1.685, 95% CI: 1.033-2.749), with a higher score of health awareness(OR = 1.090, 95% CI: 1.061-1.120)had a higher risk consciousness to being diabetes mellitus.

CONCLUSIONThe urban and suburban residents in China had a high risk behavior possessing rates of diabetes, and a low risk consciousness of being diabetes.

Adult ; China ; epidemiology ; Diabetes Mellitus ; Health Knowledge, Attitudes, Practice ; Humans ; Logistic Models ; Male ; Middle Aged ; Obesity ; Risk Factors ; Suburban Population ; Surveys and Questionnaires ; Urban Population