1.Screening of a High Growth Influenza B Virus Strain in Vero Cells
Ze LIU ; Weidong LI ; Mingbo SUN ; Lei MA ; Ziquan GUO ; Shude JIANG ; Guoyang LIAO ; Jingsi YANG ; Changgui LI
Virologica Sinica 2010;25(1):65-70
Due to the insufficient supply of embryonated chicken eggs,the preparation of large quantities of inactivated influenza vaccines will require an alternative virus culture system after the emergence or reemergence of a pandemic influenza virus.The Vero cell is one of the ideal options since it was used for producing many kinds of human vaccines.However,most of the influenza viruses can not grow well in Vero cells.To develop a new influenza vaccine with Vero cells as a substrate,the virus needs to adapt to this cell substrate to maintain high growth characteristics.By serial passages in Vero cells,the B/Yunnan/2/2005va(B)strain was successfully adapted to Vero cells,with the hemagglutination titer(HAT)of the virus reaching 1:512.The high growth characteristic of this strain is stable up to 21 passages.The strain was identified by hemagglutination inhibition (HAI)test and sequencing respectively;the HA;gene sequence of the virus was cloned and analyzed.The screening and establishment of high growth B virus provides an important tool for influenza vaccine production in Vero cells.
2. The replantation of penetrating severed finger with disconnected micro tissue blocks in 16 cases
Jun SONG ; Mingbo GUO ; Jingliang ZHANG ; Zhiyong REN ; Zengyun LIU ; Zhongming HUANG ; Qiaohong GUO
Chinese Journal of Microsurgery 2019;42(6):533-535
Objective:
To explore the method of replanting severed piercing finger micro tissue blocks.
Methods:
From February, 2001 to October, 2016, 16 cases of severed piercing finger micro tissue blocks were replanted with debridement. The trunk or branch of proper palmar digital artery was anastomosed. Vascular collocations were used such as venous arterialization when there was no anastomotic artery available. Regular postoperative followed-up was performed to observe the recovery of tissue survival, appearance, sensation of flap, and joint function.
Results:
One case had partial epidermal necrosis, and healed after change of dressing. The finger-disconnected micro tissue blocks of other 15 cases survived well. After 6 months to 2 years of follow-up, the replanted micro tissue blocks were ruddy in color and recovered from S3 to S4. The appearance of the 16 fingers was good, and the function of joints was restored.
Conclusion
The finger-disconnected micro tissue blocks should be actively replanted. With skilled microsurgical techniques, the anastomosis of the arterial or venous artery will ensure the success of replantation and survived, as well as to restore good function and appearance.
3.Four new phenolic glycosides from Baoyuan decoction.
Xiaoli MA ; Xiaoyu GUO ; Mingbo ZHAO ; Pengfei TU ; Yong JIANG
Acta Pharmaceutica Sinica B 2017;7(2):173-178
Four new phenolic glycosides, including two flavonoid glycosides (and) and two lignan glycosides (and), were isolated from the traditional Chinese medicine formula, Baoyuan decoction. Their structures were established by detailed analysis of the NMR and HR-ESI-MS spectroscopic data and their absolute configurations were determined by the experimental electronic circular dichroism data as well as chemical methods. Furthermore, the sources of the four new compounds were determined by the UPLC-Qtrap-MS method, which proved thatandare originated from, andandare from.
4.Thoracoscopic combined subsegmentectomy for 76 patients: A retrospective study in a single center
Bicheng ZHAN ; Jian LIU ; Jian CHEN ; Yongzhi LIU ; Genshui LI ; Kunliang GUO ; Xiao WANG ; Yanzheng XIONG ; Mingbo GU
Chinese Journal of Clinical Thoracic and Cardiovascular Surgery 2023;30(01):47-51
Objective To summarize the clinical experience of thoracoscopic combined subsegmentectomy (CSS). Methods The clinical data of 76 patients who underwent thoracoscopic CSS in Anqing Municipal Hospital from May 2018 to July 2022 were retrospectively analyzed, including 22 males and 54 females, aged 27.0-76.0 (54.3±10.5) years. All patients underwent preoperative three-dimensional computed tomography bronchography and angiography using dual source CT. The modified inflation-deflation technique or indocyanine green was used to identify the intersubsegmental border. Results A total of 86 pulmonary nodules were resected in 76 patients. One patient of left upper lobe S1+2c+S4a, 1 patient of right upper lobe S2b+S3a and 1 patient of right upper lobe S1b+S3b were further performed lobectomy due to insufficient margin. One patient of left upper lobe S1+2+S3a was further performed left upper division segmentectomy due to residual atelectasis. One patient of left upper lobe S1+2c+S3a was further performed left upper division segmentectomy due to B3b+c injury, and the rest completed planned surgeries successfully. The operative time was 90.0-350.0 (174.9±53.2) min. The operative hemorrhage volume was 50.0 (20.0, 50.0) mL. The postoperative hospital stay time was 6.0 (5.0, 7.0) d. Postoperative complications included pulmonary infection in 9 patients, hemoptysis in 3 patients, persistent pulmonary leakage>3 d in 4 patients, pneumothorax in 1 patient, pleural effusion in 1 patient, and myocardial infarction in 1 patient. All of the patients were cured and discharged without perioperative death. Conclusion Thoracoscopic CSS is relatively complex. Preoperative planning under three-dimensional reconstruction and intraoperative fine operation are helpful for safe completion.
5.Efficacy and safety of camrelizumab monoclonal antibody combined with molecular-targeted therapy in elderly patients with advanced hepatocellular carcinoma
Long CHENG ; Yue ZHANG ; Yushen LIU ; Zhaoqing DU ; Zhaoyang GUO ; Yangwei FAN ; Ting LI ; Xu GAO ; Enrui XIE ; Zixuan XING ; Wenhua WU ; Yinying WU ; Mingbo YANG ; Jie LI ; Yu ZHANG ; Wen KANG ; Wenjun WANG ; Fanpu JI ; Jiang GUO ; Ning GAO
Journal of Clinical Hepatology 2024;40(10):2034-2041
ObjectiveTo investigate the efficacy and safety of camrelizumab monoclonal antibody combined with molecular-targeted therapy in elderly patients with unresectable or advanced hepatocellular carcinoma (HCC). MethodsA retrospective analysis was performed for the patients with unresectable/advanced HCC who attended six hospitals from January 1, 2019 to March 31, 2021, and all patients received camrelizumab monoclonal antibody treatment, among whom 84.8% also received targeted therapy. According to the age of the patients, they were divided into elderly group (≥65 years) and non-elderly group (<65 years). The two groups were assessed in terms of overall survival (OS), progression-free survival (PFS), objective response rate (ORR), disease control rate (DCR), and immune-related adverse events (irAE). The chi-square test or the Fisher’s exact test was used for comparison of categorical data between groups; the independent samples t-test was used for comparison of normally distributed continuous data, and the Mann-Whitney U test was used for comparison of non-normally distributed continuous data between two groups. The Kaplan-Meier method was used for survival analysis, and the log-rank test was used for comparison of survival curves. Univariate and multivariate Cox proportional hazards regression analyses were used to determine the independent influencing factors for PFS and DCR at 6 months. ResultsA total of 99 HCC patients were enrolled, with 27 in the elderly group and 72 in the non-elderly group. The elderly group had an OS rate of 67.8%, an ORR of 44.4%, and a DCR of 74.1% at 12 months and a median PFS of 6.4 (95% confidence interval [CI]: 3.0 — 12.4) months, with no significant differences compared with the non-elderly group (all P>0.05). The median OS was unavailable for the elderly group, while the non-elderly group had an OS of 18.9 (95%CI: 13.0 — 24.8) months; there was no significant difference between the two groups (P=0.485). The univariate and multivariate Cox regression analyses showed that major vascular invasion (MVI) was an independent risk factor for PFS (hazard ratio [HR]=2.603, 95%CI: 1.136 — 5.964, P=0.024) and DCR (HR=3.963, 95%CI: 1.671 — 9.397, P=0.002) at 6 months, while age, sex, etiology of HBV infection, presence of extrahepatic metastasis, Child-Pugh class B, and alpha-fetoprotein>400 ng/mL were not associated with PFS or DCR at 6 months. For the elderly group, the incidence rates of any irAE and grade 3/4 irAE were 51.9% and 25.9%, respectively, with no significant differences compared with the non-elderly group (P>0.05), and skin disease was the most common irAE in both groups (39.4%). ConclusionCamrelizumab monoclonal antibody combined with molecular-targeted therapy has similar efficacy and safety in patients with unresectable/advanced HCC aged ≥65 years and those aged <65 years. MVI is associated with suboptimal response to immunotherapy and poor prognosis.