Objective To study the effects of lanthanum on cell multiplication and intracellular Ca2+([Ca2+]i) concentration in primary cultured astrocytes of rats.Methods The primary cultured astrocytes of rats were exposed to 0.25,0.5,1.0 and 2.0 mmol/L LaCl3 for 24 h and 48 h.The cell multiplication was measured by MTT;[Ca2+]i concentration was determined by the fluorescent Ca2+ indicator Fura-2/AM.Results After astrocytes were incubated with 0.25,0.5,1.0 and 2.0 mmol/L LaCl3 for 24 h and 48 h,the survival rate of astrocytes was 86.24% and 82.99%,82.26% and 79.14%,52.32% and 49.97%,38.04% and 35.15% respectively,which was significantly decreased compared with the control group(P

Adult ; Aged ; Cantharidin ; therapeutic use ; Carcinoma, Hepatocellular ; therapy ; Embolization, Therapeutic ; Female ; Humans ; Liver Neoplasms ; therapy ; Male ; Middle Aged ; Portal Vein ; Treatment Outcome

Adult ; Catheter Ablation ; methods ; Humans ; Male ; Sinus of Valsalva

Adult ; Aged ; Bone Transplantation ; Female ; Fibula ; transplantation ; Free Tissue Flaps ; Humans ; Male ; Mandible ; surgery ; Maxilla ; surgery ; Middle Aged ; Muscle, Skeletal ; transplantation ; Reconstructive Surgical Procedures ; methods

Adolescent ; Defibrillators, Implantable ; Female ; Humans ; Long QT Syndrome ; therapy

Animals ; Cell Line, Tumor ; Colonic Neoplasms ; genetics ; metabolism ; pathology ; Enzyme-Linked Immunosorbent Assay ; Humans ; Interleukin-18 ; biosynthesis ; genetics ; Mice ; Mice, Nude ; Neoplasm Transplantation ; RNA, Messenger ; biosynthesis ; genetics ; Reverse Transcriptase Polymerase Chain Reaction ; Survival Analysis ; Time Factors ; Transfection ; Transplantation, Heterologous

Objective To detect the expression of E-cadherin and ?-catenin in breast carcinoma and analyze the relationship between E-cadherin and ?-catenin and the clinicopathological features. Methods The expression of E-cadherin and ?-catenin in breast cancer, paracarcinoma breast tissue, simple mastoplasia and atypical mastoplasia were detected by immunohistochemical method and the results were compared. Results The abnormal expression rates of E-cadherin and ?-catenin in breast cancer tissue were 51.9% and 61.1 %,respectively. Abnormal expression of E-cadherin was significantly correlated with histological grade. Abnormal expression of ?-catenin was significantly correlated with TNM staging, axillary lymph nodes metastasis and postoperative distant metastasis. COX multiple factor analysis showed that neither E-cadherin nor ?-catenin expression was an independent indicator for the prognosis of breast cancer. Conclusions Abnormal expressions of E-cadherin and ?-catenin are correlated with occurrence and development of breast carcinoma. Abnormal expressions of E-cadherin and ?-catenin are good indicators to judge invasion and metastasis of breast carcinoma.

Cardiac Pacing, Artificial ; Electrodes, Implanted ; Female ; Humans ; Middle Aged ; Postoperative Complications ; etiology ; Subclavian Artery ; injuries ; Treatment Outcome

Objective To discuss the value of applying psychological therapy for patients with cerebral apoplexy in recovery.Methods In recent years,65 patients with cerebral apoplexy admitted in our hospital were randomly selected and divided into observation group and control group.Patients in the control group only received the conventional treatment and those in the observation group received psychological treatment on the basis of the traditional therapy.Psychological characteristics,activity of daily living and the improvement of nerve function were compared between two groups before and after treatment.Results After treatment,mental improvement,FMA,MBI in the observation group were significantly better than those of control group (P＜0.05).Conclusion To improve the prognosis of patients with cerebral apoplexy effect,physical and mental development should be equally emphasized.Psychological intervention has become a key for stroke rehabilitation treatment,which significantly improves the treatment compliance,rehabilitation and the quality of life in patients.

Objective To observe the effects of hypnosis therapy on rehabilitation of patients with spinal cord injury.Methods The data were randomly selected from 66 cases of patients with spinal cord injury treated in our hospital from November 2011-November 2012.According to the treatment,the patients were divided into research group and control group.Control patients were given routine therapy,and patients in the research group were given hypnosis therapy,we observed and compared the recovery of two groups of patients after treatment.Results After treatment,the spinal cord function score (SCIM) of patients in the research group was better than control group,the difference was statistically significant (P ＜ 0.05) After treatment,the depression and anxiety scale (HAMD,HAMA) of patients in the research group was better than control group,the difference had statistical significance (P ＜ 0.05) The quality of life after treatment of research group patients (WHOQOL BREF) was better than control group,the difference had statistical significance (P ＜ 0.05).Conclusion Hypnosis therapy helps to improve depression and anxiety in patients with spinal cord injury,improve patients with spinal cord function independently,should be widely used in clinical treatment and promotion.