Objective To evaluate the clinical effects and adverse reaction of somatostatin combined with raw vegetable oil in treatment of intestinal obstruction From Tongchuan City People's Hospital were conducted through clinical medication.Methods From August 2012 to August 2015,A total of 364 cases of intestinal obstruction were randomly divided into observation group (182 cases) and control group (182 cases).Two groups were treated with the non-surgical treatment based on basic therapy,including gastrointestinal decompression,correctional of water,electrolyte disturbance and acid-base imbalance to prevent infection and toxication by sedative and spasmolytic.The non-surgical treatment was invalid or worse,the surgical treatment wasselected.Additionally,on the basis of non-surgical treatment,somatostatin combined with raw vegetable oil was additional treatment for the observation group.The comparison of thecurative effect,surgical treatment rate,gastrointestinal decompression,hospital stays and adverse reaction of two groups was conducted.Results The curative effect of observation group (80.22％)was superior to control group(68.68％).Compared to control group,the cases of adverse reaction in observation group was insignificantly increased (x2 =8.58,P ＜ 0.05).Conversely,the observation group showed lower surgical treatment rate (29.67％ vs 40.11％,x2 =4.37,P ＜ 0.05),lower gastrointestinal decompression [(224 ± 171) ml/d vs (543 ± 186) ml/d,t =-21.28,P ＜0.05)],and less hospital stays [(6.14 ± 2.04) d vs (10.26 ± 3.12) d,t =-4.78,P ＜ 0.05)].Conclusions The basic therapy and somatostatin combined with raw vegetable oil showed high efficiency,and cases of adverse reaction was insignificantly increased.It was worthy of promotion and application.

Animals ; Drugs, Chinese Herbal ; pharmacology ; Glucosides ; metabolism ; Humans ; Infertility ; chemically induced ; Models, Animal ; Tripterygium ; metabolism

Humans ; Radiation Injuries ; drug therapy ; prevention & control ; Radiation, Nonionizing ; Radiation-Protective Agents ; therapeutic use ; Radioactive Hazard Release ; prevention & control

Actinin ; genetics ; Adolescent ; Adult ; Asian Continental Ancestry Group ; genetics ; Athletes ; Athletic Performance ; China ; Ethnic Groups ; genetics ; Female ; Humans ; Male ; Polymorphism, Genetic ; Young Adult

Objective To explore the safety and efficacy of endoscopic retrograde cholangiopancreatography(ERCP)by using carbon dioxide for malignant biliary obstruction.Methods A total of 60 patients with malignant biliary obstruction, hospitalized from September 2008 to February 2010, were randomly divided into 3 groups to receive ERCP by using carbon dioxide, contrast agent or magnetic resonance cholangiopancreatography(MRCP)without any agent.The safety and efficacy of 3 contrast methods were compared.Results Successful stent or drainage tube insertion was achieved in all patients.Carbon dioxide was superior to contrast agent in terms of lowering postoperative infection rate(P ＜ 0.05), and it was also superior to MRCP for more effective drainage(P ＜ 0.05).There was no significant difference in occurrence of postoperative pancreatitis and hyperamylasemia between 3 groups.Conclusion Using carbon dioxide during ERCP, safe and effective, is superior to use of contrast agent in less leukocyte increase, and to MRCP in more adequate drainage in patients with malignant biliary obstruction.Furthermore, there is no more risk of pancreatitis and hyperamylasemia.

AIM: To establish the method of determining peoniflorin,ferulic acid and icariin in Bushen Huoxue Granule(Herba Epimedii,Radix Angelicae Sinensis,Radix Paeoniae Alba,etc.).METHODS: HPLC-DAD was used to determine peoniflorin,ferulic acid and icariin.The samples were separated on Alltima C18(5 ?m,250 mm ? 4.6 mm) with mobile phase consisted of methanol-0.1% phosphoric acid(gradient elution).RESULTS: The average recoveries of peoniflorin,ferulic acid and icariin were 96.7%,97.5%,99.6% and RSD were 1.37%,3.13%,0.96%.respectively.The peoniflorin,ferulic acid and icariin samples showed a good relationship in the ranges of 0.318-3.970 ?g,0.096-1.200 ?g,0.414-5.175 ?g in the chosen HPLC condition.CONCLUSION: The method is simple,accurate and reproducible and can be used for the quality control of granule.

Objective To investigate the protective mechanism of hyaluronic acid (HA) antagonistic to nitmprusside sodium (SNP) on the tissue engineering cartilage. Methods Alginate culture for two weeks was used to recover phenotype of dedifferentiated chondrocytes. Differentiation state of chondrecytes was analyzed by immunostaining. The growth of alginate-recovered chondrocytes on the chitosan-based scaffold was observed by scanning electron microscope. After cultured for 3 weeks, this tissue engineering cartilage was treated with SNP in the absence or presence of HA combined with specific β1 integrin blocking antibody collagen type Ⅱ and aggreean were detected by RT-PCR and Western blot. Results Collagen type Ⅱ expression in dedifferentiated chondrocytes was significantly enhanced by alginate bead culture. The chitosan-based scaffold supported cell adhesion, proliferation and migration. A dose-dependent inhibitory effect on the expression of collagen type Ⅱ and aggrecan was observed when tissue engineering cartilage was treated with SNP alone. HA significantly promoted collagen type Ⅱ, and aggrecan expression antagonistic to low concentrations of SNP (p<0.05). However, the specific β1,integrin blocking antibody abrogated the effects of HA. Conclusion Alginate culture recovers the phenotype of dedifferentiated chondrocytes. HA abrogats the inhibitory effect of SNP via β1 integrin signal pathway to protect tissue engineering cartilage.

Objective To evaluate the clinical utility of SpyGlass single-operator ERCP-guided cholangiopancreatoscopy system for the diagnosis and treatment of bile duct disorders.Methods A total of 9 patients with biliary diseases were treated with SpyGlass single-operator ERCP-guided cholangiopancreatoscopy system.Four patients with large bile duct stones accepted laser lithotripsy,and stones were removed by ERCP.Five patients with indeterminate bile duct stricture accepted cholangioscopy-guided sampling.All patients underwent ENBD,routine blood test,blood amylase test,oral food and water restriction,acid suppression and prophylactic antibiotics.Results SpyGlass was successful in all patients with a manipulating time of 21.2min and total procedure time of 46.2min.Complete stone clearance was achieved in all 4 patients with large biliary stones.Sample quality was adequate in all patients with bile duct stricture with 2 patients diagnosed as having malignant biliary tumor and 3 as inflammatory bile duct stenosis.Post-ERCP complications including mild pancreatitis in 2 patients,and the patients recovered gradually after corresponding treatment.Conclusion ERCP-guided cholangiopancreatoscopy with the SpyGlass system is technically feasible and can be successfully and safely performed in patients with biliary disorders.

Objective To determine the sensitivity of autofluorescence bronchoscopy (AFB) in the assessment of tumor size and therapeutic strategy.Methods Patients with imaging suspected of malignancy were examined with both white light bronchoscopy (WLB) and AFB.The area of tumor infiltration,imaging information and pathological results were analyzed.Results A total of 212 patients were enrolled,including 180 male and 32 female.In 24 patients (13.2％),greater tumor volume was revealed by AFB than by WLB alone.In these patients,the median diameter of tumor was ＞ 1 cm wider on AFB examination than on WLB.Therapeutic strategy was changed in 18 patients (9.9％) after receiving AFB,including 15 patients with expanded scope of removal and 3 patients with avoidance of surgery.In the univariate analysis,the pathological type of squamous cell carcinoma and tumor invasion in two or more segments of bronchus were independent predictive factors.Diagnostic sensitivity of AFB group was 85.7％,specificity 73.3％,positive predictive value 95.1％,false predictive value 45.8％.Diagnostic sensitivity of WLB group was 72.5％,specificity 60.0％,positive predictive value 91.7％,false predictive value 26.5％.Conclusion Our study suggests that compared with WLB alone,autofluorescence bronchoscopy plus WLB significantly improves the diagnostic value and treatment outcome of central lung cancer.

Objective To evaluate the safety and efficacy of the endoscopic resection of large nonampullary duodenal adenomas.Methods Twenty cases with large nonampullary duodenal adenomas(≥ 1.0 cm) were referred for EMR.After submucosal injection of the mixture of glycerin fructose solution and methylene blue,either en bloc or piecemeal snare polypectomy was performed.All resected specimens were retrieved for pathologic study.Follow-up gastroscopy was performed in patients after EMR.Results Among 20 lesions,six were found located in the 1st part(30％),13 were in the 2nd part(65％),and one in the 3rd part(5％)of the duodenum.The mean diameter of the lesions was 1.9 ± 1.1 cm.Endoscopic ultrasonography (EUS) was performed in 12 patients,and all lesions were originated from the mucosa.EMR was performed successfully in 20 patients.The en bloc resection rate was 75％ (15/20),and the mean time for EMR was 33.8 ± 16.7 min.The perioperative complication rate was 20％ (4/20).Bleeding occurred within 24 hours after EMR in 4 cases.There were no perforations.The complete resection rate was 100％.Of these 20 adenomas,14 adenomas were tubulous and 6 was tubulovillous.During the follow-up period (2-39 months),1 patient showed recurrence within 6 months after EMR.Conclusion Endoscopic resection of large nonampullary duodenal adenomas is safe and effective treatment.