Anal Canal ; pathology ; surgery ; Humans ; Laparoscopy ; methods ; Rectal Neoplasms ; pathology ; surgery

Dimethyltrilobine iodide (DMT) 1.5 M-M increased the contractile force of isolated guinea pig left atria. This positive inotropic action could not be an-tagonized by either propranolol, cimetidine or diphen-hydramine. DMT 1.5 nM also increased the contractile force of isolated guinea pig left atria exposed to low concentration of calcium or to verapamil.

Objective:To develop an HPLC method for the content determination of paeoniflorin, tetrahydropalmatine and osthole in Gujinwan capsules. Methods:The determination was performed on an Eclipse XDB-C18 column (250 mm × 4. 6 mm,5 μm) with the mobile phase consisting of 0. 1％ phosphoric acid (adjusting pH to 6. 6 with triethylamine)-acetonitrile with gradient elution. The flow rate was 1. 0 ml·min-1 . The column temperature was 30 ℃. The detection wavelengths were set at 230 nm and 280 nm. Re-sults:The calibration curves were found to be linear within the range of 9. 56-33. 65 μg· ml-1 for paeoniflorin(r=0. 9998), 3. 65-12. 86 μg· ml-1for tetrahydropalmatine(r=0. 9999) and 5. 81-20. 45 μg· ml-1for osthole(r=1. 0000). The average recovery of paeoniflorin, tetrahydropalmatine and osthole was 98. 8％(RSD=1. 1％), 98. 4％(RSD=0. 8％) and 99. 1％(RSD=1. 4％)(n=6),respectively. Conclusion:The method is simple, accurate and reproducible, which can be used for the quality control of Gujinwan capsules.

Thin basement membrane nephropathy(TBMN) is one of the most common disorders of the kidney,affecting at least 1% of the population.It seems to be a disease of the adult glomerular basement membrane(GBM) type Ⅳ collagen trimer ?3∶?4∶?5.Genetic evidence indicates that autosomal TBMN is caused by heterozygous mutations in either COL4A3 or COL4A4, whereas homozygous or combined heterozygous mutations in the same genes lead to autosomal recessive Alport syndrome.The author summarized the epidemiology,clinical features,renal biopsy,genetics,pathogenesis,diagnosis and therapy of TBMN.

The pffect of 5 drugs on myocardial nutritional blood flow of micehave been determined with ~(86)Rb. It was shown that the root of xanthiumsibiricam, caesulpinia sepiaria and the branch of cinnamomum cassia couldincrease myocardial nutritional blood flow in mice The Pharmacologicalactions of these drugs have heen reviewed briefly.

Objective:To study the content of polysaccharide and its antitumor activity in the edible part and the disused part of asparagus. Methods:A orthogonal design of 4 factors and 3 levels was applied to optimize the solvent concentration, solid-liquid ratio, extraction duration and temperature that influencing the extraction efficiency. The difference in the polysaccharides content between the two parts was compared, and the difference in the antitumor activity against MCF-7 cells was observed with SPSS software. Results:The optimum extraction technology was used. The difference in the polysaccharide content between the edible part and disused part of asparagus was significant (P<0. 05). The antitumor activity of the two refined polysaccharides against MCF-7 cells was without nota-ble difference (P>0. 05). Conclusion:There is significant difference in the content of polysaccharide in the edible part and the dis-used part of asparagus, however, there is no significant difference in the antitumor activity in the two parts of asparagus.

New investigations indicated that half of the people in the world had the risk of vitamin D deficiency.The main reasons for vitamin D deficiency was that people ignored the importance of using vitamin D supplement in special ages and seasons.Children were potentially at high risk for vitamin D deficiency.Vitamin D deficiency during childhood could cause rickets,growth retardation,skeletal deformities and might increase the risk of osteoporosis and hip fracture in later life.Vitamin D deficiency were also related with cancer,autoimmune disease,endocrine system disease,nervous system disease,hypertension and infection.

Objective To study the glucose-6-phosphate dehydrogenase(G6PD)genotypes in the children of viral hepatitis with G6PD deficiency and investigate the relationship between G6PD deficiency and viral hepatitis.Method DNA samples of 18 children with viral hepatitis/ G6PD deficiency were studied for the 3 common mutations by using the natural primers or mismatched primers mediated PCR followed by restriction enzyme analysis and their clinical manifestation was analyzed.Results Among 18 cases,8 cases were G1388A,and 4 cases were G1376T,and 1 case was A95G.The 3 mutations of G1388A,G1376T and A95G added up to 72.22%.There were no significant difference between the patients with viral hepatitis/ G6PD deficiency and the children only with G6PD deficiency.The incidence rates of acute hemolysis and acute renal failure in the viral hepatitis/G6PD deficiency patients were higher than those in children with G6PD deficiency only.Conclusions It is supposed that the G6PD deficiency is primary in the patients of viral hepatitis with G6PD deficiency.The children of viral hepatitis with G6PD deficiency have more severe pathogenetic conditions and more complications than the children with G6PD deficiency only.

Objective To investigate the distribution of vitamin D receptor(VDR) gene BsmI and Tru9I site gene polymorphism in children in Guangxi region.Methods Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method and DNA sequencing technology were used to analyze the VDR genotype in 268 healthy children [including 143 boys and 125 girls which aged (4.15?0.63) years] in Guangxi region.Hardy-Weinberg balance analysis was used to check up the samples.Results There were 246 cases which were bb genotgpes in BsmI site and 21 cases which were Bb genotypes and 1 case which was BB genotypes in 268 cases.The frequency of bb,Bb and BB in BsmI site were 91.79%,7.84% and 0.37%,respectively.Frequencies of b and B allelic gene were 95.71% and 4.29%;There were 12 cases which were tt genotypes in Tru9I site and 85 cases which were Tt genotypes and 171 cases which were TT genotypes in all 268 cases.tt,Tt and TT in Tru9I site were 4.48%,31.72% and 63.80%,respectively.Frequencies of t and T allelic genes were 20.34% and 79.66%.The result of PCR-RFLP was according to the result of DNA sequencing.The samples had group representation through Hardy-Weinberg balance analysis.Conclusions The polymorphism frequency and distribution of VDR gene BsmI and Tru9I site gene polymorphism of children in Guangxi region of China exhibit its own characteristics.BB and tt genotypes minority.