OBJECTIVE To explore the strategy of prevention and treatment to reduce the surgical site infection rate. METHODS Reviewing relatied literature,to analyze the main reasons of surgical site infection and commonly encountered pathogens. RESULTS The pathogens of surgical site infection included 48.0% of Gram-negative bacilli,40.8% of Gram-positive cocci,and 11.2% of fungi isolates. G-bacilli were still the main bacteria,and G+ cocci were also important in hospital infection. Moreover,fungal infection turned to be the important cause of surgical site infection after operation. It was thought that surgical site infection was concerned with the individual condition of every patient,irrationally using antibiotics and lack of caution about aseptic conception. CONCLUSIONS It is suggested the susceptible reason of infection be determined,and the prevention and treatment of infection during,before and after operation be pay especially attention to Meanwhile,the knowledge about infection control must be reinforced and propaganded.

Age-related macular degeneration ( AMD ) is one of the important eye diseases of the WHO present three big blindness, is one of the main blinding eye disease in people over the age of 50, people over the age of 65, about 2% of the disease caused by monocular blindness, as the population ages, AMD prevalence is increasing in our country. AMD with respect to its clinical manifestations can be divided into dry AMD and wet AMD, wet AMD is the most harmful for the vision of patients, at present there are many treatments for AMD ( mainly for wet age-related macular degeneration ) , mainly including laser treatment, drug therapy, surgical treatment, gene therapy, etc. The treatments of AMD would be illuminated in this article.

10.3969/j.issn.2095-4344.2013.25.018

Objective To investigate the effects of antisense oligonucleotide against miRNA-155 (AS-miRNA-155) on proliferation,apoptosis and invasion and migration abilities of human cutaneous squamous cell carcinoma cell line A431. Methods AS-miRNA-155 was transfected into human cutaneous squamous cell carcinoma A431 cells by using LipofectamineTM 2000. Blank control without transfection and transfected with non-sense sequence were used as non-sense sequence control. Quantitative real-time polymerase chain reaction (qRT-PCR) was performed to detect the expression of miRNA-155 in A431 cells. Cell proliferation was analyzed using dimethyl thiazolyldiphenyl tetrazolium (MTT) assay. Cell cycle arrest and apoptosis were studied by flow cytometry (FCM). Invasion and migration were measured by Transwell chamber assays. Results The relative expression of miRNA-155 mRNA was lower in the transfection group than that in the blank control group and the negative control group (F=634.57, P<0.01), but there was no significant difference between the blank control group and the negative control group. After 72 h transfection, the survival rate was significantly lower in the transfection group than that of the blank control group and the negative control group, and the transfection rate decreased significantly by 120 h (P<0.05). Cells of G0/G1 phase increased, Cells of S phase reduced, the overall PI value decreased in transfection group, and the apoptosis rate of A431 cells, migration and invasion of cells increased (P<0.05). There was no significant difference in G2/M cycle between transfection group, blank control group and negative control group. There were no significant differences in A431 cell apoptosis rate, cell migration and invasive ability between blank control group and negative control group. Conclusion Antisense oligonucleotide against miRNA-155 can inhibit the expression of miRNA-155, the proliferation and promote the apoptosis of human cutaneous squamous cell carcinoma A431 cells, which indicates that miRNA-155 may become a new target for the regulation of gene expression in cutaneous squamous cell carcinoma.

Objective To observe clinical effect of treating chronic obstructive pulmonary disease(COPD) in exacerbation stage with aerosol inhalation of budesonide combined with Chinese herbal enema. Methods 36 cases with COPD were randomly recurited into a control group and a treatment group. The control group was treated with routine western medicine, and the treatment group was treated with aerosol inhalation of budesonide combined with Chinese herbal enema. The clinical effect was observed in both groups, Results The total effective rate in the treatment group and the control group was 88.9% and 72.2%, respectively, showing significant difference between the two group. (P<0.05) .Conclusion The treatment of aerosol inhalation of budesonide combined with Chinese herbal enema on COPD is better than routine western medicine.

Objective To evaluate the therapeutic effect of plasmid encoding interleukin 4 (pIL-4) administered via gene-gun delivery and intradermal injection on collagen-induced arthritis (CIA). Methods IL-4 plasmid was administered by gene-gun delivery and intradermal injection to DBA/1 mice immunized with type Ⅱ collagen (CⅡ). Its effect on the development of CIA was evaluated clinically with the visual analogue scoring method for arthritis and serologically by enzyme linked immunosorbent assays (ELISA) and polymerase chain reaction. Results Treatment with IL-4 plasmid could significantly reduce the incidence and severity of CIA including a reduction in the anti-CⅡ antibody level. Gene-gun delivery had a stronger immunosuppressive effect on CIA compared with intradermal injection. As shown by in vitro stimulation assay, the speen cells from mice immunized with CⅡ and treated with IL-4 plasmid via gene-gun exhibited higher Th2 cytokine responses compared with cells treated with control plasmid after in vitro stimulation with CⅡ. Conclusion The results of this study suggest that treatment with IL-4 plasmid may constitute a new approach of cytokine gene therapy for rheumatoid arthritis.

Angiogenidiseaseof the retinare the common reasonof several visual loss.Vasculaendothelial growth facto(VEGF) playan importanrole in pathologiangiogenesis.In recenyears,the developmenof anti-VEGF therapiehatransformed the prognosiof these conditions.The treatmenof angiogenidisease had entered an erof anti-VEGF therapy,especially in the treatmenof choroidal neovascularization (CNV) caused by age-related maculadegeneration (AMD).Abreakthrough,anti-VEGF therapy changethe photodynamitherapy (PDT) based weAMD (wAMD) treatmenmode.In thipaper,we describe thaeven though relegated to background,PDstill haclinical therapeutivalue fospecial circumstanceand certain type of CNV lesion,awell ain certain retindisease.

Venous thromboembolism, which is a common thrombotic disease, is prone to become a fatal pulmonary embolism due to the lack of existing treatments.Although the anticoagulant drugs are widely used, the disease relapses easily.In recent years, there are much progress in the formation of venous thrombosis and diagnosis, and antiplatelet agents may play a certain role in the prevention of venous thrombosis.These new developments provide a new train of thought that platelets play as a breakthrough to find more effective treatments.This article summarizes the progress in the diagnosis of venous thrombosis on platelets in recent years, which discusses whether antiplatelet strategies could be applied in venous thrombosis so as to provide reference for further research.

Objective To observe the clinical curative effect of SCH 1000 combined with Tanreqing on chronic obstructive pulmonary diseases(COPD). Methods 96 patients with COPD were randomly recruited into a control group and a treatment group. The control group was treated with SCH 1000 aerosol and the treatment group was treated with SCH 1000 aerosol combined with Tanreqing injection. Results The treatment group had better clinical curative effects than the control group, with (P<0.05) .Conclusion The treatment of SCH 1000 combined with Tanreqing on COPD is effective.

BACKGROUND:Hydrophilic acrylic materials with good biocompatibility have been widely used in clinic. However, there are some problems about the biocompatibility and safety of hydrophilic acrylic intraocular lens after long-term clinical application. OBJECTIVE:To discuss the biocompatibility and stability issues and corresponding processing methods after hydrophilic acrylic intraocular lens implantation. METHODS:A computer-based search of Wanfang and PubMed database was performed for articles related to clinical application of hydrophilic acrylic intraocular lens published from 2005 to 2014. The keywords were “hydrophilic acrylic,intraocular lens” in Chinese and English, respectively. A total of 618 articles were initialy retrieved, and finaly 35 articles were included in result analysis. RESULTS AND CONCLUSION:As the hydrophilic acrylic intraocular lens is foldable, a smal incision is required for implantation and the operation is simple that cause less damage to the patients. In addition, the hydrophilic acrylic intraocular lens has poor bacterial and inflammatory cel adhesion, which leads to a low probability of infective endophthalmitis after implantation. But there is a high incidence of posterior capsule opacification as wel as some refractive errors and visual quality problems after implantation. Taken together, to solve these problems depends on the continuous improvements and updates of intraocular lens materials and designs.