Objective:To investigate the protection of acupuncture on damaged myelin sheath from the poinr of view of neurological functional recovery and morphological changes of the myelin sheath.Methods:Eighty-nine adult male Sprague-Dawley rats were randomly allocated to normal (n=5),sham-operation (n=21),model (n=21),early acupuncture (n=21) and late acupancture (n=21) groups.A model of middle cerebral artery occlusion was induced by the intraluminal filament method.Acupuncture was performed at different time points after ischemia.Neurological function and morphological changes of the myelin sheath of ischemic focus were observed by using the methods of neurological deficit scores and Pal-Weigert's myelin staining.Results:Neurological deficit scores at 2.5 hours after the procedure in the model group increased significantly (P＜0.05).qhe scores decreased somewhat as the time elapsed.The internal capsule had obvious demyelination and recovered slowly.Neurological deficit scores in rats at each time point decreased faster in the acupuncture group compared with the model group,and they decreased significantly at day 3 (P＜0.05);the extent of demyelination was significantly alleviated.Neurological deficit scores decreased faster in the early acupuncture group compared with the late acupuncture group (P＜0.05);and demyelination in the early acupuncture group seemed milder at day one.Conclusions:Early acupuncture is beneficial to remyelination and neurological functional recovery after cerebral ischemia.

Objective To discuss how to choose the method of anesthetization for childhood obstructive sleep apnea hypopnea syndrome(OSAHS).Methods 98 children with tonsillar and adenoidal hypertrophy were seleted,and were randomly divided into two groups.Tonsillectomy and adenoidectomy were performed for the group with local infiltration anesthesia,while Tonsil dissection and endoscopic-assisted adenoidectomy through nose were for the group with intravenous general anesthesia.Then blood loss,operation time,intake time,operation damage,psychological trau-ma,hospitalization fares and operative efficacy were compared.Results The group with general anesthesia lost more blood,underwent longer operations with higher cost;the group with local anesthesia had more operation damage,grea-ter psyhologieal trauma and sometimes general anesthesia was necessary for the children who didn't cooperate.But these two methods of anesthetization almost had the same operative efficacy,and the symptoms such as snoring,oral breathing and suffocating were significantly improved after surgery.Condusion Every method had own advantages and disadvantages.Local anesthesia was as effective as general anesthesia in treating childhood OSAHS.but the anes-thetization choice should vary with the situations.

Objective To investigate the effect of medial preoptic area ( mPOA) destruction on the anesthesia induced with propofol and ketamine and the role of mPOA in the mechanism of anesthesia. Methods Twenty-four SD rats weighing 250-300 g were randomly divided into two groups: NS group ( n = 12) and NMDA destruction group ( n = 12) . The animals were anesthetized with intraperitoneal (ip) pentobarbital 40 mg?kg-1. A hole was drilled in the skull fixed by a stereotactic apparatus (Narishige). 0.5?l of normal saline (NS) or N-methyl-D-aspartate (NMDA) was injected into mPOA. The rats were observed for changes in behaviour and body weight. On the 7 th day after NS or NMDA injection each group was further divided into 2 subgroups receiving either propofol 100 mg?kg-1 or ketamine 100 mg?kg-1 ip. The latency of loss of righting reflex (RL)(time from end of ip injection to loss of righting reflex) and recovery time (RT) were recorded. Results There were no significant changes in behaviour and body weight after mPOA injection in NS group; while animals in NMDA-destruction group showed increased excitability , irritability and activity and decreased appetite and sleep and significant weight loss after mPOA injection. RL was significantly longer and RT was significantly shorter after propofol/ketamine ip injection in NMDA destruction group than those in NS group. Conclusion mPOA is probably involved in anesthesia induced with propofol and ketamine.

Objective To observe the effect of endothelin-1 (ET-1) on the cell growth, adhesion, migration and expression of intercellular adhesion molecule 1 (ICAM-1) by A375 human malignant melanoma cells. Methods A375 cells were cultured in the presence of ET-1 of various concentrations (0.002, 0.02, 0.2, 2 μg/mL) for different periods. MTT method and flow cytometry were applied to detect the proliferation and ICAM-1 expression of these cells, respectively, after 24-, 48-, and 72-hour treatment. After 24-hour treatment, the cell adhesion and migration of A375 cells were assessed with cell adhesion assay and Transwell chambers, respectively. Results In the case of ET-1 from 0.002 to 0.2 μg/mL, it enhanced the proliferation, adhesion, migration of A375 cells and inhibited the expression of ICAM-1 by A375 cells in a dose dependent manner (P<0.01 or<0.05); however, for ET-1 of 2 μg/mL, the situation was the opposite. Moreover, after 24-hour culture with ET-1 of 0.2 μg/mL, the metabolic activity, cell adhesion rate, and expression of ICAM-1 peaked at 0.327±0.009, (163.31±4.05)% and 4.667±0.551, respectively. Conclusion ET-1 may enhance cellular metabolism and pigmentation by suppressing the expression of ICAM-1 and promoting the proliferation, adhesion and migration of melanoma cells.

Acceleration ; Algorithms ; Hand-Arm Vibration Syndrome ; diagnosis ; Humans ; Reference Standards ; Vibration

BACKGROUND:Under mitomycin C treatment, feeder cells appear to have restricted proliferation, but they are stil able to secret different cytokines. Non-mesenchymal stem cells from the bone marrow and secreted factors in plasma maintain the micro-environment suitable for the growth of mesenchymal stem cells that can improve the yield of mesenchymal stem cells.
OBJECTIVE:To study the biological characteristics of C57 mouse bone marrow mesenchymal stem cells isolated using a whole bone marrow adherent culture technique.
METHODS:Using the whole bone marrow adherent culture technique, purified and amplified C57 mouse bone marrow mesenchymal stem cells were harvested. cellproliferation kinetics, immune cellsurface markers, multiple differentiation potential and cellcycle were detected.
RESULTS AND CONCLUSION:Using the whole bone marrow culture, mouse bone marrow mesenchymal stem cells were harvested and capable of adhering to the plastic culture vessel. The obtained cells expressed CD45, CD105 and Sca-1, but were negative for CD34, CD33 and C-kit. The doubling time was (57.11±1.5) hours. The cells could be induced to differentiate into osteoblasts, adipocytes and chondrocytes. The cellcycle analysis showed that 64%of cells were in G 0-G 1 phase. These indicates that C57 mouse bone marrow mesenchymal stem cells isolated using a whole bone marrow adherent culture technique have biological characteristics of mesenchymal stem cells.

AIM:To study the chemical constituents of the roots of Astragalus hoantchy Franch.. METHOD:Isolation and elucidation of the chemical constituents,were conducted by chromatography and spectral evidences. RESULTS and CONCLUSION:Six steroids and four anthraquinones were isolated from the roots of A. hoantchy. Their structures were identified to be stigmastane-3,6-dione (1),5α,8α-epidioxy- (22E,24R)-ergosta-6,22-dien-3β-ol (2),stigmastane-3β,6α-diol (3),daucosterol (4),β-sitosterol (5),stigmasterol (6),chrysophanol (7),emodin (8),physion (9) and aloe-emodin (10) on the basis of spectral data and physical constants. Among them,compounds 1,2,3,7,8,9,10 were isolated from the genus Astragalus for the first time.

Objective To study the expressions of EGFR and VEGF in endometrium after danazol pretreatment and the pathological changes of endometrium. Methods A total of 60 patients with anovulartory functional bleeding were randomly divided into two groups: Danazol group and control group with 30 cases in each group. Danazol group were given transcervical resection of endometrium with danazol pretreatment, and control group without any pretreatment. The endometrium resected from uterus was sent for histological assessment, observation of the grandular quantity and stromal loose degree, and of the expressions of EGFR and VEGF in endometrium. Results The endometrium of patients who took Danazol were almost in proliferative phase(similar to early proliferative phase).The numbers of endometrial glands near the basal layer with Danazol pretreatment were lower than that in the control group; the stromal components (+～) were more compact than the contral group (～). The expression intensity and rates of EGFR and VEGF in glands were higher than those of stromal components. Danazol decreased the expression of EGFR and VEGF in endometrium compared to the control group. Conclusion Pretreatment with Danazol can suppress the prolifieration of endometrial cells, thin the endometrium,and disperse the glands, compact the stromal components, and it can also reduce the expressions of EGFR and VEGF in endometrial glands and stromal components compared with the control group.

BACKGROUND: It is indistinct that whether ketamine can exert antinociceptive effect througb influencing the transmission of nocuous information in spinal cord; Nitric oxide (NO) in spinal cord participates mainly in the formation and development of hyperalgesia, and it can also induce Fos protein expression. It is still controversal whether it contributes to the transmission and mediation of ketamine to pain signal.OBJECTIVE: To observe the response to formalin stimulation in spinal cord of the rats and the effect of ketamine.DESIGN: Balanced randomized animal trial.SETTING: Department of Anesthesiology, Affiliated Hospital of Xuzhou Medical College; Jiangsu Provincial Key Laboratory of Anesthesiology.MATERIALS: This trial was carried out in the Jiangsu Provincial Key Laboratory of Anesthesiology, Xuzhou Medical College from January to March 2000. Totally 30 Sprague-Dawley rats were chosen and balanced randomized into 6 groups: formalin group (n=6), formalin + ketamine group (n=6), ketamine +formalin group (n=6), ketamine group (n=6), formalin+normal saline group (n=3) and normal saline group (n=3). The gender ratio was the same in each group.METHODS: Formalin group:The rats were stimulated for one hour by subcutaneous injection of 0.05 volume fraction of 200 μL in the center of palm of unilateral fore-claw. Formalin +ketamine group: The rats were stimulated for 10 minutes by formalin, then for one hour by intraperitoneal injection of 100 rg/kg ketamine. Ketamine + formalin group: The rats were injected with ketamine for 10 minutes, then with formalin for one hour. Ketamine group: the same dosage of ketamine was intraperitoneally injected into the rats for one hour. Formalin + normal saline group: The rats were stimulated for 10 minutes by formalin, then intraperitoneally given 10 mL/kg normal saline for one hour. Normal saline group: the same volume of normal saline was intraperitoneally injected into the rats for one hour.MAIN OUTCOME MEASURES: ① Behavioral performance of the rats in each group. ② Spinal sections were chosen, and stained with c-fos genetic immunohistochemical and NADPH-d histochemical methods. The changes of the number of Fos-like immuno-positive neurons (FLI) and FLI/nitric oxide synthase (NOS) double-labeled neurons in the 4-layer sections (layer Ⅰ -Ⅱ ,layer Ⅲ-Ⅳ ,layerⅤ-Ⅵ ,layer Ⅶ-X )of spinal dorsal horn of the rats were observed.RESULTS: All the thirty rats entered the stage of result analysis. ① Behavioral changes: The rats of formalin group and formalin+ normal salinegroup had apparent pain response; Several minutes after injection with ketamine, righting reflex disappeared and did not recover at perfusion period.Prolonged sleep was found without obvious pain response performance. ② FLI neuron expression: A lot of FLI positive neurons were found in the spinal dorsal horn of injec tion side of the rats in the formalin group and formalin+ normal saline group, and they distributed principally in the layer Ⅰ - Ⅱ of spinal dorsal horn.The distribution in the ketamine + formalin group and formalin + ketamine group was basically similar to that in the formalin group and formalin + normal saline group, but positive neuron counts were significantly reduced (P ＜ 0.01). ③ The expression of FLI/NOS double-labeled neurons: The number of double-labeled neurons in the spinal dorsal horn layer Ⅰ - Ⅱ of the rats in the ketamine+ formalin group and formalin+ ketamine group were significantly less than that in the formalin group and formalin+normal saline group [(1±1), (1±1), (7±3), (8±3),P ＜ 0.01].CONCLUSION: Some neurons of ipsilateral corresponding spinal segments participate in the transmission and mediation of pain signal. Ketamine can suppress the activities of these neurons and exert antinociceptive effect. The antinococeptive function of ketamine may be caused by the activity depression of the NOS-positive neurons in spinal cord.