1.Effect of GRP94 expression down-regulation on proliferation abilities of human lung carcinoma A549 cells
Chinese Journal of Immunology 2015;(8):1024-1027
Objective:To investigate the effect of GRP94 gene expression down-regulation on the proliferation in human lung carcinoma A549 cells and possible mechanism.Methods:Expression of GRP94 was examined in 4 lung carcinoma cells and two lung bronchial epithelial cells by qRT-PCR and Western blot.Chemically synthesized siRNA targeting GRP94 gene and transfected into A549 cells used by Lipofectamine2000.The mRNA and protein expression levels of GRP94,c-myc and cyclinD1 were detected by qRT-PCR and Western blot.CCK8 assay and Colony formation assay were used to detect the effect of specific GRP94 siRNA on cell proliferation.Results:The levels of GRP94 expression in the four lung carcinoma cells were significantly higher than that in two lung bronchial epithelial cells (P<0.05).GRP94 siRNA significantly downregulated the levels of GRP94 mRNA and protein in A549 cells, and the downregulation evidently suppressed cell proliferation.However, The c-myc and cyclinD1 expression levels in A549 cells in siRNA-GRP94 group was significantly lower than that in siRNA-NC group (P<0.05).Conclusion:The levels of GRP94 expression in lung carcinoma cells were significantly higher than that in normal lung epithelial cells.Knockdown GRP94 expression significantly inhibit the level of lung carcinoma A549 cells proliferation,which may act via influencing the expression of c-myc,cyclinD1.
2.Ethical concerns about genetic testing in clinic practice
Chinese Journal of Laboratory Medicine 2016;39(7):481-483
Clinic genetic testing have make rapid progress in recent years , and have been widely applicated in genetic disease diagnosis , infection disease diagnosis and personalized treatment .The aim of this article is to pay attention to the ethical issues raised by genetic testing .The important ethical problems are proposed accompany with prenatal , adolescent, and adult genetic testing respectively .We should adopt responsible best ethical practices in the provision of genetic testing , promote the widely application of gene detection in clinic orderly.
3.Roles of miR-200c in tumor diagnosis,metastasis and chemoresistance
Ming LI ; Fang DENG ; Ming LI ; Fang DENG
Journal of International Oncology 2015;(8):602-604
miR-200c is a member of miR-200 family,and it plays an important role in tumor diagno-sis,metastasis and chemoresistance.miR-200c is identified as a molecular marker and prognostic indicator in cancer screening.It can inhibit epithelial-mesenchymal transition,tumor invasion and metastasis,and increase the chemosensitivity of tumor cells,but the relevant mechanism is still not fully clear.The studies of miR-200c and some factors in regulatory pathway are beneficial to promote its applications in clinical diagnosis.
4.CT portal venography manifestations of portal collateral circulation in patients with portal hypertension due to cirrhosis
Ming NI ; Weifu Lü ; Kexue DENG
Journal of Interventional Radiology 2009;18(11):823-826
Objective To analyze CT portal venography (CTPV) manifestations of portal collateral circulation in patients with cirrhosis by using a 16-detector row spiral CT scanner. Methods CTPV was performed in 36 patients with portal hypertension due to cirrhosis, the diagnosis was proved by clinical data, hepatic function findings and imaging signs. By using post-processing reconstruction technique, 3D images of portal venous system and portal collateral circulation were obtained. Results CTPV images displayed the portal venous system and its collateral circulation stereoscopically. Of 36 patients, left gastric varices were seen in 29 (80.6%), lower esophageal varices in 18 (50.0%), short gastric or posterior gastric varices in 15 (41.7%),paraesophageal varices in 9 (25.0%), gastro-renal or splenorenal shunts in 8 (22.2%), sponge-like transformation of portal vein in 7 (19.4%), paraumbilical and abdominal wall varices in 6 (16.7%), congenital cavernous in 6 (16. 7%) and paravertebral venous shunts in 4 (11.1%). Conclusion CTPV can well display the site, extent and severity of the portal collateral circulation in patients with portal hypertension due to cirrhosis, which is of great clinical importance for judging the patient's condition, for selecting therapeutic protocols and for estimating prognosis.
5.Determination of Ochratoxin A in Chinese Herbs by HPLC-MS/MS
China Pharmacist 2014;(10):1652-1654,1655
Objective:To determine ochratoxin A ( OTA ) in Chinese herbs by high performance liquid chromatography-tandem mass spectrometry method ( HPLC-MS/MS) . Methods: The samples were extracted by 80% methanol water solution and purified by immunoaffinity column. The chromatographic separation was carried out on a C18 column, the mobile phase was methanol-acetonitrile-0. 01 mol·L-1 ammonium acetate, and then OTA was detected by MS/MS in an ESI(-)-MRM mode. Results:The limit of detection was 0. 1 μg·kg-1 , the average recoveries ranged from 84. 8% to 91. 2%, and the relative standard deviations ( RSD) ranged from 3. 6% to 8. 1%(n=9). Conclusion:The method is accurate,sensitive and simple, and suitable for the determination of ochratoxin A in Chinese herbs.
6.Induction of neovascularization in pigs by recombinant adeno-associated virus 2 vector encoding human VEGF_(165 ) cDNA
Jie JIANG ; Xiaoli DENG ; Ming CHEN
Chinese Journal of Interventional Cardiology 2003;0(06):-
Objective To investigate the effect of rAAV2 VEGF 165 (recombinant adeno associated Virus 2 vector encoding VEGF 165 cDNA) in stimulating angiogenesis, its dose effect relationship in swine acute coronary occlusion model, and to search for a suitable dose for further investigation in swine myocardium ischemia model Methods Ligation of LAD (left anterior descending coronary artery) was performed in swine modles via mini thoracotomy rAAV2 VEGF 165 (experimental group) or phosphate buffer saline (control group) was injected around the infarcted region The experimental group was divided into four subgroups: according to the amount of virus genome, which were: 1?10 11 v g (virus genome), 5?10 11 v g, 1?10 12 v g and 5?10 12 v g Animals underwent angiographic evaluation of collateral development four weeks after the operation Expression of VEGF was assessed by semiquantitative RT PCR and immunohistochemical staining Results Four weeks after the operation, expression of VEGF was detected in all the four experimental subgroups and the level of expression was higher than that of the control group Capillary density was higher in both of the 1?10 12 v g and 5?10 12 v g subgroups, compared with the control Coronary angiography demonstrated higher collateral index to the occluded LAD in the groups of 5?10 11 v g,1?10 12 v g and 5?10 12 v g than that of the control Conclusion Direct injection of rAAV2 VEGF 165 can achieve effective expression of VEGF in swine acute ischemic myocardium At certain dosage, rAAV2 VEGF 165 can promote generation of blood ressels in swine acute ischemic myocardium and 1? 10 12 v g is a more suitable dosage for swine myocardium ischemia model
7.Association of the serum adiponectin concentration with coronary heart disease in Chinese Han population: a meta -analysis
Ming GONG ; Huacong DENG ; Qiang LIU
Chinese Journal of Endocrinology and Metabolism 2010;26(7):575-577
The association of serum adiponectin concentration with coronary heart disease (CHD) in Chinese Han population was evaluated. Present evidence demonstrated that the lowered serum adiponectin concentration was a susceptibility risk factor for CHD, while the precise relationship between serum adiponectin concentration and CHD in Chinese population requires further study.
8.Clinical Significance of Correlation Factors on Serum PSA Concentration
Chenyang ZHONG ; Ming LIU ; Shuming DENG
Journal of Chinese Physician 2001;0(04):-
0 05). 70 of 138 patients had serum PSA concentration remarkable elevation after prostate biopsy (P
9.Clinical Observation on 60 Cases of Functional Dyspepsia with Stagnation of Qi due to Spleen Deficiency Treated by Jianpi Xiaozhang Granule
Wen LIU ; Ming CHEN ; Jinmei DENG ;
Journal of Traditional Chinese Medicine 1993;0(05):-
0.05),and no adverse reaction was found in both groups.Conclusion:Jianpi Xiaozhang Granule and motilium have obvious and similar therapeutic effect on functional dyspepsia with stagnation of Qi due to spleen deficiency.
10.The study on the relationship between carotid atherosclerosis and the level of serum uric acid in elderly women with hypertension
Ming LUO ; Wenping YU ; Bing DENG ;
Chinese Journal of Geriatrics 1995;0(02):-
Objective To observe the relationship between the intima media thickness(IMT) and the level of serum uric acid in elderly female patients with hypertension Methods Eighty elderly female patients with hypertension were measured for carotid artery average IMT and the level of serum uric acid They were divided into the normal group, intima thicking group ,the plaque shaping group and the vascular stenosis group. In the abnormal groups, probable risk factors were analysed by multiple stepwise regression Results There were significantly differentces among these groups ( P