1.Research progress on Drynaria fortunei naringin on inflammation and bone activity.
Fang-ming YIN ; Lian-bo XIAO ; Yun ZHANG
China Journal of Orthopaedics and Traumatology 2015;28(2):182-186
Flavonoid naringin is widely distributed in various types of plants and is an important component of herbal Drynaria. In previous studies, Drynaria has been demonstrated to have inhibitory effects on inflammatory responses and bone destruction and exert anabolic effects on bone, has been widely used in the clinical treatment. Naringin, was in the stage of experimental yet. The experimental results have confirmed that naringin suppressed inflammation including arthritis by lowering the expression of inflammatory cytokines, and the mechanism can be explained as reducing the expression of NF-κB. Naringin has been shown to increase osteoblast proliferation by increasing the expression of BMP-2, inhibit osteoclast activity by reducing the expression of RANKL. In animal experimental, naringin was useful for osteoporosis, and the mechanisms are in-depth studies. Research in the field of traditional Chinese medicine and orthopedics, naringin as a explicit material structure in the components of Drynaria, has been concerned about the experimental studies, it is not only prosperity the development of traditional Chinese medicine research,but also ready for clinical studies anti-inflammatory and bone effects of naringin in the future.
Animals
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Anti-Inflammatory Agents
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pharmacology
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Bone and Bones
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drug effects
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Flavanones
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pharmacology
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Humans
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Medicine, Chinese Traditional
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Osteoblasts
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drug effects
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Osteoclasts
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drug effects
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Osteoporosis
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drug therapy
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Polypodiaceae
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chemistry
2.Culture of cryopreserved mouse spermatogonial stem cells.
Lian-jun LI ; De-xue LI ; Xue-ming ZHANG
Chinese Journal of Applied Physiology 2004;20(2):145-208
Animals
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Cell Culture Techniques
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methods
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Cells, Cultured
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Cryopreservation
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Male
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Mice
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Spermatogonia
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cytology
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Stem Cells
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cytology
3.Analysis on clinical features and treatment of herpes zoster patients hospitalized in real world.
Ling-Lian YUAN ; Lian-Xin WANG ; Yan-Ming XIE ; Wei YANG ; Zhi-Xin YANG ; Yan ZHUANG ; Yun-Bi ZHANG
China Journal of Chinese Materia Medica 2014;39(18):3469-3473
From the hospital information system (HIS) of 20 national grade III-A general hospitals, 2 960 cases of herpes zoster as the research object, analyzes the relations between the general information, syndrome of traditional Chinese medicine (TCM), western medicine combined diseases, the relationship between the solar term and the incidence of herpes zoster, and the combined use of Chinese and western medicine. Among the patients with 46-65 year old has the highest percentage of diseased; admission to general outpatient clinic is the most; the most common medical payment is medicare; combined disease such as hypertension, diabetes and coronary heart disease is more common; early treatment effect of herpes zoster is better than the sequelae; summer and autumn solar term patients is hospitalized more, TCM syndrome is damp heat of liver fire; about drugs, western medicine is the most commonly used vitamin B1 and mecobalamin, traditional Chinese medicine is the most frequently used Danhong injection, combination therapy with promoting blood circulation drugs and neurotrophic drugs. Thus, herpes zoster, more common in elderly patients, with no obvious relationship between solar term, should be early diagnosis and early treatment, often with combination of Chinese traditional and western medicine treatment.
Adolescent
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Adult
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Aged
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Aged, 80 and over
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Drugs, Chinese Herbal
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therapeutic use
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Female
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Herpes Zoster
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diagnosis
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drug therapy
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epidemiology
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Humans
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Male
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Medicine, Chinese Traditional
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methods
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Middle Aged
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Thiamine
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therapeutic use
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Vitamin B 12
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analogs & derivatives
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therapeutic use
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Young Adult
4.Residue decline dynamics and safety utilization of carbendazim in cultivation of Anoectochilus roxburghii.
Qing-Song SHAO ; Hong-Bo LIU ; Yu-Yun ZHANG ; Ai-Lian ZHANG ; Ming-Yan LI
China Journal of Chinese Materia Medica 2014;39(9):1559-1563
The paper aimed to study the residue decline dynamic and standards for safety utilization of carbendazim in roots, stems, leaves of Anoectochilus roxburghii and in growth media. Samples extracted with methanol were purified by liquid-liquid extraction and analysed by HPLC. The results showed that average rate of recovery was 82.9% - 95.7% and RSD were 2.0% - 6.3% with add of carbendazim in respectively diverse concentration, which meets inspection requirement of pesticide residue. Two kinds of dosages of carbendazim were treated, varying from recommended dosage (1.0 kg x hm(-2)) to 1.5 times recommended dosage (1.5 kg x hm(-2)). Results of two years test showed that the half-life period of carbendazim were 7.01 - 8.51 d in the growth media of A. roxburghii, 3.58 - 4.27 d in stems and 3.50 - 3.91 d in leaves, 4.93 - 5.71 d in roots. Providing max recommended residue of carbendazim in the cultivation of A. roxburghii is 0.5 mg x kg(-1), sprayed 4 times a year with the dosage of 1.0 kg x hm(-2), 28 days is proposed for the safety interval of the last pesticide application's and harvest's date.
Benzimidazoles
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metabolism
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pharmacology
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Carbamates
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metabolism
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pharmacology
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Chromatography, High Pressure Liquid
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Culture Media, Conditioned
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chemistry
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Dose-Response Relationship, Drug
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Fungicides, Industrial
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metabolism
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pharmacology
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Liquid-Liquid Extraction
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Orchidaceae
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drug effects
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metabolism
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Pesticide Residues
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analysis
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metabolism
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Plant Leaves
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drug effects
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metabolism
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Plant Roots
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drug effects
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metabolism
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Plant Stems
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drug effects
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metabolism
5.Novel methods for studies of testicular development and spermatogenesis: From 2D to 3D culture.
Lian-dong ZHANG ; He-cheng LI ; Tong-dian ZHANG ; Zi-ming WANG
National Journal of Andrology 2016;22(3):258-263
The two-dimensional model of cell culture is an important method in the study of testicular development and spermatogenesis but can not effectively mimic and regulate the testicular microenvironment and the whole process of spermatogenesis due to the lack of relevant cell factors and the disruption of a three-dimensional spatial structure. In the past 20 years, the development and optimization of the in vitro model such as testis organotypic culture and in vivo model such as testis transplantation achieved a transformation from two- to three-dimension. The maintenance and optimization of the testicular niche structure could mimic the testicular microenvironment and cell types including Leydig, Sertoli and germ cells, which showed similar biological behaviors to those in vivo. Besides, the cell suspension or tissue fragment floats in the gas-liquid interface so that the development of somatic and germ cells is well maintained in vitro whilst the feedback linkage between grafted testis tissue and hypothalamus-pituitary of the host rebuilt in the in vitro model provides an endocrinological basis for spermatogenesis, which serves as an effective methodology to better understand the organogenesis and development of the testis as well as testicular function regulation, advancing the concept of treatment of male infertility. Al- though each of the methods may have its limitations, the progress in the processing, freezing, thawing, and transplantation of cells and tissues will surely promote their clinical application and present their value in translational medicine.
Cell Culture Techniques
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Germ Cells
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physiology
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Humans
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Infertility, Male
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therapy
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Male
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Spermatogenesis
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physiology
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Testis
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growth & development
6.Experimental Study of Gout Granule f or Gout
Lian NING ; Ruomin JIN ; Yaoping WU ; Haigui ZHANG ; Haihong QI ; Ming ZHANG ; Bi LI
Traditional Chinese Drug Research & Clinical Pharmacology 1993;0(01):-
Objective To investigate the effect of Gout Gra nule(GG)for gout.Methods Gout -relieving effect of GG was observed in rats with adjuvant arthritis induced by sodium urate and i n mice with hyperuricemia induced by hypoxan-thine.Anti -inflammation of GG was i nvestigated in mice with xylene -ind uced auricular swelling and in rats with edema of hind paw induced by injection of carrageenin.WBC migratory response c aused by carboxymethylcellulose(CMC)in mice was also observed.Results GG could reduce the blood level of uric acid and inhibit acute inflammatio n in model ani-mals and also tended to promote the elimination of uric acid.Conclusion GG had a remarkable effect on the acut e attack of gout.
7.Endemic fluorosis in Jilin province: analysis of surveillance data for 2006 - 2010
Hai-tao, ZHANG ; Zhen-ming, LU ; Hong-yan, TANG ; Xiu-li, ZHANG ; Lian-ying, FANG
Chinese Journal of Endemiology 2011;30(3):298-302
Objective To identify changes in the occurrence of endemic fluorosis in order to provide scientific basis for making countermeasures. Methods Five villages from 14 counties of mild, moderate and severe fluorosis affected areas were selected by stratified cluster sampling every year in the whole province during 2006 - 2010. Water and urinary fluorine were determined by ion selective electrode method(GB/T 8538-1995); dental fluorosis of children 8-12 years old was diagnosed with Dean method; skeletal fluorosis was diagnosed according to "clinical indexing standards of endemic skeletal fluorosis "(GB 16396-1996), between 2006 and 2008, and "clinical diagnosis standard of endemic skeletal fluorosis"(WS 192-2008) between 2009 and 2010. Results A total of 25 diseased villages were surveyed, 14 with water sources changed, covered a resident population of 8005 people, beneficiary population 7154, and accounting for 89.37% of the resident population; not changed villages 11. In accordance with the "State drinking water health standards", in the 14 changed villages the fluoride in drinking water was qualified (≤ 1.20 mg/L), there were 3 schools whose water fluorine content exceeded the standard; among the 11 villages that did not change water sources 7 drinking water samples fluorine content exceeded the standard. Of the 8 to 12 years old children in villages with changed water sources, 363 of them were checked and 142 dental fluorosis were found, the detection rate of dental fluorosis was 39.12% (142/363); in villages with water sources not changed, 303 children were checked, the detection rate of dental fluorosis was 43.89%(133/303). Of sixteen and elder adults in water source changed villages, 6424 people were checked and 403 skeletal fluorosis were found, skeletal fluorosis detection rate was 6.27% (403/6424); 3572 people were checked in not changed villages, the detection rate of skeletal fluorosis was 13.89%(496/3572). In water sources changed areas, geometric mean of urinary fluoride was in the normal reference value(WS/T 256-2005, 1.40 mg/L)or less. Conclusions Endemic fluorosis is decreased in water improved areas, but in unimproved areas the disease is still severe, and control of endemic fluorosis is still an arduous task.
8.Effect of Tanreqing injection on immune activity of peripheral blood lymphocytes of patients with lung cancer.
Ming MA ; Xing-xiao YANG ; Jie ZHANG ; Lian-mei ZHAO ; Li-hua LIU ; Bao-en SHAN
China Journal of Chinese Materia Medica 2015;40(6):1207-1211
To investigate the effect of Tanreqing injection on immune activity of peripheral blood lymphocytes of patients with lung cancer. The peripheral blood lymphocytes of patients with lung cancer and healthy persons were separated by the density gradient centrifugation method for subsequent experiments, with those from healthy persons as the positive control. The effect of Tanreqing injection on stimulating the proliferation of lymphocytes with phytohemagglutinin (PHA) was determined by MTT method. The effect of Tanreqing injection on the lymphocyte secretions of IFN-γ and TNF-α and the subset ratio of lymphocytes cultured separately or with Tanreqing injection of different concentrations were examined by ELISA and flow cytometry (FCM) respectively. In addition, the LDH release assay was used to detect the cytotoxicity of cytotoxic T cells (CTL) and natural killer cells (NK). According to the findings, all of immunological indexes of lymphocytes from patients with lung cancer were weaker than that of healthy persons, but with the obvious increases in proliferation activity and IFN-γ and TNF-α secretions of lymphocytes co-cultured with Tanreqing Injection (P < 0.05). Among lymphocyte subsets co-cultured with Tanreqing Injection, CD3+, CD3+ CD4+ and CD3- CD16 + 56+ cell ratios notably increased, whereas CD4+ CD25+ Treg cell ratio obviously decreased (P < 0.05). In the meantime, Tanreqing injection can markedly promote the cytotoxicities of CTL and NK (P < 0.05). In conclusion, Tanreqing injection shows a significant effect in promoting the immune activity of lymphocytes from patients with lung cancer and their anti-tumor immunity.
Cell Proliferation
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drug effects
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Cells, Cultured
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Drugs, Chinese Herbal
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administration & dosage
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Humans
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Interferon-gamma
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genetics
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immunology
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Killer Cells, Natural
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drug effects
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immunology
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Lung Neoplasms
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drug therapy
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genetics
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immunology
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physiopathology
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T-Lymphocytes, Cytotoxic
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drug effects
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immunology
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Tumor Necrosis Factor-alpha
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genetics
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immunology
9.Outcome of intracytoplasmic sperm injection cycle:fresh compared to cryopreserved-thawed testicular and epididymal spermatozoa
Qiufang ZHANG ; Jie QIAO ; Quan BAI ; Ming LI ; Ying LIAN ; Yuqi WU ; Ping LIU
Chinese Journal of Obstetrics and Gynecology 2009;44(10):740-744
Objective To compare clinical outcome of intracytoplasmic sperm injection(ICSI)cycle by using fresh and cryopreserved-thawed testicular and epididymal spermatozoa in azoospermic patients.Methods Between September 2006 and May 2007,208 azoospermic patients underwent in vitro fertilization(IVF)were treated in Center of Reproductive Medicine,Peking University Third Hospital.Those couples were divided into two groups based on their wishes,including 171 cases in fresh group and 37 cases in cryopreserved-thawed group.The cryopreserved testicular or epididymal spermatozoa were thawed and recovered before ICSI procedure iu thawed group.The outcomes of ICSI in each group were compared.including clinical outcomes(two pronuclear fertilization,high quality embryo,clinical pregnancy and embryo implantation)and pregnancy outcomes(spontaneous miscarriage,gestational weeks and neonatal birth weight).Results (1),The utilization rate were 92%(23/25)in cryopreserved-thawed testicular spermatozoa and 100%(12/12)in epididymal spermatozoa.(2)Between fresh and cryopreserved-thawed groups,no statistical difference was observed in two pronuclear fertilization rate[62.25%(973/1563)vs.64.53%(282/437),P=0.960],high quality embryo rate[78.9%(768/973)vs.79.1%(223/282),P=0.985],clinical pregnancy rate per embryo transfer[44.4%(60/135)vs.46.9%(15/32),P:0.688]and embryo implantation rate[29.3%(84/287)vs.33.3%(23/69),P=0.508].(3)No significant difference between flesh and cryopreserved group was found in spontaneous miscarriage rate (11%vs.7%,P=1.000),gestational weeks(single birth:39.0 weeks vs.38.7 weeks,P:0.538;twins:36.8 weeks vs.36.3 weeks,P=0.571)and birth weight(single birth:3409 g vs.3350 g,P=0.699;twins:2584 g vs.2635 g,P=0.703).Conclusion It suggested that tissue from the azoospermic patients who underwent diagnostic testieular and epididymal biopsy should be eryopreseved for IVF-ET.