Objective:To investigate the injury on isolated cadaver testes induced by ischemia-reperfusion (I/R).Methods:13 isolated cadaver testes contributed by 13 persons were preserved under 0℃～4℃ hypothermia and then reperfused under 37℃.Histology and histochemical changes were observed.Results:4℃ cold ischemia in 18 hours only could induce trivial swelling and vascular degeneration of endothelial cells(ECs).Obvious pathologic changes occurred after 24 hours,including detachment of ECs,separation between basement membrane and seminiferous epitelium,malalignment of spermatogenous cell and edema of mesenchyme.Injury was worse along with the prolongation of cold preservation time.Changes of LDH and SDH activities were found by histochemical staining.Reperfusion following 6 hours ischemia induced tissue injury and unusual enzyme activity.All changes were more obvious after reperfusion following 12,18,24 or 36 hours of cold ischemia.Conclusion:HCA can preserve testes for 18 hours.Testes can't keep normal histological and histochemical structure beyond 24 hours of preservation under 4℃ hypothermia,and 37℃ reperfusion can make injury worse.

Objective To study the effects of the accessory deep peroneal nerve,an anomalous branch of the superficial peroneal nerve, on the diagnosis of the deep peroneal nerve injuries. Methods Ten cases (7 male, 3 female, mean age 32.8 years) of deep peroneal nerve injuries with the presence of accessory deep peroneal nerve were examined in this study. All the cases were reviewed and analyzed with regard to the basic anatomy, clinical symptoms, electrophysiological data and diagnosis. Results In the presence of the accessory deep peroneal nerve, the lesions of the deep peroneal nerve spare the function of the lateral portion or the whole of the the extensor digitorum brevis (EDB) muscle. It may result in an unconformity of the severity of lesion and its clinical symptoms as well as the electrophysiological findings. Conclusion Knowledge of this anomaly is of crucial importance in the clinical evaluation of nerve injuries of deep peroneal nerve, as well as in avoiding errors in interpreting the electrophysiological data of this nerve.

Objective To study the effect of the Riche-Cannieu anastomosis, an anomalous anastomosis between the deep branch of ulnar nerve and the recurrent branch of median nerve in the palm of the hand, on the diagnosis of patients with carpal tunnel syndrome. Methods Twelve patients (2 male, 10 female, mean age 49.8 years) with carpal tunnel syndrome with the presence of Riche-Cannieu anastomosis were tested in this study. Their clinical data including the symptoms and signs, electrophysiological findings and diagnosis were collected and discussed in relation to an anatomical review of these nerves. Results Due to the existence of the Riche-Cannieu anastomosis, the patients with carpal tunnel syndrome spared part or all of the function of the abductor pollicis brevis (APB) muscle. It may result in an unconformity of the patients′lesion degree caused by carpal tunnel syndrome and its clinical symptoms and electrophysiological findings. Conclusion Knowledge of the Riche-Cannieu anastomosis is of crucial importance in the clinical evaluation, diagnosis and treatment of carpal tunnel syndrome, as well as in avoiding errors in interpreting the electrophysiological data of the patients.

Objective;To investigate whether the traditional Chinese medicine Yisheng Injection can reduce the injury on isolated rat heart induced by cold ischemia. Methods ;18 isolated female closed colony SD rats were divided into two groups at random. In the control group, the isolated rat hearts were preserved in 0.9% sodium chlorine solution at 4℃ , while in the experimental group ,the hearts were preserved in 0.9% sodium chlorine solution containing Yisheng injection at 4℃. The specimens were harvested 2,6,12, 24,48 and 72 hours after preservation. Histochemical changes were observed in two groups. Results ;In the control group, the activities of alkaline phosphatase ( ALP) and NADPH diaphorase (NADPHD) decreased after cold ischemia for 24 hours. Lactic dehydro-genase (LDH) decreased slightly, while ALP and NADPHD decreased evidently, after cold ischemia for 48 hours. Succinic dehydro-genase ( SDH) and cytochrome oxidase ( CCO) decreased slightly, and NADPHD disappeared after cold ischemia for 72 hours respectively. In the experimental group, NADPHD, LDH and CCO decreased slightly after cold ischemia for 24, 48 and 72 hours. ALP decreased slightly after cold ischemia for 48 hours, however, SDH showed no changes, after cold ischemia for 72 hours. Conclusion : Cold ischemia can induce cold ischemia injury in isolated rat hearts, especially the vascular endothelial cells being more sensitive. Yisheng injection can reduce this kind of injury to some degree.

0.05),however,by the 12th week,there was a significant difference(3.6?0.48 vs 2.4?0.48,P0.05)in the graft vasculopathy between two groups.Conclusion: It is practicable to establish a cardiac chronic graft failure model between closed colony SD rats,and ischemia reperfusion injury,as an alloantigen independent factor,would accelerate the development of rat cardiac chronic graft vascular disease,which can save the time for establishing models.

Objective To investigate the clinical and electrophysiological characteristics of entrapment syndrome involving the median nerve's recurrent branch. Methods Fourteen cases (10 male, 4 female, mean age 34.7) of medial nerve recurrent branch entrapment syndrome were retrospectively analyzed in this study with regard to their clinical and electrophysiological data. Results Normal sensory conduction velocity ( SCV ) and normal amplitude of the sensory nerve action potential ( SNAP) were found, but there was a delay of the distal motor latency (DML) in all 14 cases. The compound muscle action potential (CMAP) amplitude was decreased in 11 cases, and normal motor conduction velocity (MCV) was demonstrated in 12 cases. Fibrillation potentials and/or positive sharp waves were detected in the abductor pollicis brevis muscle in 12 cases and in the opponens pollicis muscle in 14 cases. EMGs of the flexor digitorum superficialis and pronator quadratus muscle were normal in all 14 cases. Conclusion Electrophysiological examination could provide objective and reliable data for diagnosing and differentially diagnosing median nerve recurrent branch entrapment syndrome.

Objective To study the surgical effect of sylvian lateral fissure approach in the treatment of patients with hypertensive intracerebral hemorrhage on basal ganglia.Methods Sixty-two patients with hypertensive intracerebral hemorrhage on basal ganglia in our hospital from October 2012 to June 2015 were selected,and equally divided into control group and observation group.The control group was treated with temporal cortex approach surgery,and the observation group was treated with sylvian lateral fissure approach surgery.The hematoma clearance rates of different amount of bleeding,and nerve function indexes and ADL score before the treatment and at different time after the treatment of two groups were compared.Results The hematoma clearance rates of observation group with different amount of bleeding were higher than those of control group.The serum nerve function indexes were lower than those of control group.The ADL scores were better than those of control group.The above differences were statistically significant (P＜0.05).Conclusion The clinical effect of sylvian lateral fissure approach in the treatment of patients with hypertensive intracerebral hemorrhage on basal ganglia is better.The approach has an active role in the improvement of the hematoma clearance and nerve function and greater value for the patients with hypertensive intracerebral hemorrhage on basal ganglia.

Objective:To investigate the effects of adramycin on cell line of FRH-0201.Methods:Human hilar cholangiocarcinona cell line were cultured with adramycin (2?g/ml) through lasting different times,finally this cell line can survive after 72h cultured in the culture liquid with the adramycin(1?g/ml) .Then the medium was replaced with a fresh one without adramycin,and the cells were continuously cultured for 1 month.The in vitro studies included the observation of the appearance with optical microscope,MTT cell proliferation assay,analysis of the cell cycle by Flow cytometry(FACS),assays of tumor marker using en{yme-linked immunosor bent assay(ELISA),the fluorescence density of adramycin.Results:Compared to the parent FRH-0201cell line:The morphology showed typical morphological characteristics,the doubling time prolonged about 3h,the cell cycle by flow cytometry identified in G_1,G_2 and S phase of cell cycle were 40.50%,19.74%and 39.77% in trial group,and 69.83%,7.29%and 22.88% in parent group,respectively.Tumor marker has no difference between them,the fluorescence density of epirubicin descending 5.2 times.Conclusion:The adramycin can affect the cell cycle and cause the change of metabolism.

Objective To discuss the therapeutic efficacy of budesonide and compound ipratropium bromide inhalated in treatment of infant bronchiolitis.Methods From January 2005 to February 2007,150 infants with bronchiolitis were randomly divided into treated group and control group.Both the two groups were treated with comprehensive treatment and antiviral therapy;control group were treated with budesonide suspemion and compound ipratropium bromide inhalated.Clinical efficacy of both two groups was evaluated.Results Cure rate of treatment group was 91%,cure rate of control group was 71%,the clinical therapeutic efficacy were compared between the two groups,the difference of which had statistical significance.Conclusion The result of experiment indicates that the therapeutic efficacy of budesonide and compound ipratropium bromide inhalated in treatment of infant bronchiolitis is better method.

Objective To observe the therapeutic effect of Famotidine combined with Hemocoagulase treating the neonatal stress ulcer complicating upper gastrointestinal hemorrhage. Methods 72 patients of stress ulcer complicating upper gastrointestinal hemorrhage were randomly divided into two groups:study group(35 cases) were treated with Famotidine plus Hemocoagulase;control group(37 cases)were cured by Famontidine only. The different therapeutic effects were compared. Results 35 cases of study group(100%)and 28 cases of control group(75.68%) were cured successfully. Compared the excellent rate of study group and control group,the former was significantly higher than the latter(P＜0.01). Conclusion Famotidine combined with Hemocoagulase treating the neonatal stress ulcer complicating upper gastrointestinal hemorrhage has notable therapeutic effect.