Objective:To observe the clinical effect of Shutiao Decoction on patients with coronary heart disease and anxiety and depression. Methods:Sixty patients with stable coronary artery disease and anxiety and depression from January 2019 to December 2019 in Kunming Municipal Hospital of Traditional Chinese Medicine were selected. They were divided into a treatment group of 30 cases and a control group of 30 cases according to random number table method. The control group recieved Western medicine routine treatment combined with psychological intervention, the treatment group was treated with Shutiao Decoction on the basis of the control group, and the treatment course of both groups was 4 weeks. Before and after treatment, TCM syndrome score, Seattle Angina Scale (SAQ), Hamilton Anxiety Scale (HAMA) and Hamilton Depression Scale (HAMD) were evaluated. The adverse events were recorded during treatment and clinical efficacy was evaluated. Results:After treatment, the total effective rate in the treatment group was 90.0% (27/30), the control group was 73.3% (22/30), where the difference between the two groups was statistically significant ( Z=-2.515, P=0.012). After treatment, the TCM syndrome score (9.37 ± 6.47 vs. 18.37 ± 6.58; t=-5.341, P<0.01) of the treatment group was significantly lower than that of the control group. Among SAQ domains’ scores, the physical activity limitation, and the stable state of angina pectoris, angina attack in the treatment group were significantly higher than those of the control group ( t values were 2.294, 7.698, 4.677, 3.215, respectively, P<0.05 or P<0.01). The HAMA score of the treatment group was significantly lower than that of the control group (9.20 ± 5.26 vs. 12.17 ± 4.88; t=-2.266, P=0.027). The HAMD score in the treatment group was significantly lower than that of the control group (11.27 ± 5.83 vs. 15.03 ± 6.16; t=-2.433, P=0.018). During the treatment period, the incidence of adverse reactions was 3.33% (1/30) in the treatment group and 6.67% (2/30) in the control group. There was no statistically significant difference between the two groups ( χ2=0.351, P=0.554). Conclusion:Shutiao Decoction combined with western medicine routine treatment can improve patients’ TCM syndromes, relief angina pectoris, anxiety and depression.

BACKGROUND:Recently, bone tissue engineering is highlighted from the nature source. OBJECTIVE:To explore the preparation of a cuttlebone-transformed hydroxyapatite scaffold as the novel bone substitution. METHODS: As raw materials, the cuttlebone appeared to have a hydrothermal reaction with diammonium phosphate under specific conditions, and the hydrothermal products were characterized respectively by X-ray diffraction, Fourier transform infrared spectroscopy, energy-dispersive X-ray spectroscopy and scanning electron microscopy. RESULTS AND CONCLUSION:The results showed that the hydrothermal products were hydroxyapatite. The cuttlebone-transformed hydroxyapatite possessed good three-dimensional structure. Cytocompatibility of the cuttlebone-transformed hydroxyapatite with MG63 cels culturedin vitro were observed and we found the cuttlebone-transformed hydroxyapatite had no obvious effects on cellgrowth and no toxicity, which can be used as new bone tissue engineering scaffolds.

Understanding of the classification of intracerebral hemorrhage may help to better manage the patients with intracerebral hemorrhage.Selecting the appropriate diagnosis and treatment measures as well as preventive strategies for different subtypes in order to improve the prognosis of intracerebral hemorrhage and prevent the recurrence.This article reviews the present common classification methods of intracerebral hemorrhage studied and reported in the world.

Objective In order to enhance the understanding of Churg- Strauss syndrome, the clinical manifestations and the pathological features of 15 patients were reviewed. Methods The clinical and patho- logical findings of 15 patients with Churg- Strauss syndrome admitted to Peking Union Medical College Hospi- tal were retrospectively analysed. Results In 15 cases, the initial clinical manifestations were poly- mononeu- ropathy, gastrointestinal disorders and skin lesions. Peripheral eosinophilia was significant in all cases. Biopsies were taken in all cases. The typical pathological features usually demonstrated in diffiferent stage of the dis- ease. Conclusions If a patient shows a clinical manifestation of fever, skin lesions, neuropathy, abdominal pain and diarrhea, as well as eosinophilia in peripheral blood, the diagnosis of Churg- Strauss syndrome should be considered. It is necessary to take biopsies from several sites at different time.

Objective To study the gene expression of Wnt signal transduction pathway in experimental liver fibrosis and to investigate its role in liver fibrosis. Methods Liver fibrosis model was induced with carbon tetrachloride in 8 SD rats. Another 8 healthy rats were served as control. The gene expression in liver tissues of models and controls were examined using real time PCR array. The differential gene expression was identified as either up- or down-regulated 2-fold. The expressions of smooth muscle actin (SMA), Wnt4, Frizzled2 and β-catenin in the tissues were examined by immunohistochemistry and Western blot. Results The examination confirmed that 36 genes were differentially expressed, including 25 genes up-regulated and 11 genes down-regulated. Compared with the controls, the expressions of Wnt4, Wnt5 a and W nt11 were up-regulated more than 13.9-, 16.5-and 2.17-fold respectively, while the expressions of Wntl and Wnt3 were down-regulated more than 2.32- and 2.15-fold respectively in fibrotic liver. Immunohistochemistry and Western blot showed that the expressions of SMA, Wnt4 and Frizzled2 in fibrotic liver were remarkably higher than those in normal controls. While the level of phosphorylated β-catenin was decreased. Conclusion Both canonical and noncanonical Wnt signal transduction pathway may involve in the mechanism of liver fibrogenesis.

Objective To investigate whether or not extracorporeal shock wave (ESW) treatment is safe for treating low back pain caused by lumbar facet joint degeneration by observing its effects on the lumbar segment of the spinal cord.Methods Five adult dogs were used for this study.ESWs were focused on their right L_(4～5) facet joints. The energy output was set at 40% of the maximum output,and a total of 1000 shocks were delivered at a frequency of 120/min.Before and 3 days after the treatment,the somatosensory evoked potentials (SEPs) induced by stimulating the tibial nerve were recorded from the animals' scalps,and the motor function of their hind limbs was evaluated with Tarlov's scale.The animals were then sacrificed and the L_(4～5) segments of their spinal cords were taken for histopatho- logical observation,with the T_(12) segments taken as controls.Results Three days after ESW treatment,the left and the right hind limbs scored 5 and 4 on the Tarlov scale.The amplitude of SEPs decreased and their latency increased. Histopathological observation showed mild swelling of the L_(4～5) segment of the spinal cord with congested veins on the surface.Erythrocyte and leukocyte exudation as well as myelin sheath swelling in the spinal cord were also observed un- der the microscope,but with no demyelination.Conclusion ESW had some adverse effects on the spinal cord when focused on the facet joint.Further studies of the safety range of ESW energy and distance are needed.

Objective To compare the effect of occupy effects of tumor in situ before surgery(OETS) and after neurosurgery (ANS) on neuroendocrine dysfunction and grading of neuroendocrine function in children with craniopharyngioma. Methods The grading evaluation criteria of neuroendocrine dysfunction in children with craniopharyngioma were drew up according to references and the endocrine feedback principle. Based on these grading evaluation criteria, the clinical date of 227 cases of children with craniopharyngioma who underwent neurosurgical treatment were retrospectively studied. These children were divided into pre-pubertal group (167 cases) and pubertal group (60 cases). The neuroendocrine impairment status before and after the surgery were evaluated separately. Results Among 227 children with craniopharyngioma, after the surgery, the incidence of the hypothalamus-pituitary-thyroid dysfunction increased from 16.74%(38/227) to 67.40%(153/227), the incidence of the hypothalamus-pituitary-adrenal gland dysfunction increased from 14.54%(33/227) to 44.49%(101/227), and the the incidence of pituitary function impairment increased from 17.62%(40/227) to 21.15%(48/227). Meanwhile, the incidence of body temperature dysregulation, sleeping disorder, personality abnormality and cognitive abnormality all increased after the surgery. The scoring and grading on neuroendocrine dysfunction in pre-pubertal group were increased after the surgery (Z=-5.20, P<0.01; Z=-4.94, P<0.01,). The scoring and grading on neuroendocrine dysfunction in pubertal group were increased after the surgery( Z=-4.10, P<0.01;Z=-4.25, P<0.01). Conclusions Both the mass effect of tumor in situ of craniopharyngioma and the neurosurgical treatment can be harmful to the neuroendocrine function. Even though the surgery can remove the mass effect of tumor in situ in the saddle area, it can increase the level of grading of neuroendocrine dysfunction. The status of neuroendocrine dysfunction can be evaluated by the grading evaluation criteria of neuroendocrine dysfunction in children with craniopharyngioma, which then provides an effective evaluation tool for the reconstruction and rehabilitation of neuroendocrine function.

Objective To explore the antitumor mechanism of xianhe yanling recipe( XHYLR ). Methods The mechanism of action of 241 compounds contained in the Chinese medicine, XHYLR was studied by using computational pharmacological. Methods including molecular docking,biotechnology network,chemical space technology and the prediction of absorption,distribution,metabolism,excretion and toxicity( ADME/T). Results Most of the compounds in XHYLR had good drug-like properties. Distribution of 241 compounds in chemical space also revealed that these compounds showed similar anti-tumor activity. It suggested that the prescription of XHYLR was reasonable. The phenomena that one molecular interacted with multiple target proteins and several molecules interact with one target protein were observed in XHYLR. For example,the COX-2 interacted with 40 micromolecules,and catechin interacted with five targets including COX-2. Conclusion These results are helpful for understanding the complicated mechanism of anti-tumor activity of XHYLR.

A total of 26 male patients with urethral condyloma acuminatum took Polystictus glycopeptide capsules orally for 4 weeks plus 1-5 sessions of photodynamic therapy.Prior to the first session,human papilloma virus (HPV) genotype of wart tissue was detected.Verrucous body fell off completely.The follow-up period was 6 months.There was one recurrent case.And 24 cases had HPV genotype DNA.The positive rate of high-risk infection was 46.2％ ; the high/low-risk mixed type cross infection was present.The multiple HPV6 low-risk infection rate was 23.1％ and multiple high-risk infection rate 58.3％ ; highrisk type HPV16,HPV31,HPV58 and HPV6,HPV11 were all of mixed types.

Objective To study the implact of tacrolimus blood concentration (Tac) after renal transplantation on blood lipid and fasting glucose (FBG) in recipients.Method The recipients who regularly given Tac + mycophenolate mofetil + prednisone after renal transplantation were chosen and those had normal preoperative levels of blood lipid and fasting glucose as subjects.The recipients were classied into high concentration group,normal concentration group and low concentration group by comparing Tac blood concentration of different postoperative periods with corresponding normal concentration range.The changes of biochemical indexes such as steady Tac valley blood concentration,blood lipid and FBG were monitored,and the differences in blood lipid and FBG levels at different periods among three concentration groups were compared.Result TG level was significantly higher (P＜0.05),and HDL-C level was significantly lower in high concentration group than in normal and low concentration groups one month after operation (P＜0.05).Three months after operation,TG and FBG levels were significantly higher in high concentration group than in normal and low concentration groups (P＜0.05 and 0.01,respectively).Six months after operation,TC,TG and FBG levels were significantly higher in high concentration group than in normal and low concentration groups (P＜0.05,0.01 and 0.05,respectively).There was no significant difference between normal and low concentration groups in various biochemical indexes at any time point (P＞0.05).Conclusion The higher blood concentration of Tac and the longer used after renal transplantation,the easier it might cause drug-induced hyperlipidemia and diabetes.