1.The Microbiology Research Progress of ANAMMOX Bacteria
Zhi-Hong ZHAO ; Xiao-Ming LI ;
Microbiology 1992;0(01):-
Anaerobic ammonium oxidation(ANAMMOX)is a new process of nitrogen conversion that has prospect most currently.The AN- AMMOX process offers great opportunities to remove ammonia from wastewater without the addition of an external carbon source and with con- siderable less aeration costs in comparison with classical methods.ANAMMOX is a biologically mediated process.Three bacteria are identi- fied responsible for the process as new deep-branching planctomycete:Brocadia,Kuenenia and Scalindua.Described it to separate and the method,biochemistry path,the ecosystem physiology characteristic for authenticate and distribute.
3.Clinical study of Basen's effect on insulin resistance in type 2 diabetic patients
Ming ZHAO ; Zhulan WANG ; Jian WANG ; Hong DU ; Xiao WANG
Journal of Medical Postgraduates 2001;14(2):121-122
Objectives:To study the effect of Basen on the sensitivity of insulin in diabetes mellitus.Methods:Blood glucose,lipoprotein,insulin,C-peptide were assayed after and before the administration of Basen.Results:Levels of insulin,blood glucose,and C-peptide were lowered after four weeks administration of Basen,which changed contrarily to ISI.Conclusions:Basen has effects not only on lowering blood glucose but also on improving the insulin resistance.
4.State of art of the radiofrequency ablation of colorectal liver metastases.
Ming ZHAO ; Jian-peng WANG ; Pei-hong WU
Chinese Journal of Oncology 2011;33(6):401-404
Antineoplastic Combined Chemotherapy Protocols
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therapeutic use
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Carcinoembryonic Antigen
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blood
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Catheter Ablation
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methods
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Colorectal Neoplasms
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pathology
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Combined Modality Therapy
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Fluorouracil
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therapeutic use
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Humans
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Leucovorin
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therapeutic use
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Liver Neoplasms
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blood
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drug therapy
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secondary
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surgery
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Neoplasm Recurrence, Local
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Organoplatinum Compounds
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therapeutic use
6.Changes of ACTH level to hypoxia in rat.
Hai-hong ZHU ; Ming ZHAO ; Pai-li GENG
Chinese Journal of Applied Physiology 2005;21(1):72-73
Adrenocorticotropic Hormone
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blood
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Animals
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Female
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Hypoxia
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metabolism
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Male
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Rats
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Rats, Wistar
7.Expression of myocardial NF-?B,iNOS and COX-2 in diabetic rats
Hong DU ; Yangtian WANG ; Ming ZHAO ; Hongjie DI ; Yanyan WANG
Chinese Journal of Pathophysiology 1986;0(01):-
AIM:To investigate the expression of nuclear factor-?B(NF-?B),inducable nitric oxide synthase(iNOS)and cyclooxygenase-2(COX-2)in diabetic myocardium.METHODS:Thirty SD rats were randomly divided into control and experimental groups.Diabetes was induced by a single intraperitoneal injection of STZ.The weight,blood glucose level and heart weight index(HWI)were measured 24 weeks after injection.The myocardial NF-?B,iNOS and COX-2 were stained at the same time.Furthermore,NF-?B activation in myocardium was investigated by electrophoretic mobility shift assay(EMSA).RESULTS:(1)Compared to the normal rats,the NF-?B-positive cells in the myocardium in diabetic rats significantly increased.(2)NF-?B activation in myocardium by EMSA was significantly higher in the diabetic rats than that in the normal rats.(3)The iNOS was not expressed in normal rat myocardium and was significantly expressed in the diabetic rat myocardium.(4)The COX-2 was rarely expressed in normal rat myocardium and was significantly expressed in the diabetic rat myocardium.CONCLUSION:The expressions of NF-?B,iNOS and COX-2 are significantly enhanced in the diabetic myocardium.
8.Study of relationship between sulfonylurea receptor gene polymorphism and type 2 diabetes
Ming ZHAO ; Hong DU ; Yanyan WANG ; Jian WANG
Journal of Medical Postgraduates 2004;0(01):-
Objective: To explore relationship between sulfonylurea receptor gene polymorphism and type 2 diabetes mellitus in Chinese subjects. Methods: Polymorphism of exon 16-3T→C in SUR gene 1 in 155 type 2 diabetic subjects and 132 controls were assessed by restriction fragment length polymorphism. Results: Frequency of “C” allele and “CC” homozygous phenotype in type 2 diabetic subjects was higher than that in control group. Conclusion:Allele “C” stands for hereditary susceptibility to diabetes.
9.GSH treatment reduces the expressions of NF-kB and inducible nitrie oxide synthase in myocardium of diabetic rats
Bin LU ; Yang-Tian WANG ; Hong-Jie DI ; Ming ZHAO ;
Chinese Journal of Endocrinology and Metabolism 1985;0(02):-
Streptozotocin-induced diabetic rats were treated with GSH for 12 weeks.The results showed that GSH significantly improved the expressions of NF-KB and inducible nitrie oxide synthase and ameliorated the myocardial tissue injury.
10.Influence of high-fluoride on thyroid function and brain damage in rats
Yan-hong, QIU ; De-ming, KONG ; Qin, YANG ; Na, ZHAO
Chinese Journal of Endemiology 2010;29(2):146-149
Objective To study the influence of high-fluoride on thyroid function and brain damage. Methods Thirty-six Wistar rats were randondy divided, according to weight and gender into 3 groups(12 rats each), i.e. control group, high fluoride group, and high fluoride plus thyroid tablet treatment group. The rats were fed with normal tap water containing no more than 5 mg/L NaF and the tap water added 100,100 mg/L NaF, respectively. After 7 months of experiment, the rats in high fluoride plus thyroid tablet treatment group were given with 0.04% thyroid tablet( 1.8 ml·kg~(-1)·d~(-1)) by gastric perfusion for three weeks. The contents of TT_3 and TT_4 in serum were detected by radio-immunological assay; the histomorphology in thyroids and brains were observed under microscopy; and the protein level of NMDAR2B subunit of glutamate receptor in the hippocampal CA1 and CA3 was measured by immunohistochemistry. Results As compared to the values of TT_3 and TT_4 in serum of rats in control group[ (0.97 ± 0.15), (84.03 ± 12.45)nmol/L], TT_3 and TT_4 in high fluoride group were obviously lower [(0.24 ± 0.07), (15.16 ± 2.08)nmol/L, all P < 0.01]; while no changes in TT_3 and TT_4 were detected in high fluoride plus thyroid tablet treatment group[ (1.02 ± 0.19), (85.63 ± 9.55)nmol/L, all P < 0.05] as compared to controls, but higher than those in high fluoride group(all P < 0.01 ). The pathological changes including partial hyperplasy, arrangement disorder, atrophy, and decreased colloid of the thyroid follicular epithelial cells in high fluoride group were observed under microscopy. In high fluoride plus thyroid tablet treatment group, the degree of the thyroid cellular hyperplasy was relatively slight as compared to high fluoride group. The swelling and disarrangement of neurons in the hippocampus were observed in high fluoride group, whereas the changes of the neurons were not so obvious in high fluoride plus thyroid tablet treatment group. The grey values of NMDAR2B positive cells in the hippocampal CA1 and CA3 in high fluoride group(167.05 ± 7.31 ) were significantly increased as compared to controls (92.53 ± 9.67 ) or high fluoride plus thyroid tablet treatment group( 101.66 ± 12.21, all P < 0.01 ). Conclusions High fluoride can induce the decreased function and changed histomorphology in thyroid and result in pathological damages in the brains of rats. However, treated with thyroid tablet to those having damages induced by high fluoride, the thyroid function and morphology can be normal, and the brain damages can be alleviated. The results indicate that hypothyroidism caused by high fluoride might be an important participating factor in brain damages caused by fluorosis.