OBJECTIVETo compare differences of clinical therapeutic effect of heat-sensitive moxibustion with different doses for irritable bowel syndrome (IBS).

METHODSSixty cases of IBS were randomly divided into a saturated-dose group (30 cases) and a traditional-dose group (30 cases). Heat-sensitive moxibustion was applied in both groups. The acupoints that had the strongest heat-sensitive feeling were selected and treated by warm and suspended moxibustion with moxa stick. Disappearance of heat-sensitive feeling was taken as the sign of treatment time in the saturated-dose group, while the traditional-dose group was treated for 15 min each time. The treatment in both groups was given twice a day for first 5 days, and from the sixth day it was given once a day for continuous 25 times, totally 30 days. Clinical symptom scores and therapeutic effect before and after treatment in two groups were observed.

RESULTSAfter the treatment, the cured and markedly effective rate was 75.0% (21/28) in the saturated-dose group, which was inferior to 44.4% (12/27) in the traditional-dose group (P < 0.05). The clinical symptom scores, including diarrhea, abdominal distension and pain, were obviously reduced in two groups compared with those before the treatment (all P < 0.05). Compared with the traditional-dose group, the symptom scores of diarrhea and abdominal distension in the saturated-dose group were obviously decreased (0.87 +/- 0.13 vs 1.27 +/- 0.21, P < 0.01; 1.12 +/- 0.41 vs 1.32 +/- 0.26, P < 0.05).

CONCLUSIONThe moxibustion featured with disappearance of heat-sensitive feeling and saturated dose has better therapeutic effect than that with traditional-dose for treatment of IBS. As individual dynamic amount of moxibustion, disappearance of heat-sensitive and quantitation varies from person to person, which is one of the key factors to obtain the best curative effect.

Acupuncture Points ; Adult ; Female ; Humans ; Irritable Bowel Syndrome ; therapy ; Male ; Middle Aged ; Moxibustion ; Treatment Outcome ; Young Adult

Myelodysplastic syndrome (MDS) is a group of heterogeneous clonal diseases characterized by ineffective hematopoiesis, peripheral blood cytopenias and high risk of transformation to acute myeloid leukemia.Recently more and more investigations indicate that the abnormality of bone marrow microenvironment is one of important reasons related to MDS. In this article the abnormality of stroma cells, cytokines and signaling pathways in hematopoietic micro-environment of MDS is reviewed.
Bone Marrow ; Cellular Microenvironment ; Cytokines ; metabolism ; Humans ; Myelodysplastic Syndromes ; Signal Transduction ; Stromal Cells ; metabolism

To discuss the hypothesis of the sensitization state of acupoints based on the original definition and the localization method of acupoint from Internal Classic as well as the clinical evidence of heat-sensitive moxibustion. Acupoints are different in the states, named resting and sensitization. The acupoints of sensitization state are the reactive sites of disease on the body surface, also the best sites for acupuncture and moxibustion treatment. The acupoints are the specific sensitive sites on the body surface relevant with diseases with the best therapeutic effects. Hence, the acupoints of sensitization state are of dynamic variability. In clinical application, only the two-step location method, preliminary and accurate localizations (sensitive probing localization) from Internal Classic can localize the acupoints precisely, rather than one-step location method such as proportional measurement or body surface landmark localization. The nature and property of acupoints are different based on the functional state, not just on the fixed locations.
Acupuncture Points ; Dyspepsia ; therapy ; Hot Temperature ; Humans ; Intervertebral Disc Displacement ; therapy ; Medicine, Chinese Traditional ; Moxibustion

Objective To investigate DNA methylation markers in the whole blood of patients with systemic lupus erythematosus(SLE),in hope to facilitate the evaluation of SLE severity.Methods Whole blood samples were obtained from 58 patients with SLE(including 14 cases of severe SLE,25 moderate SLE,19 inactive SLE)and 50 healthy controls.Bisulphite sequencing was performed to determine the methylation status of interleukin-2 common receptor gamma chain(IL-2RG)promoter region,and real-time reverse transcriptionPCR to quantify the expression level of IL-2RG mRNA,in these subjects.Results The methylation level of IL2RG promoter region was 0.217 ± 0.140,0.325 ± 0.230,0.342 ± 0.085 and 0.175 ± 0.036 in the patients withsevere,moderate and inactive SLE and healthy controls,respectively.A significant increase was observed in the methylation level of IL-2RG promoter region in the patients with inactive SLE compared with the patients with severe SLE and healthy controls(both P ＜ 0.01),and in the patients with SLE compared with the healthy controls(0.263 ± 0.047 vs.0.175 ± 0.036,P ＜ 0.05).The expression level of IL-2RG mRNA was significantly lower in the patients with SLE than in the healthy controls(2.550 ± 0.823 vs.4.293 ± 1.283,P ＜ 0.05).A negative correlation was observed between the expression level of IL-2RG mRNA and methylation level of IL2RG promoter region in 20 patients with SLE(r =-0.44,P ＜ 0.05).Conclusion The methylation status of IL2RG promoter region is statistically higher in patients with SLE than in healthy controls,and significantly different between patients with active SLE and those with stable SLE.

Objective To evaluate the automatic synthesis of 18F-labeled cyclic RGD peptide c(RGDyK)and its biological distribution in the tumor-bearing mice. Methods N-succinimidyl-4-18 F-fluorobenzoate (18F-SFB) was automatically synthesized and then re-dissolved in acetonitrile (MeCN). The cyclic RGD peptide c(RGDyK) was mixed with an hydrous DMSO and N, N-diisopropyl ethylamine (DIPEA). 18F-FBRGD was obtained by the reaction of peptide solution with 18 F-SFB. The final product was purified by HPLC gradient separation system and solid-phase extraction method. The biodistribution study and competition test of N-4-18F- fluorobenzoyl-RGD (18F-FB-RGD) in the tumor-bearing mice was performed. Results The labeling yield of 18 F-FB-RGD was (33.6 ± 3.5)%. The synthesis time was 110 min. The radiochemical purity was more than 98%. The tumor uptake of 18F-FB-RGD was (3.43 ±0.15), (2.61 ±0.14), (2.11 ±0.13), and (1.79 ±0.18) %ID/g, respectively, at 30, 60, 90 and 120 min after injection. The ratio of tumor to muscle activity ranged from 4.26 ±0.69 to 5.80 ±0.78. The tumor uptake decreased dramatically after RGD blockage. The uptake was (0.46 ±0.21) %ID/g and (2.87 ±0.59) %ID/g in the blocked and unblocked mice, respectively, at 60 min after blockage. Conclusions 18 F-FB-RGD can be automatically synthesized and it may become a promising tumor imaging agent.

Background The proliferation and migration of vascular endothelial cells is a primary link during angiogenesis.Studies showed that heat shock protein B6 (HspB6) promotes the secretion of multiple angiogenesis-related factors and therefore leads to neovascularization.Understanding the effects of neutralizing HspB6 antibody on the biological behavior of human choroidal vascular endothelial cells has an important significance in the target treatment of choroidal neovacularization diseases.Objective This study was to address the role and mechanism of neutralizing HspB6 antibody in tube formation of human choroidal vascular endothelial cells.Methods Human choroidal vascular endothelial cell line was normally cultured and harvested for total RNA extraction.Expressions of HspB6 mRNA and protein in human choroidal vascular endothelial cells were detected by reverse transcription PCR (RT-PCR) and flow cytometry (FCM).The cells were seeded on 96-well plate covered with matrigel at the density of 2×104/hole.Then the neutralizing HspB6 antibody at the concentration of 100 μg/Land 500 μg/L was added into the medium respectively,and the control cells were set without the addition of HspB6 antibody.The number of capillary tubes was calculated 12 hours after culture by three-dimensional matrigel assay.In addition,0,50,100,500 μg/L of neutralizing HspB6 antibody were added into the cell medium separately for 24hours,cell counting kit-8 (CCK-8) method was employed to assay the inhibitory rate(IR) of the cells.Transwell test was used to count the cell number across chamber membrane for the evaluation of migration ability of the cells.The apoptosis of the cells was assayed by FCM.Results Both HspB6 mRNA and protein were expressed on human choroidal vascular endothelial cells.The number of capillary tube formation of human choroidal vascular endothelial cells was (67.25±5.75),(60.39±6.41) and (39.76±10.73) /field in the 0,100 and 500 μg/L neutralizing HspB6 antibody groups,with significant difference among them (F =10.210,P =0.012),and the tube number was significantly less in the 500 μg/L neutralizing HspB6 antibody group compared with 0 μg/L neutralizing HspB6 group (P =0.005).The IR of neutralizing HspB6 antibody to the cellular proliferation and migration was enhanced with the increases of concentration and time lapse(Fconcentration =7.485,P =0.002 ; Ftime =16.684,P =0.001).The number of the cells through Transwell chamber membrane was 14.0 ± 2.5,11.1 ± 0.8,6.6 ± 0.1,6.7 ± 0.2 in the 0,50,100,500 μg/L neutralizing HspB6 antibody group respectively,and that in the 100 μg/L and 500 μg/L neutralizing HspB6 antibody group was lessened in comparison with the 0 μg/L neutralizing HspB6 antibody group(both at P=0.000).The apoptosis rate of the cells was (22.73 ± 2.53)％ in the neutralizing HspB6 antibody group,which was significantly lower than (13.33±2.08) ％ of the control group (t=4.967,P=0.008).Conclusions Neutralizing HspB6 antibody inhibits capillary tube formation of human choroidal endothelial cells in vitro in dose-and timedependent manner,probably through suppressing the proliferation and migration and promoting the apoptosis of choroidal endothelial cells.

OBJECTIVETo expound rules of acupoint heat-sensitization and its clinical application.

METHODSAnalyze and probe rules of acupoint heat-sensitization and its clinical application from the acupoint heat-sensitization phenomenon.

RESULTS(1) In the human being there are two functional states, sensitization state and rest state. When the human body has disease, acupoints on the body surface may be sensitized with various types of sensitization, and acupoint heat-sensitization is a type of acupoint sensitization. The sensitized acupoints show acupoint-specific "small stimulation inducing large response" for external relative stimulation. (2) Optimum stimulation for the heat-sensitized acupoint is moxibustion-heat, also it is the optimum point for moxibustion therapy, with a therapeutic effect far better than acupuncture and moxibustion at acupoints of routine rest state. (3) Heat-sensitized acupoints under moxibustion-heat stimulation very easily invigorates moxibustion sensation propagation along channels, greatly increasing clinical therapeutic effect.

CONCLUSIONAdvancing the concept of acupoint heat-sensitization perfects and develops the theory of acupuncture and moxibustion, "Only reaches the needling sensation to the affected area, can the needling produce effects".

Acupuncture Points ; Hot Temperature ; Humans ; Moxibustion

OBJECTIVETo expound a new viewpoint about the clinical main points of moxibustion.

METHODSStarting from study on the heat-sensitivity phenomenon of points, establish a new therapy based on the rule of heat-sensitivity of points and approach whether moxibustion can trigger the sensation propagated along the channel like acupuncture and moxibustion and whether need invigorating the sensation propagated along the channel to increase clinical effect with clinical therapeutic effects of moxibustion used as the test criterion.

RESULTSMoxibustion, just like acupuncture, can high-effectively invigorate the sensation propagated along the channel; moxibustion must trigger the moxibustion sensation propagated along the channel, can the curative effect be increased.

CONCLUSIONArrival of qi produces the effect of moxibustion.

Female ; Humans ; Infertility, Female ; therapy ; Intervertebral Disc Displacement ; therapy ; Lung Diseases, Obstructive ; therapy ; Moxibustion ; Qi ; Sensation

BACKGROUND:Nowadays,digitalization has become a major trend in the field of dentistry.It is highly important to apply 3D printing technology with the material of photosensitive resin to orthodontics,prosthodontics and so on.OBJECTIVE:To review the composition of photosensitive resin for 3D printing and its main commercial series,and to discuss about applications and prospects of photosensitive resin in the field of dentistry.METHODS:Using "3D printing,digitalization,photosensitive resin,dentistry" as key words in English and Chinese,the authors seareched the database PubMed and CNKI,respectively,for articles addressing the development of 3D printing technology,classification and composition of photosensitive resin as well as the progress and application of photosensitive resin in the field of dentistry over the past 15 years (1991-2016).RESULTS AND CONCLUSION:Because of achieving the visualization of data,having advantages such as personalized design printing,fast small batch production,efficient and free molding manufacturing and being easy to manufacture complex modeling products,3D printing technology has been developed rapidly in various areas.Light-curing rapid prototyping technology (Stereo Lithigraphy Apparatus,SLA),based on photosensitive resin,is one of the most widely used technological processes of 3D printing.Photosensitive resin for 3D printing can be classified by composition and the main commercial series.At present,the development of photosensitive resin for 3D printing meets the demand of personalized design in the field of dentistry.It has been developed rapidly in orthodontics,prosthodontics,oral implantology,and oral internal medicine.It can be used in fixed denture,implanted surgical guide,base for removable denture and fused model manufacture.With the continuous expansion of the types of materials and improvement of material performance,digital dentistry will become a major trend in the future.