To observe the preventive effects of flumeprednisolone and spongia gelatinosa on peridural adhesion after nucleus pulposus resection of lumbar intervertebral disc, 153 cases with lumbar intervertebral disc protrusion were selected from the Department of Orthopaedics, Fourth Affiliated Hospital of Guangxi Medical University between June 2002 and August 2006. Among them, the flumeprednisolone and spongia gelatinosa were put surrounding nerve root and dura mater of spinal cord in 83 cases. According to the Macnab criterion, the Lasegue symptom was effectively relieved after operation, and 96.4% of excellent and good result was achieved by periodic follow-up with good functional recovery and satisfactory surgical effect. It indicates that flumeprednisolone and spongia gelatinosa composite is effective in preventing peridural adhesion after intervertebral disc operation.

Data of 39 patients (51 knees) undergoing total knee arthroplasty (TKA) in the Department of Orthopaedics,Fourth Affiliated Hospital of Guangxi Medical University between October 2001 and March 2008,were analyzed retrospectively,including 6 males (6 knees) and 33 females (45 knees) aged 59-72 years. Among the 39 cases,there were 35 cases of osteoarthritis,2 of rheumatoid arthritis,1 of osteoarthritis and pigmented villonodular synovitis,and 1 of advanced tuberculosis. Different degrees of pain,swelling,genu valgum,genu varumand and functional impairment of knee joints were found in all cases. The prosthesis adopted included 3 knees of DePuy,10 of Stryker,3 of Link,5 of Zimmer,7 of Taiwan Unite,and 23 of Pulus (made in China). Among all the cases,47 knees were treated by posterior stabilized prosthesis,and 4 knees were treated with posterior cruciate ligament-retaining TKA. Careful medical examination and evaluation were performed to control blood sugar level before operation; the operation duration was shortened as possible. Reasonable proposals were formulated to prevent the postoperative infection and deep venous thrombosis. Clinical data were analyzed using the HSS score standard. All patients were followed up for 6-57 months. No deep infection,prosthetic loosening or death was found. Postoperatively,2 patients (2 knees) developed cutaneous necrosis at incision margin,of which 1 patient (1 knee) was cured by changing dressings,and 1 patient (1 knee) was healed after incision of skin and relaxation suture. The range-of-motion of 51 knees all exceeded 90?. The motion of knee joint were increased from 65?(32?-95?) in average preoperatively to 96?(90?-110?) after the operation. The preoperative pain of all patients disappeared instantly after operation,beside one case (2 knees) and the other had a little pain. The Harris knee joint scores were increased from 39 points (25-53 points) in average preoperatively to 91 points (82-97 points) after the operation at the final follow-up; 25 cases (34 knees) were excellent,12 cases (15 knees) were good,2 cases (2 knees) were fair and the excellent and good rate was 96%. Although TKA has a satisfactory surgical effect,associated factors should be paid more attention.

Objective To investigate the efficacy of steroid spray nose treated in child with chronic nasosi- nusitis.Methods 161 cases who were examined a patient of the 5～14 years old child of a chronicⅠtype nasosi- nusitis were divided into two groups,A group 92 cases,spray with the steroid spray nose+the complex treatment.B group 69 cases,only uses the complex treatment,a month of the course of treatment,two groups of all match with the nasal sinuses to douche.Results All cases were followed-up for at least 6 months.In A group,71 cases were cured,13 cases turned for the better,8 was invalid.Total efficient rate was 91.3 %.In 13 group,40 cases were cured, 13 cases turned for the better,16 was invalid.The efficient rate was 76.8 %.The A group obviously surpassed the B group(P

Objective In order to probe other possible mechanisms of aerobic tra ining in anti-atherosclerosis besides lipid-regulation, we observed the influenc e of aerobic training on the formation of atherosclerotic plaque, the concentrat ion of nitric oxide(NO) in serum, the content of inducible nitric oxidase (iN OS) and endothelial nitric oxidase (eNOS) in thoracic aorta in ApoE-deficient mi ce. Methods The atherosclerotic lesions, the serum NO, the con tent of thoracic aortic iNOS and eNOS by ABC immunohistochemical staining combin ed with computer image quantitative analysis technique on frozen sections were m easured after 10-week swimming training. Thoracic aorta Oil Red O (ORO) staining on the intima were als o analyzed. Results Compared with controls, aerobic training d elayed the plaque formation i n ApoE-deficient mice (P

Aim To study the effect of extracts of ginkgo biloba leaves(GbE) on apoptosis in neuronal cells. Methods Primary cerebral cultures from rat fetus were used to observe the activity of neuronal cells and to determine the release of LDH and DNA gel electrophoresis.Results GbE enhanced the activity of neuronal cells, decresed the release of LDH,and relieved the structural changes of nucleus and DNA fragmentation. Conclusion GbE can inhibit apoptosis in neuronal cells.

AimTo study the effect of extracts of ginkgo biloba leaves(GbE) on apoptosis in neuronal cells. Methods Primary cerebral cultures from rat fetus were used to observe the activity of neuronal cells and to determine the release of LDH and DNA gel electrophoresis. Results Gb E enhanced the activity of neuronal cells, decresed the release of LDH,and relieved the structural changes of nucleus and DNA fragmentation. Conclusion GbE can inhibit apoptosis in neuronal cells.

Total 406 community residents entered in fitness improvement program with “management by objectives model of health promotion” from 2009 to 2011.The fitness status was monitored by Xuhui Physical Constitution Center before and after entering the program.The results showed that the level of fitness assessment,the composite score,lung capacity and reaction time were improved with the years (P ＜ 0.01) ; the step index and sitting-and-reach test in 2010 was better than those in 2009(P ＜ 0.01) ; the grip strength in 2010 and 2011 was better than that in 2009 (P ＜ 0.01); the test of single leg-standing with closing eyes in 2011 was better than that in 2009(P ＜ 0.05).There was no difference in body mass index before and after program (P ＞ 0.05).The results indicate that physical fitness and overall health can be improved by management by objectives model for health promotion.

Juvenile idiopathic arthritis(JIA)is the most common rheumatology disease in childhood period with poor prognosis.The biological agents are newly developed drugs with features of clear therapeutic targets and fast effects.But its use in JIA is still limited,so this article focuses on the clinical use experience,timming and sideffects of the biological agents on JIA.

BackgroundRetinitis pigmentosa (RP) has the genetic and phenotype heterogeneity.To determine the disease-causing gene is a foundation of gene therapy.Objective This study was to localize the pathogenic gene and screen the gene mutation associated with Han Nationality autosomal dominant retinitis pigmentosa (ADRP) in a Chinese family.MethodsTwenty-one families enrolled this study,including 12 patients with ADRP and 9 individuals with normal phenotype.Perimetry,fundus examination,electrooculogram ( EOG ) and electroretinogram (ERG) were performed in 12 patients.Genetic linkage analysis was performed on the subjects in all known genetic loci related to ADRP with a panel of microsatellite markers.Subsequently,the mutation screening of rhodopsin gene was screened by direct DNA sequencing.This study was approved by Ethic Committee of Zhongnan Hospital of Wuhan University.Informed consent was obtained from each subject.ResultsThe fundus appearance of the proband was in accordance with the ADRP,and the EOG and ERG showed undetectable.Contractive visual field also was exhibited in the proband.Linkage analysis showed that the maximum logarithm of the odds(LOD) score reached 3.6671 at marker D3S1292 at recombination fraction θ =0.0.The results of direct DNA sequencing revealed a C→ G transversion mutation at codon 53 in exon 1 of rhodopsin gene,which resulted in a proline to arginine change (Pro53Arg) in 12 patients.However,no similar mutation was found in the unaffected members of this family.ConclusionsThe missence mutation Pro53Arg in rhodopsin gene cosegregate with the RP disease.It is determined to be a pathogenic factor of this ADRP family.

OBJECTIVE:To study the effects of rosiglitazone(RH)on body weight,FPG,FFA and other indicators in diabe-tes model rabbit. METHODS:18 rabbits were evenly randomized into control group,model group and dextran group. The latter 2 groups were given alloxan intravenously to induce diabetes model. 3 groups were given RH(0.5 mg/kg)intragastrically,and dex-tran group was additionally given dextran 40 glucose injection(5 ml/kg)intravenously,once a day,for consecutive 3 weeks. Body weight,serum level of FPG,FFA,AngⅡ and NO were determined before and after medication. RESULTS:Compared with be-fore medication,body weight of rabbits in control group were increased after medication,while the levels of FFA and AngⅡ were decreased;the levels of FPG and FFA were decreased in model group;body weight of rabbits were decreased in dextran group af-ter medication,with statistical significance (P<0.01 or P<0.05);other indicators had no statistically significant difference (P>0.05). The level of FFA in dextran group was higher than in model group after medication,with statistical significance(P<0.01). CONCLUSIONS:Rosiglitazone can lead to weight gain by a mechanism which reduce the level of FFA.