Objective:To summarize the experience of retroperitoneoscopic renal pedicle lymphatic disconnection in the management of chyluria,and to investigate a new minimally invasive treatment of chyluria. Methods:9 cases of chyluria were treated by retroperitoneoscopic renal pedicle lymphatic disconnection from Jan 2005 to Dec 2008 with 7 male ones and 2 female ones. The average age was 48 years. 8 of 9 cases obtained success and 1 of 9 cases was converted to open surgery. Operation time, blooding volume, postoperative complication and hospitalization stay, and the operative effect were observed. Results:The operation time was from 65 to 105 min,with the average of 70 min. The blooding volume was from 50 to 100ml, with the average of 60ml. Chyluria disappeared immediately after operation in all patients. Postoperative stay was from 5 to 7d and no complication happened. Conclusion:Retroperitoneoscopic renal pedicle lymphatic disconnection for chyluria is a effective and efficienl method in the management of chyluria. And it is also safe , minimally invasive , with less blood loss, fewer complications , and short hospital stay .

Objective:To evaluate the application of chromogranin A(CgA) as a marker in the diagnosis of prostate cancer. Methods:Serum CgA and serum prostate-specific antigen (PSA) were detected by immunoradiometric essay in 27 cases of prostatic carcinoma ,27 cases of benign prostate hyperplasia (BPH)and 5 cases of healthy subjects. Results:Serum CgA(98.7?69.42) in patients with prostatic carcinoma was significantly higher than that in healthy subjects(32.68?26.58) and in patient with BPH(44.7?40.86)(P

Entry of enveloped viruses into host cells requires fusion of the viral envelope with a cellular membrane. This step is mediated by viral glycoproteins that undergo a dramatic conformational change. Recent advances in structure and function of the fusion proteins of the class Ⅱ viruses, Rhabdoviruses and Herpesviruses were described. Proteomics computational analyses to locate the functional domain of fusion protein were introduced. The fusion proteins of class Ⅱ and class Ⅰ viruses differ radically in their initial structures but refold toward similar final conformation (trimer of hairpin). The Rhabdoviruses and Herpesviruses have a novel fold combining features of fusion proteins from class Ⅰ and class Ⅱ. The fusion proteins of these viruses have a different conformation change and mediate a different fusion process, therefore, the proteins belong to a novel class of fusion proteins. The potent inhibitor of virus entry should be new strategies for developing antiviral drugs.

Objective To investigate the prevalent characteristics of children with viral pneumonia in different areas and years.Methods The viral antigen of cells of nasopharyngeal secretions was detected by the method of alkaline phosphatasea anti-alkalinephhosphatase in viral pneumonia children in different areas and years.Results Among 627cases , there were 290 positive cases and 14 cases were infected by 2 viral strains. Three hundred and four positive viral strains,71 cases (23.35%) were RS virus infection, 47 cases (15.46%) were AD virus infection, 74 cases (24.34%) were FLU-A virus infection,50 cases (16.44%) were FLU-B virus infection, 47 cases (15.46%) were PIV1,3 virus infection, and 15 cases (4.93%) were PIV2 virus infection. But in different areas, the kind of the viral strain of the highest infection rate was different. The viral detection rate in autumn and winter was the highest and in summer was the lowest. Conclusions FLU-A virus is the main etiological agent of children viral pneumonia,but the main etiological agent in different areas is different. In autumn and winter, the viral infection rate is high. The chance of viral infection of lower respiratory tract of big children is reduced.

China ; epidemiology ; Epidemics ; prevention & control ; Humans ; Influenza, Human ; epidemiology ; prevention & control

Electricity ; Electrosurgery ; instrumentation ; Equipment Design ; Equipment Safety ; methods ; standards ; Guidelines as Topic ; Medical Errors ; prevention & control ; Surgical Equipment ; standards

Breast Neoplasms ; diagnosis ; therapy ; Combined Modality Therapy ; Female ; Humans ; Mammography

Objective To explore the inhibitory effects of RNAi targeting survivin to suppress the expression of survivin and to induce the apoptosis of human renal cancer cell line 786-O.Methods The target gene fragments were cloned into pcDNATM 6.2-GW/EmGFPmiR vectors,and then the recombinant eukaryotic expression vectors were transfected into 786-O cells with liposomes.The expression changes in survivin mRNA and protein were detected by RT-PCR and immunohistochemical assay,and the proliferation inhibitory rate (IR) of 786-O cells was detected by M'TT assay.Results The mRNA and protein expressions of survivin in the siRNA group were significantly inhibited by the eukaryotic expression vectors,and it also inhibited significantly the growth and proliferation of 786-O cells.Conclusion Silencing survivin gene by the RNAi technique can not only effectively decrease the expressions of survivin gene and protein,but also inhibit the cell proliferation in 786-O cells.This may provide the experimental evidence for the survivin gene as a target of gene therapy in renal cancer.

Congenital idiopathic nystagmus (CIN) is characterized by monoocular or biocular involuntary,rhythmical,repeated oscillations.CIN is often referred to congenital 'motor' nystagmus since nystagmus occurs in the absence of a clinically demonstrable defect in the visual sensory system.CIN is genetically heterogeneous,and patterns of its inheritance have been well-known to include autosomal dominant.autosomal recessive and X-linked patterns.In recent years, many different genetic loci for CIN have been mapped,and researchers have found some candidate causing-disease genes.This review focuses on the recent advances of gene mapping and candidate gene analysis for molecular research of CIN.

At present,the most commonly used methods for liver function evaluation in clinical practice is static detection represented by blood biochemical examinations,with a focus on the degree of liver injury and liver dysfunction;however,such methods have significant timitations on the reflection of liver reserve function.Indocyanine green (ICG) clearance test is commonly used for the testing of liver reserve function in clinical practice and it is a minimally invasive,simple,and quick procedure available for bedside real-time monitoring and can be repeated during a short period of time.The application value of ICG clearance test in the field of liver surgery has been widely recognized.In recent years,more and more studies have been focusing on the application of ICG clearance test in the fields of internal medicine,liver interventional therapy,critically ill patients,and drug safety assessment.This article reviews the current clinical application,related advances,and future perspectives of ICG clearance test.