1.Prospect for research on deregulation of cell proliferation and apoptosis in human gliomas.
Chinese Journal of Pathology 2005;34(9):547-549
Apoptosis
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Brain Neoplasms
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genetics
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pathology
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Cell Proliferation
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Genes, Retinoblastoma
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genetics
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Genes, erbB-1
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genetics
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Genes, p53
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genetics
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Glioma
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genetics
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pathology
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Humans
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Inhibitor of Growth Protein 1
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Intracellular Signaling Peptides and Proteins
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genetics
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Mutation
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Nuclear Proteins
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genetics
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Tumor Suppressor Proteins
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genetics
2.Quantitative determination of Ophiopogonin D in Zhe Ophiopogon japonicus(Thunb.)Ker-Gawl. and Chuan Ophiopogon japonicus(Thunb.) Ker-Gawl. by HPLC-ELSD
Jianpin YU ; Jianfeng SHAO ; Ming ZHU
Traditional Chinese Drug Research & Clinical Pharmacology 1993;0(04):-
HPLC-ELSD was used to determine the content of Ophiopogonin D in Zhe Opiopogon japonicus(Thunb.) Ker-Gawl. and Chuan Ophiopogon japonicus(Thunb.) Ker-Gawl. The chromatographic conditions were as follow: Alltima C18 column (250mm?4.6mm) and the column temperature at 30℃, drift tube temperature at 98 ℃with Gas flow rate being 2.8 L/min and acetonitrile : water (55∶45) as mobile phase with the flow rate being 1.0 mL/min. There was a good linearity (r=0.9992) within the range of 0.2565~6.4125 ?g. The average recovery rate was 102.0%and RSD=2.1%.
3.Effects of thiopentone and propofol on cerebral ischemia-reperfusion injury during pediatric open heart surgery under deep hypothermic circulatory arrest
Yu CHEN ; Ming ZHU ; Shunmin WANG
Chinese Journal of Anesthesiology 1995;0(10):-
Objective To investigate the effects of thiopentone and propofol on cerebral ischemia-reperfusion injury during open heart surgery under deep hypothermia circulatory arrest in infants and young children. Methods Twenty patients with VSD and pulmonary hypertension (13 male, 7 female), aged 3-23 months and weighing 4-11 kg were randomly divided into three groups: in group A thiopentone 5mg.kg was added in CPB machine when body temperature was reduced to 30℃ (n = 7); in group B propofol 2 mg.kg-1 was added ( n =8) and group C served as control ( n - 5). When rectal temperature was reduced to 20℃, CPB was stopped and intracardiac manipulation was started. Anesthesia was induced with midazolarn 0. 2mg.kg -1, fentanyl 20 ?g.kg1 and vecuronium 0. 1mg.kg1 . After tracheal intubation the patients were mechanically ventilated and anesthesia was maintained with O2-N2O-isoflurane and intermittent boluses of fentanyl and vecuronium infusion (70?g.kg-1.h1). Radial artery was cannulated and internal jugular vein (IJV) was retrogradely cannulated until bulb, and blood samples were taken simultaneously from artery and IJV before CPB (T1 ), during circulatory arrest (T2 ) at the beginning of reperfusion ( T3 ) and 24h after operation ( T4 ) for blood gas analysis and determination of plasma concentration of lactic acid (LA), creatine kinase-BB (CKBB) activity and neuron-specific endase (NSE). Cerebral arterial-venous O2 content difference (Ca-vO2) and cerebral O2 extraction ratio (CO2ER) were calculated. Results Plasma concentration of lactic acid was increased at T3 in all three groups and was the highest in group A. Cerebral O2 metabolism decreased at T2 in all three groups and was the lowest in group B and resumed at T4 CKBB activity was increased at T3 and NSE level was increased at T3 and T4 in all 3 groups. The increase in CKBB activity and NSE level was slightest in group B. Conclusion Propofol can protect brain from ischemia-reperfusion injury in infants and young children during open heart surgery under deep hypothermic circulatory arrest.
4.Effect of Naoxintong on nuclear transcription factor -κB, matrix metalloproteinase-9 and TNF-αin the brain of ischemic rat models
Ming YU ; Yanling LUO ; Haitao ZHU
Journal of Clinical Neurology 2016;29(6):447-450
Objective To investigate the effect of Naoxintong on nuclear transcription factor-κB ( NF-κB ) , matrix metalloproteinase-9(MMP-9)and TNF-αin the brain of ischemic rat models.Methods One hundred and ten healthy male SD rats were randomly divided into sham operated group ( n=30 ) , model control group ( n=40 ) and Naoxintong treatment group(n=40).Each group was further divided into five sub group by 0.5d, 1 d, 3 d, 5 d and 7 d after ischemia-reperfusion.The middle cerebral artery occlusion ischemia reperfusion rat models were prepared by Zea Longa method;meanwhile the MRI and the Bederson score were used to select the successful models .In order to observe the changing process of the ischemic brain tissue after given Naoxintong capsule , six rats in each group each timepoint were examined by MRI .The expression levels of protein and mRNA of NF-κB, MMP-9 and TNF-αwere detected respectively by western blot and real time PCR .Results Cerebral infarction volume of Naoxintong treatment group was significantly reduced compared with the model control group .The mRNA and protein levels of NF-κB, MMP-9 and TNF-αof the Naoxintong treatment group decreased significantly compared with the model control group (P <0.05).The mRNA and protein levels of NF-κB, MMP-9 and TNF-αhad no significant difference between the sham group and Naoxintong treatment group .Conclusion Naoxintong has a neuroprotection effect on cerebral ischemia via alleviating the inflammatory factors in the ischemic area .
5.Relationship between the expression of leukemia stem cell immunophenotype and the treatment efficacy and prognosis.
Hai-bo ZHU ; Ming-feng ZHAO ; Yu-ming LI
Chinese Journal of Hematology 2012;33(1):58-60
Adolescent
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Adult
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Aged
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Aged, 80 and over
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Child
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Female
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Humans
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Immunophenotyping
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Leukemia
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diagnosis
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immunology
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therapy
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Male
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Middle Aged
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Neoplastic Stem Cells
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immunology
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Prognosis
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Treatment Outcome
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Young Adult
6.Investigation of a New Type Two-branched Stent Graft Releasing in Experimental Canine Thoracic Aorta Simulation System
Ming YAN ; Erping XI ; Shuibo ZHU ; Jian ZHU ; Yu ZHANG
Chinese Circulation Journal 2015;(1):76-79
Objective: To investigate the feasibility for reconstructing an aortic arch by a new integrated two-branched stent graft releasing in experimental canine thoracic aorta simulation system.
Methods: A new type of integrated two-branched stent graft was developed and placed in the canine thoracic aorta, the stent graft releasing in a simulated system was conducted under X-ray guidance to monitor the operating process and to explore the feasibility for invitro sent graft releasing.
Results: The new two-branched stent graft was successfully deployed in canine thoracic aorta simulation system. The releasing process was smooth, two small stent grafts were well landing, and all stent grafts were fully expanded and properly positioned.
Conclusion: Our new two-branched stent graft could successfully reconstruct the aortic arch in experimental canine. The thoracic aorta releasing system may better and truly simulate the whole process of endovascular aortic repairing, which provides a good foundation for further animal experiments.
7.Removal of Low Concentration Formaldehyde in Indoor Air by Chemisorption Using Copper Sulfate
Yu-Ling ZHU ; Qian-Ming DU ; Can-Zhu GAO ;
Journal of Environment and Health 2007;0(09):-
Objective To research the removal of the low concentration of formaldehyde in the indoor air by using copper sulfate.Methods The low concentration of formaldehyde(10.0 mg/L)in the indoor air was determined by the way of MBTH spectrophotometry.The influence of pH,chelon and concentration on the removal of different concentration formaldehyde was investigated by the way of chemisorption.Results When pH was 11.99,12.86,13.08 and 13.42,using copper sulfate,the removal rate of 10.0 mg/L formaldehyde was 43.82%,62.75%,69.21% and 73.40% respectively.When the concentration of copper sulfate was at 3.0 g/L,5.0 g/L,7.0 g/L and 10.0 g/L,the removal rate was 51.43%,73.40%,66.36% and 62.18% respectively in the condition of pH=13.42.When used potassium sodium tartrate and EDTA as the ehelon,pH=13.42,concentration of copper sulfate was 5.0 g/L,the removal rate of 2.0 mg/L formaldehyde was 77.21% and 62.51% respectively,that of 10.0 mg/L formaldehyde was 86.54% and 73.40% respectively,that of 100.0mg/L formaldehyde was 96.71% and 91.32% respectively.Conclusion Using potassium sodium tartrate as the chelon,at pH=13.42,5.0 g/L copper sulfate can produce a good removal efficiency for indoor low level formaldehyde.
8.Current status and prospectives of proton and heavier-ion beam therapy for tumor in the digestive system.
Jin-ming YU ; Wan-qi ZHU ; Xue MENG
Chinese Journal of Gastrointestinal Surgery 2011;14(11):827-829
Particle radiotherapy using proton and heavier-ion beam was first proposed for clinical application by Robert Wilson in 1946. Compared to conventional photon radiation, proton and heavier-ion beam has significant physical advantage, and heavier-ion has unique biological characteristics. With the development of accelerator and radiation technique, it is being investigated for tumor treatment in many clinical centers. This article reviews the current status of clinical application of particle therapy using proton and heavier-ion beam in digestive system tumor.
Animals
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Digestive System Neoplasms
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therapy
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Heavy Ion Radiotherapy
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Heavy Ions
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therapeutic use
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Humans
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Protons
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therapeutic use
9.Advanced in solubilization methods of water-insoluble natural drugs.
Hui ZHONG ; Yuan ZHU ; Jiang-nan YU ; Xi-ming XU
China Journal of Chinese Materia Medica 2014;39(17):3226-3231
With the development of natural products, the research activities on the solubilization methods of water-insoluble natural products have been carried out worldwide. Big molecular weight and poor solubility of most natural active ingredients lead to a very poor oral absorption and low bioavailability, which has extremely limited their development in pharmaceutical fields and clinical application. As a result, it is necessary to find out a suitable technique to improve the solubility and enhance the oral bioavailability of insoluble natural drugs. Based on the related references published in these years, this review introduced some new techniques to improve the solubility and bioavailability of natural drugs, including prodrugs, inclusion complex, solid dispersion, cocrystals, osmotic pump, liquisolid compacts, micronization, self-microemulsifying, nanosuspensions, lipsomes, polymeric micelles and so on, and summarized the theory, characteristics, application range, application examples, problems and development direction of each technique.
Administration, Oral
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Biological Availability
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Biological Products
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administration & dosage
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chemistry
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pharmacokinetics
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Chemistry, Pharmaceutical
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methods
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trends
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Solubility
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Technology, Pharmaceutical
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methods
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trends
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Water
10.Nasal septal perforation combined with chronic invasive fungal rhinosinusitis: three cases report.
Hua-ming ZHU ; Wei-tian ZHANG ; Yu-jun ZHANG
Chinese Journal of Otorhinolaryngology Head and Neck Surgery 2012;47(8):692-693
Aged
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Humans
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Male
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Middle Aged
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Mycoses
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complications
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Nasal Septal Perforation
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complications
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microbiology
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Sinusitis
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complications
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microbiology