Gastrointestinal Tract ; physiopathology ; Humans ; Infection ; physiopathology ; Sepsis ; physiopathology ; Shock, Septic ; physiopathology

Acupuncture Points ; Adult ; Aged ; Female ; Humans ; Injections ; Male ; Middle Aged ; Ozone ; administration & dosage ; Spondylosis ; drug therapy

Juvenile idiopathic arthritis(JIA)is the most common rheumatology disease in childhood period with poor prognosis.The biological agents are newly developed drugs with features of clear therapeutic targets and fast effects.But its use in JIA is still limited,so this article focuses on the clinical use experience,timming and sideffects of the biological agents on JIA.

ObjectiveTo observe the efficacy and safety of patient-controlled intravenous analgesia (PCIA) with morphine and fentanyl after cardiac surgery.MethodsSeventy patients operated with cardiac surgery were randomly divided into morphine group (group M) and fentanyl group (group F). The beginning efficacy time of analgesia,efficacy of analgesia,patient's evaluation,heart rate,respiratory rate,mean arterial pressure,and incidence of nausea and vomiting were assessed.ResultsThere were no significant differences in efficacy and patient's evaluation between two groups. In group G,the beginning efficacy time of analgesia was significantly shorter than those in group M (P<0.05),and the times of nausea and vomiting were significant less than those in group M (P<0.05).ConclusionPCIA with fentanyl and morphine for postoperative pain relief after cardiac surgery is efficient and safe. Compared with morphine,the beginning efficacy time of fentanyl is significant shorter,and times of nausea and vomiting are little.

Acupuncture Points ; Adult ; Aged ; Female ; Humans ; Injections ; Intervertebral Disc Displacement ; drug therapy ; Lumbar Vertebrae ; drug effects ; Male ; Middle Aged ; Ozone ; administration & dosage

OBJECTIVEStudy on the infection rate,influence factors and clinical characteristic of rotavirus diarrhea in children.

METHODS634 hospitalized diarrhea children was collected from 2006 June to 2010 October. The gold immunochromatographic double-antibody sandwiched assay was used to detect the antigen of Rotavirus directly. The age of onset, incidence, clinical features and multiple organ damage and other aspects were summarized and analyzed.

RESULTS308 cases was detected positively in the 634 specimens, the positive rate was 48.6%. In 6 to 12 months old children 197 cases was detected positively, accounted for 66.3%. Rotavirus was detected all the year round and the positive rate was higher in the first quarter and the forth quarter and was 63.8% and 62. 6% respectively. 68.6% accompanied with myocardial damage, 41.2% with lower respiratory tract infection, 13.3% with liver damage, 14.9% with renal damage, 9.4% with convulsions in 4.9%, accompanied by the damage of blood system.

CONCLUSIONRotavirus is the leading cause of pediatric diarrhea the main pathogens, 6-12 months infants with the highest infection rate, the first, the fourth quarter is higher. Rotavirus infection can cause multiple organ dysfunction.

Child, Preschool ; China ; Enteritis ; diagnosis ; epidemiology ; virology ; Female ; Humans ; Incidence ; Infant ; Male ; Rotavirus ; genetics ; isolation & purification ; Rotavirus Infections ; diagnosis ; epidemiology ; virology ; Seasons

Objective As a collaborator of Beijing Institute of Medical Device Testing for value assignment of state standard ma-terial candidate Cystatin-C ,we have used the internationally accepted reference material to assign value for state standard material candidate Cystatin-C ,and help Beijing Institute of Medical Device Testing get Cystatin-C national standard material certificate . Methods According to the target value and operational procedure of international reference material ERM-DA471 ,We have tested 6 dilutions of standard material candidate Cystatin-C on calibrated Hitachi 7180 immunoassay system .Results The results demon-strate good repeatability and commutability ,and have been accepted in calculating the final value for the candidate standard materi-al ,our data has assisted Beijing Institute of Medical Device Testing in passing the criteria and obtaining Cystatin-C national standard material certificate .Conclusion Compared to the data from all participating collaborators ,our results hit right on the target value , and no significant matrix effects have been observed .

Child ; Child, Preschool ; Female ; Humans ; Infant ; Infant, Newborn ; Intracranial Hemorrhages ; etiology ; pathology ; Magnetic Resonance Imaging ; Prognosis ; Tomography, X-Ray Computed

Objective:To study the effects of ~(125)I-(α_v)ASODN on the in vitro invasive ability of heptocellular carcino-ma cell line(HepG2) through PEI-RGD-mediated receptor process. Methods: Intergrin α_v-specific antisense oligonucle-otide was labeled with ~(125)I, and PEI-RGD/~(125)I-(α_v)ASODN complex was prepared by combining ~(125)I-(α_v)ASODN with polyethyleneimine derivative PEI-RGD. PEI-RGD/~(125)I-(α_v)ASODN complex was transferred into HepG2 cells through the receptor-mediated process. The effect of PEI-RGD/~(125)I-(α_v)ASODN complex on the invasive ability of HepG2 cells was examined by Boyden chamber invasive assay. Results: (1) The labeling yield and radiochemical purity of ~(125)I-(α_v) ASODN were(73.78±4.09)% and(96.68±1.38)%, respectively, and the labeled compound had a good stability in vitro after 48 h at 37℃; (2) The ability of HepG2 cells to uptake PEI-RGD/~(125)I-(α_v)ASODN reached its peek ([12.77±0.85] % ) when PEI-RGD/~(125)I-(α_v)ASODN was at 4 μl/2 μg ([12.77±0.85] %), and then gredually decreased thereafter. So the dosage of PEI-RGD/~(125)I-(α_v)ASODN for the following experiment was chosen as 2 μl/1 μg; (3) The invasive capacity of HepG2 cells was significantly reduced in PEI-RGD/~(125)I-(α_v)ASODN group compared with those in other experiment and control groups (P <0.01 ). Conclusion: ~(125)I-(α_v)ASODN mediated by PEI-RGD can effectively inhibit the invasive capacity of HepG2 cells.