1.Analysis of Cognitive Character and Countermeasure in Children with Learning Disabilities
Journal of Applied Clinical Pediatrics 2006;0(15):-
memory/attention concentrating factor.Conclusions The intelligence structure of children with LD are imbalance.It is one of important measure to intervene LD early,so as to understand cognitive advantage and feebleness,find out cognitive objection,develop cognitive dominant item,put up education of success,bring along feeble item,and process pertinency training.
2.Construction and Expression of an Eukaryotic Recombinant Plasmid from a Hybrid Gene of Antigen for Plasmodium falciparum
Lang YAO ; Ying-Jie LI ; Ming LI ;
Chinese Journal of Parasitology and Parasitic Diseases 1987;0(02):-
Objective To construct an eukaryotic recombinant plasmid with HGFSP, a hybrid gene encoding the antigen epitopes of MSA1, MSA2, RESA and CSP in different developing stages of Plasmodium falciparum(P.f. ). Methods HGFSP was sub-cloned into an eukaryotic expression plasmid pcDNA3 from a prokaryotic recombinant plasmid pSK-HGFSP to construct the eukaryotic recombinant expression plasmid pc-HGFSP. The identified recombinant was then transfected into HepG2 cells with liposome-mediated method. The G418-selected positive cell clones were tested to identify the immunogenicity of HGFSP-expressing antigen. Results It was evidenced that HGFSP was correctly inserted into pcDNA3 by restriction enzymes map analysis. HGFSP-expressing antigen-specific fluorescent response was observed in pc-HGFSP-transfected HepG2 cells. The results of SDS-PAGE and Western-blotting showed that there was a 23 kD protein band, which can be specifically recognized by anti-sera of HGFSP-expressing protein in pc-HGFSP-transfected HepG2 cell lysis. Conclusion Pc-HGFSP, an eukaryotic recombinant plasmid encoding hybrid antigen epitopes of P.f ., was constructed successfully and the antigenicity of pc-HGFSP-expressing protein was confirmed.
3.Clinical Effects of Sodium Hyaluronate Combined with Arthroscopic Debridement on Knee Osteoarthritis
Ming YAO ; Yiyang YING ; Manyang WANG
China Pharmacist 2014;(10):1716-1718
Objective:To investigate the clinical effects of arthroscopic debridement combined with intra-articular injection of sodi-um hyaluronate in the treatment of knee osteoarthritis. Methods:One hundred and seventy-two cases with knee osteoarthritis were ran-domly divided into the treatment group and the control group. The control group received arthroscopic debridement, while the treatment group received intra-articular injection of 2. 5ml sodium hyaluronate after arthroscopic debridement. Lequence score, Lysholm score and visual analogue scale for pain were compared between the two groups before the treatment and after the 1-week and 3-month treatment, and the clinical effects were compared after the 3-month treatment as well. Results: Lequence score, Lysholm score and visual ana-logue pain scores after the treatment were lower than those before the treatment with significant differences (P <0.01) in the two groups. After the treatment,Lequence score, Lysholm score and visual analogue pain scores of the treatment group was lower than those of the control group with significant differences (P<0. 05 or 0. 01). After the 3-month treatment,the clinical efficacy of the treatment group was better than that of the control group (P<0. 05). Conclusion:Arthroscopic debridement combined with intra-articular injec-tion of sodium hyaluronate in the treatment of knee osteoarthritis has better efficacy than arthroscopic debridement alone. The combina-tion can effectively improve symptoms, which is worthy of clinical promotion.
4.Hyalinizing clear cell carcinoma.
Zhao-ming WANG ; Yi PAN ; Qing YAO ; Li-xiong YING
Chinese Journal of Pathology 2005;34(6):379-380
Adenocarcinoma, Clear Cell
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metabolism
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pathology
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surgery
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Aged
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Diagnosis, Differential
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Female
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Humans
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Keratins
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metabolism
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Male
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Mucin-1
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metabolism
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Palatal Neoplasms
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metabolism
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pathology
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surgery
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Palate, Hard
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surgery
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Tongue Neoplasms
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metabolism
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pathology
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surgery
5.Relationship between serum uric acid and carotid atherosclerosis in male patients with type 2 diabetes
Qian HUANG ; Yao XU ; Bin GAO ; Pangmin LI ; Jie MING ; Ying XING ; Qiuhe JI
Chinese Journal of Endocrinology and Metabolism 2011;27(7):566-569
Objective To investigate the relationship between the level of serum uric acid(SUA) and carotid atherosclerosis in male patients with type 2 diabetes mellitus(T2DM). Methods A collection of 579 male T2DM patients with or without carotid atherosclerosis were grouped based on quartiles of SUA. Age, SUA, smokers, duration, body mass index(BMI), blood pressure, total cholesterol(TC), triglyceride(TG), high-density lipoprotein cholesterol(HDL-C), low-density lipoprotein cholesterol(LDL-C), blood urea nitrogen(BUN), creatinine(Cr), and HbA1C were determined in all subjects. The plaques in carotid arteries and intima-media thickness(IMT) were measured with high-resolution ultrasound. Results BMI, systolic and diastolic blood pressures, TG, and Cr showed a gradual increase, while HDL-C and HbA1C showed a gradual decrease according to the higher SUA quartiles in male T2DM(P<0.05). Nevertheless, the detectable rates of smokers, duration, age, TC, LDL-C, and BUN had no relationships with the SUA quartiles(P>0.05). The detectable rate of carotid atherosclerosis and the thickness of carotid plaque were positively associated with the levels of SUA in male patients with T2DM(P<0.05). However, intima-media thickness of carotid arteries did not illustrate the correlation with the levels of SUA in male T2DM patients(P>0.05). Age, HbA1C, and SUA were independent factors of carotid atherosclerosis in these patients by logistic regression(P<0.05). Conclusion The levels of SUA seems to be associated with the occurrence and development of carotid atherosclerosis in male patients with T2DM.
6.Effects of δ-optoid receptor agonist on hemodynamics and oxygen metabolism in rats with sepsis
Wenming FENG ; Ying BAO ; Ming ZHU ; Xiaohong WEN ; Maoyun FEI ; Xiaolan ZHANG ; Xiaoying JIANG ; Yao WANG
Chinese Journal of Anesthesiology 2011;31(4):491-493
Objective To investigate the effects of δ-opioid receptor agonist DADLE (D-Ala2-D-Leu5-enkephalin) on the hemodynamics and oxygen metabolism in rats with sepsis. Methods Eighty healthy male SD rats were randomly divided into 4 groups ( n = 20 each) : sham operation group (group S), sepsis group (group SEP) ,DADLE, group and DADLE2, group. Sepsis was induced by cecum ligation and puncture (CLP) in SEP, DADLE,and DADLE2 groups. In DADLE1 and DADLE2 groups, 0.5 mg/ml DADLE 10 mg/kg was injected intraperitoneally (IP) 0.5 h before CLP and immediately after CLP respectively. Mean arterial pressure (MAP), left ventricular systolic pressure (LVSP) and ± dp/dtmax were recorded at 0, 2, 4 and 6 h after CLP (T1-4). Blood samples from left common carotid artery and right external jugular vein were collected at T4 for blood gas analysis. The cardiac index (CI), O2 delivery (DO2), O2 consumption (VO2) and O2 extraction rate (ERO2) were calculated.Results Compared with group S, MAP and LVSP were significantly increased at T2, while decreased at T3,4, and ± dp/dtmax was significantly increased in group SEP, MAP was significantly increased at T2, while decreased at T3,4, LVSP was significantly increased at T2,3, while decreased at T4 , and ± dp/dtmax was significantly increased in DADLE, and DADLE2 groups, and CI, DO2 and VO2 were significantly decreased and ERO2 was increased in SEP, DADLE, and DADLE2 groups (P<0.05). Compared with group SEP, MAP, LVSP and ± dp/dtmax at T3,4 and CI, DO2 and VO2 were significantly increased, while ERO2 was significantly decreased in DADLE1 and DADLE2 groups (P < 0.05). There was no significant difference in the parameters mentioned above between DADLE1 and DADLE2 groups ( P > 0.05). Conclusion δ-opioid receptor agonist DADLE can obviously improve the hemodynamics and oxygen metabolism in septic rats.
7.Urban PM2.5 in Guangzhou induces apoptosis of PC-12 cells
Xinshuo LU ; Yan LI ; Fang WANG ; Fangfang LIU ; Yao JIN ; Ying WANG ; Weiqiang CHEN ; Ming LI
The Journal of Practical Medicine 2017;33(4):520-523
Objective To investigate the toxicity of urban PM2.5 in Guangzhou on PC-12 cells.Methods PM2.5 was collected in Guangzhou city.PC-12 cells were cultured in vitro and divided into several groups,including the control group,PM2.5 groups with different concentrations and NAC pretreated group (pretreaed with 10 μmol/mL NAC followed with 100 μg/mL PM2.5 exposure).Cells were incubated with indicated stimulator for 24 h,then cell viability was checked with cell counting Kit-8 assay,the level of intracellular ROS was labeled using H2DCFDA fluorescence probe,cell apoptosis was measured by the flow cytometry and the expression of apoptosis-related proteins,including Cytochrome C,Caspase 9,Caspase 3 and PARP,were detected by Western Blot assay.Results PM2.5 has a strong toxicity on PC-12 cells when its concentration is over 25 μg/mL.After exposure for 24 h,the cell viability was markedly decreased.The results of flow cytometry and Western blot assay showed that PM2.5 enhanced the apoptosis of PC-12 cells with the upregulatios of Cytochrome C,Caspase 9,Caspase 3 and PARP.Pretreatment with NAC could significantly diminish PM2.5-induced PC-12 cell toxicity,decreased ROS generation and apoptosis of PC-12 cells,with the down regulations of apoptosis-related proteins.Conclusion PM2.5 can cause apoptosis of PC-12 cells by inducing oxidative stress,upregulating the Cytochrome C expression and activating Caspase9/3,which may be one of the mechanisms underlying PM2.5-induced neurotoxicity.
8.The features of solitary pulmonary nodules of adenocarcinoma on 18F-FDG PET/CT
Cong-xia, CHEN ; Wen-chan, LI ; Fu-geng, LIU ; Zhi-ming, YAO ; Wan-ying, QU
Chinese Journal of Nuclear Medicine 2011;31(6):382-385
ObjectiveTo study the imaging characteristics of SPN of adenocarcinoma (ASPNs) on 18F-FDG PET/CT.MethodsThe morphological and metabolic features of 35 ASPNs on FDG PET/CT were retrospectively reviewed.SUVmax (SUV) was measured and ΔSUVmax was calculated according to ΔSUVmax =(SUVmax on delay imaging - SUVmax on early imaging)/SUVmax on early imaging × 100%.Statistical analysis was performed by software SPSS 11.5 using t-test,analysis of variance and Fisher exact test.Results( 1 ) Fifteen ASPNs (42.86%,15/35) presented as nodular pattern on FDG PET imaging,while 20 (57.14%,20/35) as lamellar,cloudy or ill-defined patterns.The SUVmax of these ASPNs followed a descending order of nodular,lamellar,cloudy and ill:defined on both early and delay imaging (F =30.696 and 24.758,both P<0.001).(2)There were 54.29% (19/35) ASPNs with SUVmax ≥2.5 and 45.71% (16/35) ASPNs with SUVmax <2.5.(3) Of 35 ASPNs,24(68.57% ) were solid nodules and 11(31.43%) were ground glass nodules with SUVmax =4.54 ±2.69 and 1.30±0.87,respectively (t =-5.234,P <O.001 ).(4) The SUVmax of ASPNs on delay FDG imaging (4.22 ±3.52) was significantly higher than that on early imaging (3.49 ±2.72) (t =-4.021,P <0.001 ).However,SUVmax was dependent on SUVmax on the early imaging:when SUVmax ≥2.5,ΔSUVmax was positive in 94.74% (18/19) of ASPNs; while SUVmax <2.5,ΔSUVmax was positive in 56.25% (9/16) of ASPNs (P =0.013).(5) Of 31 ASPNs with cell differentiation data,there were 10/17 well-differentiated ASPNs and 13/14 poorly-differentiated ASPNs with positive ΔSUVmax ( P =0.045 ).The average SUVmax of well-differentiated ASPNs was significantly lower than that of poorly-differentiated ASPNs ( 1.70 ± 1.51 vs 4.91 ± 2.69,t =- 3.951,P < 0.001 ).Conclusions The morphological and metabolic features of ASPNs are diversified.It is common for ASPN to present with SUVmax < 2.5.ΔSUVmax may be helpful for differentiating malignant from benign SPNs.
9.Effect of Magnesium Sulfate, Nifedipine Tablet Combined Salvia Injection on ET-1/NO, TXA2/PGI2 and Hemorheology of Preeclampsia Patients.
Xiao-ying ZHENG ; Jing YAO ; Jia-mei ZHU ; Mei LI ; Shu-qiong QIU ; Zhao-xia ZHU ; Ming ZHANG
Chinese Journal of Integrated Traditional and Western Medicine 2015;35(8):962-965
OBJECTIVETo observe the effect of magnesium sulfate, Nifedipine Tablet (NT) combined Salvia Injection (SI) on endothelin-1 (ET-1), nitric oxide (NO), thromboxane A2(TXA2), prostacyclin I2(PG2), and hemorheology of preeclampsia patients.
METHODSTotally 704 preeclampsia patients were randomly assigned to the treatment group and the control group, 352 cases in each group. All patients were treated with magnesium sulfate combined NT (on the first day: slow intravenous injection of magnesium sulfate 5 g + intravenous dripping of magnesium sulfate injection 10 g + oral administration of NT 30 mg; on the second and third day, intravenous dripping of magnesium sulfate injection 10 g + oral administration of NT 30 mg), while those in the treatment group were dripped with SI additionally at 20 mL per day for 3 consecutive days. Before and after treatment plasma levels of endothelin-1 (ET-1), nitric oxide (NO), TXA2, PGi2, and hemorheology indicators [such as high blood viscosity (HBV), low blood viscosity (LBV), plasma viscosity (PV), erythrocyte rigidity index (ERI), fibrinogen (FIB)] of two groups were detected.
RESULTSCompared with the same group before treatment, serum levels of ET-1, TXA2, HBV, LBV, PV, ERI, and FIB decreased in the two groups after treatment (P <0. 05), but levels of NO and PG2 increased (P <0. 05). Compared with the control group in the same period, levels of ET-1, TXA2, HBV, LBV, PV, ERI, and FIB decreased in the treatment group after treatment (P <0. 05), but levels of NO and PGI2 increased (P <0. 05).
CONCLUSIONMagnesium sulfate, NT combined SI could effectively regulate the balance of ET-1/NO and TXA2/PGI2, and improve hemorheology of preeclampsia patients.
Drug Therapy, Combination ; Drugs, Chinese Herbal ; pharmacology ; therapeutic use ; Endothelin-1 ; metabolism ; Epoprostenol ; metabolism ; Female ; Hemorheology ; Humans ; Injections ; Magnesium Sulfate ; administration & dosage ; pharmacology ; therapeutic use ; Nifedipine ; administration & dosage ; pharmacology ; therapeutic use ; Nitric Oxide ; metabolism ; Pre-Eclampsia ; drug therapy ; Pregnancy ; Salvia ; Tablets ; Thromboxane A2 ; metabolism
10.Improvement of transcriptional activity of hTERT promoter by SV40 enhancer.
Wei-ming ZHANG ; Li-ying XUE ; Yao XU ; Jun XING ; Xin GENG ; Dong WANG ; Yan-yun LI
Chinese Journal of Pathology 2006;35(11):691-693
Base Sequence
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Cell Line, Tumor
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Enhancer Elements, Genetic
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genetics
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HT29 Cells
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Humans
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Luciferases
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genetics
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metabolism
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Molecular Sequence Data
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Plasmids
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genetics
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Polymerase Chain Reaction
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Promoter Regions, Genetic
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genetics
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Recombinant Fusion Proteins
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genetics
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metabolism
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Simian virus 40
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genetics
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Telomerase
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genetics
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Transcription, Genetic
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Transfection