1.Significance of combined detection of plasma RASSF1A and p16 gene methylation in diagnosis of non-small cell lung cancers.
Gui-zhi LIU ; Yi-ming WU ; Ji-yao YANG
Chinese Journal of Oncology 2007;29(8):613-614
Adenocarcinoma
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diagnosis
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genetics
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Carcinoma, Non-Small-Cell Lung
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diagnosis
;
genetics
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Carcinoma, Squamous Cell
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diagnosis
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genetics
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Cyclin-Dependent Kinase Inhibitor p16
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blood
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metabolism
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DNA Methylation
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Genes, p16
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Humans
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Lung Neoplasms
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diagnosis
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genetics
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Tumor Suppressor Proteins
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blood
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metabolism
3.One case of secretory breast cancer
Caiping CHEN ; Wanxin WU ; Ming YAO ; Longsheng XU
Chinese Journal of Endocrine Surgery 2017;11(2):166-167
5.Targeting therapy of magnetic doxorubicin stealth liposome on human gastric cancer xenografts in nude mice
Yishu LIU ; Liqing YAO ; Pinghong ZHOU ; Xizhong SHEN ; Weiyue LU ; Ming YAO ; Zhijiang WU ; Yajun SUN
Chinese Journal of General Surgery 1993;0(03):-
Objective To study the effect of magnetic doxorubicin stealth liposome on human gastric cancer xenografts in nude mice.Methods Human gastric cancer cell line MKN-45 was implanted into 36 nude mice.Different kinds of drug were injected through the caudal vein of tumor bearing nude mice divided into 6 groups .Permanent magnet was put into tumor in targeting group.Results The growth speed of tumor in the group of MDL (+) significantaly slowed down than other groups.The rate of tumor restrain in tumor weight and tumor volume of MDL (+) group were 71% and 70%, which were remarkably higher than those of the DOX and MDL (-) group (all P
6.Distribution laws of 5 compounds in rhizome and root of Polygonum cuspidate.
Yao-wut LIU ; Jun WANG ; Shan-shan CHU ; Ming-en CHENG ; Cheng-wu FANG
China Journal of Chinese Materia Medica 2015;40(24):4834-4839
To understand the distribution and accumulation rules of polydatin, resveratrol, anthraglycoside B, emodin and physicion in different tissue structure of rhizome and root of Polygonum cospidatum, the content of 5 active compounds were analyzed simultaneously by HPLC, based on plant anatomy and histochemistry. The rhizome and root consist of different tissues, with an increased diameter, the proportions of the secondary xylem and phloem have increased. Resveratrol and polydatin mainly distributed in the pith, the secondary phloem and periderm of rhizome, and the secondary phloem and periderm of the root, while emodin and anthraglycoside B concentrated in the secondary structure and pith of rhizome mostly. In different thickness of the measured samples, the total contents of 5 compounds were correspondingly higher in thinner rhizome and root than those in the coarse ones.
Plant Roots
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chemistry
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Polygonum
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chemistry
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Rhizome
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chemistry
7.Iincidence of postoperative delirium after hip surgery in elderly patients: a meta-analysis.
Yao-jun WU ; Qing-jiang PANG ; Jiang-tao LIU ; Shuai CAO ; Yue-ming HU
China Journal of Orthopaedics and Traumatology 2015;28(12):1156-1161
OBJECTIVETo evaluate incidence of postoperative delirium after hip surgery in elderly patients by meta-analysis.
METHODSFrom January 1, 2014 to December 31, 2013, clinical literatures about postoperative delirium after hip surgery in elderly patients,were searched from the Pubmed. Literature extract table were formed according to inclusion and exclusion criteria. Stata-12.0 was applied for Meta-analysis. P was used to test heterogeneity of study, random-effect model was performed when I2 > 50%. Subgroup analysis was used according to stage of age, assessment scale of delirium and statistical area of literature. Begg test was used to test publication bias.
RESULTSTwenty-one literatures were included. Incidence of postoperative delirium after hip surgery in elderly patients by weighted and combination was 17% [95% CI (16%, 18%)]. Incidence of postoperative delirium after optional hip surgery was decreased more than emergency operation in included 5 literatures [OR = 0.32, 95% CI (0.22, 0.45)]. Incidence of postoperative delirium in patients less than 80 years old was 21% [95% CI (19%, 23%)], while 21% [95% CI (19%, 24%)] in patients more than 80 years old. Incidence of postoperative delirium in CAM evaluation scale was 23% [95% CI (21%, 26%)], while 19% [95% CI (17%, 21%)] in other evaluation scales. Incidence of postoperative delirium in Asian area was 17% [95% CI (15%, 20%)], while 23% [95% CI (21%, 25%)] in European and American area. There was no publication bias tested by Begg test (P < 0.05).
CONCLUSIONIncidence of postoperative delirium after hip surgery in elderly patients increases higher, especially in emergency operation. A standardizing research method is benefit for evaluate incidence of postoperative delirium after hip surgery in elderly patients, decreasing heterogeneity and publication bias.
Aged ; Delirium ; epidemiology ; Hip Fractures ; surgery ; Humans ; Incidence ; Postoperative Complications ; epidemiology ; Publication Bias
8.Study on the susceptible factors of atherosclerotic stenosis in proximal of the coronary artery myocardial bridges
Jian CHEN ; Ping LI ; Ming LIU ; Guang YAO ; Zhengdong WANG ; Peng WU
Chinese Journal of Postgraduates of Medicine 2009;32(28):6-9
Objective To study the susceptible factors of atherosclerotic stenosis before the coronary artery myocardial bridges.Methods The data from 88 myocardial bridge cases which received coronary angiography were statistically analyzed.Sixty-seven cages which suffered from atherosclerofic stenesis in proximal of the myocardial bridges were recruited into group A,and the other 21 cases which suffered no atherosclerotic stenosis or from atherosclerotic stenosis in distal of the myocardial bridges were mcmited into group B.Difference of the age,gender,length of myocardial bridge,systolic blood pressure (SBP),diastolic blood pressure (DBP),pulse pressure (PP),the oppression degree of myocardial bridge (Nobel classification),fasting plasma glucose (FPG),and blood fat,and so on,in two groups,were observed and statistically analyzed.Results The difference of the Nobel classification,SBP and PP in two groups showed a statistical significance (P<0.05).While the difference of the age,gender,length of myocardial bridge,DBP,FPG,total cholesterol,low-density hpoprotein in two groups showed no statistical significance (P>0.05).A further regression analysis suggested that Nobel classification and PP had a correlation with the comphcation of stonosis before the myocardial bridge (r=3.0569,0.9740,P<0.05).Conclusions High blood pressure cases are liable to suffer from myocardial bridge.Myocardial bridges themselves trend to promote or accelerate the atherosclerotic stenosis of the coronary arteries before.them.The oppression degree of myocardial bridge and PP has a correlation with the complication of stenosis before the myocardial bridge,while has no correlations with age,gender,bloodfat,SBP,DBP,FPG,length of myocardial bridge,and so on.
9.Application of dextran sulfate pretreatment in animal model of umbilical cord blood transplantation
Jianqiu WU ; Zhijun JIN ; Yingsong XIANG ; Yunfang YANG ; Rujun YANG ; Ming YAO ;
Academic Journal of Second Military Medical University 2000;0(08):-
Objective: To better understand homing potentiality of hematopoietic stem cells (HSC) in human umbilial cord blood (UCB) and the mechanism of dextran sulfate (DS) mobilization. Methods: Sub lethally irradiated or DS pretreated severe combined immunodeficient (SCID) mice were transplanted with UCB, which was cryopreserved at -80℃.Human cells in recipient mice were detected by flow cytometry and CFU GM assay from each host organ. Results: In contrast with the controls, engraftment after irradiation or administration of DS resulted in a higher percentage of CD45 +,CD34 +,CD19 + cells produced in SCID mice. While comparison between the experimental groups, higher implantation level was obtained in the former if equivalent donor cells were used in both groups. Conclusion:DS is a safe and effective pretreatment, which can mobilize HSC, but also vocate niches for transplanted HSC homing. [
10.Preparation and identification of the monoclonal antibody against human dual-specificity protein phosphatase 18
Hongyan DU ; Xiaoyan YAO ; Xuexi YANG ; Yingsong WU ; Weiwen XU ; Ming LI
Cancer Research and Clinic 2009;21(4):219-221
Objective To prepare the human dual-specificity protein phosphatase18 (Dusp18) monoclonal antibodies (McAb) with high titer and specificity and identify its characterization, which is based on further studying Dusp18 function. Methods BALB/c mice were immunized with purified recombinant Dusp18 protein. Cell fusion was performed between mouse splenic cells and myeloma cells (SP2/0), and then the hybridoma cell lines secreting McAb against Dusp18 antigen were screened and cloned. The ascites were prepared and purified with Protein G affinity chromatography. The titer and subtypes of McAb against Dusp18 were identified and measured by ELISA and Western blotting analysis. Results Two hybridoma cell lines, F003 and F004, that stably secreted McAb against Dusp18 were successfully obtained, which belong to the subtypes of IgG1 and k light chain. The antibody titers in culture supematant were 1:5120 and1:10 240, and those in the ascites fluid were 1:25 600 and 1:51 200 respectively. Western blotting analysis and immunohistochemistry showed that the two antibodies can specifically bind with Dusp18 derived from human eucaryotic cells or tissue. Conclusion Two McAb against Dusp18 have been successfully prepared which can be used for further studying the biological properties of Dusp18 and reveal its relationship with tumorigenesis and development.