1.Spinal epidural abscess in children: report of five cases.
Chinese Journal of Contemporary Pediatrics 2009;11(11):941-942
Adolescent
;
Child
;
Epidural Abscess
;
diagnosis
;
surgery
;
Female
;
Humans
;
Male
;
Spinal Diseases
;
diagnosis
;
surgery
2.Clinical characteristics of pulmonary embolism and thrombolytic therapy in elderly patients
Shuhong MING ; Min YANG ; Tieying SUN
Chinese Journal of Geriatrics 2008;27(7):510-513
Objective To explore the elinical characteristics and the effects of thrombolytic and anti-coagulation therapy on pulmonary embolism(PE)in over 60-year-old patients. Methods The clinical findings,diagnostic techniques,effects of thrombolytic and anti coagulation therapy in 72 patients with PE aged over 60-year were analyzed retrospectively. Results Each one of 72 patients in this study suffered from two or more chronic diseases.Hypertension(56.9%)and deep venous thrombosis(DVT)in lower limbs(53.6%)were the most common thrombosis risk factors in the study.The clinical findings were atypical in elderly patients with PE.Different degree of dyspnea was the main characteristics(91.7%).Other findings were cough(30.6%),chest pain(27.8%),cyanosis (18.1%),faint(13.9%)and emptysis(12.5%).The objective signs showed edema of lower extremity (44.4%),moist rales(31.9%),P2 accentuation(18.1%),vascular murmur(5.6%).Blood gas analysis in 61 cases showed that 53 patients suffered from hypoxemia(86.9%)along with 37 cases of hypocapnia(60.7%).The alveolar-arterial oxygen gradient was increased in 27/31 cases(87.1%)and blood D-dipolymer was positive in 50/61 cases(82.0%).Spiral CT pulmonary angiogram(CTPA)in 62 cases and radioactive nuclear ventilation perfusion scan in 16 cases demonstrated PE in 58(93.5%) and 16(100%)patients respectively.The cure rate of thrombolytic therapy combined with anti-coagulation versus anti-coagulation therapy alone was 86.2%versus 30.2%(P=0.000).There was no haemorrhagia phenomenon during thrombolytic and anti-coagulation therapy. Conclusions The most common risk factors of PE in the elderly are hypertension and DVT in Iower limbs.The clinical symptoms are atypical and variable.Dyspnea is the main characteristics.Thrombolytic with anti-coagulation therapy is safe and effective,but anti-coagulation therapy alone has no benefit.
3.Neuroprotective effects of melatonin against hypoxic-ischemic brain damage in neonatal rats.
Zu-ming YANG ; Xing FENG ; Bin SUN
Chinese Journal of Pediatrics 2006;44(6):467-468
Animals
;
Animals, Newborn
;
Antioxidants
;
administration & dosage
;
pharmacology
;
Apoptosis
;
drug effects
;
Brain
;
drug effects
;
pathology
;
Disease Models, Animal
;
Female
;
Hypoxia-Ischemia, Brain
;
drug therapy
;
metabolism
;
pathology
;
Immunohistochemistry
;
Male
;
Melatonin
;
administration & dosage
;
pharmacology
;
Neuroprotective Agents
;
administration & dosage
;
pharmacology
;
Rats
;
Rats, Sprague-Dawley
4.The Effect of Tong-Xin Luo on Human Umbilical Vein Endothelial Cells Proliferation
Min YANG ; Ming SUN ; Hongyan ZHOU
Journal of Chinese Physician 2001;0(10):-
Objective To investigate the effects of Tong-Xin Luo (TXL) on the proliferation of human umbilical vein endothelial cells (ECV-304) cultured in vitro. Methods The TXL-contained serum was prepared, and ECV-304 were incubated with 5%, 10% and 15% serum containing TXL, respectively, for 4 hours and 8 hours. The cell morphology was observed and cell proliferation ability was determined by MTT. Results OD value of TXL group was higher than that in control group(P
5.Determination of hupzine A in Huperzia serrata by HPLC
Yuanming SUN ; Hongying YU ; Yuesheng YANG ; Jinyi YANG ; Ming ZHANG
Chinese Traditional and Herbal Drugs 1994;0(12):-
Object To compare the hupzine A (Hup A) in Huperzia serrata (Thunb.) Trev. obtained by different extracting methods and investigate the amount of alkaloids and the content of Hup A from different parts of the plants and from different places. Methods Using HPLC for the determination of Hup A. Results The content of Hup A in the stem and leaf is richer than that in the root. The content of Hup A from Guizhou, Guangdong and Anhui Provinces is 0.018%, 0.021% and 0.020% repectively; The difference of extract method of Hup A is no prominence. Conclusion The content of Hup A in the ground is richer than that of underground, and there are some difference in the content of Hup A obtained from different places.
6.Influence of cationic cyclopeptide on microstructure and permeability of Caco-2 cell membrane.
Xiao-Hui LI ; Ming-Ming CHANG ; Qing WANG ; Yu-Ming SUN ; Yang LIU
Acta Pharmaceutica Sinica 2014;49(7):1062-1068
The microstructure of cationic cyclopeptide (TD-34) treated Caco-2 cell membrane was observed, and we discussed the relationship between membrane structure and insulin transmembrane permeability. Atomic force microscope (AFM) was used to observe living cell membrane in air condition and tapping mode. Results showed that the surface of Caco-2 cell membrane treated with TD-34 lost its smoothness and nearly doubled its roughness. Apparent permeability coefficients (P(app)) of insulin in Caco-2 cell monolayers increased 2.5 times. In conclusion, AFM can be used to observe microstructure of cationic cyclopeptide treated cell membrane and cationic cyclopeptide enhanced insulin delivery across Caco-2 cell membrane by increasing membrane fluidity.
Caco-2 Cells
;
Cations
;
Cell Membrane
;
drug effects
;
Cell Membrane Permeability
;
drug effects
;
Humans
;
Insulin
;
metabolism
;
Membrane Fluidity
;
drug effects
;
Microscopy, Atomic Force
;
Peptides, Cyclic
;
pharmacology
7.The effects of Tong Xin-Luo on human umbilical vein endothelial cells impaired by lysophosphatidylcholine
Min YANG ; Jingfang LIU ; Ming SUN ; Huifang SHEN ; Hongyan ZHOU
Journal of Chinese Physician 2012;14(5):607-609
Objective To investigate the effects of Tong Xin-Luo on cultured human umbilical vein endothelial cells (HUVECs) impaired by Lysophosphatidylcholine.Methods The herbage-contained serum of TXL was prepared,HUVECs were cultured in vitro.The study was designated to 4 group:normal control,LPC group,TXL group,and TXL + LPC intervened group.The cell function was determined by cell morphology and MTT colorimetric assay.Results Compaired with normal control group (0.380 ±0.023 ),LPC ( 0.320 ± 0.024 ) could significantly decrease the cells activity,promote cells death ( P <0.05 ).After TXL intervened(0.424 ±0.034),cells activity was significantly increased,cells death was d significantly decreased( P <0.05 ).Conclusions Tong Xin-Luo could protect human umbilical vein endothelial cells function by against the LPC-induced damage.
8.Evaluation on safety and efficacy of tirofiban in patients with acute ST-elevation myocardial infarction undergoing emergency percutaneous coronary intervention
Liwei CHEN ; Ming YANG ; Kang GAO ; Yiguang SUN ; Ling HAN
Chinese Journal of Postgraduates of Medicine 2011;34(24):3-6
ObjectiveTo evaluate the safety and efficacy of tirofiba in the treatment of patients with acute ST-elevation myocardial infarction (STEMI) undergoing emergency percutaneous coronary intervention (PCI). MethodsA total of 158 patients with acute STEMI were randomly divided into tirofiban group 1 (59 cases, received tirofiban before PCI), tirofiban group 2 (56 cases, received tirofiban when PCI) and control group(43 cases, only received PCI). The coronary reperfusion flow(TIMI grade) of infarct related artery (IRA) after PCI, the resolution of the sum of ST segment elevation(sum STR) at 90 min after the procedure, the changes of myocardial enzyme at 6 h and 12 h afterwards, the left ventricular ejection fraction (LVEF) 1 week later, the major adverse cardiac events(MACE) within 30 d, bleeding and thrombocytopenia complications were analyzed and compared among the three groups. ResultsTIMI reperfusion grades in tirofiban group 1[98.3%(58/59 )]and tirofiban group 2[92.9%(52/56)]were higher than those in control group[60.5%(26/43)](P <0.05). The resolution of sum STR at 90 min after PCI in tirofiban group 1 [(89.3 ± 6.9)%]and tirofiban group 2[(82.4 + 7.3)%]was higher than that in control group[(65.6 +8.1 )%](P< 0.01 ),and there was significant difference between tirofiban group I and tirofiban group 2 (P<0.05 ). The occurrence of MACE within 30 d was lower in tirofiban group 1 and tirofiban group 2 than that in control group (P< 0.05). The level of CK-MB at 6 h and 12 h afterwards was lower in tirofiban group 1 than that in tirofiban group 2,and tirofiban group 2 was lower than control group (P< 0.05). LVEF 1 week later in tirofiban group 1[(56.2 + 6.4)%]was higher than that in tirofiban group 2[(51.1 + 4.9)%]and control group[(49.8 + 5.7)%](P < 0.05),but there was no significant difference between tirofiban group 2 and control group (P > 0.05). Although bleeding incidence in tirofiban group 1 and tirofiban group 2 was higher than that in control group, no severe bleeding and thrombocytopenia was observed. Conclusion Tirofiban can safely and effectively reduce the incidence of the ischemic events in the patients with acute STEM1 during preoperative of emergency PCI.
9.An epidemiologic study of diabetes mellitus in adolescents in Hanjiang district, Yangzhou
Yanling GAO ; Yan XU ; Jun ZHANG ; Feng SUN ; Ming YANG
Chinese Journal of Endocrinology and Metabolism 2008;24(6):643-644
The prevalence of diabetes mellitus and its relative risk factors in adolescents were investigated in Hanjiang district, Yangzhou. A total of 2 153 adolescents aged from 16 to 18 years old from 7 high schools were enrolled. The prevalence of diabetes mellitus and impaired fasting glucose was 6.13% and 16.12%, respectively. The independent risk factors are obesity or overweight.