Aim To study the effect of extracts of ginkgo biloba leaves(GbE) on apoptosis in neuronal cells. Methods Primary cerebral cultures from rat fetus were used to observe the activity of neuronal cells and to determine the release of LDH and DNA gel electrophoresis.Results GbE enhanced the activity of neuronal cells, decresed the release of LDH,and relieved the structural changes of nucleus and DNA fragmentation. Conclusion GbE can inhibit apoptosis in neuronal cells.

AimTo study the effect of extracts of ginkgo biloba leaves(GbE) on apoptosis in neuronal cells. Methods Primary cerebral cultures from rat fetus were used to observe the activity of neuronal cells and to determine the release of LDH and DNA gel electrophoresis. Results Gb E enhanced the activity of neuronal cells, decresed the release of LDH,and relieved the structural changes of nucleus and DNA fragmentation. Conclusion GbE can inhibit apoptosis in neuronal cells.

Computer-Assisted Instruction ; Education, Medical ; Internet ; Language ; Medicine, Chinese Traditional

Chromatography, Gas ; Humans ; Methylene Chloride ; urine

At present, a number of scientific research achievements has been formed. Scientific achievement is the crystallization of great efforts from scientific workers, and it's also the valuable treasure of human civilization. Standardization is an important way to promote the international communication of Chinese medicine, and it's significant in boosting China's scientific and technological progress, improving market competitiveness and promoting international trade. Transformation of scientific research to the guideline is not only beneficial to improving the technology content of the standard, but also to the conversion from scientific research achievements into productivity. Therefore, only by absorbing the advanced scientific and technological achievements, reproducing the theory of traditional Chinese medicine (TCM) and medical technology in standard form, can make TCM keep pace with the times. This study preliminarily explores for the method to transform scientific research achievements into guideline, in order to provide reference for the future technical specifications, thus to further the development of TCM.
Achievement ; Medicine, Chinese Traditional ; standards

ObjectiveTo evaluate the therapeutic effect of Salvia miltiorrhiza and ligustrazine as an adjuvant therapy in the patients with electric arcburns.MethodsFifty patients with electric arc burns were divided into 2 groups according to the random number table, with 25 in each group. The patients in the treatment group received intravenous infusion of 10 ml Salvia miltiorrhiza and ligustrazineinjection for 7 days in addition to the conventional therapies, and those in the control group only treated with conventional therapies.ResultsThe rates of wound healing in the treatment group were significantly improved at 7 days(25.7% ± 3.1%vs.21.3%± 3.0%;t=5.122,P=0.000) and 14 days(60.3% ± 8.6%vs.53.8% ± 10.8%;t=2.434,P=0.022) than the control group. While at 21 days, there was no significant difference between the two groups (99.4% ± 1.7%vs.98.7% ± 2.7%;t=1.163,P=0.265). The time to wound healing in the treatment group was significantly shorter than that in the control group (18.9 ± 2.5 dvs.20.3 ± 2.2 d;t=-1.216,P=0.020).ConclusionsSalvia miltiorrhiza and ligustrazine injections as an adjuvant therapy has a beneficial therapeuticeffect in patients with electric arc burns.

0.05). Conclusion:The rapid tooth movement is achieved impressively through dentoalveolar distraction osteogenesis, and tooth inclinations become largely associated with tooth movement.

By employing the mother-child and meridian relations between the spleen and the lung, this paper expanded on the therapeutic method of“banking up earth to generate metal” and its clinical practice in terms of excess and deficiency. After that, a modern research on the theory was made from the following 6 perspectives:refractory angina, asthma, bronchitis and reflux gastritis, pulmonary and digestive lymphatic system, pulmonary and digestive immune system, pulmonary and digestive endocrine system, pulmonary inflammatory disease and gastrointestinal flora system, and the influence of them on spleen-deficiency asthma rats airway inflammation, which fully demonstrated the importance of the classic traditional Chinese medicine (TCM) treatment principle of“banking up earth to generate metal” on the treatment of lung and digestive diseases.

Objective To investigate the effect of propofol on the response of spinal cord to pain stimulation induced by intraplantar injection of formalin Methods Thirty SD rats of both sexes weighing 200 250g were randomly divided into six groups Pain stimulation was produced by subcutaneous injection of formalin (2 5%100?l) into the plantar region of unilateral front paw Group F received intraplantar injection of formalin only (n=6);group FP received intraperitoneal propofol 100?g?kg -1 10min after formalin injection (n=6); group PF received formalin injection 10min after intraperitoneal propofol 100?g?kg -1 (n=6); group P received intraperitoneal propofol 100?g?kg -1 only; group FS received intraperitoneal normal saline 10ml?kg -1 10min after formalin injection; and group S received intraperitoneal normal saline 10ml?kg -1 only 1 h after last injection (intraperitoneal or intraplantar) the animals were anesthetized and cervical spinal cord (where sensory nerves from front paw enter) was removed and sliced and examined for fos expression in the spinal cord using fos immunohistochemistry technique Results After formalin injection large numbers of fos like immunoreactive neurons (FLINs) were found in the ipsilateral dorsal horn Most of FLINs were confined to the medial part of outer area of laminal I and II Intraperitoneal propofol injected either before or after formalin stimulation significantly suppressed fos expression in all laminal (P

Objective Due to the incompleteness and complexity of fault diagnosis for power transformers, a comprehensive rough-fuzzy scheme for solving fault diagnosis problems is presented. Fuzzy set theory is used both for representation of incipient faults' indications and producing a fuzzy granulation of the feature space. Rough set theory is used to obtain dependency rules that model indicative regions in the granulated feature space. The fuzzy membership functions corresponding to the indicative regions, modelled by rules, are stored as cases. Results Diagnostic conclusions are made using a similarity measure based on these membership functions. Each case involves only a reduced number of relevant features making this scheme suitable for fault diagnosis. Conclusion Superiority of this method in terms of classification accuracy and case generation is demonstrated.