Objective: To investigate the effects of bovine plasma fibronectin on the proliferation and differentiation of rat osteoblasts. Methods:Rat osteoblasts were cultured and identified. AlamarBlue were used to determine the effect of various concentration of bovine plasma fibronectin on the proliferation of the osteoblasts. ELISA method was used to determine the alkaline phosphatase activity of the cells. Results: Fibronectin stimulated the proliferation of rat osteoblasts at 40 ?g/ml (P

OBJECTIVE:To find out the main problems about quality supervision over Chinese herbal pieces and effective su-pervision strategies. MEDTHODS:First,static and dynamic models of the game between the enterprises engaged in the production and business of Chinese herbal pieces and local food and drug administration were respectively established,to which pure strategy Nash equilibrium analysis and mixed strategy Nash equilibrium analysis were made. Next,the model of the game between the supe-rior regulatory authorities and the basic-level regulatory authorities was established,to which mixed strategy analysis was made to find out the supervision factors relevant to the quality safety of Chinese herbal pieces. RESULTS & CONCLUSIONS:Based on the current situation of quality supervision over Chinese herbal pieces and the analysis results of the game models,it is suggested that at present the Chinese herbal medicine regulatory authorities of China should work hard to promote the reform of the supervision system,reduce supervision cost(including the guidance of the large-scale and centralized production of Chinese medicinal materi-als and unified appliance of approval number to the administration of Chinese herbal pieces),establish a systematic and comprehen-sive system of rewards and punishments,create a professional supervisor staff(including the strengthening of basic-level supervisor staff creation and the improvement of personnel's quality,the standardization of the law enforcement of regulatory authorities and the combination of proper power delegation to lower levels and strict supervision),and balance the relationship between the govern-ment and the public in supervision to increase the public's awareness of responsibility.

Objective To explore the changes and the clinical signficance in serum myocardial zymogram of patients with intracerebral be hemorrhage(ICH).Methods To estimate the serum myocardial zymogram.Serum aspartate aminotrasferase(AST),lactate dehychogenase (LDH),creatine kinas (CK) in patients with ICH were measured in 3 days,then to make comparison with control group.Results The serum myocardial zymogram in ICH group were higher than that of control group(P <0.05).There were different changes in serum myocardial zymogram of various parts.The serum myocardial zymogram in group with temporal lobe damage was higher than that of group without temporal lobe damage (P < 0.05).The serum myocardial zymogram of group with colliculus brain damage was higher than that of group without colliculus brain damage(P <0.05) ,the serum myocardial zymogram of group with disorder of consciousness was higher than that of group wakefulness(P < 0.05).Conclusion There are significant changes in the serum myocardial zymogram of patients with ICH.The incidence of the serum myocardial zymogram in ICH was close]y correlated to the severity area and part of ICH,consciousness disorder.To estimate serum zymogram is helpful to judge patients' state and prognosis.

Animals ; Cell Culture Techniques ; methods ; Cells, Cultured ; Cryopreservation ; Male ; Mice ; Spermatogonia ; cytology ; Stem Cells ; cytology

Objective To investigate the correlation between histone deacetylase 9 gene ( HDAC9 ) single nucleotide polymorphisms and ischemic stroke ( IS) subtypes. Methods A total of 202 patients with IS were enrolled prospectively. According to the Trial of Org 10172 in Acute Stroke Treatment ( TOAST) classification,the patients with IS were divided into large artery atherosclerotic stroke group (LAA,n=149) and the small-artery occlusive stroke group (SAO,n=53). A total of 201 age- and sex-matched healthy subjects over the same period were enrolled as the control group. Polymerase chain reaction-ligase detection reaction ( PCR-LDR ) was used to perform genotyping for HDAC9 gene rs11984041 and rs2107595 loci in all subjects. The correlation between genotype and IS was analyzed with multivariate logistic regression analysis. Results ( 1 ) In all the subjects detected, the genotype detected from rs11984041 loci was type CC,and 3 genotypes including CC,CT,and TT were detected from rs2107595 loci. (2) There were significant differences in dominance model (CT+TT,CC) genotypes and allele frequencies among the LAA, SAO, and control group dominance model ( CT +TT, CC ) and (χ2 =8. 635,P=0. 013, χ2 =10. 309,P=0. 006);and there were no significant differences in dominance model (CT+TT,CC) and allele frequencies among the 3 groups (after adjustment all P>0. 017). Compared with the control group,there were significant differences in the CC,CT,and TT genotypes and allele frequencies in the LAA group (χ2 =7. 446;P=0. 006). In the dominance models (CT+TT),there was significant difference in genotype frequencies between the LAA group ( 65. 1%,97/149 ) and the control group (52. 2%,105/201) (χ2 =5. 800;P=0. 016). (3) Multivariate logistic regression analysis showed that in addition to gender,hypertension,smoking,and high level of low-density lipoprotein,the dominance model CT+TT genotype was associated with the LAA type IS ( CT+TT genotype:OR,1. 909,95% CI 1. 055-3. 454,P =0. 033). Conclusion In addition to the risk factors for cerebrovascular disease such as gender,hypertension,smoking,and high level of low-density lipoprotein,HDAC9 gene polymorphisms may be associated with the LAA type IS. rs2107595 locus CT + TT genotype may be an independent risk factor for LAA type IS.

OBJECTIVE To make clear the germicidal efficacy of a large-sized pressure-stream disinfector for(bedclothes) used in the hospital.METHODS The carrier-ration sterilization experiment and local disinfection(experiment) were used to test the germicidal efficacy of pressure-stream disinfector for microorganism being on the contaminated bedclothes.RESULTS Operating the disinfector at the 105℃ for 5 minutes,its killing rate of Staphylococcus aureus,Escherichia coli and Candida albicans on bedclothes was 100%,the natural killing rate of(surface) of bedclothes exceeded 99.28%.CONCLUSIONS The pressure-stream disinfection is an excellently(effective),economical and environment protective method,it deserves to be used widely.

Objective　To construct an anti-LPS single chain phage antibody library in mice for further biomedical works. Methods　Total RNA was extracted from splenic cells for reverse transcription after BALB/C mice had been immunized with pure LPS for 4 weeks. The designed primers were used to amplify the variable region genes of both heavy and light chain (VH,VL) with polymerase chain reaction. The VH and VL were then conjugated to form a single chain of variable fragment (ScFv) by a linker. The ScFv was cut by NotⅠ and SfiⅠ and then ligated into a pCANTAB5E phagemid vector which was digested with the same restriction enzymes. The ligated vector was then tranfected into the competent E.coli TG1 cells. Four TG1 clones were randomly selected to detect the exotic DNA. Results　The titer of anti-LPS in murine sera was 1∶12 800. The concentration of total RNA was 12.3813 μg/ml. The length of the fragments were 340 bp for VH, 320 bp for VL and 800 bp for ScFv respectively. 1.9×107 clones were grown after transfection and one from the four randomly selected clones was identified to contain the exotic DNA. Conclusion　A 4.75×106 murine anti-LPS single chain phage antibody library was successfully constructed.

Animals ; Coronary Disease ; Diagnosis, Differential ; Disease Models, Animal ; Medicine, Chinese Traditional ; Yang Deficiency

Adenoma ; Adult ; Ear Neoplasms ; Ear, Middle ; pathology ; Female ; Humans

Adult ; Case-Control Studies ; Glutathione Peroxidase ; blood ; Humans ; Middle Aged ; Occupational Exposure ; Oxidation-Reduction ; Silicosis ; blood ; Superoxide Dismutase ; blood