1.Expression of Toll-like receptors signaling pathway in chronic rhinosinusitis with nasal polyps
Xin WANG ; Chunyuan ZHAO ; Ming LIU
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2009;(23):1059-1062
Objective:To investigate the expression of Toll-like receptors(TLRs)signaling pathway in chronic rhinosinusitis(CRS)with nasal polyps.Method:Gene microarray analysis was used to detect the expression of TLRs signaling pathway in CRS with nasal polyps.Result:Of 19 differentially expressed(two-fold changes),4genes were upregulated and 15 genes were downregulated.Conclusion:The differentially expressed genes in TLRs signaling pathway may exert its effect in the pathogenesis of CRS.In addition,the roles of TLR9 and its agonists need further study.
4.Mechanism and Chemical Basis of the Effect of Bacteria on Urinary Stone Formation
Huan-Xin YUAN ; Yi-Ming LIU ; Jian-Ming OUYANG ;
Microbiology 1992;0(03):-
There are two populations of bacteria to affect the formation of urinary stones in humanity. The first one can promote the formation of urinary stone by increasing urinary pH, decreasing concentration of urinary inhibitors , and damaging the protective urothelial glycosaminoglycan layer. The second inhibit the formation of urinary stones. These bacteria ( mainly the intestinal oxalate degrading bacteria such as Oxalobacter formigenes, lactic acid bacteria, Enterococcus faecalis etc) can decrease urinary oxalate concentration by regulating exogenous oxalate. The problems faced and the developing direction were also indicated.
5.Cloning of D-hydantoinase Gene from Pseudomonas and Its Expression in E.coli
Zhiqiang LI ; Jingjing LIU ; Zhuoyi HU ; Zhenghua WANG ; Xin MING
Journal of China Pharmaceutical University 2001;(3):227-230
AIM The purpose is to construct D-hydantoinase genetic engineering strain for the purpose of the industrial production of D-p-hydroxyphenylglycine. METHODS D-hydantoinase gene was created from Pseudomonas putida 9801 by PCR technique and inserted into pMD18-T vector. The recombinant plasmid was transformed into several Escherichia coli strains. The positive transformants with D-hydantoinase activity were obtained by the two step screening, digoxigenin DNA labeling in situ hybridization and D-hydantoinase activity assay. RESULTS The D-hydantoinase activity of the genetic engineering strain E.coli BL21/pMD-dht was 1700 U*L-1 and increased as high as 8 times compared with those of wild-type strain Pseudomonas putida 9801. The subunit molecular weight of recombinant D-hydantoinase was about 53 kDa measured by SDS-PAGE. The amount of the recombinant D-hydantoinase was about 20 percent of total bacterial soluble proteins. CONCLUSION The genetic engineering strain E.coli BL21/pMD-dht possesses the initial industrial production prospects.
6.Correlation of tacrolimus blood concentration and the levels of blood lipid and fasting glucose after renal transplantation
Xiangduan LIU ; Qingshan QU ; Xin JIANG ; Ming LI
Chinese Journal of Organ Transplantation 2013;(4):227-230
Objective To study the implact of tacrolimus blood concentration (Tac) after renal transplantation on blood lipid and fasting glucose (FBG) in recipients.Method The recipients who regularly given Tac + mycophenolate mofetil + prednisone after renal transplantation were chosen and those had normal preoperative levels of blood lipid and fasting glucose as subjects.The recipients were classied into high concentration group,normal concentration group and low concentration group by comparing Tac blood concentration of different postoperative periods with corresponding normal concentration range.The changes of biochemical indexes such as steady Tac valley blood concentration,blood lipid and FBG were monitored,and the differences in blood lipid and FBG levels at different periods among three concentration groups were compared.Result TG level was significantly higher (P<0.05),and HDL-C level was significantly lower in high concentration group than in normal and low concentration groups one month after operation (P<0.05).Three months after operation,TG and FBG levels were significantly higher in high concentration group than in normal and low concentration groups (P<0.05 and 0.01,respectively).Six months after operation,TC,TG and FBG levels were significantly higher in high concentration group than in normal and low concentration groups (P<0.05,0.01 and 0.05,respectively).There was no significant difference between normal and low concentration groups in various biochemical indexes at any time point (P>0.05).Conclusion The higher blood concentration of Tac and the longer used after renal transplantation,the easier it might cause drug-induced hyperlipidemia and diabetes.
7.Correlation of age with detection rate, grade and stage of prostate caner in patients with PSA 4-10 μg/L
Ming LIU ; Huimin HOU ; Hongxue SU ; Xin WANG ; Jianye WANG
Chinese Journal of Urology 2014;35(5):346-348
Objective To analyze the impact of age on the detection rate,grade and stage of prostate cancer in patients with prostate specific antigen (PSA) 4-10 μg/L.Methods A retrospective analysis was made in 426 patients with PSA 4-10 μg/L who underwent prostate biopsy and divided into 4 group according to their age less than 60 (n=33),60-69 (n=163),70-79 (n=202) and more than 80 (n=28) to compare the correlation between age and the cancer detective rate.Among them,115 patients,diagnosed as prostate cancer,were further divided into 4 group according to their age less than 60 (n =3),60-69 (n=32),70-79 (n=69) and more than 80 (n=11).The correlation between age and the Gleason score or clinical stage was analyzed in 115 prostate cancer patients.Results The detective rate in each group was 9.1% (3/33),19.6% (32/163),34.2% (69/202) and 39.3% (11/28).There was significant difference in prostate cancer detective rate among those groups.The cancer detective rate became significantly higher as the age increased (P<0.001).In the patients with prostate cancer,the number of patients with Gleason score more than 7 in each group was 1 (50.0%),11 (37.9%),30 (44.8%),7 (63.6%).No significant correlation was found between age and grade (P=0.292 4).And each group had cases with localized prostate cancer was 2 (100.0%),14 (60.9%),39 (78.0%),4 (66.7%),with locally advanced prostate cancer 0,5 (21.7%),7 (14.0%),1 (16.7%),with metastatic prostate cancer 0,4 (17.4%),4(8.0%),1 (16.7%).No significant difference was found between each group and clinical stage (P =0.101 6,P=0.285 2,P=0.349 1).Conclusions The prostate cancer detective rate significantly increases with the age increasing.There is no significant correlation between age and grade or clinical stage in the patients with PSA 4-10 μg/L.
8.A new tale of p21 activated protein kinase in the heart:pathophysiology to drug discovery
Shoubao WANG ; Hoyee TSUI ; Wei LIU ; Ming LEI ; Xin WANG
Chinese Journal of Pharmacology and Toxicology 2014;(4):475-483
CardiovascuIar disease is the number one cause for morbidity and mortaIity worIdwide. Possi-biIities for new therapies in the emerging fieId of cardiac signaIIing prompted extensive research on myocardiaI re-modeIIing over the past decades. In this review,we as-sembIe an overview of the recent findings on the muIti-functionaI enzyme,p21-activated kinase 1( Pak1),a member of a serine/ threonine protein kinase famiIy in the heart,particuIarIy its cardiac protective effects. We pres-ent a modeI for Pak1 signaIing that provides a mecha-nism for specificaIIy affecting cardiac ceIIuIar processes. We discuss its cardiac protective effects such as anti-hy-pertrophy,anti-ischaemic injury and roIe in maintaining ventricuIar Ca2+ homeostasis and eIectrophysioIogicaI stabiIity under physioIogicaI, β-adrenergic and hyper-trophic stress conditions.We aIso discuss the potentiaIs of Pak1 activation by naturaIIy occurring sphingosine and its anaIogues FTY720,and bioactive peptides designed to diminish Pak1 auto-inhibition as noveI thera-peutics for major cardiovascuIar diseases.
9.Clinical study on treatment of primary hypertension by niuhuang jiangya pill.
Sui-xin LIU ; Ming SUN ; You-fu LUO
Chinese Journal of Integrated Traditional and Western Medicine 2004;24(6):553-555
Adult
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Aged
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Antihypertensive Agents
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therapeutic use
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Capsules
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Drugs, Chinese Herbal
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therapeutic use
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Female
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Humans
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Hypertension
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drug therapy
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Male
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Middle Aged
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Phytotherapy
10.CT staging of esophageal carcinoma in 304 patients treated chiefly by radiotherapy
Ming LIU ; Caiying LI ; Xin WAN ; Junjie ZHANG ; Jun WAN
Chinese Journal of Radiation Oncology 1992;0(01):-
Objective To evaluate the feasibility of CT staging for esophageal cancer. Methods A retrospective analysis of 304 patients treated,from Jan. 1996 to Dec. 1998 chiefly with radiotherapy(126 conventional radiotherapy,55-65 Gy/27-35 fx/5.5-7.0 w;178 late-course accelerated hyperfractionated radiotherapy ,55-60 Gy/33-36 fx/ 4.5 -5.5 w)complete with CT data before treatment was done. The long survival was compared with pre-therapy CT findings and CT staging. ResultsThe survival rate of stage T1+T2 was very significantly different from those lesion with stage T3,T4(? 2=12.90,P0.05).The survival rates of patients positive for lymph nodes or distant metastasis were lower. Conclusion CT staging is quite optimal for non-operable esophageal cancer in clinical staging,as it is conducive to predict the prognosis.